Mutagenetix > Incidental Mutations

Incidental Mutation 'R7536:Gpr89'

583573

Institutional Source

Beutler Lab

Gene Symbol

Gpr89

Ensembl Gene

ENSMUSG00000028096

Gene Name

G protein-coupled receptor 89

Synonyms

4933412D19Rik, SH120

MMRRC Submission

Accession Numbers

Is this an essential gene?

Possibly non essential (E-score: 0.363) question?

Stock #

R7536 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

96868281-96905346 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 96890893 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Leucine at position 149 (R149L)

Ref Sequence

ENSEMBL: ENSMUSP00000029738 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029738] [ENSMUST00000154750]

AlphaFold

Q8BS95

Predicted Effect

probably damaging
Transcript: ENSMUST00000029738
AA Change: R149L

PolyPhen 2 Score 0.961 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000029738
Gene: ENSMUSG00000028096
AA Change: R149L

Domain	Start	End	E-Value	Type
transmembrane domain	4	26	N/A	INTRINSIC
transmembrane domain	38	60	N/A	INTRINSIC
transmembrane domain	75	97	N/A	INTRINSIC
transmembrane domain	110	127	N/A	INTRINSIC
Pfam:GPHR_N	140	207	1.1e-31	PFAM
Pfam:ABA_GPCR	276	446	4.9e-46	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000154750

SMART Domains

Protein: ENSMUSP00000116016
Gene: ENSMUSG00000028096

Domain	Start	End	E-Value	Type
transmembrane domain	4	26	N/A	INTRINSIC
transmembrane domain	38	60	N/A	INTRINSIC
transmembrane domain	75	97	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700014D04Rik	C	T	13: 59,741,742	V755M	probably damaging	Het
3632451O06Rik	G	T	14: 49,774,246		probably null	Het
Acta2	G	A	19: 34,252,531	T8I	probably benign	Het
AI597479	C	G	1: 43,111,345	A205G	possibly damaging	Het
Akr1c14	T	A	13: 4,063,690	V74E	probably damaging	Het
Bbs9	A	T	9: 22,670,800	Q596L	probably damaging	Het
Bub3	A	G	7: 131,568,703	D318G	probably damaging	Het
Cela1	A	G	15: 100,675,364	V248A	probably damaging	Het
Cer1	C	A	4: 82,884,968	R39L	probably benign	Het
Clec16a	A	G	16: 10,638,844	T624A	possibly damaging	Het
Coro1c	C	A	5: 113,845,289	G393W	probably damaging	Het
Crym	A	G	7: 120,201,108	L97P	probably damaging	Het
Cyp2a5	T	G	7: 26,840,478	L317R	probably damaging	Het
Cyp2j6	C	A	4: 96,535,537	G198V	probably damaging	Het
Dnajb6	A	G	5: 29,757,806	E238G	possibly damaging	Het
Dnhd1	C	A	7: 105,709,561	T3419K	probably damaging	Het
Dpep3	A	T	8: 105,977,400	I262K	probably damaging	Het
Dscam	T	G	16: 96,641,026		probably null	Het
Farsb	A	G	1: 78,443,754	V500A	possibly damaging	Het
Fbxl20	A	T	11: 98,095,383	C136*	probably null	Het
Fbxo43	G	T	15: 36,161,851	D403E	probably benign	Het
Frem1	A	G	4: 82,956,195	S1397P	probably damaging	Het
Fut8	A	G	12: 77,475,078	Y497C	probably damaging	Het
Gbp3	A	T	3: 142,566,395	R219S	probably damaging	Het
Gm14295	T	A	2: 176,810,929	H737Q	possibly damaging	Het
Greb1	A	T	12: 16,682,185	Y1592N	probably damaging	Het
Gria4	A	C	9: 4,464,298	Y555D	probably damaging	Het
Hspa1b	T	A	17: 34,958,875	T45S	possibly damaging	Het
Kif5b	A	T	18: 6,216,235	N571K	probably benign	Het
Mapkbp1	T	A	2: 120,018,585	M694K	probably damaging	Het
Med24	G	A	11: 98,712,621	H439Y	possibly damaging	Het
Mgl2	G	T	11: 70,137,007	R347L	probably benign	Het
Mms22l	T	A	4: 24,581,240	L850Q	probably damaging	Het
Mrpl51	T	C	6: 125,192,567	V44A	possibly damaging	Het
Mylk3	T	C	8: 85,353,604	I485V	probably benign	Het
Olfr1116	C	T	2: 87,269,599	Q273*	probably null	Het
Olfr868	C	A	9: 20,101,530	T257K	probably damaging	Het
Pde4dip	A	G	3: 97,757,244	L434P	probably damaging	Het
Pla2g4a	T	C	1: 149,880,017	Y223C	probably damaging	Het
Plcl1	C	T	1: 55,713,481	Q995*	probably null	Het
Pls1	C	T	9: 95,762,057	C462Y	probably damaging	Het
Ppp6r3	T	C	19: 3,507,341	E249G	possibly damaging	Het
Prb1	T	A	6: 132,207,221	N483I	unknown	Het
R3hdm1	A	G	1: 128,182,211		probably null	Het
Rasgrp3	T	C	17: 75,514,133	F445S	probably damaging	Het
Rnf39	G	T	17: 36,943,117	L10F	probably damaging	Het
Rnh1	T	C	7: 141,160,812	D410G	possibly damaging	Het
Sfi1	ACA	ACATCTTCCCAAAGCCAGTCA	11: 3,153,382		probably benign	Het
Sh3bp2	A	G	5: 34,543,557	T35A	probably benign	Het
Skint6	T	A	4: 112,811,547		probably null	Het
Slc13a1	A	T	6: 24,100,331	D384E	probably damaging	Het
Slc27a6	A	G	18: 58,556,626	T55A	probably damaging	Het
Slc46a2	G	A	4: 59,914,141	Q261*	probably null	Het
St7	C	G	6: 17,886,020	P327R	probably damaging	Het
Stx1a	T	C	5: 135,049,840	I268T	probably damaging	Het
Tcof1	G	C	18: 60,829,051	A702G	possibly damaging	Het
Tgm7	T	A	2: 121,096,397	R424*	probably null	Het
Tmem144	A	T	3: 79,827,657	N151K	probably benign	Het
Tmem145	T	C	7: 25,307,869	S171P	probably damaging	Het
Traf2	A	G	2: 25,537,106	Y78H	possibly damaging	Het
Tsc22d1	A	G	14: 76,504,763	Y16C	probably benign	Het
Ttc39b	A	T	4: 83,239,978	Y503*	probably null	Het
Ttn	T	C	2: 76,717,337	D32163G	probably benign	Het
Uba6	A	T	5: 86,124,332	S833T	probably benign	Het
Wdtc1	A	G	4: 133,295,250	L595P	probably damaging	Het

