Mutagenetix > Incidental Mutations

Incidental Mutation 'R0620:Zfp341'

58580

Institutional Source

Beutler Lab

Gene Symbol

Zfp341

Ensembl Gene

ENSMUSG00000059842

Gene Name

zinc finger protein 341

Synonyms

MMRRC Submission

038809-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0620 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

154613297-154646821 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 154634273 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 460 (E460G)

Ref Sequence

ENSEMBL: ENSMUSP00000105324 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081926] [ENSMUST00000109702] [ENSMUST00000126421]

AlphaFold

Q6PGC9

Predicted Effect

possibly damaging
Transcript: ENSMUST00000081926
AA Change: E467G

PolyPhen 2 Score 0.903 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000080596
Gene: ENSMUSG00000059842
AA Change: E467G

Domain	Start	End	E-Value	Type
ZnF_C2H2	53	73	4.16e1	SMART
low complexity region	96	110	N/A	INTRINSIC
low complexity region	154	161	N/A	INTRINSIC
low complexity region	177	200	N/A	INTRINSIC
ZnF_C2H2	320	342	3.69e-4	SMART
ZnF_C2H2	348	370	1.04e-3	SMART
ZnF_C2H2	442	464	1.45e-2	SMART
ZnF_C2H2	470	494	2.17e-1	SMART
ZnF_C2H2	500	522	2.91e-2	SMART
ZnF_C2H2	537	561	1.23e0	SMART
ZnF_C2H2	563	585	3.58e-2	SMART
ZnF_C2H2	591	613	1.62e0	SMART
ZnF_C2H2	619	641	2.27e-4	SMART
ZnF_C2H2	647	674	7.29e0	SMART
ZnF_C2H2	680	702	5.14e-3	SMART
low complexity region	740	759	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000109702
AA Change: E460G

PolyPhen 2 Score 0.944 (Sensitivity: 0.80; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000105324
Gene: ENSMUSG00000059842
AA Change: E460G

Domain	Start	End	E-Value	Type
ZnF_C2H2	53	73	4.16e1	SMART
low complexity region	96	110	N/A	INTRINSIC
low complexity region	154	161	N/A	INTRINSIC
low complexity region	177	200	N/A	INTRINSIC
ZnF_C2H2	313	335	3.69e-4	SMART
ZnF_C2H2	341	363	1.04e-3	SMART
ZnF_C2H2	435	457	1.45e-2	SMART
ZnF_C2H2	463	487	2.17e-1	SMART
ZnF_C2H2	493	515	2.91e-2	SMART
ZnF_C2H2	530	554	1.23e0	SMART
ZnF_C2H2	556	578	3.58e-2	SMART
ZnF_C2H2	584	606	1.62e0	SMART
ZnF_C2H2	612	634	2.27e-4	SMART
ZnF_C2H2	640	667	7.29e0	SMART
ZnF_C2H2	673	695	5.14e-3	SMART
low complexity region	733	752	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000126421

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153208

Meta Mutation Damage Score

0.4553

Coding Region Coverage

1x: 99.4%
3x: 99.0%
10x: 97.8%
20x: 96.1%

Validation Efficiency

100% (74/74)

