Mutagenetix > Incidental Mutation

Incidental Mutation 'R7571:Vmn2r5'

585792

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r5

Ensembl Gene

ENSMUSG00000068999

Gene Name

vomeronasal 2, receptor 5

Synonyms

EG667060

MMRRC Submission

045711-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.122) question?

Stock #

R7571 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

64490821-64509735 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 64504404 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 248 (C248S)

Ref Sequence

ENSEMBL: ENSMUSP00000135592 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170270] [ENSMUST00000177184]

AlphaFold

K7N788

Predicted Effect

probably damaging
Transcript: ENSMUST00000170270
AA Change: C161S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000131925
Gene: ENSMUSG00000068999
AA Change: C161S

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	9.9e-78	PFAM
Pfam:NCD3G	458	511	8.5e-18	PFAM
Pfam:7tm_3	542	779	2.3e-74	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000177184
AA Change: C248S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000135592
Gene: ENSMUSG00000068999
AA Change: C248S

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	86	503	1.8e-80	PFAM
Pfam:NCD3G	545	598	1e-14	PFAM
Pfam:7tm_3	631	865	1.4e-45	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.8%
20x: 99.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 112 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700001C02Rik	A	T	5: 30,482,158 (GRCm38)	E143V	possibly damaging	Het
Abca16	A	T	7: 120,519,988 (GRCm38)	N985I	probably benign	Het
Abi3bp	T	A	16: 56,630,982 (GRCm38)		probably null	Het
Acad12	A	T	5: 121,607,194 (GRCm38)	Y309*	probably null	Het
Acadsb	G	T	7: 131,443,554 (GRCm38)	R405L	probably damaging	Het
Acan	G	A	7: 79,086,267 (GRCm38)	V154M	probably damaging	Het
Adam22	G	A	5: 8,082,160 (GRCm38)	R894*	probably null	Het
Ahnak	A	T	19: 9,000,786 (GRCm38)	K90*	probably null	Het
Ak4	T	C	4: 101,460,542 (GRCm38)	I103T	probably benign	Het
Ap4e1	C	T	2: 127,019,336 (GRCm38)	L132F	probably damaging	Het
Apol7b	C	T	15: 77,423,477 (GRCm38)	V273I	probably benign	Het
Arhgef37	T	A	18: 61,504,332 (GRCm38)	I420F	probably damaging	Het
Atg13	C	T	2: 91,680,342 (GRCm38)		probably null	Het
Bach1	G	A	16: 87,719,291 (GRCm38)	R240Q	probably benign	Het
Cabin1	A	T	10: 75,646,666 (GRCm38)	S2148T	probably damaging	Het
Cacna1i	T	C	15: 80,375,336 (GRCm38)	V1269A	probably damaging	Het
Cdipt	T	C	7: 126,979,622 (GRCm38)	I195T	probably benign	Het
Ces2b	A	T	8: 104,835,009 (GRCm38)	H245L	probably damaging	Het
Clstn1	T	G	4: 149,646,287 (GRCm38)	M825R	probably benign	Het
Cntn3	G	A	6: 102,278,403 (GRCm38)	T178I	probably damaging	Het
Col7a1	A	G	9: 108,982,707 (GRCm38)	M2789V	probably null	Het
