Mutagenetix > Incidental Mutation

Incidental Mutation 'R7588:Cdk15'

587240

Institutional Source

Beutler Lab

Gene Symbol

Cdk15

Ensembl Gene

ENSMUSG00000026023

Gene Name

cyclin dependent kinase 15

Synonyms

Pftk2, Als2cr7

MMRRC Submission

045636-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7588 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

59296029-59391656 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 59383458 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 415 (D415G)

Ref Sequence

ENSEMBL: ENSMUSP00000109886 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000114248] [ENSMUST00000160662]

AlphaFold

Q3V3A1

Predicted Effect

possibly damaging
Transcript: ENSMUST00000114248
AA Change: D415G

PolyPhen 2 Score 0.951 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000109886
Gene: ENSMUSG00000026023
AA Change: D415G

Domain	Start	End	E-Value	Type
S_TKc	101	385	7.9e-86	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000160662
AA Change: D417G

PolyPhen 2 Score 0.951 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000124680
Gene: ENSMUSG00000026023
AA Change: D417G

Domain	Start	End	E-Value	Type
S_TKc	103	387	7.9e-86	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 98.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ap2b1	T	A	11: 83,215,348 (GRCm39)	D97E	probably benign	Het
Apbb1	G	T	7: 105,223,173 (GRCm39)	P146Q	probably benign	Het
Aste1	T	A	9: 105,274,590 (GRCm39)	S277T	possibly damaging	Het
Bnip5	T	C	17: 29,124,430 (GRCm39)	K291E	probably benign	Het
Cep152	T	A	2: 125,411,546 (GRCm39)	E1256D	probably damaging	Het
Chd7	A	G	4: 8,864,039 (GRCm39)	N2643S	probably damaging	Het
Copg2	A	G	6: 30,788,526 (GRCm39)		probably null	Het
D130043K22Rik	T	A	13: 25,071,876 (GRCm39)	I940N	probably damaging	Het
Dnmt3a	A	G	12: 3,946,080 (GRCm39)	T312A	possibly damaging	Het
Dock5	A	G	14: 68,000,607 (GRCm39)		probably null	Het
Fam83g	A	T	11: 61,575,522 (GRCm39)	I55F	probably damaging	Het
Fanci	T	C	7: 79,084,017 (GRCm39)	F780L	possibly damaging	Het
Fhod3	G	T	18: 25,223,305 (GRCm39)	A884S	probably benign	Het
Gas6	T	C	8: 13,516,711 (GRCm39)	S596G	probably benign	Het
Gns	T	C	10: 121,226,563 (GRCm39)	V404A	probably benign	Het
Gpatch1	T	C	7: 34,991,173 (GRCm39)	N624D	probably damaging	Het
Hmcn1	T	C	1: 150,532,885 (GRCm39)	I3099M	possibly damaging	Het
Itga1	A	T	13: 115,104,785 (GRCm39)	S1080R	possibly damaging	Het
Kcnk15	T	C	2: 163,700,226 (GRCm39)	V155A	probably damaging	Het
Luzp2	T	A	7: 54,724,838 (GRCm39)		probably null	Het
Mapk8ip1	T	A	2: 92,216,984 (GRCm39)	D446V	possibly damaging	Het
Med1	T	C	11: 98,046,398 (GRCm39)	E1466G	unknown	Het
Mug1	A	G	6: 121,852,476 (GRCm39)	R855G	probably damaging	Het
Naalad2	C	A	9: 18,262,775 (GRCm39)	V374F	probably damaging	Het
Naip5	T	C	13: 100,356,204 (GRCm39)	Q1137R	probably benign	Het
Naip5	G	T	13: 100,356,205 (GRCm39)	Q1137K	not run	Het
Nlrp5	A	G	7: 23,107,576 (GRCm39)	E83G	probably benign	Het
Nod2	T	C	8: 89,401,536 (GRCm39)	F901S	possibly damaging	Het
Nps	T	C	7: 134,870,508 (GRCm39)	V10A	probably benign	Het
Pbrm1	T	A	14: 30,806,900 (GRCm39)	V1109E	probably damaging	Het
Pcdh7	A	G	5: 57,877,246 (GRCm39)	D267G	probably damaging	Het
Pfkp	A	G	13: 6,698,673 (GRCm39)	Y15H	possibly damaging	Het
Pgap6	T	C	17: 26,341,017 (GRCm39)	Y176H	probably damaging	Het
Ppm1b	T	C	17: 85,320,997 (GRCm39)	S380P	probably benign	Het
Psg21	T	C	7: 18,381,134 (GRCm39)	N470D	probably benign	Het
Qrich2	T	C	11: 116,356,763 (GRCm39)	N29D	possibly damaging	Het
Reln	T	C	5: 22,090,566 (GRCm39)	T3431A	probably benign	Het
Rfc1	A	G	5: 65,429,850 (GRCm39)	V852A	probably damaging	Het
Rttn	T	G	18: 89,082,353 (GRCm39)	D1426E	probably damaging	Het
St3gal1	A	G	15: 66,983,195 (GRCm39)	V187A	possibly damaging	Het
Styxl1	G	A	5: 135,799,130 (GRCm39)	P28L	probably damaging	Het
Tcl1b4	A	G	12: 105,168,641 (GRCm39)		probably benign	Het
Trav17	A	G	14: 54,044,302 (GRCm39)	D24G	probably benign	Het
Trim28	A	C	7: 12,763,347 (GRCm39)	D496A	probably damaging	Het
Trim29	T	C	9: 43,246,425 (GRCm39)	Y574H	probably damaging	Het
Trip12	G	T	1: 84,738,604 (GRCm39)	F750L	probably damaging	Het
Ubr3	C	A	2: 69,801,513 (GRCm39)	T1007K	probably damaging	Het
Zzz3	A	G	3: 152,128,405 (GRCm39)	Y7C	possibly damaging	Het

