Mutagenetix > Incidental Mutation

Incidental Mutation 'R7534:Vmn2r8'

583445

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r8

Ensembl Gene

ENSMUSG00000090961

Gene Name

vomeronasal 2, receptor 8

Synonyms

EG627479

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R7534 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

108797193-108808754 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 108802174 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Phenylalanine at position 269 (Y269F)

Ref Sequence

ENSEMBL: ENSMUSP00000126953 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172140]

AlphaFold

L7N472

Predicted Effect

possibly damaging
Transcript: ENSMUST00000172140
AA Change: Y269F

PolyPhen 2 Score 0.942 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000126953
Gene: ENSMUSG00000090961
AA Change: Y269F

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	419	1.1e-28	PFAM
Pfam:NCD3G	507	561	8.2e-18	PFAM
Pfam:7tm_3	594	829	1.1e-54	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (48/48)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310035C23Rik	G	A	1: 105,741,023	R993Q	probably benign	Het
Aif1	A	C	17: 35,171,414	M120R	possibly damaging	Het
Ank2	T	C	3: 126,934,333		probably null	Het
Ankrd11	A	T	8: 122,894,410	I901N	probably damaging	Het
Apc	T	C	18: 34,316,962	S2304P	probably damaging	Het
AW551984	A	T	9: 39,591,481	C632S	probably benign	Het
Bicra	T	C	7: 15,971,935	N1527S	probably damaging	Het
Cacna1d	A	G	14: 30,079,362	L1363P	probably damaging	Het
Cacna1g	G	A	11: 94,411,078	P2037S	probably benign	Het
Cdc27	A	G	11: 104,508,414	L724S	probably damaging	Het
Cttnbp2	C	T	6: 18,420,765		probably null	Het
Dchs1	G	A	7: 105,772,373	A280V	probably benign	Het
Dclk3	G	A	9: 111,468,218	G277R	probably benign	Het
Ddr1	A	G	17: 35,682,622		probably null	Het
Dnah7c	A	C	1: 46,770,067	D3515A	probably damaging	Het
Elovl4	G	A	9: 83,790,119	T49M	probably damaging	Het
Fam13b	A	T	18: 34,498,007	F40I	probably damaging	Het
Fancd2os	T	C	6: 113,597,640	H135R	probably benign	Het
Fer1l6	A	G	15: 58,638,026	I1446V	probably damaging	Het
Fyb2	T	A	4: 104,999,348	Y584*	probably null	Het
Galnt16	A	T	12: 80,597,135	L442F	probably damaging	Het
Garem1	T	A	18: 21,299,916		probably benign	Het
Ggta1	C	T	2: 35,402,428	R289Q	probably damaging	Het
Gm11564	T	C	11: 99,815,521	T28A	unknown	Het
Hcn1	A	G	13: 117,975,425	T642A	unknown	Het
Hhat	A	T	1: 192,726,304	L173H	probably damaging	Het
Hook1	T	G	4: 96,017,597	I585S	probably benign	Het
Ighg1	A	T	12: 113,329,729	S114T		Het
Kmt2d	G	A	15: 98,852,018	P2598L	unknown	Het
Macc1	A	T	12: 119,447,519	H674L	probably benign	Het
Mypn	T	C	10: 63,193,131	E51G	probably benign	Het
Olfr1012	A	C	2: 85,759,459	L306V	probably benign	Het
Olfr134	T	A	17: 38,175,297	M71K	probably benign	Het
Olfr862	A	G	9: 19,884,176	V43A	probably benign	Het
Pnliprp2	T	A	19: 58,775,142	S408T	probably benign	Het
Ppara	A	C	15: 85,777,726	Y56S	probably benign	Het
Ppp1r1a	A	G	15: 103,532,389	C114R	probably benign	Het
Pycard	C	T	7: 127,993,485	V57I	probably damaging	Het
Rin3	T	G	12: 102,350,941	Y143D	unknown	Het
Slc12a7	A	G	13: 73,764,068		probably benign	Het
Slitrk3	C	T	3: 73,050,107	R444H	probably damaging	Het
Ttf2	C	T	3: 100,950,412		probably null	Het
Ufsp2	T	A	8: 45,980,324	I25N	probably benign	Het
Uqcrc2	A	G	7: 120,641,689	T115A	possibly damaging	Het
Vmn2r43	T	A	7: 8,255,231	R328*	probably null	Het
Zbtb5	T	C	4: 44,995,030	Y118C	probably damaging	Het
Zeb1	T	C	18: 5,766,611	V374A	probably damaging	Het

