Mutagenetix > Incidental Mutations

Incidental Mutation 'R7546:Trcg1'

584220

Institutional Source

Beutler Lab

Gene Symbol

Trcg1

Ensembl Gene

ENSMUSG00000070298

Gene Name

taste receptor cell gene 1

Synonyms

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

R7546 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

57236556-57249864 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 57248338 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 758 (L758P)

Ref Sequence

ENSEMBL: ENSMUSP00000091357 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034846] [ENSMUST00000093837]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000034846

SMART Domains

Protein: ENSMUSP00000034846
Gene: ENSMUSG00000032300

Domain	Start	End	E-Value	Type
low complexity region	265	284	N/A	INTRINSIC
low complexity region	293	299	N/A	INTRINSIC
low complexity region	334	347	N/A	INTRINSIC
low complexity region	649	675	N/A	INTRINSIC
low complexity region	692	728	N/A	INTRINSIC
low complexity region	785	799	N/A	INTRINSIC
low complexity region	933	951	N/A	INTRINSIC
low complexity region	963	977	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000093837
AA Change: L758P

PolyPhen 2 Score 0.337 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000091357
Gene: ENSMUSG00000070298
AA Change: L758P

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
low complexity region	302	335	N/A	INTRINSIC
Blast:SEA	449	549	5e-21	BLAST
low complexity region	580	594	N/A	INTRINSIC

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

100% (40/40)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700122O11Rik	A	G	17: 48,037,521	S36P	probably benign	Het
A530084C06Rik	G	T	13: 31,558,995	R92S	unknown	Het
Ampd1	A	G	3: 103,095,712	T582A	probably benign	Het
Ank1	A	C	8: 23,064,995	N57T	probably damaging	Het
Atxn3	A	G	12: 101,948,002		probably null	Het
Bace2	G	A	16: 97,399,682	A117T	probably benign	Het
Bcl2l15	A	G	3: 103,832,887	N19S	probably benign	Het
Bid	C	A	6: 120,900,151		probably null	Het
Cadps2	A	T	6: 23,626,608	M227K	probably benign	Het
Clip4	G	A	17: 71,828,702	C483Y	possibly damaging	Het
D5Ertd577e	C	T	5: 95,482,680	R139C	probably benign	Het
Dennd4a	A	G	9: 64,873,044	T621A	probably damaging	Het
E2f3	G	A	13: 29,910,129	S383L	probably damaging	Het
F2r	C	T	13: 95,618,350	V9I	probably benign	Het
Gm10424	T	A	5: 95,270,312	H275L	possibly damaging	Het
Gm14226	T	C	2: 155,025,211	S363P	probably damaging	Het
Gm156	T	C	6: 129,772,380	H84R	probably benign	Het
Gstm5	T	C	3: 107,897,294	Y65H	probably damaging	Het
H60c	A	G	10: 3,259,907	W127R	probably damaging	Het
Hist1h2bl	A	G	13: 21,715,870	S92P	probably benign	Het
Itgav	G	T	2: 83,776,550	G448*	probably null	Het
Lhx6	C	T	2: 36,103,345		probably null	Het
Manba	T	A	3: 135,570,246	V816D	probably benign	Het
March10	G	A	11: 105,390,080	P460S	not run	Het
Mmp17	T	A	5: 129,596,589	V244E	probably damaging	Het
Mtrr	C	T	13: 68,582,149		probably benign	Het
Nup160	T	C	2: 90,685,058	I170T	probably damaging	Het
Olfr1255	A	G	2: 89,817,019	N225S	probably benign	Het
Olfr1255	A	T	2: 89,817,194	L283F	probably damaging	Het
Olfr558	T	C	7: 102,709,789	S177P	probably damaging	Het
Pappa	G	A	4: 65,156,115	S302N	possibly damaging	Het
Plekhj1	G	A	10: 80,797,914	A53V	possibly damaging	Het
Prpf8	T	C	11: 75,508,374	V2157A	probably damaging	Het
Rps8	G	A	4: 117,153,907	R200W	probably damaging	Het
Sesn2	A	G	4: 132,499,843	F93L	probably damaging	Het
Slf1	A	G	13: 77,049,192	S768P	probably benign	Het
Stat4	A	G	1: 52,098,463	N471S	probably damaging	Het
Trim35	A	G	14: 66,303,247	T183A	probably benign	Het
Ttc37	C	A	13: 76,134,835	L759M	probably damaging	Het
Ttr	C	A	18: 20,670,045	Y89*	probably null	Het
Zfp90	C	A	8: 106,424,691	H345Q	probably benign	Het
Zmym4	A	G	4: 126,864,168	V1531A	probably damaging	Het

Other mutations in Trcg1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01727:Trcg1	APN	9	57242273	missense	probably benign
IGL01727:Trcg1	APN	9	57242594	missense	probably damaging	0.97
IGL02147:Trcg1	APN	9	57245849	missense	probably benign	0.20
IGL02329:Trcg1	APN	9	57240393	missense	possibly damaging	0.92
IGL02658:Trcg1	APN	9	57242228	nonsense	probably null
IGL02852:Trcg1	APN	9	57241312	missense	possibly damaging	0.94
IGL03163:Trcg1	APN	9	57248347	missense	possibly damaging	0.92
FR4589:Trcg1	UTSW	9	57242202	frame shift	probably null
R0555:Trcg1	UTSW	9	57242333	missense	probably damaging	1.00
R0747:Trcg1	UTSW	9	57241921	missense	probably benign	0.00
R1061:Trcg1	UTSW	9	57245873	missense	possibly damaging	0.66
R1521:Trcg1	UTSW	9	57242465	missense	probably benign	0.36
R1622:Trcg1	UTSW	9	57248672	missense	possibly damaging	0.94
R1652:Trcg1	UTSW	9	57245573	missense	probably damaging	0.99
R4677:Trcg1	UTSW	9	57245861	missense	possibly damaging	0.94
R4879:Trcg1	UTSW	9	57246720	missense	probably damaging	0.99
R5013:Trcg1	UTSW	9	57242279	missense	probably damaging	0.99
R5141:Trcg1	UTSW	9	57241304	missense	probably damaging	1.00
R5690:Trcg1	UTSW	9	57241811	missense	probably benign	0.36
R6416:Trcg1	UTSW	9	57241330	missense	possibly damaging	0.46
R6980:Trcg1	UTSW	9	57245573	missense	probably damaging	0.99
R7022:Trcg1	UTSW	9	57241569	missense	possibly damaging	0.46
R7172:Trcg1	UTSW	9	57248335	missense	probably benign	0.01
R7276:Trcg1	UTSW	9	57242579	missense	probably damaging	0.99
R7412:Trcg1	UTSW	9	57241483	missense	probably benign	0.00
R7942:Trcg1	UTSW	9	57242216	missense	probably benign
R8087:Trcg1	UTSW	9	57248674	missense	probably damaging	0.99
R8094:Trcg1	UTSW	9	57242281	missense	probably benign	0.01
R8825:Trcg1	UTSW	9	57241471	missense	probably benign	0.19

Predicted Primers

PCR Primer
(F):5'- AAGAACTTTGGTCAAGTGGTGG -3'
(R):5'- ACTTGGAGCCAATGTACCCTG -3'

Sequencing Primer
(F):5'- AATTCCAGTGAGTGTTCGGTTCAC -3'
(R):5'- CCTGGGGGCTCATAGGGAAG -3'

Posted On

2019-10-17