Incidental Mutation 'R7526:Kif18b'
ID 583015
Institutional Source Beutler Lab
Gene Symbol Kif18b
Ensembl Gene ENSMUSG00000051378
Gene Name kinesin family member 18B
Synonyms 3000004C01Rik, N-8 kinesin
MMRRC Submission
Accession Numbers
Is this an essential gene? Essential (E-score: 1.000) question?
Stock # R7526 (G1)
Quality Score 225.009
Status Validated
Chromosome 11
Chromosomal Location 102905529-102925124 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to C at 102914667 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Valine at position 255 (I255V)
Ref Sequence ENSEMBL: ENSMUSP00000021311 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000021311]
AlphaFold Q6PFD6
Predicted Effect probably damaging
Transcript: ENSMUST00000021311
AA Change: I255V

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)
SMART Domains Protein: ENSMUSP00000021311
Gene: ENSMUSG00000051378
AA Change: I255V

DomainStartEndE-ValueType
KISc 7 361 1.47e-158 SMART
coiled coil region 368 404 N/A INTRINSIC
low complexity region 510 522 N/A INTRINSIC
low complexity region 676 682 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.0%
Validation Efficiency 100% (74/74)
Allele List at MGI
Other mutations in this stock
Total: 76 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700086D15Rik A T 11: 65,152,981 F85I unknown Het
Ankrd22 A T 19: 34,149,365 W22R possibly damaging Het
Aqr A T 2: 114,108,109 H1287Q probably damaging Het
Armh1 C A 4: 117,213,741 A396S probably benign Het
Atp8b5 A G 4: 43,366,609 E791G probably damaging Het
Bicd1 T G 6: 149,513,726 S646A possibly damaging Het
Bmpr1b A T 3: 141,856,599 Y276N probably damaging Het
Cadps2 T C 6: 23,496,851 H465R probably damaging Het
Card11 A T 5: 140,913,429 probably null Het
Ccdc9 A G 7: 16,282,400 L139P probably damaging Het
Cdk5rap3 A T 11: 96,909,945 M355K probably benign Het
Cnot2 A T 10: 116,507,080 V116E probably benign Het
Defb48 A G 14: 62,977,831 V32A possibly damaging Het
Dmkn A G 7: 30,777,651 D460G possibly damaging Het
Dmxl2 A G 9: 54,400,957 V2170A possibly damaging Het
Dnah1 A T 14: 31,287,876 F1912I possibly damaging Het
Dok3 C T 13: 55,527,493 V71I probably benign Het
Dzip3 A T 16: 48,975,474 F178Y probably damaging Het
Enpp1 T C 10: 24,674,410 probably null Het
Eogt A G 6: 97,113,952 F409L probably damaging Het
Erich6 A T 3: 58,630,689 L218H probably damaging Het
Fam186a G C 15: 99,941,915 I2149M possibly damaging Het
Fat1 G A 8: 45,023,427 V1837I probably damaging Het
Flrt3 T A 2: 140,660,206 T501S probably damaging Het
Fmn1 A C 2: 113,688,134 E1365D probably damaging Het
Fzd5 G T 1: 64,736,092 P170Q probably benign Het
Gm13101 A T 4: 143,965,817 C205S probably benign Het
Gm14326 G A 2: 177,946,505 H233Y probably damaging Het
Gm19410 G A 8: 35,790,612 V735M probably damaging Het
Gm21886 ACTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGG ACTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGG 18: 80,089,825 probably benign Het
Gm2832 A T 14: 41,280,962 I143L Het
Gm7534 T C 4: 134,200,073 probably null Het
Greb1 G A 12: 16,716,765 T344I probably benign Het
Grik2 A T 10: 49,523,822 Y271N possibly damaging Het
Grin3b A G 10: 79,973,051 N212D probably benign Het
Hgsnat A G 8: 25,971,049 L187P probably damaging Het
Hmcn1 A G 1: 150,656,573 I3152T probably damaging Het
Hsp90aa1 A T 12: 110,695,294 I96N unknown Het
Il18r1 T A 1: 40,471,772 L6I probably damaging Het
Ing3 G A 6: 21,953,799 V80I probably damaging Het
Kif2c A T 4: 117,182,432 N20K possibly damaging Het
Mfsd6l T C 11: 68,558,038 W572R probably damaging Het
Mybphl A G 3: 108,374,180 T71A probably benign Het
Myo7a G T 7: 98,085,448 T613K possibly damaging Het
Nfatc3 T C 8: 106,079,083 S195P probably damaging Het
