Mutagenetix > Incidental Mutation

Incidental Mutation 'R7591:Vmn2r16'

587401

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r16

Ensembl Gene

ENSMUSG00000092080

Gene Name

vomeronasal 2, receptor 16

Synonyms

EG384220

MMRRC Submission

045638-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.170) question?

Stock #

R7591 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

109478247-109512347 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 109510223 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 535 (D535E)

Ref Sequence

ENSEMBL: ENSMUSP00000127838 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165180]

AlphaFold

A0A3B2WCY4

Predicted Effect

probably damaging
Transcript: ENSMUST00000165180
AA Change: D535E

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000127838
Gene: ENSMUSG00000092080
AA Change: D535E

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	76	425	2.3e-28	PFAM
Pfam:NCD3G	509	563	8.2e-19	PFAM
Pfam:7tm_3	596	831	3.5e-56	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

100% (45/45)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ccne2	T	C	4: 11,201,393 (GRCm39)	I307T	probably benign	Het
Chrm3	A	T	13: 9,927,349 (GRCm39)	C562*	probably null	Het
Cldn11	A	T	3: 31,204,436 (GRCm39)	E46D	probably benign	Het
Cyp4a14	A	G	4: 115,347,157 (GRCm39)		probably null	Het
Dbh	A	G	2: 27,060,522 (GRCm39)	T233A	probably damaging	Het
Dennd2c	T	A	3: 103,040,661 (GRCm39)	Y309N	possibly damaging	Het
Dnttip2	T	A	3: 122,070,117 (GRCm39)	L444*	probably null	Het
Enpp5	G	A	17: 44,396,155 (GRCm39)	G356S	probably damaging	Het
Epop	A	T	11: 97,519,158 (GRCm39)	V317E	probably damaging	Het
Eppk1	T	C	15: 75,991,797 (GRCm39)	T1695A	possibly damaging	Het
Fam149a	T	A	8: 45,803,472 (GRCm39)	I421F	possibly damaging	Het
Impa2	T	C	18: 67,451,480 (GRCm39)	L258P	probably damaging	Het
Itga1	G	A	13: 115,119,315 (GRCm39)	R855W	probably damaging	Het
Kcnh5	T	C	12: 75,054,541 (GRCm39)	T468A	probably benign	Het
Kif11	A	G	19: 37,372,711 (GRCm39)	K33R	probably damaging	Het
Lamb1	C	A	12: 31,376,647 (GRCm39)	A1657E	probably damaging	Het
Macc1	T	C	12: 119,410,393 (GRCm39)	V387A	probably damaging	Het
Nalcn	T	C	14: 123,561,297 (GRCm39)	T734A	probably benign	Het
Nphp3	T	C	9: 103,895,477 (GRCm39)		probably null	Het
Nutm2	A	G	13: 50,627,903 (GRCm39)	I461M	probably damaging	Het
Or14p1	T	C	13: 65,292,462 (GRCm39)	F130L	probably benign	Het
Or4p7	G	A	2: 88,222,220 (GRCm39)	V210M	probably benign	Het
Or4s2	A	C	2: 88,473,811 (GRCm39)	K233N	probably damaging	Het
Or5b112	A	G	19: 13,319,619 (GRCm39)	S166G	probably benign	Het
Pigo	T	C	4: 43,025,093 (GRCm39)	N2S	probably benign	Het
Plcl1	A	T	1: 55,736,608 (GRCm39)	I650L	probably benign	Het
Ppcdc	A	T	9: 57,342,262 (GRCm39)	V20D	probably damaging	Het
Pramel27	A	T	4: 143,577,481 (GRCm39)	I88L	probably benign	Het
Rhobtb2	A	G	14: 70,037,190 (GRCm39)	V78A	possibly damaging	Het
Rp1l1	T	C	14: 64,263,558 (GRCm39)	V226A	probably damaging	Het
Scrt1	C	A	15: 76,403,694 (GRCm39)	G99C	probably damaging	Het
Skint9	A	T	4: 112,248,147 (GRCm39)	I199N	probably damaging	Het
Slc5a4a	T	C	10: 75,983,501 (GRCm39)		probably benign	Het
Smad3	C	T	9: 63,561,999 (GRCm39)	W326*	probably null	Het
Spata18	A	T	5: 73,829,759 (GRCm39)	I305F		Het
Spata31h1	C	A	10: 82,128,046 (GRCm39)	V1655F	probably benign	Het
St3gal1	A	G	15: 66,983,195 (GRCm39)	V187A	possibly damaging	Het
Syne3	A	G	12: 104,906,863 (GRCm39)		probably null	Het
Trim69	G	A	2: 121,998,454 (GRCm39)	R142Q	probably benign	Het
Vmn1r40	A	T	6: 89,691,755 (GRCm39)	I191F	probably benign	Het
Vwa5b2	A	G	16: 20,420,317 (GRCm39)	E742G	probably damaging	Het
Zfp442	A	T	2: 150,250,092 (GRCm39)	Y603*	probably null	Het
Zp1	T	C	19: 10,896,835 (GRCm39)	N68S	probably damaging	Het

