Mutagenetix > Incidental Mutations

Incidental Mutation 'R0622:Olfr314'

58741

Institutional Source

Beutler Lab

Gene Symbol

Olfr314

Ensembl Gene

ENSMUSG00000059504

Gene Name

olfactory receptor 314

Synonyms

MOR278-2, GA_x6K02T2NKPP-622179-621244

MMRRC Submission

038811-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0622 (G1)

Quality Score

207

Status

Not validated

Chromosome

Chromosomal Location

58782952-58788768 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 58786341 bp

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 36 (S36P)

Ref Sequence

ENSEMBL: ENSMUSP00000145387 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076393] [ENSMUST00000205189]

Predicted Effect

probably damaging
Transcript: ENSMUST00000076393
AA Change: S36P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000075729
Gene: ENSMUSG00000059504
AA Change: S36P

Domain	Start	End	E-Value	Type
Pfam:7tm_4	26	306	2.8e-50	PFAM
Pfam:7TM_GPCR_Srsx	33	249	2.1e-6	PFAM
Pfam:7tm_1	39	288	2.3e-26	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000205189
AA Change: S36P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000145387
Gene: ENSMUSG00000059504
AA Change: S36P

Domain	Start	End	E-Value	Type
Pfam:7tm_4	26	259	8.9e-42	PFAM
Pfam:7TM_GPCR_Srsx	33	246	9.4e-6	PFAM
Pfam:7tm_1	39	259	1.1e-25	PFAM

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.7%
20x: 95.9%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agtr1a	T	A	13: 30,381,681	M243K	probably benign	Het
Ap1b1	A	G	11: 5,037,707	M744V	probably damaging	Het
C87977	T	C	4: 144,213,013		probably benign	Het
Ccdc152	T	C	15: 3,298,178	N39S	probably damaging	Het
Cd163	G	A	6: 124,317,352	V490M	probably damaging	Het
Col6a5	G	T	9: 105,925,852	H1305N	unknown	Het
Cpb1	C	A	3: 20,249,818	D361Y	probably damaging	Het
Dchs1	T	A	7: 105,763,449	Y1248F	probably damaging	Het
Dhdds	G	C	4: 133,994,236	F83L	probably damaging	Het
Dsg4	T	A	18: 20,449,788	V161E	possibly damaging	Het
Exosc4	A	G	15: 76,327,536	D15G	probably damaging	Het
F3	A	T	3: 121,725,019	D44V	probably damaging	Het
Fat2	A	T	11: 55,283,128	F2253Y	probably damaging	Het
Fbn1	T	C	2: 125,379,024	D650G	possibly damaging	Het
Gramd4	T	A	15: 86,091,389	F36I	probably damaging	Het
Grm7	G	A	6: 111,358,496	A623T	probably damaging	Het
Gys1	A	T	7: 45,439,995	T193S	probably damaging	Het
Hectd4	A	G	5: 121,348,625	T3228A	possibly damaging	Het
Itpk1	G	T	12: 102,573,980	D281E	probably damaging	Het
Kcnh7	A	C	2: 62,837,289		probably null	Het
Klhl29	A	G	12: 5,081,224	L852P	probably damaging	Het
Lrch1	T	C	14: 74,796,051	Y509C	probably benign	Het
Lrp1b	A	G	2: 41,728,551		probably null	Het
Mcpt4	C	A	14: 56,060,662	R144L	probably benign	Het
Mia2	C	T	12: 59,131,578	R12W	probably damaging	Het
Mrps5	A	G	2: 127,594,531	K116R	probably benign	Het
Myrf	G	A	19: 10,223,452	P286S	probably damaging	Het
Nanp	A	G	2: 151,039,244	M28T	probably benign	Het
Neb	T	C	2: 52,212,951	I4472V	probably benign	Het
Nfix	A	C	8: 84,726,482	N314K	probably damaging	Het
Nlrc3	C	T	16: 3,953,968	R849Q	probably benign	Het
Nup210l	G	A	3: 90,167,740	V786M	probably damaging	Het
Olfr1346	T	C	7: 6,474,599	I163T	possibly damaging	Het
Olfr600	A	G	7: 103,346,857	S24P	probably damaging	Het
Olfr898	A	G	9: 38,349,371	N96S	possibly damaging	Het
Pdia4	A	T	6: 47,806,518	F197Y	probably damaging	Het
Phldb1	T	C	9: 44,715,852	D432G	probably damaging	Het
Pik3ca	A	G	3: 32,436,552	E116G	probably damaging	Het
Polq	T	C	16: 37,060,993	V1173A	probably benign	Het
Pou2f3	C	T	9: 43,125,119	R423H	probably damaging	Het
Prkag2	T	C	5: 24,869,249	N246S	probably damaging	Het
Proser1	A	G	3: 53,477,860	S388G	probably benign	Het
Ralgps1	G	A	2: 33,174,447	R238*	probably null	Het
Rfx2	T	C	17: 56,777,071	D657G	probably damaging	Het
Ryr3	A	G	2: 112,662,555	F3724S	probably damaging	Het
Sh2d5	T	C	4: 138,259,228	S421P	probably damaging	Het
Slc17a2	C	A	13: 23,812,611	T33K	probably damaging	Het
St8sia5	A	G	18: 77,246,113	T156A	probably damaging	Het
Stk32c	T	C	7: 139,188,110	D85G	probably benign	Het
Tnks	A	G	8: 34,940,822	S251P	probably damaging	Het
Tnxb	T	A	17: 34,718,729	L3864Q	probably damaging	Het
Trim9	A	G	12: 70,346,604	Y189H	probably damaging	Het
Vmn1r77	T	G	7: 12,041,388	F30L	probably benign	Het
Wasf3	A	G	5: 146,466,792		probably null	Het
Wdr90	C	T	17: 25,855,658	C603Y	probably damaging	Het
Zdhhc25	T	C	15: 88,601,107	L215P	probably damaging	Het
Zeb1	C	T	18: 5,759,123	Q140*	probably null	Het
Zfp677	C	T	17: 21,397,700	L340F	probably benign	Het

Other mutations in Olfr314

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02036:Olfr314	APN	11	58787097	missense	probably damaging	1.00
IGL02268:Olfr314	APN	11	58786725	missense	probably benign	0.39
IGL02743:Olfr314	APN	11	58786780	nonsense	probably null
R0157:Olfr314	UTSW	11	58787059	missense	probably damaging	1.00
R0926:Olfr314	UTSW	11	58787109	missense	probably damaging	1.00
R1985:Olfr314	UTSW	11	58786384	missense	probably damaging	0.99
R2273:Olfr314	UTSW	11	58786666	missense	probably benign	0.00
R2274:Olfr314	UTSW	11	58786666	missense	probably benign	0.00
R5642:Olfr314	UTSW	11	58786828	missense	probably damaging	1.00
R5770:Olfr314	UTSW	11	58786594	missense	probably benign	0.39
R6292:Olfr314	UTSW	11	58786237	start codon destroyed	probably null	0.94
R6813:Olfr314	UTSW	11	58786646	missense	probably benign	0.06
R7285:Olfr314	UTSW	11	58786484	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TGCGTACTTCACACAGCATCACAG -3'
(R):5'- TGCTAAGAGGAAGCACTCTCCACC -3'

Sequencing Primer
(F):5'- AGGTGAGGAAACTCCTCTGTC -3'
(R):5'- GATCTGGAAGCCACAGCC -3'

Posted On

2013-07-11