Mutagenetix > Incidental Mutation

Incidental Mutation 'R7552:Fras1'

584460

Institutional Source

Beutler Lab

Gene Symbol

Fras1

Ensembl Gene

ENSMUSG00000034687

Gene Name

Fraser extracellular matrix complex subunit 1

Synonyms

bl, E130113P14Rik

MMRRC Submission

045621-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7552 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

96373955-96784728 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 96768438 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Asparagine at position 3444 (D3444N)

Ref Sequence

ENSEMBL: ENSMUSP00000043250 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000036019]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000036019
AA Change: D3444N

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000043250
Gene: ENSMUSG00000034687
AA Change: D3444N

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
VWC	27	86	9.23e-9	SMART
VWC	94	151	9.37e-10	SMART
VWC	158	215	8.61e-9	SMART
VWC	220	277	7.1e-10	SMART
VWC	284	341	7.8e-7	SMART
VWC	365	415	1.93e-1	SMART
FU	408	459	3.33e-1	SMART
FU	461	504	9.12e-8	SMART
EGF_like	466	495	6.67e1	SMART
FU	506	552	6.01e-8	SMART
FU	554	598	2.21e-6	SMART
FU	601	646	1.28e-11	SMART
FU	648	704	4.19e-7	SMART
FU	707	752	9.12e-8	SMART
FU	754	799	1.11e-6	SMART
EGF_like	759	790	7.23e1	SMART
FU	802	851	5.44e-6	SMART
EGF_like	807	842	4.55e1	SMART
FU	853	899	7.4e-8	SMART
FU	902	947	4.78e-2	SMART
FU	951	996	4.52e-12	SMART
EGF_like	956	987	2.75e1	SMART
FU	998	1041	1.38e-7	SMART
FU	1045	1088	9.7e-3	SMART
EGF_like	1057	1096	3.16e1	SMART
Pfam:Cadherin_3	1098	1198	5.2e-12	PFAM
Pfam:Cadherin_3	1167	1309	6.5e-27	PFAM
Pfam:Cadherin_3	1278	1442	7e-24	PFAM
Pfam:Cadherin_3	1411	1560	1.3e-23	PFAM
Pfam:Cadherin_3	1561	1693	6.4e-15	PFAM
Pfam:Cadherin_3	1695	1814	1.1e-10	PFAM
Pfam:Cadherin_3	1780	1940	1.6e-18	PFAM
Pfam:Cadherin_3	1906	2061	2.8e-22	PFAM
Pfam:Cadherin_3	2063	2181	3.3e-18	PFAM
Pfam:Cadherin_3	2172	2295	1.4e-26	PFAM
Pfam:Cadherin_3	2296	2408	2.3e-31	PFAM
Pfam:Cadherin_3	2413	2540	7.9e-23	PFAM
Calx_beta	2544	2648	1.23e-10	SMART
Calx_beta	2661	2772	3.3e-11	SMART
Calx_beta	2787	2892	1.21e-9	SMART
Calx_beta	2907	3009	4.45e-3	SMART
Calx_beta	3027	3131	1.5e-14	SMART
Blast:Calx_beta	3162	3187	1e-5	BLAST
transmembrane domain	3902	3924	N/A	INTRINSIC
low complexity region	3927	3935	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (89/89)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an extracellular matrix protein that appears to function in the regulation of epidermal-basement membrane adhesion and organogenesis during development. Mutations in this gene cause Fraser syndrome, a multisystem malformation that can include craniofacial, urogenital and respiratory system abnormalities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
PHENOTYPE: Mice homozygous for mutations at this locus display a significant amount of embryonic lethality due to hemorrhaging of embryonic blisters. Survival is variable on genetic backgrounds. Kidney development is severely affected and syndactyly is common. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 87 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd17a	A	G	10: 80,583,903 (GRCm38)	Y290H	probably benign	Het
Aco2	T	C	15: 81,903,941 (GRCm38)	V257A	probably damaging	Het
Adam26a	C	A	8: 43,569,970 (GRCm38)	C161F	possibly damaging	Het
Adam9	G	A	8: 24,955,972 (GRCm38)	P820S	unknown	Het
Adcy4	G	A	14: 55,773,465 (GRCm38)	T665M	probably benign	Het
Ahnak	T	A	19: 9,006,824 (GRCm38)	M1824K	probably benign	Het
Alpi	T	C	1: 87,099,073 (GRCm38)	N428D	probably benign	Het
Ano5	C	T	7: 51,546,780 (GRCm38)	P153L	probably benign	Het
Atp1a1	A	T	3: 101,582,121 (GRCm38)	L725*	probably null	Het
Atp8b2	G	A	3: 89,946,764 (GRCm38)	T595I	probably damaging	Het
Ccdc66	T	C	14: 27,498,863 (GRCm38)	I35V	possibly damaging	Het
Cd163	A	G	6: 124,307,228 (GRCm38)	T120A	probably benign	Het
Cmah	A	T	13: 24,456,955 (GRCm38)	T396S	possibly damaging	Het
Cmya5	C	T	13: 93,069,312 (GRCm38)	V3350I	probably benign	Het
Commd9	A	C	2: 101,901,065 (GRCm38)	K198N	probably damaging	Het
Cxcr1	A	G	1: 74,192,614 (GRCm38)	V83A	probably benign	Het
D630045J12Rik	A	G	6: 38,148,448 (GRCm38)	S1544P	probably damaging	Het
