Mutagenetix > Incidental Mutation

Incidental Mutation 'R7587:Prss35'

587218

Institutional Source

Beutler Lab

Gene Symbol

Prss35

Ensembl Gene

ENSMUSG00000033491

Gene Name

protease, serine 35

Synonyms

6030424L22Rik

MMRRC Submission

045712-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.107) question?

Stock #

R7587 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

86743649-86758443 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 86755374 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 66 (C66S)

Ref Sequence

ENSEMBL: ENSMUSP00000035271 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000036426] [ENSMUST00000179574]

AlphaFold

Q8C0F9

Predicted Effect

probably damaging
Transcript: ENSMUST00000036426
AA Change: C66S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000035271
Gene: ENSMUSG00000033491
AA Change: C66S

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	68	83	N/A	INTRINSIC
Tryp_SPc	132	399	4.13e-2	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000179574
AA Change: C66S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000137445
Gene: ENSMUSG00000033491
AA Change: C66S

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	68	83	N/A	INTRINSIC
Tryp_SPc	132	399	4.13e-2	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.5%

Validation Efficiency

100% (63/63)

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agl	T	C	3: 116,792,087 (GRCm38)	T131A	probably damaging	Het
Asah1	A	T	8: 41,374,541 (GRCm38)	V15D	probably benign	Het
Asic1	A	C	15: 99,695,590 (GRCm38)	Q276P	probably damaging	Het
Atl2	A	T	17: 79,865,067 (GRCm38)	V158E	probably benign	Het
Atrn	T	A	2: 130,980,114 (GRCm38)	I909N	probably damaging	Het
BC034090	A	G	1: 155,217,486 (GRCm38)	V742A	probably damaging	Het
Capzb	T	A	4: 139,262,023 (GRCm38)	D85E	possibly damaging	Het
Cdh20	T	C	1: 104,941,279 (GRCm38)	F165S	probably damaging	Het
Cfap44	A	G	16: 44,404,106 (GRCm38)	E59G	probably benign	Het
Clpsl2	G	A	17: 28,549,541 (GRCm38)	V10I	probably benign	Het
D630003M21Rik	T	A	2: 158,201,056 (GRCm38)	S855C	probably damaging	Het
D630003M21Rik	T	C	2: 158,196,388 (GRCm38)	Y1046C	probably benign	Het
D6Wsu163e	A	G	6: 126,955,896 (GRCm38)	I361V	probably benign	Het
Dchs2	A	G	3: 83,304,515 (GRCm38)	I1874V	probably benign	Het
Dennd2a	T	G	6: 39,483,135 (GRCm38)	K679Q	probably damaging	Het
Dio2	A	G	12: 90,729,560 (GRCm38)	V218A	probably benign	Het
Fam208b	T	C	13: 3,568,849 (GRCm38)	K2251E	possibly damaging	Het
Gm3286	A	G	5: 95,521,411 (GRCm38)	T100A	probably damaging	Het
Gpr107	A	T	2: 31,168,826 (GRCm38)	K109N	probably benign	Het
Gpr108	C	T	17: 57,236,732 (GRCm38)	R448Q	probably damaging	Het
Gucy2c	A	G	6: 136,704,290 (GRCm38)	V932A	probably damaging	Het
Kcnj8	T	C	6: 142,566,339 (GRCm38)	T181A	probably damaging	Het
Kdm3b	A	G	18: 34,797,027 (GRCm38)		probably null	Het
Lipc	A	G	9: 70,818,924 (GRCm38)	Y168H	probably damaging	Het
Lipi	C	T	16: 75,550,215 (GRCm38)	V439M	probably benign	Het
Lpp	A	T	16: 24,762,279 (GRCm38)		probably null	Het
Lrp1b	T	G	2: 40,730,717 (GRCm38)	D3583A		Het
Ltbr	T	C	6: 125,312,352 (GRCm38)	T165A	probably benign	Het
Mroh3	A	G	1: 136,190,998 (GRCm38)	I527T	probably benign	Het
Mylk	A	G	16: 34,922,517 (GRCm38)	E1133G	probably benign	Het
Nat3	T	C	8: 67,547,574 (GRCm38)	I35T	probably damaging	Het
Ncbp3	T	C	11: 73,066,765 (GRCm38)		probably null	Het
Nedd1	G	A	10: 92,698,730 (GRCm38)	T306M	probably benign	Het
Nexn	T	C	3: 152,247,178 (GRCm38)	R316G	probably benign	Het
Nox4	A	G	7: 87,317,302 (GRCm38)	H207R	probably damaging	Het
Nsun6	T	C	2: 15,039,825 (GRCm38)	Q110R	probably benign	Het
Olfr22-ps1	C	T	11: 73,955,184 (GRCm38)	Q165*	probably null	Het
Olfr668	C	T	7: 104,925,056 (GRCm38)	R236H	probably benign	Het
Olfr851	T	A	9: 19,497,522 (GRCm38)	V258E	probably damaging	Het
Pappa2	T	C	1: 158,851,131 (GRCm38)	D905G	probably damaging	Het
Pepd	T	C	7: 34,969,540 (GRCm38)	L195S	probably damaging	Het
Pms1	A	G	1: 53,207,316 (GRCm38)	S355P	probably benign	Het
Pop1	A	T	15: 34,502,413 (GRCm38)	K82M	probably damaging	Het
Ppp1r9b	T	A	11: 95,001,940 (GRCm38)	D655E	possibly damaging	Het
Ppt2	A	G	17: 34,626,803 (GRCm38)		probably null	Het
Rfc3	A	T	5: 151,651,151 (GRCm38)	M1K	probably null	Het
Rffl	T	C	11: 82,810,148 (GRCm38)	D284G	probably damaging	Het
Robo3	T	C	9: 37,429,646 (GRCm38)	D110G	probably damaging	Het
Rtel1	G	A	2: 181,322,315 (GRCm38)	V36M	probably damaging	Het
Slc1a7	T	A	4: 108,010,486 (GRCm38)	I457K	possibly damaging	Het
Smco1	A	G	16: 32,273,723 (GRCm38)	M71V	probably benign	Het
Snrnp200	G	A	2: 127,227,902 (GRCm38)	S989N	probably damaging	Het
Spef2	T	A	15: 9,713,219 (GRCm38)	I356F	probably damaging	Het
Stk24	C	T	14: 121,302,287 (GRCm38)	A166T	probably damaging	Het
Tada2b	T	C	5: 36,476,767 (GRCm38)	I156V	probably benign	Het
Tbce	C	T	13: 14,019,742 (GRCm38)	V111M	probably damaging	Het
Tlnrd1	A	G	7: 83,882,947 (GRCm38)	L92P	probably damaging	Het
Tnr	T	A	1: 159,886,208 (GRCm38)	D735E	probably benign	Het
Tram1	T	C	1: 13,579,547 (GRCm38)	H110R	probably damaging	Het
Ttll3	CAAAGTAA	CAAAGTAAAGTAA	6: 113,399,157 (GRCm38)		probably null	Het
Unc5b	C	T	10: 60,783,120 (GRCm38)	C81Y	probably damaging	Het
Vps13a	G	A	19: 16,703,789 (GRCm38)	T1041M	probably benign	Het