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2810474O19Rik	A	T	6: 149,328,375	Q973L	probably damaging	Het
Adamts9	T	C	6: 92,858,113	T679A	possibly damaging	Het
Ahr	T	C	12: 35,508,194	T276A	probably benign	Het
Akap9	A	T	5: 4,064,136	Q3138H	probably damaging	Het
Armt1	T	A	10: 4,432,689	F7I	probably benign	Het
B3galt2	A	G	1: 143,646,140	R5G	probably damaging	Het
Bod1l	T	C	5: 41,801,233	N2750S	probably benign	Het
Cadps2	T	A	6: 23,583,396	E365V	probably damaging	Het
Cd200r3	T	A	16: 44,957,717		probably null	Het
Cst7	T	A	2: 150,575,886		probably benign	Het
Defb30	A	T	14: 63,049,763		probably benign	Het
Dido1	C	T	2: 180,659,851	G2087S	probably benign	Het
Dio2	A	G	12: 90,738,071	Y72H	probably benign	Het
Dnah11	C	T	12: 117,987,469	E3035K	probably damaging	Het
Dnajb13	T	C	7: 100,503,249	K287E	possibly damaging	Het
Dnajc11	G	A	4: 151,973,628	V244I	possibly damaging	Het
Ect2	C	T	3: 27,139,652	A226T	probably damaging	Het
Ercc8	G	A	13: 108,174,061		probably null	Het
Fam120b	T	A	17: 15,402,927	M389K	probably benign	Het
Fam151a	A	G	4: 106,747,931	M497V	probably benign	Het
Fam186b	C	A	15: 99,280,128	G439V	probably benign	Het
Fank1	A	G	7: 133,876,765	Y185C	probably damaging	Het
Gart	T	C	16: 91,630,602		probably benign	Het
Glb1l	T	C	1: 75,199,720	Y572C	probably damaging	Het
Gm11563	C	T	11: 99,658,437	A164T	unknown	Het
Gnb4	C	T	3: 32,591,207	V112I	probably benign	Het
Gsdmc3	T	A	15: 63,859,693	D330V	probably damaging	Het
H2-DMa	C	T	17: 34,137,960	T144M	probably damaging	Het
Haus6	A	T	4: 86,583,514	F707I	possibly damaging	Het
Hmcn1	T	A	1: 150,594,016	T4971S	probably benign	Het
Ints6	A	T	14: 62,696,759	F766L	probably benign	Het
Kdm5d	T	A	Y: 927,330	M650K	probably damaging	Het
Kif21b	T	C	1: 136,159,428	F881S	possibly damaging	Het
Klrk1	C	A	6: 129,614,635	Q176H	possibly damaging	Het
Ky	T	C	9: 102,537,621	V244A	probably benign	Het
Mia2	T	A	12: 59,154,419	L191M	possibly damaging	Het
Miga2	T	A	2: 30,381,744		probably benign	Het
Mtss1l	C	T	8: 110,737,948	P322S	probably damaging	Het
Nalcn	A	G	14: 123,299,141		probably benign	Het
Ncbp3	T	A	11: 73,049,845		probably benign	Het
Nprl3	G	A	11: 32,234,876	L378F	probably damaging	Het
Ntrk2	A	T	13: 58,846,821	M184L	probably benign	Het
Olfr311	T	G	11: 58,841,443	C110G	probably damaging	Het
Olfr738	G	T	14: 50,413,697	C51F	probably benign	Het
Osbpl9	T	C	4: 109,083,128	E287G	probably damaging	Het
Parva	T	C	7: 112,576,411	F250L	probably damaging	Het
Pcdhb11	C	T	18: 37,421,811	Q65*	probably null	Het
Phtf1	A	G	3: 103,993,765	T377A	probably damaging	Het
Pkp4	G	A	2: 59,322,643	V612I	possibly damaging	Het
Plscr2	C	A	9: 92,287,654	S52R	probably benign	Het
Pnisr	C	T	4: 21,874,092		probably benign	Het
Pole2	A	C	12: 69,209,879	S291A	probably damaging	Het
Ppp2r5d	A	G	17: 46,684,018	F586L	probably benign	Het
Prrx1	G	A	1: 163,257,816	R182C	probably damaging	Het
Ptprs	A	G	17: 56,429,103	I110T	possibly damaging	Het
Rasgrf2	G	A	13: 91,919,817		probably benign	Het
Riox2	T	C	16: 59,491,892	V464A	probably benign	Het
Robo2	A	G	16: 73,967,802	V646A	possibly damaging	Het
Ros1	T	A	10: 52,118,348	I1279F	probably damaging	Het
Siglec1	G	A	2: 131,074,268	T1254M	probably benign	Het
Snx7	T	C	3: 117,846,675	N62D	probably damaging	Het
Sp100	G	A	1: 85,659,867		probably null	Het
Stil	A	T	4: 115,007,159	I86L	possibly damaging	Het
Tbc1d16	T	A	11: 119,209,038	D170V	probably benign	Het
Tmem2	T	A	19: 21,817,971	S743T	probably benign	Het
Trappc13	G	A	13: 104,161,081	T105M	probably damaging	Het
Trhr	T	A	15: 44,229,500	S378T	probably benign	Het
Ttc7b	T	C	12: 100,500,073		probably null	Het
Vegfc	A	T	8: 54,157,139	Y110F	probably benign	Het
Vmn1r184	C	A	7: 26,267,177	P116H	possibly damaging	Het
Vmn2r5	A	T	3: 64,503,814	C444*	probably null	Het
Zfp819	T	A	7: 43,616,444	V41E	probably benign	Het