Other mutations in Vmn2r16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01116:Vmn2r16	APN	5	109,488,294 (GRCm39)	missense	probably damaging	1.00
IGL01374:Vmn2r16	APN	5	109,478,283 (GRCm39)	missense	probably benign	0.00
IGL01391:Vmn2r16	APN	5	109,511,627 (GRCm39)	missense	possibly damaging	0.50
IGL01419:Vmn2r16	APN	5	109,510,267 (GRCm39)	splice site	probably benign
IGL01634:Vmn2r16	APN	5	109,488,177 (GRCm39)	missense	probably benign	0.00
IGL01845:Vmn2r16	APN	5	109,511,762 (GRCm39)	missense	probably damaging	1.00
IGL01875:Vmn2r16	APN	5	109,478,277 (GRCm39)	missense	probably benign	0.01
IGL01910:Vmn2r16	APN	5	109,487,951 (GRCm39)	missense	probably damaging	1.00
IGL02217:Vmn2r16	APN	5	109,487,676 (GRCm39)	missense	probably damaging	0.98
IGL02327:Vmn2r16	APN	5	109,487,987 (GRCm39)	missense	probably benign	0.01
IGL02491:Vmn2r16	APN	5	109,487,703 (GRCm39)	nonsense	probably null
IGL02531:Vmn2r16	APN	5	109,488,134 (GRCm39)	missense	probably damaging	0.99
IGL02680:Vmn2r16	APN	5	109,487,948 (GRCm39)	missense	probably benign	0.44
IGL02884:Vmn2r16	APN	5	109,508,757 (GRCm39)	missense	possibly damaging	0.94
IGL03084:Vmn2r16	APN	5	109,478,292 (GRCm39)	missense	probably damaging	0.99
IGL03096:Vmn2r16	APN	5	109,487,751 (GRCm39)	missense	probably damaging	0.99
IGL03355:Vmn2r16	APN	5	109,511,580 (GRCm39)	missense	possibly damaging	0.74
R0280:Vmn2r16	UTSW	5	109,488,005 (GRCm39)	missense	possibly damaging	0.88
R0594:Vmn2r16	UTSW	5	109,511,762 (GRCm39)	missense	probably damaging	1.00
R1016:Vmn2r16	UTSW	5	109,487,754 (GRCm39)	missense	probably damaging	1.00
R1109:Vmn2r16	UTSW	5	109,487,652 (GRCm39)	missense	probably damaging	0.98
R1248:Vmn2r16	UTSW	5	109,508,643 (GRCm39)	missense	probably benign	0.10
R1626:Vmn2r16	UTSW	5	109,511,443 (GRCm39)	missense	probably damaging	1.00
R1909:Vmn2r16	UTSW	5	109,511,853 (GRCm39)	missense	probably benign	0.01
R1929:Vmn2r16	UTSW	5	109,487,124 (GRCm39)	missense	possibly damaging	0.92
R1982:Vmn2r16	UTSW	5	109,511,890 (GRCm39)	missense	probably benign	0.01
R3038:Vmn2r16	UTSW	5	109,487,199 (GRCm39)	missense	probably damaging	1.00
R3437:Vmn2r16	UTSW	5	109,478,362 (GRCm39)	missense	probably damaging	0.99
R3734:Vmn2r16	UTSW	5	109,478,280 (GRCm39)	missense	probably benign	0.11
R3820:Vmn2r16	UTSW	5	109,510,143 (GRCm39)	missense	probably benign	0.36
R3873:Vmn2r16	UTSW	5	109,488,177 (GRCm39)	missense	probably benign	0.33
R4165:Vmn2r16	UTSW	5	109,478,427 (GRCm39)	missense	possibly damaging	0.80
R4373:Vmn2r16	UTSW	5	109,511,667 (GRCm39)	missense	probably damaging	1.00
R4575:Vmn2r16	UTSW	5	109,511,665 (GRCm39)	missense	possibly damaging	0.81
R4576:Vmn2r16	UTSW	5	109,511,665 (GRCm39)	missense	possibly damaging	0.81
R4578:Vmn2r16	UTSW	5	109,511,665 (GRCm39)	missense	possibly damaging	0.81
R4637:Vmn2r16	UTSW	5	109,478,280 (GRCm39)	missense	probably benign	0.00
R4696:Vmn2r16	UTSW	5	109,487,168 (GRCm39)	missense	probably benign	0.01
R5026:Vmn2r16	UTSW	5	109,508,722 (GRCm39)	nonsense	probably null