Ddt	A	T	10: 75,773,214 (GRCm38)		probably null	Het
Depdc7	A	G	2: 104,727,240 (GRCm38)	S222P	possibly damaging	Het
Ehd2	A	G	7: 15,950,506 (GRCm38)	I456T	probably damaging	Het
Epas1	A	T	17: 86,829,043 (GRCm38)	M748L	probably benign	Het
Esr1	G	A	10: 4,856,903 (GRCm38)	R273H	probably damaging	Het
Hand2	A	G	8: 57,322,237 (GRCm38)	R111G	probably damaging	Het
Hecw1	A	T	13: 14,316,250 (GRCm38)	V306E	probably damaging	Het
Ift140	T	C	17: 25,033,115 (GRCm38)	I312T	probably benign	Het
Ikbke	A	T	1: 131,272,150 (GRCm38)	L257*	probably null	Het
Kcnk5	G	A	14: 20,142,281 (GRCm38)	P271S	probably benign	Het
Lama1	T	C	17: 67,737,667 (GRCm38)	V187A		Het
Lemd2	A	T	17: 27,193,836 (GRCm38)		probably null	Het
Lig3	T	A	11: 82,788,891 (GRCm38)	D341E	probably benign	Het
Lpcat1	C	T	13: 73,494,895 (GRCm38)	S196L	probably damaging	Het
Lrp1b	A	T	2: 40,677,570 (GRCm38)	D4048E		Het
Lrrfip1	G	A	1: 91,105,283 (GRCm38)	E158K	probably damaging	Het
Mcm5	T	C	8: 75,121,592 (GRCm38)	S490P	probably damaging	Het
Mia3	A	C	1: 183,365,695 (GRCm38)	Y73*	probably null	Het
Minar1	T	A	9: 89,601,835 (GRCm38)	H503L	probably benign	Het
Msr1	A	T	8: 39,623,962 (GRCm38)	N202K	probably benign	Het
N4bp2l2	A	T	5: 150,661,821 (GRCm38)	Y231*	probably null	Het
Nbeal2	A	G	9: 110,653,917 (GRCm38)	W11R	probably benign	Het
Ncor1	T	C	11: 62,373,424 (GRCm38)	E384G	possibly damaging	Het
Neb	A	G	2: 52,247,190 (GRCm38)	V254A		Het
Nlrp9b	T	C	7: 20,045,766 (GRCm38)	S785P	probably benign	Het
Olfr1191-ps1	A	G	2: 88,643,408 (GRCm38)	I214V	probably benign	Het
Oprd1	T	G	4: 132,113,781 (GRCm38)	I289L	possibly damaging	Het
Or4c10	G	T	2: 89,930,720 (GRCm38)	D304Y	probably benign	Het
Or5m12	A	G	2: 85,904,103 (GRCm38)	V317A	probably benign	Het
Or6a2	T	C	7: 107,001,327 (GRCm38)	T178A	probably benign	Het
Or6c2	T	A	10: 129,526,560 (GRCm38)	F111Y	possibly damaging	Het
Orc1	A	G	4: 108,588,754 (GRCm38)	N23S	probably benign	Het
Paip1	A	T	13: 119,440,820 (GRCm38)	H149L	possibly damaging	Het
Pdhx	A	T	2: 103,046,754 (GRCm38)	D103E	probably benign	Het
Pi4ka	T	C	16: 17,291,216 (GRCm38)	Y1614C		Het
Pias3	ACC	AC	3: 96,701,385 (GRCm38)		probably null	Het
Pigb	A	G	9: 73,034,488 (GRCm38)	V163A	probably benign	Het
Pla2g4d	A	G	2: 120,284,139 (GRCm38)	I37T	possibly damaging	Het
Ppfia1	A	T	7: 144,506,245 (GRCm38)	V610D	probably damaging	Het
Pramel41	G	A	5: 94,448,535 (GRCm38)	C477Y	probably damaging	Het
Prss1	T	C	6: 41,462,573 (GRCm38)	V80A	probably benign	Het
Psph	G	A	5: 129,770,736 (GRCm38)	R49W	probably benign	Het
Qrich2	T	C	11: 116,456,254 (GRCm38)	Y1248C	possibly damaging	Het
Rlbp1	T	A	7: 79,380,113 (GRCm38)	Y124F	probably damaging	Het
Scn4a	T	A	11: 106,349,169 (GRCm38)	E74V	probably benign	Het
Sdk2	A	T	11: 113,873,213 (GRCm38)	I249N	possibly damaging	Het
Sgsh	A	T	11: 119,346,552 (GRCm38)	L412Q	probably damaging	Het
Shank1	C	A	7: 44,353,028 (GRCm38)	D1390E	probably benign	Het
Slc12a6	A	G	2: 112,341,974 (GRCm38)	D421G	probably damaging	Het
Snx29	T	A	16: 11,420,785 (GRCm38)		probably null	Het
Spata31d1c	A	G	13: 65,036,123 (GRCm38)	Y493C	probably damaging	Het
Stmn4	G	A	14: 66,356,278 (GRCm38)	G40E	probably damaging	Het
Stox2	A	G	8: 47,203,119 (GRCm38)		probably null	Het
Strada	T	C	11: 106,187,004 (GRCm38)	S45G	unknown	Het
Taco1	G	A	11: 106,071,948 (GRCm38)	G154S	probably benign	Het
Tas2r121	T	C	6: 132,700,542 (GRCm38)	M156V	probably benign	Het
Tbc1d31	A	G	15: 57,940,740 (GRCm38)	S384G	probably benign	Het
Tbc1d9	A	T	8: 83,239,931 (GRCm38)	D387V	probably damaging	Het
Tet3	A	G	6: 83,368,307 (GRCm38)	L1716P	probably damaging	Het
Thbs1	A	G	2: 118,113,362 (GRCm38)	K154E	possibly damaging	Het
Ush2a	T	C	1: 188,267,044 (GRCm38)	Y184H	possibly damaging	Het
Vmn1r235	C	A	17: 21,261,451 (GRCm38)	Q13K	probably benign	Het
Vmn2r15	A	T	5: 109,292,908 (GRCm38)	Y361*	probably null	Het
Vmn2r88	A	T	14: 51,410,858 (GRCm38)		probably null	Het
Vps54	T	C	11: 21,298,831 (GRCm38)	V460A	probably benign	Het
Wdr64	A	G	1: 175,785,581 (GRCm38)	N674S	possibly damaging	Het
Zfp592	A	G	7: 81,023,642 (GRCm38)	D118G	probably benign	Het
Zfp763	A	G	17: 33,018,651 (GRCm38)	Y507H	probably benign	Het
Zfp934	T	C	13: 62,492,891 (GRCm38)	E16G	probably damaging	Het
Zfyve26	G	A	12: 79,290,957 (GRCm38)	L94F	probably damaging	Het