Col9a2	A	T	4: 121,039,784 (GRCm38)	I24F	unknown	Het
Cpne2	T	C	8: 94,551,780 (GRCm38)	M134T	probably benign	Het
Cpt1b	A	G	15: 89,421,343 (GRCm38)		probably null	Het
Crocc	T	C	4: 141,046,049 (GRCm38)		probably null	Het
Cts8	T	C	13: 61,248,167 (GRCm38)	Y330C	probably damaging	Het
Cwc22	T	C	2: 77,917,067 (GRCm38)	D434G	probably benign	Het
Cyp3a13	T	C	5: 137,898,863 (GRCm38)	I396M	possibly damaging	Het
D430042O09Rik	C	T	7: 125,708,021 (GRCm38)		probably benign	Het
D5Ertd577e	A	T	5: 95,482,960 (GRCm38)	N232I	probably damaging	Het
Ddr2	A	T	1: 170,001,851 (GRCm38)	I278N	probably benign	Het
Dnaaf5	A	G	5: 139,170,208 (GRCm38)	E548G	possibly damaging	Het
Dync2h1	A	G	9: 7,002,623 (GRCm38)	L3808P	probably damaging	Het
Faf2	T	A	13: 54,650,214 (GRCm38)	W209R	probably damaging	Het
Fam129a	T	C	1: 151,718,297 (GRCm38)	V911A	probably benign	Het
Fam208b	T	C	13: 3,575,292 (GRCm38)	T1553A	probably benign	Het
Fbln2	A	T	6: 91,268,575 (GRCm38)	E992D	probably damaging	Het
Fdxacb1	T	A	9: 50,771,793 (GRCm38)	V352D	probably damaging	Het
Flg2	G	T	3: 93,219,996 (GRCm38)	G2072*	probably null	Het
Flii	A	T	11: 60,721,136 (GRCm38)	L347Q	probably damaging	Het
Fndc3a	A	T	14: 72,589,896 (GRCm38)	H116Q	probably damaging	Het
Fnta	A	T	8: 26,015,465 (GRCm38)	M39K	probably benign	Het
Gabra4	A	G	5: 71,571,992 (GRCm38)	S482P	probably benign	Het
Gapdhs	G	A	7: 30,737,958 (GRCm38)	P61S	unknown	Het
Gm10153	A	T	7: 142,189,664 (GRCm38)	C242*	probably null	Het
Gp1ba	A	G	11: 70,640,094 (GRCm38)	I229V	unknown	Het
Grap2	T	A	15: 80,643,704 (GRCm38)	L117Q	probably damaging	Het
Grik5	A	G	7: 25,013,885 (GRCm38)	I766T	possibly damaging	Het
Hrh3	A	T	2: 180,101,286 (GRCm38)	I183N	probably damaging	Het
Htr5a	C	A	5: 27,842,895 (GRCm38)	Y149*	probably null	Het
Ibtk	T	C	9: 85,722,300 (GRCm38)	I527V	probably benign	Het
Il33	T	A	19: 29,956,941 (GRCm38)	S184R	probably damaging	Het
Kansl3	T	A	1: 36,365,587 (GRCm38)	Q94L	possibly damaging	Het
Kcnh6	A	G	11: 106,017,416 (GRCm38)	D286G	probably benign	Het
Kmt2e	T	A	5: 23,478,587 (GRCm38)	M281K	probably damaging	Het
Lrp2	T	A	2: 69,516,403 (GRCm38)	H833L	probably damaging	Het
Meis1	A	T	11: 18,941,702 (GRCm38)	V282E	probably damaging	Het
Mfsd8	T	G	3: 40,830,662 (GRCm38)	D240A	probably damaging	Het
Muc5b	A	G	7: 141,847,249 (GRCm38)	T534A	unknown	Het
Myo18b	G	A	5: 112,830,328 (GRCm38)	L1243F	probably damaging	Het
Nefl	A	G	14: 68,084,674 (GRCm38)	I238V	probably benign	Het
Nexn	T	A	3: 152,253,647 (GRCm38)	I62F	possibly damaging	Het
Notch4	T	C	17: 34,583,574 (GRCm38)	L1323P	probably damaging	Het
Olfr493	A	T	7: 108,346,482 (GRCm38)	F166L	probably benign	Het
Olfr555	T	C	7: 102,659,051 (GRCm38)	S77P	probably damaging	Het
Olfr906	T	C	9: 38,488,656 (GRCm38)	V209A	probably benign	Het
