Mutagenetix > Incidental Mutation

Incidental Mutation 'R7583:Helz2'

586858

Institutional Source

Beutler Lab

Gene Symbol

Helz2

Ensembl Gene

ENSMUSG00000027580

Gene Name

helicase with zinc finger 2, transcriptional coactivator

Synonyms

BC006779

MMRRC Submission

045666-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7583 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

181227615-181242027 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 181237572 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 751 (H751L)

Ref Sequence

ENSEMBL: ENSMUSP00000091756 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094203] [ENSMUST00000108831] [ENSMUST00000121484]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000094203
AA Change: H751L

PolyPhen 2 Score 0.061 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000091756
Gene: ENSMUSG00000027580
AA Change: H751L

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000108831
AA Change: H751L

PolyPhen 2 Score 0.057 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000104459
Gene: ENSMUSG00000027580
AA Change: H751L

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000121484
AA Change: H751L

PolyPhen 2 Score 0.074 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000112917
Gene: ENSMUSG00000027580
AA Change: H751L

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
Pfam:AAA_11	761	877	3.9e-10	PFAM
Pfam:AAA_19	780	849	1.7e-7	PFAM
Pfam:AAA_11	870	952	2e-15	PFAM
Pfam:AAA_12	958	1162	3.8e-26	PFAM
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
Pfam:AAA_11	2400	2653	4e-42	PFAM
Pfam:AAA_12	2660	2866	2e-47	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