Other mutations in Gpr89

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00421:Gpr89	APN	3	96898523	missense	probably damaging	1.00
IGL00757:Gpr89	APN	3	96871534	missense	probably benign	0.00
IGL01114:Gpr89	APN	3	96893549	missense	probably damaging	0.98
IGL02417:Gpr89	APN	3	96897425	nonsense	probably null
explorer	UTSW	3	96880069	splice site	probably null
R0207:Gpr89	UTSW	3	96871480	missense	probably damaging	0.99
R0650:Gpr89	UTSW	3	96897324	splice site	probably benign
R0704:Gpr89	UTSW	3	96880168	critical splice acceptor site	probably null
R1496:Gpr89	UTSW	3	96905210	missense	probably benign	0.00
R1869:Gpr89	UTSW	3	96875659	missense	probably benign	0.16
R1913:Gpr89	UTSW	3	96875633	missense	possibly damaging	0.91
R2264:Gpr89	UTSW	3	96872515	missense	probably damaging	0.99
R2276:Gpr89	UTSW	3	96897427	missense	probably damaging	1.00
R3822:Gpr89	UTSW	3	96892944	missense	probably benign	0.03
R3922:Gpr89	UTSW	3	96890899	missense	probably damaging	1.00
R4984:Gpr89	UTSW	3	96905196	missense	probably benign	0.02
R5761:Gpr89	UTSW	3	96892880	missense	probably damaging	1.00
R6185:Gpr89	UTSW	3	96890833	missense	probably damaging	0.99
R7063:Gpr89	UTSW	3	96875698	missense	probably damaging	1.00
R7164:Gpr89	UTSW	3	96871398	missense	probably benign	0.04
R7172:Gpr89	UTSW	3	96880069	splice site	probably null
R7215:Gpr89	UTSW	3	96880088	missense	probably damaging	1.00
R7708:Gpr89	UTSW	3	96880625	missense	possibly damaging	0.81
R7849:Gpr89	UTSW	3	96871490	nonsense	probably null
R8273:Gpr89	UTSW	3	96905189	missense	probably benign
RF019:Gpr89	UTSW	3	96905193	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- ACACTGTTGTCCACTGGAAAG -3'
(R):5'- AGGCAAAGATCAGCAGCCTG -3'

Sequencing Primer
(F):5'- ATGCCTGTAATCCCAGTGTTCAGG -3'
(R):5'- GTAACTCCAGTTCCAGGGAATTC -3'

Posted On

2019-10-17