Mutagenetix > Incidental Mutation

Incidental Mutation 'R7537:Zranb3'

583627

Institutional Source

Beutler Lab

Gene Symbol

Zranb3

Ensembl Gene

ENSMUSG00000036086

Gene Name

zinc finger, RAN-binding domain containing 3

Synonyms

4933425L19Rik

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.211) question?

Stock #

R7537 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

127954184-128103047 bp(-) (GRCm38)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to T at 128032847 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000083806 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086614] [ENSMUST00000112538]

AlphaFold

Q6NZP1

Predicted Effect

probably null
Transcript: ENSMUST00000086614

SMART Domains

Protein: ENSMUSP00000083806
Gene: ENSMUSG00000036086

Domain	Start	End	E-Value	Type
DEXDc	33	214	3.37e-19	SMART
HELICc	352	435	3.79e-13	SMART
ZnF_RBZ	619	643	6.93e-5	SMART
HNHc	985	1036	5.64e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000112538

SMART Domains

Protein: ENSMUSP00000108157
Gene: ENSMUSG00000036086

Domain	Start	End	E-Value	Type
Pfam:SNF2_N	40	98	6.6e-12	PFAM

Meta Mutation Damage Score

0.9502

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.9%

Validation Efficiency

100% (63/63)

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca4	T	C	3: 122,173,988	L2229P	possibly damaging	Het
Acaca	T	A	11: 84,260,634	M786K	probably damaging	Het
Acta2	G	A	19: 34,252,531	T8I	probably benign	Het
Adap1	T	G	5: 139,293,173	E117D	possibly damaging	Het
Ap5z1	T	C	5: 142,477,298	S746P	probably benign	Het
Appl2	A	G	10: 83,617,428	I208T	possibly damaging	Het
Astn1	G	A	1: 158,667,638		probably null	Het
Astn1	A	G	1: 158,505,386	E346G	possibly damaging	Het
Atp23	T	A	10: 126,868,725	I180L	unknown	Het
Bean1	CT	C	8: 104,182,032		probably null	Het
Cdh23	A	T	10: 60,384,945	I1340N	probably benign	Het
Cdh8	G	A	8: 99,098,885	Q493*	probably null	Het
Ces1g	T	C	8: 93,319,827	I357V	probably benign	Het
Ddx46	A	G	13: 55,650,478	D226G	probably damaging	Het
Eea1	C	T	10: 95,994,905	Q143*	probably null	Het
Erlin2	G	T	8: 27,031,772		probably null	Het
Fat3	T	A	9: 15,938,319	D3929V	probably damaging	Het
Flt3	T	C	5: 147,334,437	D898G	probably damaging	Het
Gm16519	A	G	17: 70,929,356	N100S	probably benign	Het
Gnl1	A	G	17: 35,988,536	H533R	probably damaging	Het
Gphn	A	G	12: 78,504,680	T301A	possibly damaging	Het
Herc2	C	T	7: 56,219,779	R4295*	probably null	Het
Insm2	T	C	12: 55,599,518	S16P	possibly damaging	Het
Jak2	C	T	19: 29,298,637	T778I	probably benign	Het
Lrriq3	A	G	3: 155,101,097	T128A	probably damaging	Het
Lst1	A	G	17: 35,186,944		probably null	Het
Magi1	G	T	6: 93,708,110	Y762*	probably null	Het
Man2b1	T	A	8: 85,090,965	C358*	probably null	Het
Mga	T	C	2: 119,935,551	V1432A	probably damaging	Het
Mmp1a	TG	TGG	9: 7,465,083		probably null	Het
Morc2a	C	T	11: 3,683,566	Q587*	probably null	Het
Mrc2	T	C	11: 105,292,797	I4T	probably benign	Het
Muc16	C	A	9: 18,638,135	V5621F	probably benign	Het
Myl10	G	C	5: 136,697,971	V70L	probably benign	Het
Myo3b	A	G	2: 70,217,169	R340G	probably benign	Het
Nipal3	T	C	4: 135,490,937	Y34C	probably damaging	Het
Nktr	T	A	9: 121,749,279	D804E	unknown	Het
Nlrp4b	A	G	7: 10,714,889	M340V	probably benign	Het
Olfr1039	A	G	2: 86,131,264	V133A	probably benign	Het
Olfr385	T	A	11: 73,589,268	T157S	probably benign	Het
Olfr680-ps1	A	T	7: 105,092,771	M16K	probably benign	Het
Olfr701	G	A	7: 106,818,374	C97Y	probably damaging	Het
Pard3	T	A	8: 127,610,582	N1271K	probably damaging	Het
Pcdhb11	A	T	18: 37,421,619	M1L	possibly damaging	Het
Pclo	T	C	5: 14,682,104	V3540A	unknown	Het
Pemt	A	G	11: 59,976,844	F154S	probably damaging	Het
Ptpn18	G	A	1: 34,473,364	D417N	possibly damaging	Het
Rnpc3	G	T	3: 113,613,832	T376K	probably benign	Het
Rpe65	A	G	3: 159,604,609	Y143C	probably damaging	Het
Sbk2	T	C	7: 4,963,149	E12G	probably benign	Het
Slc2a5	T	C	4: 150,129,069	I106T	possibly damaging	Het
Snx13	A	G	12: 35,085,982	D92G	probably damaging	Het
Sorl1	C	T	9: 41,980,688	V1889I	probably benign	Het
Spag17	A	T	3: 99,939,247	N29I	possibly damaging	Het
Speg	T	C	1: 75,401,464	V878A	probably damaging	Het
Timp4	A	G	6: 115,250,460	S53P	probably damaging	Het
Tssk1	G	T	16: 17,895,084	E244D	probably benign	Het
Usp29	A	C	7: 6,961,220	T21P	possibly damaging	Het
Vmn2r85	A	T	10: 130,422,866	V440E	probably benign	Het
Wdr59	T	C	8: 111,490,369	D270G		Het
Zbbx	G	A	3: 75,085,519	P223S	probably damaging	Het
Zfp936	T	A	7: 43,189,815	C235*	probably null	Het