Other mutations in Cdk15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01307:Cdk15	APN	1	59,326,955 (GRCm39)	missense	probably benign	0.37
IGL01612:Cdk15	APN	1	59,328,932 (GRCm39)	missense	possibly damaging	0.93
IGL02179:Cdk15	APN	1	59,370,100 (GRCm39)	missense	possibly damaging	0.84
IGL03228:Cdk15	APN	1	59,297,912 (GRCm39)	missense	possibly damaging	0.95
FR4449:Cdk15	UTSW	1	59,296,982 (GRCm39)	small insertion	probably benign
R0270:Cdk15	UTSW	1	59,349,965 (GRCm39)	missense	probably damaging	0.99
R1720:Cdk15	UTSW	1	59,328,917 (GRCm39)	missense	probably damaging	1.00
R1958:Cdk15	UTSW	1	59,383,475 (GRCm39)	missense	probably damaging	0.99
R1969:Cdk15	UTSW	1	59,370,110 (GRCm39)	missense	probably damaging	1.00
R3159:Cdk15	UTSW	1	59,340,440 (GRCm39)	missense	probably damaging	1.00
R6000:Cdk15	UTSW	1	59,328,818 (GRCm39)	missense	probably damaging	1.00
R6226:Cdk15	UTSW	1	59,304,792 (GRCm39)	missense	probably damaging	1.00
R6257:Cdk15	UTSW	1	59,296,264 (GRCm39)	critical splice donor site	probably null
R7184:Cdk15	UTSW	1	59,304,814 (GRCm39)	missense	probably benign	0.33
R7446:Cdk15	UTSW	1	59,328,854 (GRCm39)	missense	probably damaging	1.00
R7467:Cdk15	UTSW	1	59,328,938 (GRCm39)	missense	probably null	0.96
R8540:Cdk15	UTSW	1	59,349,992 (GRCm39)	missense	possibly damaging	0.93
R9024:Cdk15	UTSW	1	59,326,957 (GRCm39)	missense	probably damaging	1.00
R9372:Cdk15	UTSW	1	59,370,142 (GRCm39)	missense	probably benign	0.00
R9404:Cdk15	UTSW	1	59,328,914 (GRCm39)	missense	possibly damaging	0.55
R9493:Cdk15	UTSW	1	59,326,943 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGGTGCACTTGACAACTATTAC -3'
(R):5'- GACAAAGGCAGACCCTGTTC -3'

Sequencing Primer
(F):5'- ACCCCAAGTCAGTATAGTCAGTTTGC -3'
(R):5'- AAAGGCAGACCCTGTTCCCTTC -3'

Posted On

2019-10-24