Other mutations in Prss35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01548:Prss35	APN	9	86,755,274 (GRCm38)	missense	probably benign	0.00
IGL02749:Prss35	APN	9	86,756,244 (GRCm38)	missense	probably damaging	1.00
R0346:Prss35	UTSW	9	86,755,351 (GRCm38)	missense	probably benign	0.04
R0403:Prss35	UTSW	9	86,756,037 (GRCm38)	missense	probably damaging	1.00
R1664:Prss35	UTSW	9	86,755,647 (GRCm38)	missense	probably benign	0.29
R2016:Prss35	UTSW	9	86,755,512 (GRCm38)	missense	probably benign	0.37
R2017:Prss35	UTSW	9	86,755,512 (GRCm38)	missense	probably benign	0.37
R2325:Prss35	UTSW	9	86,756,304 (GRCm38)	missense	probably damaging	1.00
R2429:Prss35	UTSW	9	86,755,345 (GRCm38)	missense	probably benign
R2965:Prss35	UTSW	9	86,755,582 (GRCm38)	missense	probably damaging	1.00
R2966:Prss35	UTSW	9	86,755,582 (GRCm38)	missense	probably damaging	1.00
R3961:Prss35	UTSW	9	86,755,749 (GRCm38)	missense	probably benign	0.02
R4792:Prss35	UTSW	9	86,755,669 (GRCm38)	missense	probably damaging	1.00
R4902:Prss35	UTSW	9	86,756,122 (GRCm38)	missense	probably damaging	1.00
R6169:Prss35	UTSW	9	86,755,438 (GRCm38)	missense	probably benign	0.00
R6446:Prss35	UTSW	9	86,755,653 (GRCm38)	missense	probably damaging	0.99
R6753:Prss35	UTSW	9	86,756,100 (GRCm38)	missense	probably damaging	1.00
R7008:Prss35	UTSW	9	86,756,308 (GRCm38)	missense	probably benign	0.01
R7387:Prss35	UTSW	9	86,755,921 (GRCm38)	missense	probably damaging	1.00
R7523:Prss35	UTSW	9	86,755,374 (GRCm38)	missense	probably damaging	1.00
R7652:Prss35	UTSW	9	86,755,970 (GRCm38)	missense	probably benign
R8013:Prss35	UTSW	9	86,755,425 (GRCm38)	missense	probably damaging	1.00
R8194:Prss35	UTSW	9	86,755,613 (GRCm38)	missense	possibly damaging	0.94
R8871:Prss35	UTSW	9	86,755,191 (GRCm38)	missense	possibly damaging	0.84
R9444:Prss35	UTSW	9	86,756,104 (GRCm38)	missense	probably damaging	1.00
R9462:Prss35	UTSW	9	86,756,339 (GRCm38)	missense
R9695:Prss35	UTSW	9	86,755,708 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGAAAATACGTTACTCTGGTTGG -3'
(R):5'- AAGATGCTGAACCTGCTGTC -3'

Sequencing Primer
(F):5'- ACTCTGGTTGGTAATTTTAATCCCTG -3'
(R):5'- ATGCTGAACCTGCTGTCCGTAC -3'

Posted On

2019-10-24