Other mutations in Fras1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Fras1	APN	5	96,739,358 (GRCm38)	missense	possibly damaging	0.55
IGL00507:Fras1	APN	5	96,778,189 (GRCm38)	missense	probably damaging	1.00
IGL00672:Fras1	APN	5	96,759,450 (GRCm38)	splice site	probably benign
IGL00772:Fras1	APN	5	96,636,112 (GRCm38)	missense	probably benign	0.42
IGL00844:Fras1	APN	5	96,534,853 (GRCm38)	splice site	probably benign
IGL00913:Fras1	APN	5	96,695,076 (GRCm38)	missense	probably damaging	0.99
IGL00959:Fras1	APN	5	96,781,281 (GRCm38)	missense	probably damaging	0.96
IGL00966:Fras1	APN	5	96,555,221 (GRCm38)	missense	probably benign	0.00
IGL01296:Fras1	APN	5	96,673,698 (GRCm38)	missense	probably null	0.58
IGL01307:Fras1	APN	5	96,781,692 (GRCm38)	missense	probably benign
IGL01481:Fras1	APN	5	96,657,241 (GRCm38)	missense	probably damaging	1.00
IGL01525:Fras1	APN	5	96,739,336 (GRCm38)	missense	probably damaging	0.99
IGL01599:Fras1	APN	5	96,709,891 (GRCm38)	missense	possibly damaging	0.94
IGL01646:Fras1	APN	5	96,758,148 (GRCm38)	missense	probably benign	0.29
IGL01795:Fras1	APN	5	96,778,045 (GRCm38)	missense	probably damaging	1.00
IGL01867:Fras1	APN	5	96,588,131 (GRCm38)	missense	probably benign
IGL01869:Fras1	APN	5	96,708,783 (GRCm38)	splice site	probably benign
IGL01923:Fras1	APN	5	96,735,280 (GRCm38)	missense	probably damaging	1.00
IGL01982:Fras1	APN	5	96,739,248 (GRCm38)	missense	possibly damaging	0.46
IGL02109:Fras1	APN	5	96,700,523 (GRCm38)	missense	probably benign
IGL02132:Fras1	APN	5	96,781,637 (GRCm38)	nonsense	probably null
IGL02171:Fras1	APN	5	96,735,181 (GRCm38)	missense	probably benign	0.15
IGL02213:Fras1	APN	5	96,645,871 (GRCm38)	nonsense	probably null
IGL02277:Fras1	APN	5	96,588,118 (GRCm38)	missense	probably benign	0.00
IGL02507:Fras1	APN	5	96,657,408 (GRCm38)	missense	possibly damaging	0.95
IGL02589:Fras1	APN	5	96,769,513 (GRCm38)	missense	probably damaging	1.00
IGL02671:Fras1	APN	5	96,728,616 (GRCm38)	missense	possibly damaging	0.91
IGL02677:Fras1	APN	5	96,545,024 (GRCm38)	missense	probably damaging	1.00
IGL02691:Fras1	APN	5	96,744,705 (GRCm38)	missense	possibly damaging	0.68
IGL02741:Fras1	APN	5	96,691,371 (GRCm38)	missense	probably benign	0.35
IGL02836:Fras1	APN	5	96,534,866 (GRCm38)	missense	possibly damaging	0.67
IGL02850:Fras1	APN	5	96,778,175 (GRCm38)	missense	probably damaging	1.00
IGL02998:Fras1	APN	5	96,702,181 (GRCm38)	missense	possibly damaging	0.82
IGL03040:Fras1	APN	5	96,710,101 (GRCm38)	missense	probably benign
IGL03078:Fras1	APN	5	96,636,135 (GRCm38)	missense	probably damaging	1.00
IGL03096:Fras1	APN	5	96,764,901 (GRCm38)	missense	probably damaging	1.00
IGL03102:Fras1	APN	5	96,726,535 (GRCm38)	missense	probably benign	0.11
IGL03183:Fras1	APN	5	96,733,781 (GRCm38)	splice site	probably benign
IGL03189:Fras1	APN	5	96,743,071 (GRCm38)	missense	probably benign	0.00
IGL03193:Fras1	APN	5	96,778,106 (GRCm38)	missense	probably damaging	0.99
IGL03292:Fras1	APN	5	96,707,491 (GRCm38)	missense	probably damaging	1.00
IGL03328:Fras1	APN	5	96,781,760 (GRCm38)	missense	probably damaging	0.96
IGL03335:Fras1	APN	5	96,733,944 (GRCm38)	splice site	probably benign
IGL03394:Fras1	APN	5	96,667,477 (GRCm38)	missense	probably damaging	0.98
