Mutagenetix > Incidental Mutations

Incidental Mutation 'R7540:Zfp160'

583865

Institutional Source

Beutler Lab

Gene Symbol

Zfp160

Ensembl Gene

ENSMUSG00000067942

Gene Name

zinc finger protein 160

Synonyms

6720480D16Rik, 6720480D16Rik

MMRRC Submission

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7540 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

21008903-21043070 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 21025660 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 157 (Y157*)

Ref Sequence

ENSEMBL: ENSMUSP00000086191 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088811] [ENSMUST00000231482] [ENSMUST00000232320] [ENSMUST00000232354] [ENSMUST00000232473] [ENSMUST00000232595] [ENSMUST00000232663]

AlphaFold

E9Q459

Predicted Effect

probably null
Transcript: ENSMUST00000088811
AA Change: Y157*

SMART Domains

Protein: ENSMUSP00000086191
Gene: ENSMUSG00000067942
AA Change: Y157*

Domain	Start	End	E-Value	Type
KRAB	8	68	1.91e-29	SMART
low complexity region	100	110	N/A	INTRINSIC
ZnF_C2H2	146	168	1.69e-3	SMART
ZnF_C2H2	174	196	2.91e-2	SMART
ZnF_C2H2	202	224	1.4e-4	SMART
ZnF_C2H2	230	252	3.89e-3	SMART
ZnF_C2H2	258	280	1.72e-4	SMART
ZnF_C2H2	286	308	4.94e-5	SMART
ZnF_C2H2	314	336	2.12e-4	SMART
ZnF_C2H2	342	364	1.12e-3	SMART
ZnF_C2H2	370	392	1.2e-3	SMART
ZnF_C2H2	398	420	6.42e-4	SMART
ZnF_C2H2	426	448	9.08e-4	SMART
ZnF_C2H2	454	476	1.84e-4	SMART
ZnF_C2H2	482	504	1.5e-4	SMART
ZnF_C2H2	510	532	3.44e-4	SMART
ZnF_C2H2	538	560	1.12e-3	SMART
ZnF_C2H2	566	588	2.27e-4	SMART
ZnF_C2H2	594	616	1.04e-3	SMART
ZnF_C2H2	622	644	1.13e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000231482

Predicted Effect

probably benign
Transcript: ENSMUST00000232320

Predicted Effect

probably null
Transcript: ENSMUST00000232354
AA Change: Y157*

Predicted Effect

probably benign
Transcript: ENSMUST00000232473

Predicted Effect

probably benign
Transcript: ENSMUST00000232595

Predicted Effect

probably benign
Transcript: ENSMUST00000232663

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

98% (48/49)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930516K23Rik	T	C	7: 104,059,263	E113G	probably damaging	Het
Adamts8	A	G	9: 30,959,064	E650G	probably damaging	Het
Adgrg7	G	A	16: 56,750,429	T412M	probably damaging	Het
Ank2	C	T	3: 126,988,159	V7I	possibly damaging	Het
Bpifb1	G	A	2: 154,213,111	V308M	probably damaging	Het
C3	A	G	17: 57,206,220	S1476P	probably benign	Het
Dgkb	A	T	12: 37,981,790		probably benign	Het
Fmn1	A	G	2: 113,529,310		probably null	Het
Foxb1	G	A	9: 69,759,859	Q130*	probably null	Het
Gemin5	C	T	11: 58,130,402		probably null	Het
Ghr	T	A	15: 3,319,914	D594V	possibly damaging	Het
Gm10272	A	C	10: 77,706,626	M1L	unknown	Het
Gm11239	C	T	4: 73,668,411		probably benign	Het
Gpr161	G	A	1: 165,318,835	V447M	probably damaging	Het
Hspg2	T	A	4: 137,541,440	S2157T	possibly damaging	Het
Irs1	A	G	1: 82,288,002	V831A	not run	Het
Itga8	T	A	2: 12,111,037	I1014L	possibly damaging	Het
Iws1	T	A	18: 32,080,483	S321R	possibly damaging	Het
Kctd19	G	T	8: 105,386,935	P702Q	probably benign	Het
Man2c1	G	A	9: 57,140,275	D739N	probably damaging	Het
Mboat4	T	A	8: 34,124,024	L205Q	probably damaging	Het
Meis3	C	A	7: 16,177,493	Y38*	probably null	Het
Mroh7	G	T	4: 106,720,398	T361K	possibly damaging	Het
Myl6b	T	A	10: 128,496,280	K106*	probably null	Het
Naaa	T	A	5: 92,263,724	T241S	probably benign	Het
Neurog3	T	G	10: 62,133,977	I172S	probably benign	Het
Nub1	T	G	5: 24,701,529	I351R	probably damaging	Het
Olfr328	C	A	11: 58,551,631	V203L	possibly damaging	Het
Olfr402	A	T	11: 74,155,588	R145W	probably benign	Het
Olfr777	A	T	10: 129,269,134	F63Y	probably benign	Het
Osbp2	T	C	11: 3,717,944	K18E	probably damaging	Het
Pcdh8	A	T	14: 79,771,103	W7R	probably benign	Het
Pcsk7	G	T	9: 45,927,673		probably null	Het
Plin4	G	A	17: 56,104,883	T716I	probably damaging	Het
Prkcb	G	A	7: 122,568,134	V356I	probably damaging	Het
Pus7	G	A	5: 23,760,246	T304I	probably damaging	Het
Rmnd1	A	G	10: 4,403,989	V402A	probably damaging	Het
Slmap	T	C	14: 26,460,191	E329G	probably damaging	Het
Sytl3	T	C	17: 6,681,947		probably benign	Het
Tg	A	G	15: 66,689,927	I1087V	probably benign	Het
Tmco1	A	G	1: 167,326,003	T162A		Het
Tti1	G	A	2: 158,007,996	T441I	probably benign	Het
Tubgcp6	G	T	15: 89,102,323	Q1366K	possibly damaging	Het
Ush1g	T	C	11: 115,318,573	K265R	probably benign	Het
Zdhhc8	A	G	16: 18,227,810	V209A	probably damaging	Het
Zfp423	A	T	8: 87,688,067	C1208S	possibly damaging	Het
Zfyve26	A	T	12: 79,268,676	V1342E	probably damaging	Het
Zmym1	A	T	4: 127,048,757	C613S	probably benign	Het

