Mutagenetix > Incidental Mutation

Incidental Mutation 'R7587:Olfr668'

587212

Institutional Source

Beutler Lab

Gene Symbol

Olfr668

Ensembl Gene

ENSMUSG00000057770

Gene Name

olfactory receptor 668

Synonyms

GA_x6K02T2PBJ9-7554614-7553658, MOR34-3

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.183) question?

Stock #

R7587 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

104922291-104928057 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 104925056 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Histidine at position 236 (R236H)

Ref Sequence

ENSEMBL: ENSMUSP00000150824 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164391] [ENSMUST00000215359] [ENSMUST00000217177]

AlphaFold

Q8VGW3

Predicted Effect

probably benign
Transcript: ENSMUST00000164391
AA Change: R236H

PolyPhen 2 Score 0.279 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000130975
Gene: ENSMUSG00000057770
AA Change: R236H

Domain	Start	End	E-Value	Type
Pfam:7tm_4	33	313	7.2e-103	PFAM
Pfam:7TM_GPCR_Srsx	37	210	4.7e-10	PFAM
Pfam:7tm_1	43	295	4.6e-18	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215359
AA Change: R236H

PolyPhen 2 Score 0.279 (Sensitivity: 0.91; Specificity: 0.88)

Predicted Effect

probably benign
Transcript: ENSMUST00000217177

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.5%

Validation Efficiency

100% (63/63)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agl	T	C	3: 116,792,087	T131A	probably damaging	Het
Asah1	A	T	8: 41,374,541	V15D	probably benign	Het
Asic1	A	C	15: 99,695,590	Q276P	probably damaging	Het
Atl2	A	T	17: 79,865,067	V158E	probably benign	Het
Atrn	T	A	2: 130,980,114	I909N	probably damaging	Het
BC034090	A	G	1: 155,217,486	V742A	probably damaging	Het
Capzb	T	A	4: 139,262,023	D85E	possibly damaging	Het
Cdh20	T	C	1: 104,941,279	F165S	probably damaging	Het
Cfap44	A	G	16: 44,404,106	E59G	probably benign	Het
Clpsl2	G	A	17: 28,549,541	V10I	probably benign	Het
D630003M21Rik	T	C	2: 158,196,388	Y1046C	probably benign	Het
D630003M21Rik	T	A	2: 158,201,056	S855C	probably damaging	Het
D6Wsu163e	A	G	6: 126,955,896	I361V	probably benign	Het
Dchs2	A	G	3: 83,304,515	I1874V	probably benign	Het
Dennd2a	T	G	6: 39,483,135	K679Q	probably damaging	Het
Dio2	A	G	12: 90,729,560	V218A	probably benign	Het
Fam208b	T	C	13: 3,568,849	K2251E	possibly damaging	Het
Gm3286	A	G	5: 95,521,411	T100A	probably damaging	Het
Gpr107	A	T	2: 31,168,826	K109N	probably benign	Het
Gpr108	C	T	17: 57,236,732	R448Q	probably damaging	Het
Gucy2c	A	G	6: 136,704,290	V932A	probably damaging	Het
Kcnj8	T	C	6: 142,566,339	T181A	probably damaging	Het
Kdm3b	A	G	18: 34,797,027		probably null	Het
Lipc	A	G	9: 70,818,924	Y168H	probably damaging	Het
Lipi	C	T	16: 75,550,215	V439M	probably benign	Het
Lpp	A	T	16: 24,762,279		probably null	Het
Lrp1b	T	G	2: 40,730,717	D3583A		Het
Ltbr	T	C	6: 125,312,352	T165A	probably benign	Het
Mroh3	A	G	1: 136,190,998	I527T	probably benign	Het
Mylk	A	G	16: 34,922,517	E1133G	probably benign	Het
Nat3	T	C	8: 67,547,574	I35T	probably damaging	Het
Ncbp3	T	C	11: 73,066,765		probably null	Het
Nedd1	G	A	10: 92,698,730	T306M	probably benign	Het
Nexn	T	C	3: 152,247,178	R316G	probably benign	Het
Nox4	A	G	7: 87,317,302	H207R	probably damaging	Het
Nsun6	T	C	2: 15,039,825	Q110R	probably benign	Het
Olfr22-ps1	C	T	11: 73,955,184	Q165*	probably null	Het
Olfr851	T	A	9: 19,497,522	V258E	probably damaging	Het
Pappa2	T	C	1: 158,851,131	D905G	probably damaging	Het
Pepd	T	C	7: 34,969,540	L195S	probably damaging	Het
Pms1	A	G	1: 53,207,316	S355P	probably benign	Het
Pop1	A	T	15: 34,502,413	K82M	probably damaging	Het
Ppp1r9b	T	A	11: 95,001,940	D655E	possibly damaging	Het
Ppt2	A	G	17: 34,626,803		probably null	Het
Prss35	T	A	9: 86,755,374	C66S	probably damaging	Het
Rfc3	A	T	5: 151,651,151	M1K	probably null	Het
Rffl	T	C	11: 82,810,148	D284G	probably damaging	Het
Robo3	T	C	9: 37,429,646	D110G	probably damaging	Het
Rtel1	G	A	2: 181,322,315	V36M	probably damaging	Het
Slc1a7	T	A	4: 108,010,486	I457K	possibly damaging	Het
Smco1	A	G	16: 32,273,723	M71V	probably benign	Het
Snrnp200	G	A	2: 127,227,902	S989N	probably damaging	Het
Spef2	T	A	15: 9,713,219	I356F	probably damaging	Het
Stk24	C	T	14: 121,302,287	A166T	probably damaging	Het
Tada2b	T	C	5: 36,476,767	I156V	probably benign	Het
Tbce	C	T	13: 14,019,742	V111M	probably damaging	Het
Tlnrd1	A	G	7: 83,882,947	L92P	probably damaging	Het
Tnr	T	A	1: 159,886,208	D735E	probably benign	Het
Tram1	T	C	1: 13,579,547	H110R	probably damaging	Het
Ttll3	CAAAGTAA	CAAAGTAAAGTAA	6: 113,399,157		probably null	Het
Unc5b	C	T	10: 60,783,120	C81Y	probably damaging	Het
Vps13a	G	A	19: 16,703,789	T1041M	probably benign	Het