99% (95/96)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a nuclear transcriptional co-activator for peroxisome proliferator activated receptor alpha. The encoded protein contains a zinc finger and is a helicase that appears to be part of the peroxisome proliferator activated receptor alpha interacting complex. This gene is a member of the DNA2/NAM7 helicase gene family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit slower weight gain, hyperleptinemia, increased oxygen consumption, decreased respiratory quotient, decreased liver triglyceride level and ameliorated hyperlipidemia and hepatosteatosis when fed a high-fat diet. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 98 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932414N04Rik	A	T	2: 68,739,326	D462V	probably damaging	Het
Abcc1	T	C	16: 14,404,038	W222R	probably damaging	Het
Adamts13	A	G	2: 26,973,953	K48E	probably benign	Het
Adgrd1	A	C	5: 129,179,588	T674P	probably benign	Het
Agbl4	A	T	4: 111,118,953	Y169F	possibly damaging	Het
Ahnak	T	A	19: 9,006,093	N1580K	possibly damaging	Het
Angptl8	A	C	9: 21,835,914		probably null	Het
Ap3d1	G	A	10: 80,709,458	T1053I	probably benign	Het
Atad2	T	C	15: 58,126,664	T139A	probably benign	Het
Bdp1	T	A	13: 100,049,812	T1711S	probably damaging	Het
Bod1l	T	C	5: 41,833,790	I141V	probably damaging	Het
Cavin2	A	T	1: 51,289,618	N78I	possibly damaging	Het
Ccnb1	T	C	13: 100,779,754	H406R	probably benign	Het
Cd200r4	A	G	16: 44,833,421	T194A	probably damaging	Het
Cdt1	G	A	8: 122,570,256	R263H	probably damaging	Het
Cep85l	A	T	10: 53,281,354	I751N	probably damaging	Het
Clasp2	A	G	9: 113,908,687	D1043G	probably benign	Het
Cmip	A	T	8: 117,454,952	N578I	probably damaging	Het
Col2a1	T	G	15: 97,976,184	K1440N	unknown	Het
Cpne2	A	T	8: 94,555,581	M252L	probably benign	Het
Crb2	A	G	2: 37,790,595	T512A	probably benign	Het
Csmd1	A	G	8: 15,998,833	Y2290H	probably damaging	Het
Ctnnd1	A	T	2: 84,612,061	D642E	probably damaging	Het
Ctns	A	G	11: 73,188,470	S141P	probably benign	Het
Cwh43	A	G	5: 73,434,289	Q575R	probably benign	Het
Cyp2b19	C	A	7: 26,759,064	T68K	probably damaging	Het
Dcaf6	A	G	1: 165,333,310	S849P	probably damaging	Het
Dnm3	T	A	1: 162,477,774	Q17L	possibly damaging	Het
Enpp3	A	T	10: 24,836,092	M1K	probably null	Het
Fam124a	T	A	14: 62,606,559	C505*	probably null	Het
Fam160b1	T	A	19: 57,378,602	D192E	probably benign	Het
Fsip2	A	G	2: 82,975,241	I635V	probably benign	Het
Gm5878	A	T	6: 85,118,700		probably null	Het
Gm6205	A	T	5: 94,682,894	T84S	possibly damaging	Het
Gpld1	C	A	13: 24,975,760	A437D	probably damaging	Het
Grk5	G	T	19: 61,083,204	V401L	possibly damaging	Het
Gucy2g	A	T	19: 55,235,615	L259Q	probably damaging	Het
Habp2	T	A	19: 56,311,804	D191E	probably benign	Het
Hgsnat	A	G	8: 25,971,564		probably null	Het
Hivep1	T	A	13: 42,164,240	V2064E	probably damaging	Het
Ikbke	C	T	1: 131,276,479	A26T	probably damaging	Het
Ivd	C	A	2: 118,862,131	D37E	probably damaging	Het
Kif26b	G	A	1: 178,530,445	W40*	probably null	Het
Klrc2	A	G	6: 129,659,311	S114P	probably damaging	Het
Lama1	A	T	17: 67,761,621	T772S		Het
Lamc1	T	A	1: 153,243,232	K880N	possibly damaging	Het
Lmf1	G	A	17: 25,655,449	D12N		Het
Lpin2	G	T	17: 71,231,396	E384*	probably null	Het
Lrrc23	T	C	6: 124,779,578		probably benign	Het
Lrrc31	T	C	3: 30,691,099		probably null	Het
Luc7l2	A	G	6: 38,551,885	Q13R	probably damaging	Het
Ly6c1	G	T	15: 75,048,497	H5Q	probably damaging	Het
Lyst	A	T	13: 13,635,887	H714L	probably damaging	Het
Man2a2	C	T	7: 80,366,944	R374H	probably damaging	Het
Mchr1	A	T	15: 81,237,441	T131S	probably benign	Het
Msto1	T	C	3: 88,912,929		probably null	Het
Myh1	C	T	11: 67,220,913	T1698I	probably benign	Het
Ncapd3	G	T	9: 27,071,848	C964F	probably damaging	Het
Nefh	T	C	11: 4,941,089	E510G	probably damaging	Het
Olfr1246	A	T	2: 89,590,751	Y121*	probably null	Het
Olfr1513	T	C	14: 52,349,903	T48A	possibly damaging	Het
Olfr357	A	C	2: 36,997,080	K90T	probably damaging	Het
Olfr362	A	T	2: 37,105,527	L41*	probably null	Het
Olfr538	A	G	7: 140,574,210	E19G	probably benign	Het
P4ha1	T	A	10: 59,369,640	S497R	probably benign	Het
Palb2	T	C	7: 122,127,342	D435G	probably benign	Het
Papola	C	T	12: 105,811,045	P282L	probably damaging	Het
Pcdhgb1	A	G	18: 37,682,324	R623G	probably damaging	Het
Pcolce	T	G	5: 137,607,445	K229Q	probably benign	Het
Pkhd1l1	T	C	15: 44,568,364		probably null	Het
Ppp6r3	T	A	19: 3,490,790	T443S	probably benign	Het
Psg20	T	A	7: 18,682,483	D236V	probably damaging	Het
Ptgr2	T	G	12: 84,308,405	S304R	probably damaging	Het
Ptpn22	A	T	3: 103,902,114	D681V	probably benign	Het
Rab3gap1	T	C	1: 127,930,875	S574P	probably benign	Het
Slc36a1	A	G	11: 55,213,928		probably null	Het
Slc9a5	A	G	8: 105,363,272	S621G	possibly damaging	Het
Sntg1	A	G	1: 8,445,025		probably null	Het
Snx17	A	G	5: 31,196,533	N222D	possibly damaging	Het
Spata19	G	T	9: 27,400,433	S116I	possibly damaging	Het
Spn	G	A	7: 127,137,062	A91V	probably damaging	Het
Srrm3	T	C	5: 135,852,281	V145A	probably benign	Het
St7	A	T	6: 17,942,754	T575S	possibly damaging	Het
Taf2	A	T	15: 55,064,676	Y110*	probably null	Het
Tcf20	T	C	15: 82,855,276	E658G	possibly damaging	Het
Tdrd6	G	T	17: 43,624,238	A1973E	probably benign	Het
Tg	T	G	15: 66,764,418	L2237W	probably damaging	Het
Ticrr	C	A	7: 79,696,739	Y1882*	probably null	Het
Trim45	T	A	3: 100,925,023	C191S	probably damaging	Het
Upf3a	G	A	8: 13,785,889		probably null	Het
Vmn2r42	A	T	7: 8,194,741	L293*	probably null	Het
Vmn2r62	T	A	7: 42,788,042	Q339H	possibly damaging	Het
Wdr24	A	T	17: 25,825,830	R220W	probably null	Het
Wdr5	T	A	2: 27,518,775	S22T	probably benign	Het
Zfp37	G	A	4: 62,192,016		probably benign	Het
Zfp735	A	T	11: 73,711,107	L292F	possibly damaging	Het
Zfp985	T	A	4: 147,583,489	C271*	probably null	Het
Zranb1	G	A	7: 132,983,896	R691Q	probably benign	Het