Other mutations in Zfp160

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00714:Zfp160	APN	17	21026702	missense	probably benign
IGL01019:Zfp160	APN	17	21020826	missense	possibly damaging	0.68
IGL02430:Zfp160	APN	17	21025530	missense	possibly damaging	0.76
R0412:Zfp160	UTSW	17	21026877	missense	probably damaging	0.97
R0600:Zfp160	UTSW	17	21027006	missense	probably benign	0.00
R2146:Zfp160	UTSW	17	21026982	missense	probably benign	0.13
R2157:Zfp160	UTSW	17	21020828	missense	probably benign	0.23
R2411:Zfp160	UTSW	17	21025745	missense	possibly damaging	0.94
R2904:Zfp160	UTSW	17	21025649	missense	probably benign	0.00
R4249:Zfp160	UTSW	17	21025738	missense	probably benign	0.11
R4896:Zfp160	UTSW	17	21020081	missense	probably benign	0.00
R5106:Zfp160	UTSW	17	21026761	missense	probably damaging	0.99
R5342:Zfp160	UTSW	17	21020733	missense	possibly damaging	0.95
R5352:Zfp160	UTSW	17	21026852	missense	probably benign	0.02
R6193:Zfp160	UTSW	17	21026862	missense	probably benign	0.24
R6230:Zfp160	UTSW	17	21026445	missense	probably benign	0.38
R6753:Zfp160	UTSW	17	21020734	missense	probably benign	0.02
R6928:Zfp160	UTSW	17	21041462	missense	probably benign	0.04
R7040:Zfp160	UTSW	17	21026532	missense	probably damaging	1.00
R7255:Zfp160	UTSW	17	21025487	missense	probably benign	0.18
R7497:Zfp160	UTSW	17	21026193	missense	probably benign	0.08
R7510:Zfp160	UTSW	17	21026393	missense	probably benign	0.00
R7627:Zfp160	UTSW	17	21027008	missense	probably damaging	0.99
R8169:Zfp160	UTSW	17	21027036	missense	probably damaging	0.97
R8240:Zfp160	UTSW	17	21026088	missense	probably damaging	0.99
R8330:Zfp160	UTSW	17	21026051	missense	probably damaging	1.00
R8367:Zfp160	UTSW	17	21025542	missense	probably benign	0.22
R8802:Zfp160	UTSW	17	21026605	missense	probably damaging	1.00
R9183:Zfp160	UTSW	17	21020092	missense	possibly damaging	0.84
Z1177:Zfp160	UTSW	17	21026890	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGTAATGAAGCTAAGTCCATCAGC -3'
(R):5'- TCTCACCAGTATGGATTCTTCGATG -3'

Sequencing Primer
(F):5'- AAGTCCATCAGCAGTGGTTC -3'
(R):5'- CGATGACTCTTAAGGTGTGAATTC -3'

Posted On

2019-10-17