Mutagenetix > Incidental Mutation

Incidental Mutation 'R7566:Zfp945'

585469

Institutional Source

Beutler Lab

Gene Symbol

Zfp945

Ensembl Gene

ENSMUSG00000059142

Gene Name

zinc finger protein 945

Synonyms

C730040L01Rik, A630033E08Rik

MMRRC Submission

045628-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

R7566 (G1)

Quality Score

170.009

Status

Validated

Chromosome

Chromosomal Location

23065671-23086108 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 23070727 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 412 (C412S)

Ref Sequence

ENSEMBL: ENSMUSP00000124123 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088696] [ENSMUST00000150092] [ENSMUST00000160457]

AlphaFold

F6WAU7

Predicted Effect

probably benign
Transcript: ENSMUST00000088696
AA Change: C391S

PolyPhen 2 Score 0.109 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000086074
Gene: ENSMUSG00000059142
AA Change: C391S

Domain	Start	End	E-Value	Type
KRAB	13	68	9.12e-18	SMART
ZnF_C2H2	210	232	1.45e-2	SMART
ZnF_C2H2	238	260	1.03e-2	SMART
ZnF_C2H2	266	288	1.76e-1	SMART
ZnF_C2H2	322	344	7.37e-4	SMART
ZnF_C2H2	350	372	1.5e-4	SMART
ZnF_C2H2	378	400	5.14e-3	SMART
ZnF_C2H2	406	428	9.73e-4	SMART
ZnF_C2H2	434	456	2.99e-4	SMART
ZnF_C2H2	462	484	4.54e-4	SMART
ZnF_C2H2	490	512	2.4e-3	SMART
ZnF_C2H2	518	540	1.38e-3	SMART
ZnF_C2H2	546	568	1.13e-4	SMART
ZnF_C2H2	574	596	5.59e-4	SMART
ZnF_C2H2	602	624	9.31e1	SMART
ZnF_C2H2	630	652	7.9e-4	SMART
ZnF_C2H2	658	680	3.69e-4	SMART
ZnF_C2H2	686	708	3.16e-3	SMART
ZnF_C2H2	714	736	2.95e-3	SMART
ZnF_C2H2	742	764	1.98e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000150092

SMART Domains

Protein: ENSMUSP00000117978
Gene: ENSMUSG00000059142

Domain	Start	End	E-Value	Type
KRAB	34	89	9.12e-18	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000160457
AA Change: C412S

PolyPhen 2 Score 0.711 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000124123
Gene: ENSMUSG00000059142
AA Change: C412S

Domain	Start	End	E-Value	Type
KRAB	34	89	9.12e-18	SMART
ZnF_C2H2	231	253	1.45e-2	SMART
ZnF_C2H2	259	281	1.03e-2	SMART
ZnF_C2H2	287	309	1.76e-1	SMART
ZnF_C2H2	343	365	7.37e-4	SMART
ZnF_C2H2	371	393	1.5e-4	SMART
ZnF_C2H2	399	421	5.14e-3	SMART
ZnF_C2H2	427	449	9.73e-4	SMART
ZnF_C2H2	455	477	2.99e-4	SMART
ZnF_C2H2	483	505	4.54e-4	SMART
ZnF_C2H2	511	533	2.4e-3	SMART
ZnF_C2H2	539	561	1.38e-3	SMART
ZnF_C2H2	567	589	1.13e-4	SMART
ZnF_C2H2	595	617	5.59e-4	SMART
ZnF_C2H2	623	645	9.31e1	SMART
ZnF_C2H2	651	673	7.9e-4	SMART
ZnF_C2H2	679	701	3.69e-4	SMART
ZnF_C2H2	707	729	3.16e-3	SMART
ZnF_C2H2	735	757	2.95e-3	SMART
ZnF_C2H2	763	785	1.98e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

100% (57/57)