Other mutations in Helz2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Helz2	APN	2	181229702	missense	probably damaging	1.00
IGL00515:Helz2	APN	2	181233006	nonsense	probably null
IGL00704:Helz2	APN	2	181234385	missense	probably damaging	1.00
IGL00847:Helz2	APN	2	181232245	missense	possibly damaging	0.73
IGL01448:Helz2	APN	2	181233977	missense	probably damaging	1.00
IGL01783:Helz2	APN	2	181232881	missense	probably damaging	1.00
IGL01790:Helz2	APN	2	181238481	missense	probably benign	0.29
IGL02116:Helz2	APN	2	181232185	missense	probably damaging	1.00
IGL02226:Helz2	APN	2	181231690	missense	probably damaging	1.00
IGL02402:Helz2	APN	2	181230911	missense	probably damaging	1.00
IGL02403:Helz2	APN	2	181231022	missense	probably damaging	1.00
IGL02733:Helz2	APN	2	181235026	missense	probably benign	0.14
IGL02869:Helz2	APN	2	181231146	intron	probably benign
IGL03003:Helz2	APN	2	181240253	missense	probably damaging	1.00
IGL03060:Helz2	APN	2	181229222	critical splice donor site	probably null
IGL03310:Helz2	APN	2	181231804	missense	probably benign	0.00
Colby	UTSW	2	181233202	missense	probably damaging	1.00
ANU74:Helz2	UTSW	2	181234834	missense	probably benign	0.03
R0013:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0013:Helz2	UTSW	2	181240959	missense	probably benign
R0014:Helz2	UTSW	2	181240511	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	181240511	missense	probably damaging	1.00
R0016:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0018:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0055:Helz2	UTSW	2	181228821	missense	possibly damaging	0.47
R0055:Helz2	UTSW	2	181228821	missense	possibly damaging	0.47
R0071:Helz2	UTSW	2	181236407	missense	probably damaging	1.00
R0071:Helz2	UTSW	2	181236407	missense	probably damaging	1.00
R0111:Helz2	UTSW	2	181237802	missense	probably benign	0.30
R0117:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0135:Helz2	UTSW	2	181232269	missense	probably damaging	1.00
R0194:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	181230430	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	181230430	missense	probably damaging	1.00
R0254:Helz2	UTSW	2	181232759	missense	probably damaging	1.00
R0410:Helz2	UTSW	2	181230593	missense	probably damaging	1.00
R0442:Helz2	UTSW	2	181232209	missense	probably damaging	0.97
R0497:Helz2	UTSW	2	181229656	missense	probably damaging	0.97
R0517:Helz2	UTSW	2	181227770	missense	probably benign	0.00
R0541:Helz2	UTSW	2	181234825	missense	possibly damaging	0.89
R0542:Helz2	UTSW	2	181232089	missense	probably damaging	1.00
R0591:Helz2	UTSW	2	181232116	missense	probably damaging	0.96
R0692:Helz2	UTSW	2	181240881	missense	probably benign
R0826:Helz2	UTSW	2	181240853	missense	possibly damaging	0.51
R0834:Helz2	UTSW	2	181230777	missense	probably damaging	1.00
R0880:Helz2	UTSW	2	181236135	missense	probably benign
R1170:Helz2	UTSW	2	181229815	missense	probably damaging	1.00
R1186:Helz2	UTSW	2	181231128	missense	probably damaging	1.00
R1344:Helz2	UTSW	2	181237596	missense	possibly damaging	0.89
R1358:Helz2	UTSW	2	181232981	missense	probably damaging	1.00
R1436:Helz2	UTSW	2	181235524	missense	probably damaging	0.99
R1464:Helz2	UTSW	2	181239654	missense	probably damaging	1.00
R1464:Helz2	UTSW	2	181239654	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	181236297	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	181236297	missense	probably damaging	1.00