IGL03404:Fras1	APN	5	96,728,581 (GRCm38)	missense	probably damaging	0.99
baby_ruth	UTSW	5	96,708,758 (GRCm38)	missense	probably benign	0.01
BB002:Fras1	UTSW	5	96,781,584 (GRCm38)	missense	probably damaging	0.96
BB012:Fras1	UTSW	5	96,781,584 (GRCm38)	missense	probably damaging	0.96
G1patch:Fras1	UTSW	5	96,781,340 (GRCm38)	missense	possibly damaging	0.91
I0000:Fras1	UTSW	5	96,740,829 (GRCm38)	missense	probably damaging	0.99
PIT4581001:Fras1	UTSW	5	96,555,301 (GRCm38)	missense	probably benign	0.01
R0028:Fras1	UTSW	5	96,677,316 (GRCm38)	missense	probably benign	0.07
R0049:Fras1	UTSW	5	96,776,622 (GRCm38)	missense	probably benign	0.07
R0049:Fras1	UTSW	5	96,776,622 (GRCm38)	missense	probably benign	0.07
R0099:Fras1	UTSW	5	96,614,917 (GRCm38)	critical splice donor site	probably null
R0109:Fras1	UTSW	5	96,710,077 (GRCm38)	missense	probably benign	0.01
R0158:Fras1	UTSW	5	96,776,634 (GRCm38)	missense	possibly damaging	0.83
R0268:Fras1	UTSW	5	96,737,009 (GRCm38)	missense	probably damaging	0.99
R0305:Fras1	UTSW	5	96,596,888 (GRCm38)	missense	probably benign
R0352:Fras1	UTSW	5	96,726,540 (GRCm38)	missense	probably damaging	0.97
R0359:Fras1	UTSW	5	96,762,590 (GRCm38)	missense	probably damaging	0.98
R0371:Fras1	UTSW	5	96,555,331 (GRCm38)	missense	possibly damaging	0.90
R0379:Fras1	UTSW	5	96,755,509 (GRCm38)	nonsense	probably null
R0395:Fras1	UTSW	5	96,769,653 (GRCm38)	missense	possibly damaging	0.50
R0417:Fras1	UTSW	5	96,691,372 (GRCm38)	missense	probably benign	0.18
R0454:Fras1	UTSW	5	96,762,665 (GRCm38)	missense	probably damaging	0.96
R0456:Fras1	UTSW	5	96,714,343 (GRCm38)	splice site	probably null
R0456:Fras1	UTSW	5	96,554,788 (GRCm38)	missense	probably damaging	1.00
R0464:Fras1	UTSW	5	96,636,803 (GRCm38)	missense	probably damaging	0.98
R0613:Fras1	UTSW	5	96,700,488 (GRCm38)	splice site	probably benign
R0652:Fras1	UTSW	5	96,781,340 (GRCm38)	missense	possibly damaging	0.91
R0675:Fras1	UTSW	5	96,667,387 (GRCm38)	splice site	probably benign
R0765:Fras1	UTSW	5	96,552,796 (GRCm38)	missense	probably benign	0.00
R0783:Fras1	UTSW	5	96,768,430 (GRCm38)	missense	probably damaging	1.00
R0811:Fras1	UTSW	5	96,752,998 (GRCm38)	missense	probably benign	0.35
R0812:Fras1	UTSW	5	96,752,998 (GRCm38)	missense	probably benign	0.35
R0943:Fras1	UTSW	5	96,726,543 (GRCm38)	missense	probably benign	0.00
R1037:Fras1	UTSW	5	96,714,463 (GRCm38)	missense	probably damaging	0.97
R1104:Fras1	UTSW	5	96,708,671 (GRCm38)	missense	probably benign	0.00
R1108:Fras1	UTSW	5	96,642,629 (GRCm38)	missense	probably damaging	0.99
R1332:Fras1	UTSW	5	96,707,308 (GRCm38)	missense	probably benign	0.00
R1336:Fras1	UTSW	5	96,707,308 (GRCm38)	missense	probably benign	0.00
R1458:Fras1	UTSW	5	96,600,733 (GRCm38)	missense	probably benign	0.00
R1495:Fras1	UTSW	5	96,528,586 (GRCm38)	missense	possibly damaging	0.49
R1499:Fras1	UTSW	5	96,743,187 (GRCm38)	missense	probably benign	0.31
R1528:Fras1	UTSW	5	96,636,819 (GRCm38)	missense	probably damaging	0.99
R1532:Fras1	UTSW	5	96,713,996 (GRCm38)	missense	probably damaging	1.00
R1556:Fras1	UTSW	5	96,743,062 (GRCm38)	missense	possibly damaging	0.88
R1625:Fras1	UTSW	5	96,709,978 (GRCm38)	missense	possibly damaging	0.94