R5180:Vmn2r16	UTSW	5	109,478,391 (GRCm39)	missense	probably benign	0.01
R5433:Vmn2r16	UTSW	5	109,511,708 (GRCm39)	missense	probably damaging	1.00
R5955:Vmn2r16	UTSW	5	109,511,613 (GRCm39)	missense	possibly damaging	0.78
R5958:Vmn2r16	UTSW	5	109,510,153 (GRCm39)	missense	possibly damaging	0.81
R6353:Vmn2r16	UTSW	5	109,488,119 (GRCm39)	missense	probably benign	0.33
R6389:Vmn2r16	UTSW	5	109,478,344 (GRCm39)	missense	probably benign	0.19
R6819:Vmn2r16	UTSW	5	109,488,412 (GRCm39)	missense	probably benign	0.04
R6994:Vmn2r16	UTSW	5	109,487,969 (GRCm39)	missense	probably damaging	1.00
R7061:Vmn2r16	UTSW	5	109,511,620 (GRCm39)	missense	probably damaging	0.99
R7063:Vmn2r16	UTSW	5	109,511,650 (GRCm39)	missense	probably damaging	1.00
R7220:Vmn2r16	UTSW	5	109,508,772 (GRCm39)	missense	probably damaging	0.97
R7268:Vmn2r16	UTSW	5	109,488,331 (GRCm39)	nonsense	probably null
R7420:Vmn2r16	UTSW	5	109,511,736 (GRCm39)	missense	probably damaging	0.96
R7644:Vmn2r16	UTSW	5	109,487,837 (GRCm39)	missense	probably damaging	1.00
R7939:Vmn2r16	UTSW	5	109,487,705 (GRCm39)	missense	possibly damaging	0.79
R7977:Vmn2r16	UTSW	5	109,488,015 (GRCm39)	missense	probably damaging	1.00
R7987:Vmn2r16	UTSW	5	109,488,015 (GRCm39)	missense	probably damaging	1.00
R8023:Vmn2r16	UTSW	5	109,488,272 (GRCm39)	nonsense	probably null
R8427:Vmn2r16	UTSW	5	109,488,138 (GRCm39)	missense	probably benign	0.03
R8436:Vmn2r16	UTSW	5	109,511,649 (GRCm39)	missense	probably damaging	1.00
R8475:Vmn2r16	UTSW	5	109,487,073 (GRCm39)	missense	probably benign	0.01
R8554:Vmn2r16	UTSW	5	109,511,997 (GRCm39)	missense	probably benign	0.13
R8771:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8774:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8774-TAIL:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8775:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8775-TAIL:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8777:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8777-TAIL:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8825:Vmn2r16	UTSW	5	109,487,019 (GRCm39)	missense	probably benign	0.41
R8865:Vmn2r16	UTSW	5	109,487,910 (GRCm39)	missense	probably benign	0.33
R8900:Vmn2r16	UTSW	5	109,511,619 (GRCm39)	missense	probably benign	0.04
R9365:Vmn2r16	UTSW	5	109,488,064 (GRCm39)	missense	probably damaging	0.99
R9683:Vmn2r16	UTSW	5	109,511,677 (GRCm39)	missense	probably damaging	1.00
X0027:Vmn2r16	UTSW	5	109,487,175 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r16	UTSW	5	109,511,779 (GRCm39)	frame shift	probably null
Z1088:Vmn2r16	UTSW	5	109,488,381 (GRCm39)	missense	probably benign	0.03
Z1177:Vmn2r16	UTSW	5	109,487,864 (GRCm39)	missense	possibly damaging	0.79

Predicted Primers

PCR Primer
(F):5'- CTGTTAGCAGAGGAATATGTTTAGCC -3'
(R):5'- TGGCAGGGTTGTAAAATTCTGATAC -3'

Sequencing Primer
(F):5'- GCAGAGGAATATGTTTAGCCATATC -3'
(R):5'- CTGAAACACAGAGATTTTAGG -3'

Posted On

2019-10-24