Patj	C	A	4: 98,568,980 (GRCm38)	H1244N	probably damaging	Het
Peg10	T	A	6: 4,756,082 (GRCm38)	D219E	unknown	Het
Pi4kb	G	T	3: 94,999,114 (GRCm38)		probably null	Het
Piezo1	A	G	8: 122,498,418 (GRCm38)	F614S		Het
Pisd	T	A	5: 32,737,337 (GRCm38)	N372Y	probably damaging	Het
Piwil4	T	C	9: 14,734,597 (GRCm38)	D178G	probably benign	Het
Plekho1	G	T	3: 95,989,254 (GRCm38)	P301Q	probably damaging	Het
Pop1	T	A	15: 34,528,947 (GRCm38)	W738R	probably null	Het
Ppp4r1	C	T	17: 65,810,616 (GRCm38)	P125L	possibly damaging	Het
Prdm9	T	A	17: 15,563,264 (GRCm38)	N13I	probably damaging	Het
Psd4	T	G	2: 24,407,011 (GRCm38)	V932G	probably damaging	Het
Ptger3	T	C	3: 157,641,775 (GRCm38)	S355P	probably benign	Het
Ptprb	T	C	10: 116,339,430 (GRCm38)	V823A	probably damaging	Het
Ptprj	C	T	2: 90,455,186 (GRCm38)	V841I	probably benign	Het
Ranbp3	T	C	17: 56,707,923 (GRCm38)	S281P	probably benign	Het
Rasal3	T	A	17: 32,395,861 (GRCm38)	M541L	possibly damaging	Het
Rassf1	A	G	9: 107,551,783 (GRCm38)	T63A	possibly damaging	Het
Rcor3	C	A	1: 192,137,876 (GRCm38)	G8V	probably damaging	Het
Rgs4	G	A	1: 169,744,358 (GRCm38)	T124I	probably damaging	Het
Rspo2	T	A	15: 43,169,976 (GRCm38)		probably benign	Het
Scgn	T	C	13: 23,953,914 (GRCm38)	D258G	probably damaging	Het
Shkbp1	A	G	7: 27,347,131 (GRCm38)	S403P	possibly damaging	Het
Slc25a13	A	G	6: 6,052,785 (GRCm38)	L525P	probably damaging	Het
Snd1	G	T	6: 28,526,203 (GRCm38)	K193N	possibly damaging	Het
Sntg2	C	T	12: 30,175,202 (GRCm38)	A516T	probably damaging	Het
Stard13	A	G	5: 151,059,502 (GRCm38)	I699T	probably damaging	Het
Svil	T	A	18: 5,114,636 (GRCm38)	V1984D	probably damaging	Het
Swt1	A	G	1: 151,394,719 (GRCm38)	S582P	probably benign	Het
Sypl2	A	G	3: 108,214,538 (GRCm38)	*265Q	probably null	Het
Taar8a	T	A	10: 24,077,408 (GRCm38)	Y303*	probably null	Het
Tlr2	T	A	3: 83,836,542 (GRCm38)	I745F	probably damaging	Het
Tnrc6b	A	G	15: 80,929,393 (GRCm38)	T1784A	probably benign	Het
Tpo	C	A	12: 30,119,432 (GRCm38)	M101I	probably benign	Het
Trpm2	G	T	10: 77,937,950 (GRCm38)	R544S	probably benign	Het
Ttc14	A	G	3: 33,809,251 (GRCm38)	R603G	unknown	Het
Tubgcp6	A	C	15: 89,100,722 (GRCm38)	V1694G	probably damaging	Het
Uba6	C	A	5: 86,147,111 (GRCm38)	K356N	probably benign	Het
Usp16	T	C	16: 87,464,835 (GRCm38)	V113A	possibly damaging	Het
Vcam1	G	A	3: 116,114,383 (GRCm38)	Q677*	probably null	Het
Vmn1r238	T	A	18: 3,122,721 (GRCm38)	E231V	probably damaging	Het
Vmn1r70	T	C	7: 10,633,944 (GRCm38)	F120L	probably benign	Het
Vmn2r116	T	A	17: 23,384,856 (GRCm38)		probably null	Het
Vmn2r87	A	T	10: 130,479,071 (GRCm38)	D215E	probably damaging	Het
Xkr4	T	G	1: 3,670,688 (GRCm38)	I221L	probably benign	Het
Zfp366	T	C	13: 99,246,387 (GRCm38)	I686T	probably benign	Het
Zfp398	T	A	6: 47,866,732 (GRCm38)	C573S	probably damaging	Het