Nrg1 T C 8: 31,818,323 N603S probably benign Het
Olfr1054 A T 2: 86,333,353 M1K probably null Het
Olfr1084 A G 2: 86,639,669 I13T possibly damaging Het
Olfr629 T C 7: 103,740,400 Y280C probably damaging Het
Parp8 T C 13: 116,894,805 E457G probably damaging Het
Pclo T C 5: 14,521,062 F154L probably benign Het
Pear1 A G 3: 87,752,568 S704P probably damaging Het
Pkib A G 10: 57,736,298 T92A probably benign Het
Pnpla7 A G 2: 24,998,666 R376G possibly damaging Het
Ptprd T A 4: 76,066,327 E527D probably benign Het
Pum1 T C 4: 130,747,026 V469A probably damaging Het
Rnf148 G A 6: 23,654,284 Q238* probably null Het
Scn9a A C 2: 66,483,646 N1909K probably benign Het
Sema3c A T 5: 17,727,596 H699L possibly damaging Het
Sema3f A T 9: 107,689,728 C201S probably damaging Het
Serinc2 T A 4: 130,258,790 D206V probably benign Het
Serping1 A T 2: 84,767,293 S415T probably benign Het
Sirpb1b A T 3: 15,548,872 L50Q probably damaging Het
Slc25a22 T C 7: 141,431,383 E262G probably benign Het
Slc5a8 A G 10: 88,902,491 I205M probably damaging Het
Sod2 G T 17: 13,008,031 probably benign Het
Tec T C 5: 72,786,019 I118V probably benign Het
Tenm3 A T 8: 48,287,812 V1212E probably damaging Het
Tex44 G A 1: 86,426,515 V49I probably benign Het
Tmem132d A T 5: 127,784,141 L972* probably null Het
Tpte G A 8: 22,325,547 probably null Het
Trim6 T A 7: 104,232,832 I456N probably damaging Het
Ubr4 G C 4: 139,422,417 V520L probably benign Het
Ubxn8 A T 8: 33,633,607 N101K probably benign Het
Vmn2r60 AG A 7: 42,195,734 probably null Het
Vmn2r83 A G 10: 79,491,558 T667A probably damaging Het
Other mutations in Kif18b
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00966:Kif18b APN 11 102914675 missense probably damaging 0.99
IGL01570:Kif18b APN 11 102912391 missense probably benign 0.04
IGL02965:Kif18b APN 11 102916512 start gained probably benign
IGL02997:Kif18b APN 11 102909084 missense probably damaging 1.00
IGL03135:Kif18b APN 11 102914260 missense probably damaging 1.00
IGL02988:Kif18b UTSW 11 102908320 missense probably damaging 1.00
R0541:Kif18b UTSW 11 102915175 missense probably damaging 0.98
R1446:Kif18b UTSW 11 102914699 missense probably damaging 0.99
R1674:Kif18b UTSW 11 102913060 missense probably benign 0.04
R1729:Kif18b UTSW 11 102915541 critical splice donor site probably null
R1784:Kif18b UTSW 11 102915541 critical splice donor site probably null
R2249:Kif18b UTSW 11 102912388 missense probably benign 0.01
R2291:Kif18b UTSW 11 102908270 missense probably damaging 1.00
R3723:Kif18b UTSW 11 102916276 missense probably damaging 1.00
R5496:Kif18b UTSW 11 102913742 missense possibly damaging 0.88
R5500:Kif18b UTSW 11 102915700 missense probably damaging 1.00
R5598:Kif18b UTSW 11 102908189 missense possibly damaging 0.89
R5820:Kif18b UTSW 11 102913048 missense probably benign 0.00
R5910:Kif18b UTSW 11 102913544 missense probably benign
R5912:Kif18b UTSW 11 102912991 missense probably benign
R6394:Kif18b UTSW 11 102914410 missense probably damaging 1.00
R6541:Kif18b UTSW 11 102914266 missense probably damaging 1.00
R6911:Kif18b UTSW 11 102916380 missense probably damaging 0.96
R7467:Kif18b UTSW 11 102916408 missense probably damaging 1.00
R7467:Kif18b UTSW 11 102912348 splice site probably null
R7581:Kif18b UTSW 11 102914722 missense probably damaging 1.00
R8314:Kif18b UTSW 11 102913074 missense probably benign
R8378:Kif18b UTSW 11 102916473 missense probably damaging 1.00
RF013:Kif18b UTSW 11 102912366 missense probably benign 0.00
Z1088:Kif18b UTSW 11 102908157 missense probably benign
Predicted Primers PCR Primer
(F):5'- GGGTCCAGGTGCTCATTTTC -3'
(R):5'- TTACTTGAGATGCTGACAAGAGG -3'

Sequencing Primer
(F):5'- CAGGTGCTCATTTTCTCTTCTTGGG -3'
(R):5'- GGCAACTGTAGCCGCACAC -3'
Posted On 2019-10-17