Other mutations in Olfr668

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01664:Olfr668	APN	7	104925104	missense	probably damaging	1.00
IGL02250:Olfr668	APN	7	104925015	missense	probably damaging	1.00
IGL02743:Olfr668	APN	7	104924868	missense	probably damaging	0.98
IGL03105:Olfr668	APN	7	104925764	unclassified	probably benign
IGL03252:Olfr668	APN	7	104925387	missense	probably benign	0.16
IGL03387:Olfr668	APN	7	104925373	missense	probably benign	0.01
R1534:Olfr668	UTSW	7	104925414	missense	possibly damaging	0.95
R2509:Olfr668	UTSW	7	104925687	missense	probably benign	0.40
R2510:Olfr668	UTSW	7	104925687	missense	probably benign	0.40
R4739:Olfr668	UTSW	7	104924810	missense	possibly damaging	0.91
R4995:Olfr668	UTSW	7	104925735	missense	probably benign	0.01
R5071:Olfr668	UTSW	7	104925493	missense	probably benign
R5074:Olfr668	UTSW	7	104925493	missense	probably benign
R5208:Olfr668	UTSW	7	104925726	missense	probably benign
R5293:Olfr668	UTSW	7	104925279	missense	probably benign	0.00
R6061:Olfr668	UTSW	7	104925392	missense	probably benign	0.28
R6063:Olfr668	UTSW	7	104925392	missense	probably benign	0.28
R6064:Olfr668	UTSW	7	104925392	missense	probably benign	0.28
R6172:Olfr668	UTSW	7	104925296	missense	probably benign	0.40
R6492:Olfr668	UTSW	7	104925645	missense	possibly damaging	0.60
R6933:Olfr668	UTSW	7	104925123	missense	probably benign	0.21
R7040:Olfr668	UTSW	7	104925510	missense	probably benign	0.02
R7841:Olfr668	UTSW	7	104924859	missense	possibly damaging	0.59
R7869:Olfr668	UTSW	7	104925104	missense	probably damaging	1.00
R7902:Olfr668	UTSW	7	104925350	missense	probably damaging	1.00
R8296:Olfr668	UTSW	7	104925621	missense	probably benign	0.00
R8670:Olfr668	UTSW	7	104925212	missense	probably damaging	0.98
R8680:Olfr668	UTSW	7	104925413	missense	probably damaging	1.00
R8695:Olfr668	UTSW	7	104924939	missense	probably benign	0.30
R9158:Olfr668	UTSW	7	104924879	missense	probably damaging	1.00
R9261:Olfr668	UTSW	7	104925098	missense	probably benign	0.04
R9681:Olfr668	UTSW	7	104924868	missense	probably damaging	0.98
Z1177:Olfr668	UTSW	7	104924993	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTGCTTGGTCTTGACTCCATAAAC -3'
(R):5'- GCGCTTGCCCTATTGTAGAAG -3'

Sequencing Primer
(F):5'- GGTCTTGACTCCATAAACAATTGGG -3'
(R):5'- GCTTGCCCTATTGTAGAAGCATTC -3'

Posted On

2019-10-24