Other mutations in Zranb3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00565:Zranb3	APN	1	128016140	missense	probably benign	0.01
IGL00818:Zranb3	APN	1	128032867	missense	probably damaging	1.00
IGL01360:Zranb3	APN	1	127959885	nonsense	probably null
IGL01704:Zranb3	APN	1	127967939	missense	possibly damaging	0.93
IGL02131:Zranb3	APN	1	127992951	missense	probably damaging	1.00
IGL02466:Zranb3	APN	1	128016092	missense	probably benign	0.08
IGL02825:Zranb3	APN	1	127959752	missense	probably benign	0.13
IGL02836:Zranb3	APN	1	127960825	missense	probably benign	0.00
R0088:Zranb3	UTSW	1	127976462	missense	probably benign
R0279:Zranb3	UTSW	1	127963773	missense	probably benign	0.01
R0423:Zranb3	UTSW	1	128091870	missense	probably damaging	1.00
R0499:Zranb3	UTSW	1	127955080	splice site	probably null
R0562:Zranb3	UTSW	1	128036558	missense	probably benign	0.04
R0972:Zranb3	UTSW	1	127956646	missense	probably damaging	1.00
R1480:Zranb3	UTSW	1	128091862	missense	probably damaging	1.00
R1552:Zranb3	UTSW	1	127960751	splice site	probably benign
R1704:Zranb3	UTSW	1	128092003	start codon destroyed	probably null	0.22
R1817:Zranb3	UTSW	1	128017556	critical splice donor site	probably null
R1818:Zranb3	UTSW	1	128017556	critical splice donor site	probably null
R1819:Zranb3	UTSW	1	128017556	critical splice donor site	probably null
R1951:Zranb3	UTSW	1	127999399	missense	probably damaging	1.00
R1953:Zranb3	UTSW	1	127999399	missense	probably damaging	1.00
R1988:Zranb3	UTSW	1	127959743	missense	probably benign
R2011:Zranb3	UTSW	1	128091901	missense	probably benign	0.00
R3159:Zranb3	UTSW	1	127972949	missense	probably benign
R4179:Zranb3	UTSW	1	127960864	missense	possibly damaging	0.88
R4281:Zranb3	UTSW	1	127963877	missense	possibly damaging	0.69
R4400:Zranb3	UTSW	1	127956655	missense	possibly damaging	0.87
R5236:Zranb3	UTSW	1	128040989	missense	probably damaging	1.00
R5330:Zranb3	UTSW	1	127959720	missense	probably damaging	0.99
R5719:Zranb3	UTSW	1	127963876	missense	probably benign	0.00
R6125:Zranb3	UTSW	1	127959745	missense	probably benign
R6220:Zranb3	UTSW	1	127999404	missense	probably benign	0.44
R6414:Zranb3	UTSW	1	128040957	missense	probably benign	0.08
R6751:Zranb3	UTSW	1	127959819	missense	probably benign
R7229:Zranb3	UTSW	1	128040893	missense	probably benign	0.00
R7419:Zranb3	UTSW	1	127963851	missense	possibly damaging	0.86
R7771:Zranb3	UTSW	1	128032868	missense	probably damaging	1.00
R7980:Zranb3	UTSW	1	128102934	unclassified	probably benign
R8152:Zranb3	UTSW	1	127954995	missense	probably damaging	1.00
R8370:Zranb3	UTSW	1	127967933	missense	probably benign	0.00
R8458:Zranb3	UTSW	1	127992910	missense	probably damaging	1.00
R8816:Zranb3	UTSW	1	128036610	missense	possibly damaging	0.95
R8969:Zranb3	UTSW	1	127960851	missense	possibly damaging	0.80
R9369:Zranb3	UTSW	1	127960091	missense	probably benign	0.00
R9468:Zranb3	UTSW	1	127963759	critical splice donor site	probably null
Z1176:Zranb3	UTSW	1	127965148	missense	possibly damaging	0.55
Z1176:Zranb3	UTSW	1	128036481	missense	probably benign	0.25

Predicted Primers

PCR Primer
(F):5'- ACCTGGCTCATCTCAACCTG -3'
(R):5'- ATCCTTGAAGAATGGATAGTGTGG -3'

Sequencing Primer
(F):5'- CTTCCCAAGTGCTGGCATTGAAG -3'
(R):5'- GTCTTTGAGGAAGAAGCATTTTTAC -3'

Posted On

2019-10-17