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A830018L16Rik	T	G	1: 12,021,252 (GRCm39)	S420R	probably damaging	Het
Acat2	C	T	17: 13,166,346 (GRCm39)	D219N	probably damaging	Het
Anapc10	G	A	8: 80,446,308 (GRCm39)	R46Q	possibly damaging	Het
Ankub1	G	A	3: 57,573,039 (GRCm39)	R228*	probably null	Het
Arhgap21	T	C	2: 20,917,102 (GRCm39)	N91S	probably benign	Het
Arhgap32	T	A	9: 32,162,018 (GRCm39)	V323D	probably benign	Het
Bcas1	C	T	2: 170,212,369 (GRCm39)		probably null	Het
Cd109	C	T	9: 78,588,119 (GRCm39)	P716S	probably damaging	Het
Chd4	C	T	6: 125,078,866 (GRCm39)	P267L	possibly damaging	Het
Cpne7	G	T	8: 123,860,552 (GRCm39)	V502F	probably damaging	Het
Cracd	G	A	5: 77,014,122 (GRCm39)		probably null	Het
Dlgap4	C	T	2: 156,604,657 (GRCm39)	A921V	probably benign	Het
Faap24	G	T	7: 35,092,465 (GRCm39)	R151S	probably benign	Het
Fam98a	A	G	17: 75,854,657 (GRCm39)	S51P	probably damaging	Het
Fbxl8	A	G	8: 105,994,938 (GRCm39)	D150G	possibly damaging	Het
Fut10	A	G	8: 31,749,950 (GRCm39)	K412R	probably benign	Het
G0s2	T	C	1: 192,955,076 (GRCm39)	S3G	probably benign	Het
Gm1527	T	C	3: 28,974,767 (GRCm39)	Y527H	probably benign	Het
Gsdmc3	T	C	15: 63,733,510 (GRCm39)	K227R	possibly damaging	Het
Gtf2ird2	A	G	5: 134,242,848 (GRCm39)	Y354C	probably damaging	Het
Hmcn2	T	A	2: 31,344,869 (GRCm39)	N4685K	probably damaging	Het
Itgad	A	T	7: 127,791,279 (GRCm39)	Y717F	probably benign	Het
Kcnh5	C	T	12: 75,161,166 (GRCm39)	W247*	probably null	Het
Kcnt1	C	A	2: 25,806,048 (GRCm39)	S1212R	probably benign	Het
Kirrel1	A	G	3: 86,995,791 (GRCm39)	V381A	probably damaging	Het
Loxl1	T	A	9: 58,219,481 (GRCm39)	Q230L	probably damaging	Het
Magi1	T	C	6: 93,655,308 (GRCm39)	E1446G	probably benign	Het
Man1a	A	G	10: 53,795,330 (GRCm39)	V550A	possibly damaging	Het
Mgat4d	T	C	8: 84,084,652 (GRCm39)	S132P	probably damaging	Het
Muc16	T	A	9: 18,549,925 (GRCm39)	H5456L	probably benign	Het
Nhsl1	A	G	10: 18,391,867 (GRCm39)	K207R	probably damaging	Het
Nlrp4a	A	C	7: 26,148,670 (GRCm39)		probably null	Het
Nr6a1	T	C	2: 38,621,085 (GRCm39)	M407V	possibly damaging	Het
Or13c7b	A	G	4: 43,820,711 (GRCm39)	S217P	probably damaging	Het
Or2b2b	T	G	13: 21,858,737 (GRCm39)	I126L	possibly damaging	Het
Or2z2	G	T	11: 58,346,489 (GRCm39)	F95L	probably benign	Het
Or6c206	T	C	10: 129,097,469 (GRCm39)	I213T	probably damaging	Het
Ovgp1	A	G	3: 105,881,626 (GRCm39)		probably benign	Het
Ovol3	G	T	7: 29,933,791 (GRCm39)	F110L	probably damaging	Het
Pcsk5	T	C	19: 17,549,821 (GRCm39)	T724A	probably benign	Het
Phtf2	G	T	5: 20,970,799 (GRCm39)	T653N	probably damaging	Het
Plch1	A	T	3: 63,688,663 (GRCm39)		probably null	Het
Prkce	A	T	17: 86,800,757 (GRCm39)	E391V	probably benign	Het
Prob1	A	G	18: 35,788,038 (GRCm39)	V72A	probably benign	Het
Prrc2b	C	A	2: 32,084,402 (GRCm39)	P289Q	probably benign	Het
Ptbp3	A	T	4: 59,514,280 (GRCm39)	N114K	probably benign	Het
Rho	T	A	6: 115,909,135 (GRCm39)	L57H	probably damaging	Het
Slc44a5	A	C	3: 153,975,626 (GRCm39)	D679A	probably damaging	Het
Slit2	A	T	5: 48,407,239 (GRCm39)	D898V	probably damaging	Het
Spag16	C	T	1: 69,909,487 (GRCm39)	R195C	probably damaging	Het
Tmprss11a	T	G	5: 86,591,993 (GRCm39)	D60A	possibly damaging	Het
Ttn	T	A	2: 76,565,293 (GRCm39)	R28311S	probably damaging	Het
Wfikkn1	T	C	17: 26,097,352 (GRCm39)	D324G	probably damaging	Het
Zan	A	C	5: 137,410,845 (GRCm39)		probably null	Het