R1477:Helz2	UTSW	2	181232804	missense	probably benign	0.00
R1564:Helz2	UTSW	2	181233228	missense	probably benign	0.01
R1584:Helz2	UTSW	2	181236297	missense	probably damaging	1.00
R1655:Helz2	UTSW	2	181234147	missense	probably damaging	0.99
R1757:Helz2	UTSW	2	181236263	missense	probably damaging	1.00
R1779:Helz2	UTSW	2	181234987	missense	probably benign
R1779:Helz2	UTSW	2	181238459	missense	possibly damaging	0.84
R1837:Helz2	UTSW	2	181229289	missense	probably damaging	1.00
R1845:Helz2	UTSW	2	181232085	missense	probably benign	0.02
R1894:Helz2	UTSW	2	181234289	missense	probably damaging	1.00
R1913:Helz2	UTSW	2	181233750	missense	probably damaging	1.00
R2005:Helz2	UTSW	2	181231329	missense	probably benign	0.45
R2034:Helz2	UTSW	2	181232578	missense	probably damaging	1.00
R2036:Helz2	UTSW	2	181237479	missense	probably benign	0.03
R2061:Helz2	UTSW	2	181240544	missense	probably damaging	1.00
R2088:Helz2	UTSW	2	181235102	missense	probably benign	0.07
R2142:Helz2	UTSW	2	181231380	missense	probably benign
R2180:Helz2	UTSW	2	181233732	missense	probably damaging	1.00
R2192:Helz2	UTSW	2	181229048	nonsense	probably null
R2248:Helz2	UTSW	2	181233433	missense	probably benign	0.33
R2495:Helz2	UTSW	2	181232912	missense	probably damaging	0.99
R2886:Helz2	UTSW	2	181240742	missense	probably benign
R3617:Helz2	UTSW	2	181233061	missense	probably damaging	1.00
R3776:Helz2	UTSW	2	181240389	nonsense	probably null
R3803:Helz2	UTSW	2	181239996	missense	probably damaging	0.96
R4043:Helz2	UTSW	2	181229710	missense	probably benign	0.00
R4052:Helz2	UTSW	2	181240475	missense	probably damaging	1.00
R4232:Helz2	UTSW	2	181229902	missense	probably damaging	1.00
R4521:Helz2	UTSW	2	181228833	missense	probably benign
R4624:Helz2	UTSW	2	181239308	missense	probably damaging	0.99
R4720:Helz2	UTSW	2	181238417	missense	probably damaging	1.00
R4831:Helz2	UTSW	2	181237417	missense	probably damaging	1.00
R4852:Helz2	UTSW	2	181230120	missense	probably damaging	1.00
R4894:Helz2	UTSW	2	181236147	missense	probably benign	0.01
R4915:Helz2	UTSW	2	181232438	missense	possibly damaging	0.80
R4965:Helz2	UTSW	2	181240916	missense	possibly damaging	0.79
R5022:Helz2	UTSW	2	181240569	missense	probably benign
R5089:Helz2	UTSW	2	181235149	missense	probably benign	0.14
R5190:Helz2	UTSW	2	181230757	critical splice donor site	probably null
R5309:Helz2	UTSW	2	181234846	missense	probably benign	0.08
R5358:Helz2	UTSW	2	181235528	missense	probably damaging	1.00
R5379:Helz2	UTSW	2	181235069	missense	probably benign
R5559:Helz2	UTSW	2	181230126	missense	probably damaging	0.98
R5591:Helz2	UTSW	2	181240258	missense	probably damaging	0.99
R5596:Helz2	UTSW	2	181237289	intron	probably benign
R5805:Helz2	UTSW	2	181240508	missense	probably damaging	1.00
R5823:Helz2	UTSW	2	181236396	missense	possibly damaging	0.92
R5825:Helz2	UTSW	2	181232656	missense	probably benign	0.02
R5873:Helz2	UTSW	2	181234028	missense	possibly damaging	0.78
R5928:Helz2	UTSW	2	181230384	missense	possibly damaging	0.82
R5936:Helz2	UTSW	2	181230767	missense	probably damaging	1.00
R5975:Helz2	UTSW	2	181231050	missense	probably benign	0.08
R6045:Helz2	UTSW	2	181240313	missense	probably benign	0.03
R6077:Helz2	UTSW	2	181233038	missense	probably benign	0.41
R6218:Helz2	UTSW	2	181232294	missense	probably benign	0.03
R6218:Helz2	UTSW	2	181235945	missense	probably damaging	1.00
R6315:Helz2	UTSW	2	181233202	missense	probably damaging	1.00
