Mutagenetix > Incidental Mutations

Incidental Mutation 'R7581:Taar4'

586757

Institutional Source

Beutler Lab

Gene Symbol

Taar4

Ensembl Gene

ENSMUSG00000069707

Gene Name

trace amine-associated receptor 4

Synonyms

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R7581 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

23960494-23961537 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 23961154 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Asparagine at position 221 (H221N)

Ref Sequence

ENSEMBL: ENSMUSP00000090330 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092660]

AlphaFold

Q5QD15

Predicted Effect

probably damaging
Transcript: ENSMUST00000092660
AA Change: H221N

PolyPhen 2 Score 0.972 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000090330
Gene: ENSMUSG00000069707
AA Change: H221N

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	44	328	9.2e-11	PFAM
Pfam:7tm_1	50	313	4.6e-63	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

98% (47/48)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit impaired olfactory response and aversion to PEA and puma urine. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5430403G16Rik	G	A	5: 109,690,788		probably benign	Het
Adamts9	A	C	6: 92,937,338	D196E	probably benign	Het
Adnp	A	T	2: 168,183,466	D636E	probably damaging	Het
Afdn	T	C	17: 13,849,238	V723A	probably damaging	Het
Arid1a	A	T	4: 133,680,351	F1897I	unknown	Het
Atp6v0b	T	C	4: 117,885,286	M137V	probably benign	Het
Bicd1	T	A	6: 149,519,004	I784N	probably damaging	Het
Cckbr	A	G	7: 105,433,786	T119A	probably benign	Het
Creb5	T	C	6: 53,681,237	L184P	probably damaging	Het
Cyp2a22	T	G	7: 26,938,148	K141Q	possibly damaging	Het
D16Ertd472e	T	C	16: 78,546,557	T219A	possibly damaging	Het
Ep400	A	T	5: 110,756,025	L236Q	unknown	Het
Exph5	A	G	9: 53,372,557	S313G	possibly damaging	Het
Gm10024	T	C	10: 77,711,563	V36A	unknown	Het
Gm5493	A	G	17: 22,747,274	E44G	probably damaging	Het
Gtdc1	A	T	2: 44,790,005		probably null	Het
Invs	T	C	4: 48,421,909	V847A	probably benign	Het
Kif18b	C	A	11: 102,914,722	Q236H	probably damaging	Het
Kntc1	A	G	5: 123,816,755	T2079A	probably benign	Het
Large2	A	G	2: 92,370,193	S89P	probably damaging	Het
Maml3	A	C	3: 51,856,768	D258E	probably benign	Het
Mdga2	A	T	12: 66,506,255	M868K	probably damaging	Het
Mtss1l	A	G	8: 110,726,213	E30G	possibly damaging	Het
Muc16	T	C	9: 18,645,614	I3128V	unknown	Het
Mug2	C	T	6: 122,063,711	T740I	probably damaging	Het
Noc2l	T	C	4: 156,245,449	V612A	probably benign	Het
Olfr420	G	A	1: 174,158,771		probably null	Het
Olfr979	A	T	9: 40,000,422	D268E	probably damaging	Het
Padi6	T	C	4: 140,728,929	T585A	probably benign	Het
Pcdhga1	A	T	18: 37,662,177	N78I	probably damaging	Het
Pdgfd	A	G	9: 6,293,894	Y156C	probably damaging	Het
Peg10	GCACATCAGGATCC	GCACATCAGGATCCCCATCAGGATCCTCCACATCAGGATCC	6: 4,756,452		probably benign	Het
Pi4ka	C	T	16: 17,301,060	V1307I		Het
Pip5k1c	A	G	10: 81,308,960	N212D	probably damaging	Het
Plekha1	A	G	7: 130,910,865	T264A	probably benign	Het
Polr2b	A	G	5: 77,326,704	R463G	probably damaging	Het
Psd2	T	G	18: 35,979,997	D248E	probably benign	Het
Rag1	G	T	2: 101,643,304	Q498K	possibly damaging	Het
Ryr3	A	G	2: 112,753,027	I2853T	probably damaging	Het
Selenow	G	T	7: 15,922,382		probably null	Het
Sned1	C	T	1: 93,256,545	S165F	probably benign	Het
Spata31d1a	A	G	13: 59,704,139		probably null	Het
Tgm6	A	T	2: 130,141,285	R265W	probably damaging	Het
Trpm1	A	T	7: 64,204,555	Q275L	probably benign	Het
Ulk3	T	C	9: 57,592,042	L173P	probably damaging	Het
Urah	A	T	7: 140,835,627	T3S	probably benign	Het
Vmn1r149	A	T	7: 22,437,904	V109D	probably damaging	Het
Xpc	A	T	6: 91,498,017		probably benign	Het
Zglp1	T	A	9: 21,062,708	K227N	probably damaging	Het

Other mutations in Taar4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02576:Taar4	APN	10	23961011	missense	probably damaging	1.00
IGL03202:Taar4	APN	10	23960794	missense	probably damaging	1.00
I2288:Taar4	UTSW	10	23960920	missense	probably benign	0.03
R0103:Taar4	UTSW	10	23961406	missense	probably damaging	1.00
R0103:Taar4	UTSW	10	23961406	missense	probably damaging	1.00
R0514:Taar4	UTSW	10	23960882	missense	probably damaging	1.00
R1222:Taar4	UTSW	10	23961332	missense	probably benign	0.05
R1248:Taar4	UTSW	10	23961038	missense	possibly damaging	0.95
R1514:Taar4	UTSW	10	23960612	missense	possibly damaging	0.71
R1921:Taar4	UTSW	10	23961341	missense	probably damaging	1.00
R2074:Taar4	UTSW	10	23961173	missense	probably benign	0.18
R2354:Taar4	UTSW	10	23961014	missense	probably damaging	1.00
R2392:Taar4	UTSW	10	23961274	missense	possibly damaging	0.94
R2698:Taar4	UTSW	10	23961430	missense	probably damaging	1.00
R3902:Taar4	UTSW	10	23961015	missense	probably damaging	1.00
R4688:Taar4	UTSW	10	23960833	missense	probably damaging	1.00
R5495:Taar4	UTSW	10	23961283	missense	possibly damaging	0.95
R5595:Taar4	UTSW	10	23960741	missense	probably damaging	1.00
R5773:Taar4	UTSW	10	23961158	missense	probably damaging	1.00
R7403:Taar4	UTSW	10	23961059	missense	probably damaging	1.00
R7736:Taar4	UTSW	10	23960999	missense	probably damaging	1.00
R7859:Taar4	UTSW	10	23961134	missense	probably benign	0.35
R8676:Taar4	UTSW	10	23960903	missense	possibly damaging	0.56

Predicted Primers

PCR Primer
(F):5'- GGTAGGGGTCTTTCTACTCATC -3'
(R):5'- AGGGTCTGTGATTGTCAAGAC -3'

Sequencing Primer
(F):5'- AGGGGTCTTTCTACTCATCAGTTG -3'
(R):5'- AAAGTCTTGGTGGCCTTG -3'

Posted On

2019-10-24