Other mutations in Zfp945

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00754:Zfp945	APN	17	23,070,931 (GRCm39)	unclassified	probably benign
IGL01077:Zfp945	APN	17	23,071,359 (GRCm39)	missense	probably damaging	0.99
IGL02706:Zfp945	APN	17	23,076,256 (GRCm39)	missense	probably damaging	1.00
IGL03408:Zfp945	APN	17	23,071,511 (GRCm39)	nonsense	probably null
Small-scale	UTSW	17	23,069,955 (GRCm39)	missense	probably damaging	1.00
R0427:Zfp945	UTSW	17	23,084,226 (GRCm39)	missense	probably benign	0.10
R0718:Zfp945	UTSW	17	23,070,004 (GRCm39)	missense	probably damaging	1.00
R1305:Zfp945	UTSW	17	23,071,360 (GRCm39)	missense	probably damaging	0.97
R1801:Zfp945	UTSW	17	23,070,736 (GRCm39)	missense	probably damaging	0.97
R1837:Zfp945	UTSW	17	23,070,247 (GRCm39)	missense	probably damaging	1.00
R2001:Zfp945	UTSW	17	23,076,223 (GRCm39)	critical splice donor site	probably null
R4193:Zfp945	UTSW	17	23,070,144 (GRCm39)	unclassified	probably benign
R4247:Zfp945	UTSW	17	23,069,583 (GRCm39)	missense	probably damaging	0.98
R5026:Zfp945	UTSW	17	23,069,859 (GRCm39)	missense	probably damaging	1.00
R5918:Zfp945	UTSW	17	23,069,955 (GRCm39)	missense	probably damaging	1.00
R6076:Zfp945	UTSW	17	23,070,432 (GRCm39)	missense	probably damaging	1.00
R6664:Zfp945	UTSW	17	23,071,339 (GRCm39)	missense	probably damaging	0.99
R6997:Zfp945	UTSW	17	23,071,543 (GRCm39)	nonsense	probably null
R7040:Zfp945	UTSW	17	23,071,264 (GRCm39)	missense	probably damaging	0.98
R7479:Zfp945	UTSW	17	23,070,340 (GRCm39)	missense	possibly damaging	0.93
R7948:Zfp945	UTSW	17	23,071,096 (GRCm39)	missense	unknown
R8383:Zfp945	UTSW	17	23,070,798 (GRCm39)	missense	probably benign	0.05
R9776:Zfp945	UTSW	17	23,070,582 (GRCm39)	missense	possibly damaging	0.93
R9790:Zfp945	UTSW	17	23,071,228 (GRCm39)	missense	probably benign	0.33
R9791:Zfp945	UTSW	17	23,071,228 (GRCm39)	missense	probably benign	0.33
X0063:Zfp945	UTSW	17	23,071,202 (GRCm39)	unclassified	probably benign

Predicted Primers

PCR Primer
(F):5'- GATGAATTCTAAGGTGGTCTTTCC -3'
(R):5'- GTGAATGCCATTACCGTGCTG -3'

Sequencing Primer
(F):5'- CCGGTGGAAGGATTTGTCACAG -3'
(R):5'- GCCATTACCGTGCTGATATAAG -3'

Posted On

2019-10-17