R1625:Fras1	UTSW	5	96,713,990 (GRCm38)	missense	probably damaging	1.00
R1645:Fras1	UTSW	5	96,700,586 (GRCm38)	missense	possibly damaging	0.90
R1647:Fras1	UTSW	5	96,726,613 (GRCm38)	critical splice donor site	probably null
R1648:Fras1	UTSW	5	96,726,613 (GRCm38)	critical splice donor site	probably null
R1661:Fras1	UTSW	5	96,598,909 (GRCm38)	missense	probably damaging	1.00
R1665:Fras1	UTSW	5	96,598,909 (GRCm38)	missense	probably damaging	1.00
R1682:Fras1	UTSW	5	96,645,873 (GRCm38)	missense	probably benign	0.00
R1701:Fras1	UTSW	5	96,600,784 (GRCm38)	missense	probably benign	0.00
R1716:Fras1	UTSW	5	96,552,725 (GRCm38)	missense	probably benign	0.10
R1718:Fras1	UTSW	5	96,554,889 (GRCm38)	splice site	probably null
R1800:Fras1	UTSW	5	96,709,882 (GRCm38)	missense	probably benign
R1806:Fras1	UTSW	5	96,764,976 (GRCm38)	missense	possibly damaging	0.88
R1806:Fras1	UTSW	5	96,713,970 (GRCm38)	splice site	probably benign
R1822:Fras1	UTSW	5	96,770,688 (GRCm38)	missense	probably damaging	1.00
R1823:Fras1	UTSW	5	96,770,688 (GRCm38)	missense	probably damaging	1.00
R1824:Fras1	UTSW	5	96,770,688 (GRCm38)	missense	probably damaging	1.00
R1847:Fras1	UTSW	5	96,749,423 (GRCm38)	splice site	probably null
R1929:Fras1	UTSW	5	96,667,437 (GRCm38)	missense	probably benign	0.24
R1951:Fras1	UTSW	5	96,712,383 (GRCm38)	missense	probably benign	0.38
R2093:Fras1	UTSW	5	96,781,203 (GRCm38)	missense	probably damaging	1.00
R2283:Fras1	UTSW	5	96,654,305 (GRCm38)	missense	probably benign	0.10
R2884:Fras1	UTSW	5	96,700,268 (GRCm38)	missense	probably benign	0.07
R2913:Fras1	UTSW	5	96,733,915 (GRCm38)	missense	probably benign
R2914:Fras1	UTSW	5	96,733,915 (GRCm38)	missense	probably benign
R3054:Fras1	UTSW	5	96,764,943 (GRCm38)	missense	probably damaging	0.99
R3117:Fras1	UTSW	5	96,771,712 (GRCm38)	missense	probably damaging	1.00
R3118:Fras1	UTSW	5	96,771,712 (GRCm38)	missense	probably damaging	1.00
R3691:Fras1	UTSW	5	96,781,512 (GRCm38)	missense	probably benign	0.02
R3714:Fras1	UTSW	5	96,645,970 (GRCm38)	critical splice donor site	probably null
R3715:Fras1	UTSW	5	96,645,970 (GRCm38)	critical splice donor site	probably null
R3801:Fras1	UTSW	5	96,733,932 (GRCm38)	missense	probably benign	0.26
R3961:Fras1	UTSW	5	96,677,385 (GRCm38)	critical splice donor site	probably null
R4065:Fras1	UTSW	5	96,770,683 (GRCm38)	missense	possibly damaging	0.64
R4066:Fras1	UTSW	5	96,770,683 (GRCm38)	missense	possibly damaging	0.64
R4076:Fras1	UTSW	5	96,743,158 (GRCm38)	missense	probably damaging	1.00
R4124:Fras1	UTSW	5	96,770,653 (GRCm38)	missense	probably benign	0.05
R4127:Fras1	UTSW	5	96,770,653 (GRCm38)	missense	probably benign	0.05
R4153:Fras1	UTSW	5	96,776,735 (GRCm38)	missense	probably benign	0.17
R4233:Fras1	UTSW	5	96,714,376 (GRCm38)	missense	possibly damaging	0.91
R4273:Fras1	UTSW	5	96,614,904 (GRCm38)	missense	probably benign	0.00
R4355:Fras1	UTSW	5	96,700,242 (GRCm38)	missense	probably benign
R4401:Fras1	UTSW	5	96,642,620 (GRCm38)	missense	probably damaging	0.97
R4402:Fras1	UTSW	5	96,642,620 (GRCm38)	missense	probably damaging	0.97
R4403:Fras1	UTSW	5	96,642,620 (GRCm38)	missense	probably damaging	0.97
R4505:Fras1	UTSW	5	96,781,348 (GRCm38)	missense	probably damaging	1.00