Other mutations in Zfp341

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00499:Zfp341	APN	2	154634231	missense	probably damaging	1.00
IGL01352:Zfp341	APN	2	154628896	missense	probably benign	0.00
IGL01748:Zfp341	APN	2	154628927	missense	probably damaging	0.99
IGL02260:Zfp341	APN	2	154642049	missense	possibly damaging	0.89
IGL02329:Zfp341	APN	2	154632304	missense	possibly damaging	0.90
casanova_grimbacher	UTSW	2	154624881	missense	probably damaging	1.00
Specious	UTSW	2	154646134	missense	possibly damaging	0.63
R0079:Zfp341	UTSW	2	154624994	nonsense	probably null
R0570:Zfp341	UTSW	2	154646068	missense	probably benign	0.02
R1018:Zfp341	UTSW	2	154646052	missense	probably damaging	1.00
R1611:Zfp341	UTSW	2	154645703	missense	probably damaging	1.00
R1733:Zfp341	UTSW	2	154641378	missense	probably benign	0.19
R1822:Zfp341	UTSW	2	154646134	missense	possibly damaging	0.63
R1956:Zfp341	UTSW	2	154638212	missense	probably benign	0.09
R2437:Zfp341	UTSW	2	154628801	missense	probably damaging	0.97
R3623:Zfp341	UTSW	2	154624881	missense	probably damaging	1.00
R4417:Zfp341	UTSW	2	154628987	missense	possibly damaging	0.94
R4806:Zfp341	UTSW	2	154645866	utr 3 prime	probably benign
R4807:Zfp341	UTSW	2	154645866	utr 3 prime	probably benign
R4863:Zfp341	UTSW	2	154645866	utr 3 prime	probably benign
R4955:Zfp341	UTSW	2	154638030	missense	probably damaging	0.98
R4962:Zfp341	UTSW	2	154626814	missense	possibly damaging	0.80
R5484:Zfp341	UTSW	2	154625001	missense	probably benign	0.00
R5864:Zfp341	UTSW	2	154643554	missense	possibly damaging	0.95
R5877:Zfp341	UTSW	2	154632289	missense	probably damaging	1.00
R5975:Zfp341	UTSW	2	154630441	missense	probably damaging	1.00
R5990:Zfp341	UTSW	2	154645659	missense	probably damaging	0.98
R6057:Zfp341	UTSW	2	154625034	missense	probably benign	0.01
R6882:Zfp341	UTSW	2	154638023	missense	probably damaging	1.00
R7686:Zfp341	UTSW	2	154624861	missense	probably damaging	0.96
R7701:Zfp341	UTSW	2	154634080	splice site	probably null
R7847:Zfp341	UTSW	2	154634194	missense	probably damaging	1.00
R8109:Zfp341	UTSW	2	154627900	missense	probably benign	0.04
R9313:Zfp341	UTSW	2	154627987	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTGGGTCAGTCACTGTCCAGTACC -3'
(R):5'- TAACAGCCACCCTTGATGCTGAGC -3'

Sequencing Primer
(F):5'- TGTCCAGTACCACACAGCC -3'
(R):5'- CCTTGATGCTGAGCTGACC -3'

Posted On

2013-07-11