|Institutional Source||Beutler Lab|
|Gene Name||origin recognition complex, subunit 4|
|Synonyms||mMmORC4, Orc4, Orc4l, Orc4P|
|Is this an essential gene?||Probably essential (E-score: 0.967)|
|Stock #||R7572 (G1)|
|Chromosomal Location||48902824-48950277 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 48910236 bp|
|Amino Acid Change||Methionine to Lysine at position 300 (M300K)|
|Ref Sequence||ENSEMBL: ENSMUSP00000028098 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000028098]|
|Predicted Effect||probably benign
AA Change: M300K
PolyPhen 2 Score 0.013 (Sensitivity: 0.96; Specificity: 0.78)
AA Change: M300K
|Coding Region Coverage||
|Validation Efficiency||95% (56/59)|
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. This gene encodes a subunit of the ORC complex. Several alternatively spliced transcript variants, some of which encode the same protein, have been reported for this gene. [provided by RefSeq, Oct 2010]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Orc4||
(F):5'- TGTGGTATGGGATGCAGAACAC -3'
(R):5'- GCATCTGAACTAAGTCATTGCTAC -3'
(F):5'- TATGGGATGCAGAACACAAAGAG -3'
(R):5'- CATTTTCATGGAGCAAAGTTTAAGG -3'