Mutagenetix > Incidental Mutations

Incidental Mutation 'R7532:Dclk3'

583376

Institutional Source

Beutler Lab

Gene Symbol

Dclk3

Ensembl Gene

ENSMUSG00000032500

Gene Name

doublecortin-like kinase 3

Synonyms

Click-I, -II related, Dcamkl3

MMRRC Submission

Accession Numbers

Genbank: NM_172928; MGI: 3039580

Is this an essential gene?

Possibly non essential (E-score: 0.277) question?

Stock #

R7532 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

111439081-111489118 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 111467528 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Alanine at position 47 (S47A)

Ref Sequence

ENSEMBL: ENSMUSP00000107510 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000111879]

AlphaFold

Q8BWQ5

Predicted Effect

probably benign
Transcript: ENSMUST00000111879
AA Change: S47A

PolyPhen 2 Score 0.029 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000107510
Gene: ENSMUSG00000032500
AA Change: S47A

Domain	Start	End	E-Value	Type
low complexity region	2	29	N/A	INTRINSIC
Pfam:DCX	115	177	2.9e-17	PFAM
low complexity region	200	218	N/A	INTRINSIC
low complexity region	267	277	N/A	INTRINSIC
low complexity region	479	496	N/A	INTRINSIC
S_TKc	514	771	1.63e-110	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a member of the protein kinase superfamily and the doublecortin family. Differently from the other two closely related family members (DCLK1 and DCLK2), the protein encoded by this gene contains only one N-terminal doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. The protein contains a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. [provided by RefSeq, Sep 2010]

Allele List at MGI

All alleles(3) : Targeted, other(2) Gene trapped(1)

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Add3	T	A	19: 53,232,158	I174K	probably damaging	Het
Ano2	A	T	6: 125,963,704	I597F	probably damaging	Het
Ap1g1	T	C	8: 109,860,164	V813A	probably damaging	Het
Bpnt1	A	T	1: 185,352,326	I207F	possibly damaging	Het
Brinp3	T	A	1: 146,901,401	W529R	probably damaging	Het
C2cd2l	C	T	9: 44,315,384	R355Q	probably benign	Het
Ccser1	T	A	6: 62,379,931	C784*	probably null	Het
Cdh7	A	T	1: 110,138,159	D721V	probably damaging	Het
Chd9	A	G	8: 90,994,565	I994V	unknown	Het
Cst13	T	G	2: 148,823,207	Y41D	probably benign	Het
Cyth2	C	T	7: 45,808,024	A342T	probably benign	Het
Dapk1	T	C	13: 60,730,886	L563P	probably damaging	Het
Dync1h1	C	A	12: 110,651,577	N3183K	probably benign	Het
Enpp1	C	T	10: 24,675,987	V165M	probably benign	Het
Ephx3	T	C	17: 32,188,789	N173S	possibly damaging	Het
Erc1	T	A	6: 119,779,631	D388V	probably benign	Het
Esp34	T	G	17: 38,559,620	V135G	possibly damaging	Het
Gfy	G	A	7: 45,178,037	P212S	probably damaging	Het
Gm5239	C	T	18: 35,536,742	R54C	probably benign	Het
Gtpbp3	T	C	8: 71,489,463	F113L	probably benign	Het
Hectd1	T	A	12: 51,790,450	D775V	probably damaging	Het
Ifrd2	T	G	9: 107,592,522	S431R	probably damaging	Het
Impg2	G	A	16: 56,267,180	A1121T	probably damaging	Het
Irx1	A	G	13: 71,960,195	F123L	possibly damaging	Het
Itgb6	C	T	2: 60,669,213	V79I	probably benign	Het
Kcnd3	G	A	3: 105,668,210	R550H	probably damaging	Het
Klhl9	A	T	4: 88,720,853	S384T	possibly damaging	Het
Kng2	T	C	16: 23,027,044		probably null	Het
Lipo3	T	C	19: 33,583,064	N67S	possibly damaging	Het
Mgst1	T	C	6: 138,153,506	S78P	probably benign	Het
Ms4a14	A	G	19: 11,303,959	Y412H	possibly damaging	Het
Mucl2	A	C	15: 103,896,052	I124S	unknown	Het
Myh3	A	G	11: 67,091,095	M806V	probably benign	Het
Nelfcd	T	C	2: 174,426,396	L501P	probably damaging	Het
Nlrp6	C	A	7: 140,925,184	P748Q	probably benign	Het
Olfr341	C	T	2: 36,480,126	M1I	probably null	Het
Olfr430	T	C	1: 174,070,098	S267P	probably benign	Het
Plxna2	G	A	1: 194,644,819	A354T	probably benign	Het
Prpf39	G	T	12: 65,053,371	V273L	probably benign	Het
Rad9a	T	C	19: 4,201,523		probably benign	Het
Rnf135	A	G	11: 80,198,906	D356G	probably benign	Het
Rragd	G	T	4: 33,004,166	A153S	possibly damaging	Het
Selenot	G	T	3: 58,585,232	V47L	probably benign	Het
Smc2	T	C	4: 52,451,013	L277P	probably damaging	Het
Sp7	A	G	15: 102,359,149	F92S	possibly damaging	Het
Spata1	A	T	3: 146,468,191	I380N	possibly damaging	Het
Spdye4b	G	T	5: 143,194,897	R39S	possibly damaging	Het
Stom	C	A	2: 35,321,577	R144L	possibly damaging	Het
Tex37	T	C	6: 70,913,637	K57R	probably benign	Het
Tsc22d1	T	C	14: 76,416,046		probably benign	Het
Unc13b	A	G	4: 43,249,565	T998A	probably benign	Het
Vcl	C	A	14: 21,029,324	A965D	probably damaging	Het
Vmn1r69	A	T	7: 10,580,354	V150D	probably damaging	Het
Vmn1r90	T	G	7: 14,561,264	N303T	possibly damaging	Het
Vmn2r69	A	T	7: 85,410,414	M429K	probably benign	Het
Vmn2r8	T	A	5: 108,802,240	Y247F	probably benign	Het
Washc5	A	T	15: 59,367,411	S168T	possibly damaging	Het