R4548:Fras1	UTSW	5	96,709,895 (GRCm38)	missense	probably benign	0.00
R4559:Fras1	UTSW	5	96,781,289 (GRCm38)	missense	probably damaging	1.00
R4629:Fras1	UTSW	5	96,776,734 (GRCm38)	missense	probably benign	0.00
R4637:Fras1	UTSW	5	96,778,088 (GRCm38)	missense	probably damaging	1.00
R4678:Fras1	UTSW	5	96,700,568 (GRCm38)	missense	probably benign	0.13
R4707:Fras1	UTSW	5	96,735,238 (GRCm38)	missense	probably damaging	0.96
R4735:Fras1	UTSW	5	96,588,163 (GRCm38)	missense	probably benign	0.00
R4756:Fras1	UTSW	5	96,781,659 (GRCm38)	missense	probably benign	0.00
R4762:Fras1	UTSW	5	96,731,618 (GRCm38)	missense	probably benign
R4820:Fras1	UTSW	5	96,728,653 (GRCm38)	missense	probably benign	0.00
R4847:Fras1	UTSW	5	96,544,992 (GRCm38)	missense	possibly damaging	0.94
R4857:Fras1	UTSW	5	96,778,159 (GRCm38)	missense	probably benign	0.00
R4909:Fras1	UTSW	5	96,708,758 (GRCm38)	missense	probably benign	0.01
R4931:Fras1	UTSW	5	96,636,840 (GRCm38)	missense	probably benign	0.02
R4938:Fras1	UTSW	5	96,776,724 (GRCm38)	missense	probably damaging	0.99
R4952:Fras1	UTSW	5	96,647,498 (GRCm38)	missense	probably benign	0.01
R4965:Fras1	UTSW	5	96,726,580 (GRCm38)	missense	possibly damaging	0.95
R4989:Fras1	UTSW	5	96,650,682 (GRCm38)	missense	possibly damaging	0.75
R5151:Fras1	UTSW	5	96,645,110 (GRCm38)	missense	probably damaging	1.00
R5168:Fras1	UTSW	5	96,708,757 (GRCm38)	missense	probably benign	0.00
R5182:Fras1	UTSW	5	96,636,173 (GRCm38)	nonsense	probably null
R5214:Fras1	UTSW	5	96,769,593 (GRCm38)	missense	probably damaging	1.00
R5220:Fras1	UTSW	5	96,768,363 (GRCm38)	missense	probably damaging	1.00
R5235:Fras1	UTSW	5	96,600,750 (GRCm38)	missense	probably benign	0.02
R5242:Fras1	UTSW	5	96,657,250 (GRCm38)	missense	probably benign	0.11
R5253:Fras1	UTSW	5	96,741,025 (GRCm38)	missense	probably damaging	0.99
R5260:Fras1	UTSW	5	96,735,187 (GRCm38)	missense	possibly damaging	0.79
R5301:Fras1	UTSW	5	96,657,266 (GRCm38)	missense	possibly damaging	0.88
R5411:Fras1	UTSW	5	96,645,160 (GRCm38)	missense	probably benign	0.00
R5467:Fras1	UTSW	5	96,780,053 (GRCm38)	missense	probably benign	0.04
R5543:Fras1	UTSW	5	96,528,535 (GRCm38)	missense	probably benign	0.01
R5555:Fras1	UTSW	5	96,677,377 (GRCm38)	missense	probably benign	0.34
R5602:Fras1	UTSW	5	96,737,021 (GRCm38)	missense	probably damaging	1.00
R5664:Fras1	UTSW	5	96,728,535 (GRCm38)	missense	possibly damaging	0.91
R5695:Fras1	UTSW	5	96,781,344 (GRCm38)	missense	probably damaging	1.00
R5717:Fras1	UTSW	5	96,781,737 (GRCm38)	missense	possibly damaging	0.93
R5742:Fras1	UTSW	5	96,768,381 (GRCm38)	missense	possibly damaging	0.50
R5759:Fras1	UTSW	5	96,709,916 (GRCm38)	missense	probably benign	0.02
R5766:Fras1	UTSW	5	96,731,689 (GRCm38)	missense	possibly damaging	0.91
R5890:Fras1	UTSW	5	96,645,948 (GRCm38)	missense	probably benign
R6052:Fras1	UTSW	5	96,764,866 (GRCm38)	missense	probably damaging	1.00
R6058:Fras1	UTSW	5	96,709,985 (GRCm38)	missense	probably benign
R6256:Fras1	UTSW	5	96,733,843 (GRCm38)	missense	possibly damaging	0.84
R6306:Fras1	UTSW	5	96,764,946 (GRCm38)	missense	probably damaging	1.00
R6494:Fras1	UTSW	5	96,759,564 (GRCm38)	missense	possibly damaging	0.59
R6638:Fras1	UTSW	5	96,758,094 (GRCm38)	missense	possibly damaging	0.94