R6346:Helz2	UTSW	2	181233467	missense	probably damaging	1.00
R6371:Helz2	UTSW	2	181233467	missense	probably damaging	1.00
R6372:Helz2	UTSW	2	181233467	missense	probably damaging	1.00
R6373:Helz2	UTSW	2	181233467	missense	probably damaging	1.00
R6385:Helz2	UTSW	2	181233467	missense	probably damaging	1.00
R6464:Helz2	UTSW	2	181235069	missense	probably benign
R6581:Helz2	UTSW	2	181229379	missense	probably damaging	0.99
R6651:Helz2	UTSW	2	181239557	nonsense	probably null
R6964:Helz2	UTSW	2	181230428	missense	probably damaging	1.00
R7061:Helz2	UTSW	2	181240514	missense	probably damaging	1.00
R7153:Helz2	UTSW	2	181231285	missense	probably benign	0.00
R7372:Helz2	UTSW	2	181238423	missense	possibly damaging	0.61
R7512:Helz2	UTSW	2	181230854	missense	probably benign	0.00
R7512:Helz2	UTSW	2	181235600	splice site	probably null
R7724:Helz2	UTSW	2	181231996	missense	probably damaging	1.00
R7733:Helz2	UTSW	2	181230355	missense	possibly damaging	0.63
R7748:Helz2	UTSW	2	181234531	missense	probably damaging	1.00
R7774:Helz2	UTSW	2	181233991	missense	probably benign
R7799:Helz2	UTSW	2	181237989	missense	probably benign	0.15
R7841:Helz2	UTSW	2	181232902	missense	probably damaging	1.00
R7939:Helz2	UTSW	2	181237750	missense	probably damaging	0.99
R8026:Helz2	UTSW	2	181240205	missense	probably benign	0.34
R8030:Helz2	UTSW	2	181237896	missense	possibly damaging	0.55
R8080:Helz2	UTSW	2	181238262	missense	probably damaging	0.99
R8237:Helz2	UTSW	2	181229331	missense	possibly damaging	0.65
R8245:Helz2	UTSW	2	181238102	missense	probably damaging	1.00
R8304:Helz2	UTSW	2	181230157	missense	probably benign	0.03
R8486:Helz2	UTSW	2	181229331	missense	probably damaging	1.00
R8556:Helz2	UTSW	2	181229557	missense	probably damaging	1.00
R8878:Helz2	UTSW	2	181232767	missense	possibly damaging	0.67
R8907:Helz2	UTSW	2	181233127	missense	possibly damaging	0.47
R8911:Helz2	UTSW	2	181238380	missense
R8953:Helz2	UTSW	2	181233091	missense	probably damaging	1.00
R8963:Helz2	UTSW	2	181229614	missense	probably damaging	1.00
R8969:Helz2	UTSW	2	181237788	missense	probably benign	0.19
R8976:Helz2	UTSW	2	181234693	missense	possibly damaging	0.46
R9015:Helz2	UTSW	2	181228999	missense	probably damaging	1.00
R9031:Helz2	UTSW	2	181232468	missense	possibly damaging	0.78
R9052:Helz2	UTSW	2	181240175	missense	possibly damaging	0.78
R9089:Helz2	UTSW	2	181239640	missense	probably damaging	1.00
R9145:Helz2	UTSW	2	181240055	missense	probably damaging	1.00
R9185:Helz2	UTSW	2	181230090	missense	probably benign
R9186:Helz2	UTSW	2	181234664	missense	possibly damaging	0.57
R9373:Helz2	UTSW	2	181240948	missense	probably benign
R9407:Helz2	UTSW	2	181240182	missense	probably benign	0.01
R9465:Helz2	UTSW	2	181232917	missense	probably benign	0.01
R9502:Helz2	UTSW	2	181236452	missense	possibly damaging	0.47
R9538:Helz2	UTSW	2	181240221	missense	probably damaging	1.00
R9554:Helz2	UTSW	2	181240677	missense	probably damaging	0.96
R9659:Helz2	UTSW	2	181240232	missense	probably benign	0.00
R9800:Helz2	UTSW	2	181240823	missense	probably damaging	0.99
X0064:Helz2	UTSW	2	181231741	missense	probably damaging	1.00
Z1176:Helz2	UTSW	2	181237564	missense	probably benign	0.39
Z1177:Helz2	UTSW	2	181235961	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTGCTGGACAACCTCAAGTG -3'
(R):5'- ACAGTATACCAAGTGCGGCTG -3'

Sequencing Primer
(F):5'- AACCTCAAGTGCGGCCATG -3'
(R):5'- AGTTCAGACCATGCGCTGTG -3'

Posted On

2019-10-24