Other mutations in Vmn2r5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00742:Vmn2r5	APN	3	64,491,413 (GRCm38)	missense	possibly damaging	0.67
IGL01139:Vmn2r5	APN	3	64,491,405 (GRCm38)	missense	probably benign	0.28
IGL03293:Vmn2r5	APN	3	64,491,326 (GRCm38)	missense	probably benign	0.01
PIT4581001:Vmn2r5	UTSW	3	64,503,927 (GRCm38)	nonsense	probably null
R0063:Vmn2r5	UTSW	3	64,503,800 (GRCm38)	missense	probably benign	0.01
R0142:Vmn2r5	UTSW	3	64,492,588 (GRCm38)	missense	probably damaging	1.00
R0193:Vmn2r5	UTSW	3	64,491,530 (GRCm38)	missense	possibly damaging	0.91
R0219:Vmn2r5	UTSW	3	64,504,313 (GRCm38)	missense	probably damaging	0.99
R0313:Vmn2r5	UTSW	3	64,503,827 (GRCm38)	missense	probably benign
R0620:Vmn2r5	UTSW	3	64,503,814 (GRCm38)	nonsense	probably null
R0726:Vmn2r5	UTSW	3	64,503,765 (GRCm38)	missense	probably benign	0.01
R1073:Vmn2r5	UTSW	3	64,491,305 (GRCm38)	nonsense	probably null
R1549:Vmn2r5	UTSW	3	64,504,000 (GRCm38)	missense	probably damaging	1.00
R1581:Vmn2r5	UTSW	3	64,491,219 (GRCm38)	missense	probably damaging	1.00
R1624:Vmn2r5	UTSW	3	64,509,695 (GRCm38)	missense	probably benign	0.19
R1973:Vmn2r5	UTSW	3	64,504,221 (GRCm38)	missense	probably damaging	1.00
R1974:Vmn2r5	UTSW	3	64,504,221 (GRCm38)	missense	probably damaging	1.00
R1975:Vmn2r5	UTSW	3	64,504,221 (GRCm38)	missense	probably damaging	1.00
R1977:Vmn2r5	UTSW	3	64,504,221 (GRCm38)	missense	probably damaging	1.00
R2189:Vmn2r5	UTSW	3	64,509,593 (GRCm38)	missense	probably benign	0.27
R2484:Vmn2r5	UTSW	3	64,503,971 (GRCm38)	missense	possibly damaging	0.94
R4573:Vmn2r5	UTSW	3	64,503,918 (GRCm38)	missense	probably damaging	0.98
R5243:Vmn2r5	UTSW	3	64,504,076 (GRCm38)	missense	probably damaging	1.00
R5384:Vmn2r5	UTSW	3	64,509,510 (GRCm38)	missense	probably benign	0.02
R5385:Vmn2r5	UTSW	3	64,509,510 (GRCm38)	missense	probably benign	0.02
R5587:Vmn2r5	UTSW	3	64,504,076 (GRCm38)	missense	probably damaging	1.00
R5588:Vmn2r5	UTSW	3	64,504,076 (GRCm38)	missense	probably damaging	1.00
R5589:Vmn2r5	UTSW	3	64,504,076 (GRCm38)	missense	probably damaging	1.00
R5590:Vmn2r5	UTSW	3	64,504,076 (GRCm38)	missense	probably damaging	1.00
R5739:Vmn2r5	UTSW	3	64,504,076 (GRCm38)	missense	probably damaging	1.00
R6113:Vmn2r5	UTSW	3	64,491,399 (GRCm38)	missense	probably benign	0.03
R6739:Vmn2r5	UTSW	3	64,491,216 (GRCm38)	missense	probably damaging	0.97
R7106:Vmn2r5	UTSW	3	64,491,683 (GRCm38)	missense	probably benign	0.05
R7304:Vmn2r5	UTSW	3	64,504,250 (GRCm38)	missense	probably damaging	0.97
R7402:Vmn2r5	UTSW	3	64,495,755 (GRCm38)	missense	probably benign	0.05
R7477:Vmn2r5	UTSW	3	64,491,639 (GRCm38)	missense	probably damaging	1.00
R7678:Vmn2r5	UTSW	3	64,509,522 (GRCm38)	missense	probably benign
R7874:Vmn2r5	UTSW	3	64,491,032 (GRCm38)	missense	probably damaging	1.00
R8110:Vmn2r5	UTSW	3	64,491,288 (GRCm38)	missense	probably benign	0.00
R8223:Vmn2r5	UTSW	3	64,491,305 (GRCm38)	nonsense	probably null
R8767:Vmn2r5	UTSW	3	64,507,682 (GRCm38)	missense	possibly damaging	0.79
R8896:Vmn2r5	UTSW	3	64,503,782 (GRCm38)	missense	probably benign	0.00
R8948:Vmn2r5	UTSW	3	64,491,101 (GRCm38)	missense	probably damaging	1.00
R8950:Vmn2r5	UTSW	3	64,491,101 (GRCm38)	missense	probably damaging	1.00
R8962:Vmn2r5	UTSW	3	64,491,143 (GRCm38)	missense	probably damaging	1.00
R9012:Vmn2r5	UTSW	3	64,504,494 (GRCm38)	missense	probably damaging	1.00
R9109:Vmn2r5	UTSW	3	64,503,990 (GRCm38)	missense	possibly damaging	0.58
R9126:Vmn2r5	UTSW	3	64,491,738 (GRCm38)	missense	probably benign	0.13
R9192:Vmn2r5	UTSW	3	64,491,517 (GRCm38)	nonsense	probably null
R9221:Vmn2r5	UTSW	3	64,504,300 (GRCm38)	nonsense	probably null
R9375:Vmn2r5	UTSW	3	64,503,895 (GRCm38)	missense	probably damaging	1.00
R9529:Vmn2r5	UTSW	3	64,492,597 (GRCm38)	missense	probably benign	0.06
Z1088:Vmn2r5	UTSW	3	64,509,542 (GRCm38)	missense	probably benign	0.02
Z1177:Vmn2r5	UTSW	3	64,491,051 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCTGAGGTAATCCAGGCTTC -3'
(R):5'- CCAGCGCAATTCTTAGTGAC -3'

Sequencing Primer
(F):5'- AGGTAATCCAGGCTTCGCTGG -3'
(R):5'- CCAGCGCAATTCTTAGTGACAAATTC -3'

Posted On

2019-10-17