Other mutations in Vmn2r8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02957:Vmn2r8	APN	5	108802225	missense	probably benign	0.01
R0324:Vmn2r8	UTSW	5	108797941	splice site	probably null
R0335:Vmn2r8	UTSW	5	108797451	splice site	probably null
R0394:Vmn2r8	UTSW	5	108802072	missense	probably benign	0.12
R0501:Vmn2r8	UTSW	5	108803183	missense	probably benign	0.03
R0615:Vmn2r8	UTSW	5	108799329	missense	probably damaging	1.00
R0678:Vmn2r8	UTSW	5	108800546	missense	probably benign	0.00
R1167:Vmn2r8	UTSW	5	108803176	missense	probably benign	0.01
R1187:Vmn2r8	UTSW	5	108803219	nonsense	probably null
R1406:Vmn2r8	UTSW	5	108802368	missense	probably benign
R1406:Vmn2r8	UTSW	5	108802368	missense	probably benign
R1451:Vmn2r8	UTSW	5	108798067	missense	probably damaging	1.00
R1535:Vmn2r8	UTSW	5	108802174	missense	probably damaging	1.00
R1795:Vmn2r8	UTSW	5	108803106	missense	probably benign
R1874:Vmn2r8	UTSW	5	108802418	missense	possibly damaging	0.74
R1908:Vmn2r8	UTSW	5	108797570	missense	probably benign	0.03
R1925:Vmn2r8	UTSW	5	108802153	missense	probably damaging	0.97
R1960:Vmn2r8	UTSW	5	108799286	missense	probably damaging	0.99
R1961:Vmn2r8	UTSW	5	108798095	missense	probably benign	0.45
R1967:Vmn2r8	UTSW	5	108802383	missense	probably benign	0.01
R2095:Vmn2r8	UTSW	5	108808621	missense	possibly damaging	0.94
R2159:Vmn2r8	UTSW	5	108802303	missense	probably benign	0.22
R4240:Vmn2r8	UTSW	5	108797503	missense	probably damaging	0.99
R4581:Vmn2r8	UTSW	5	108801704	missense	probably benign	0.03
R4744:Vmn2r8	UTSW	5	108808581	missense	probably benign	0.00
R4755:Vmn2r8	UTSW	5	108801700	missense	probably benign	0.03
R4917:Vmn2r8	UTSW	5	108797398	missense	probably damaging	1.00
R4957:Vmn2r8	UTSW	5	108799263	missense	probably benign	0.16
R5141:Vmn2r8	UTSW	5	108808706	missense	probably damaging	0.96
R5481:Vmn2r8	UTSW	5	108801770	missense	probably benign	0.09
R5571:Vmn2r8	UTSW	5	108802240	missense	probably damaging	1.00
R5624:Vmn2r8	UTSW	5	108802459	missense	probably damaging	0.99
R6003:Vmn2r8	UTSW	5	108797382	missense	probably damaging	1.00
R6243:Vmn2r8	UTSW	5	108799345	missense	probably benign	0.01
R6265:Vmn2r8	UTSW	5	108808597	missense	probably benign
R6315:Vmn2r8	UTSW	5	108801891	missense	probably benign
R6413:Vmn2r8	UTSW	5	108801723	missense	probably benign	0.09
R7120:Vmn2r8	UTSW	5	108808638	missense	possibly damaging	0.56
R7406:Vmn2r8	UTSW	5	108800576	missense	probably benign	0.00
R7409:Vmn2r8	UTSW	5	108808583	nonsense	probably null
R7489:Vmn2r8	UTSW	5	108797656	missense	possibly damaging	0.95
R7532:Vmn2r8	UTSW	5	108802240	missense	probably benign	0.22
R7739:Vmn2r8	UTSW	5	108802177	missense	probably damaging	1.00
R8099:Vmn2r8	UTSW	5	108801834	missense	probably benign
R8245:Vmn2r8	UTSW	5	108798070	missense	probably damaging	1.00
R8711:Vmn2r8	UTSW	5	108798096	missense	possibly damaging	0.89
R8781:Vmn2r8	UTSW	5	108797731	missense	possibly damaging	0.95
R8874:Vmn2r8	UTSW	5	108808751	missense	probably damaging	1.00
R8927:Vmn2r8	UTSW	5	108802265	missense
R8928:Vmn2r8	UTSW	5	108802265	missense
R9288:Vmn2r8	UTSW	5	108802319	missense	probably benign	0.39
R9596:Vmn2r8	UTSW	5	108799330	missense	possibly damaging	0.94
R9652:Vmn2r8	UTSW	5	108803241	missense	probably benign	0.18
Z1088:Vmn2r8	UTSW	5	108801998	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CAATCTCACTGTGGTGGTGTTC -3'
(R):5'- TGCCATGGTCTCCTTGATG -3'

Sequencing Primer
(F):5'- GTGGTGTTCAAAAGTAACAGTCCC -3'
(R):5'- AGGACTGGTCATTTCAGATGATGAC -3'

Posted On

2019-10-17