Other mutations in Dclk3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02027:Dclk3	APN	9	111467843	nonsense	probably null
IGL02125:Dclk3	APN	9	111469107	missense	probably damaging	1.00
IGL02547:Dclk3	APN	9	111469023	missense	probably damaging	1.00
IGL03393:Dclk3	APN	9	111488673	utr 3 prime	probably benign
G1citation:Dclk3	UTSW	9	111439337	missense	probably benign	0.03
IGL02984:Dclk3	UTSW	9	111488575	missense	probably damaging	1.00
R0109:Dclk3	UTSW	9	111467670	missense	possibly damaging	0.93
R0109:Dclk3	UTSW	9	111467670	missense	possibly damaging	0.93
R0238:Dclk3	UTSW	9	111482628	missense	probably damaging	0.99
R0238:Dclk3	UTSW	9	111482628	missense	probably damaging	0.99
R0432:Dclk3	UTSW	9	111484935	missense	probably damaging	1.00
R0440:Dclk3	UTSW	9	111469163	missense	probably damaging	1.00
R0530:Dclk3	UTSW	9	111482721	missense	probably damaging	1.00
R1024:Dclk3	UTSW	9	111469070	missense	possibly damaging	0.95
R1443:Dclk3	UTSW	9	111469020	missense	probably benign	0.01
R1474:Dclk3	UTSW	9	111469236	missense	probably benign	0.43
R1479:Dclk3	UTSW	9	111468546	missense	probably benign
R1482:Dclk3	UTSW	9	111467820	missense	possibly damaging	0.90
R1543:Dclk3	UTSW	9	111468054	missense	probably benign	0.04
R1552:Dclk3	UTSW	9	111488579	missense	probably damaging	1.00
R1559:Dclk3	UTSW	9	111469208	missense	probably damaging	1.00
R2011:Dclk3	UTSW	9	111468354	missense	probably benign	0.00
R2369:Dclk3	UTSW	9	111488542	missense	probably benign	0.16
R4111:Dclk3	UTSW	9	111469080	missense	probably damaging	0.99
R4510:Dclk3	UTSW	9	111467992	missense	probably benign	0.01
R4511:Dclk3	UTSW	9	111467992	missense	probably benign	0.01
R4592:Dclk3	UTSW	9	111467895	missense	probably damaging	1.00
R4604:Dclk3	UTSW	9	111469185	missense	probably damaging	1.00
R4857:Dclk3	UTSW	9	111468648	missense	probably benign
R4932:Dclk3	UTSW	9	111468042	missense	possibly damaging	0.56
R5045:Dclk3	UTSW	9	111467788	missense	probably damaging	0.99
R5233:Dclk3	UTSW	9	111468681	missense	probably benign
R5338:Dclk3	UTSW	9	111469059	missense	possibly damaging	0.95
R5463:Dclk3	UTSW	9	111469260	missense	probably benign	0.26
R6822:Dclk3	UTSW	9	111439337	missense	probably benign	0.03
R6995:Dclk3	UTSW	9	111467700	missense	possibly damaging	0.88
R7187:Dclk3	UTSW	9	111484996	missense	probably damaging	1.00
R7534:Dclk3	UTSW	9	111468218	missense	probably benign
R7734:Dclk3	UTSW	9	111469095	missense	probably damaging	1.00
R8326:Dclk3	UTSW	9	111467534	missense	probably damaging	0.96
R8372:Dclk3	UTSW	9	111485013	missense	probably damaging	0.98
R8388:Dclk3	UTSW	9	111482745	missense	probably damaging	1.00
R8423:Dclk3	UTSW	9	111468719	missense	possibly damaging	0.93
R8493:Dclk3	UTSW	9	111468147	missense	probably benign	0.06
R9013:Dclk3	UTSW	9	111468498	missense	probably benign	0.31
R9114:Dclk3	UTSW	9	111488615	missense	probably benign	0.06
R9338:Dclk3	UTSW	9	111439305	missense	unknown
R9412:Dclk3	UTSW	9	111482751	critical splice donor site	probably null
X0020:Dclk3	UTSW	9	111485075	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTGGCTAGCTGTTCTGTGAC -3'
(R):5'- CTCAAAGGTCTGCACTGAGC -3'

Sequencing Primer
(F):5'- GTGACCGAGTTACACTTCCAG -3'
(R):5'- AGGAGCAGGGTGGCCTTAC -3'

Posted On

2019-10-17