R6647:Fras1	UTSW	5	96,735,202 (GRCm38)	missense	probably damaging	1.00
R6725:Fras1	UTSW	5	96,781,340 (GRCm38)	missense	possibly damaging	0.91
R6769:Fras1	UTSW	5	96,598,941 (GRCm38)	missense	possibly damaging	0.60
R6771:Fras1	UTSW	5	96,598,941 (GRCm38)	missense	possibly damaging	0.60
R6837:Fras1	UTSW	5	96,726,973 (GRCm38)	missense	probably damaging	0.99
R6841:Fras1	UTSW	5	96,728,551 (GRCm38)	missense	probably damaging	0.99
R6863:Fras1	UTSW	5	96,543,306 (GRCm38)	missense	probably benign	0.19
R6868:Fras1	UTSW	5	96,682,378 (GRCm38)	missense	probably benign	0.38
R6936:Fras1	UTSW	5	96,768,352 (GRCm38)	missense	possibly damaging	0.92
R6997:Fras1	UTSW	5	96,614,873 (GRCm38)	nonsense	probably null
R7023:Fras1	UTSW	5	96,710,084 (GRCm38)	missense	probably benign	0.00
R7091:Fras1	UTSW	5	96,708,676 (GRCm38)	missense	probably benign
R7102:Fras1	UTSW	5	96,571,041 (GRCm38)	missense	probably benign
R7120:Fras1	UTSW	5	96,752,960 (GRCm38)	nonsense	probably null
R7124:Fras1	UTSW	5	96,714,401 (GRCm38)	missense	probably damaging	1.00
R7129:Fras1	UTSW	5	96,781,284 (GRCm38)	missense	probably benign	0.00
R7173:Fras1	UTSW	5	96,778,078 (GRCm38)	missense	probably damaging	1.00
R7174:Fras1	UTSW	5	96,755,577 (GRCm38)	critical splice donor site	probably null
R7185:Fras1	UTSW	5	96,636,776 (GRCm38)	missense	probably damaging	1.00
R7191:Fras1	UTSW	5	96,614,912 (GRCm38)	missense	probably benign	0.05
R7216:Fras1	UTSW	5	96,739,314 (GRCm38)	missense	probably damaging	1.00
R7222:Fras1	UTSW	5	96,636,809 (GRCm38)	missense	probably benign	0.00
R7222:Fras1	UTSW	5	96,636,186 (GRCm38)	missense	probably damaging	1.00
R7320:Fras1	UTSW	5	96,709,886 (GRCm38)	missense	probably benign	0.03
R7335:Fras1	UTSW	5	96,736,970 (GRCm38)	missense	possibly damaging	0.82
R7378:Fras1	UTSW	5	96,596,785 (GRCm38)	missense	probably damaging	0.98
R7394:Fras1	UTSW	5	96,712,450 (GRCm38)	nonsense	probably null
R7412:Fras1	UTSW	5	96,614,889 (GRCm38)	missense	probably benign	0.06
R7422:Fras1	UTSW	5	96,673,599 (GRCm38)	missense	probably benign	0.21
R7559:Fras1	UTSW	5	96,740,854 (GRCm38)	missense	possibly damaging	0.82
R7575:Fras1	UTSW	5	96,543,314 (GRCm38)	missense	probably benign	0.02
R7578:Fras1	UTSW	5	96,684,437 (GRCm38)	missense	probably damaging	1.00
R7600:Fras1	UTSW	5	96,684,436 (GRCm38)	missense	probably damaging	1.00
R7669:Fras1	UTSW	5	96,692,624 (GRCm38)	missense	probably benign	0.01
R7710:Fras1	UTSW	5	96,645,103 (GRCm38)	nonsense	probably null
R7722:Fras1	UTSW	5	96,769,554 (GRCm38)	missense	probably damaging	1.00
R7726:Fras1	UTSW	5	96,712,451 (GRCm38)	missense	probably benign	0.41
R7745:Fras1	UTSW	5	96,726,895 (GRCm38)	missense	probably benign	0.11
R7777:Fras1	UTSW	5	96,752,904 (GRCm38)	missense	probably damaging	1.00
R7923:Fras1	UTSW	5	96,739,318 (GRCm38)	missense	probably damaging	1.00
R7925:Fras1	UTSW	5	96,781,584 (GRCm38)	missense	probably damaging	0.96
R8000:Fras1	UTSW	5	96,762,677 (GRCm38)	missense	probably damaging	0.96
R8056:Fras1	UTSW	5	96,744,774 (GRCm38)	missense	probably damaging	1.00
R8058:Fras1	UTSW	5	96,694,919 (GRCm38)	missense	probably benign
R8117:Fras1	UTSW	5	96,707,386 (GRCm38)	missense	probably damaging	1.00
R8157:Fras1	UTSW	5	96,554,855 (GRCm38)	missense	probably benign	0.00
R8312:Fras1	UTSW	5	96,588,191 (GRCm38)	missense	probably benign
R8315:Fras1	UTSW	5	96,743,182 (GRCm38)	missense	probably damaging	0.97
R8412:Fras1	UTSW	5	96,596,852 (GRCm38)	missense	probably benign	0.00
R8546:Fras1	UTSW	5	96,709,966 (GRCm38)	missense	probably benign	0.00
R8705:Fras1	UTSW	5	96,691,401 (GRCm38)	missense	probably benign	0.01
R8790:Fras1	UTSW	5	96,755,377 (GRCm38)	missense	probably damaging	1.00
R8848:Fras1	UTSW	5	96,781,348 (GRCm38)	missense	probably damaging	1.00
R8855:Fras1	UTSW	5	96,769,606 (GRCm38)	missense
R8871:Fras1	UTSW	5	96,707,398 (GRCm38)	missense	probably benign	0.34
R8894:Fras1	UTSW	5	96,759,543 (GRCm38)	missense	probably damaging	1.00
R8904:Fras1	UTSW	5	96,781,279 (GRCm38)	missense	probably benign	0.16
R8910:Fras1	UTSW	5	96,567,996 (GRCm38)	missense	probably benign	0.01
R8916:Fras1	UTSW	5	96,752,915 (GRCm38)	missense	probably damaging	1.00
R8929:Fras1	UTSW	5	96,769,507 (GRCm38)	missense	probably damaging	0.98
R8995:Fras1	UTSW	5	96,712,556 (GRCm38)	missense	possibly damaging	0.83
R9016:Fras1	UTSW	5	96,636,064 (GRCm38)	missense	probably damaging	0.96
R9021:Fras1	UTSW	5	96,740,750 (GRCm38)	missense	probably damaging	1.00
R9038:Fras1	UTSW	5	96,726,883 (GRCm38)	missense	probably benign	0.25
R9204:Fras1	UTSW	5	96,735,163 (GRCm38)	missense	probably damaging	1.00
R9222:Fras1	UTSW	5	96,657,228 (GRCm38)	missense	probably benign	0.15
R9231:Fras1	UTSW	5	96,545,045 (GRCm38)	missense	probably damaging	1.00
R9238:Fras1	UTSW	5	96,684,361 (GRCm38)	missense	possibly damaging	0.90
R9257:Fras1	UTSW	5	96,762,500 (GRCm38)	missense	probably damaging	1.00
R9302:Fras1	UTSW	5	96,534,892 (GRCm38)	missense	probably damaging	1.00
R9329:Fras1	UTSW	5	96,736,954 (GRCm38)	missense	probably damaging	1.00
R9361:Fras1	UTSW	5	96,776,698 (GRCm38)	missense	probably damaging	1.00
R9430:Fras1	UTSW	5	96,781,392 (GRCm38)	missense	probably benign	0.00
R9431:Fras1	UTSW	5	96,753,014 (GRCm38)	missense	possibly damaging	0.71
R9474:Fras1	UTSW	5	96,739,265 (GRCm38)	missense	probably benign	0.00
R9475:Fras1	UTSW	5	96,780,070 (GRCm38)	missense	probably damaging	0.96
R9497:Fras1	UTSW	5	96,737,036 (GRCm38)	missense	probably damaging	1.00
R9597:Fras1	UTSW	5	96,740,692 (GRCm38)	missense	probably damaging	1.00
R9650:Fras1	UTSW	5	96,762,528 (GRCm38)	missense	probably damaging	1.00
R9694:Fras1	UTSW	5	96,781,686 (GRCm38)	missense	probably benign	0.03
R9779:Fras1	UTSW	5	96,569,494 (GRCm38)	missense	probably damaging	0.99
Z1088:Fras1	UTSW	5	96,743,211 (GRCm38)	missense	probably damaging	0.97
Z1088:Fras1	UTSW	5	96,758,142 (GRCm38)	missense	probably benign
Z1176:Fras1	UTSW	5	96,758,142 (GRCm38)	missense	probably benign
Z1177:Fras1	UTSW	5	96,700,251 (GRCm38)	missense	possibly damaging	0.71
Z1177:Fras1	UTSW	5	96,691,457 (GRCm38)	missense	probably damaging	1.00
Z1177:Fras1	UTSW	5	96,758,142 (GRCm38)	missense	probably benign
Z1177:Fras1	UTSW	5	96,740,983 (GRCm38)	missense	possibly damaging	0.95
Z1177:Fras1	UTSW	5	96,740,811 (GRCm38)	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- TCAGACCAAGTCACTGCTTC -3'
(R):5'- CAGTACAGCCAAGACCCTTG -3'

Sequencing Primer
(F):5'- AGACCAAGTCACTGCTTCTTGTG -3'
(R):5'- GACCAAGGCACTTCTAGA -3'

Posted On

2019-10-17