Mutagenetix > Incidental Mutation

Incidental Mutation 'R7600:Zcwpw1'

587959

Institutional Source

Beutler Lab

Gene Symbol

Zcwpw1

Ensembl Gene

ENSMUSG00000037108

Gene Name

zinc finger, CW type with PWWP domain 1

Synonyms

LOC381678

MMRRC Submission

045642-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.139) question?

Stock #

R7600 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

137786060-137820883 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 137798396 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Stop codon at position 198 (K198*)

Ref Sequence

ENSEMBL: ENSMUSP00000048730 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035852]

AlphaFold

Q6IR42

Predicted Effect

probably null
Transcript: ENSMUST00000035852
AA Change: K198*

SMART Domains

Protein: ENSMUSP00000048730
Gene: ENSMUSG00000037108
AA Change: K198*

Domain	Start	End	E-Value	Type
Pfam:zf-CW	246	293	7.3e-18	PFAM
Pfam:PWWP	306	401	6.9e-22	PFAM
coiled coil region	440	462	N/A	INTRINSIC
low complexity region	587	598	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (45/45)

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam25	G	A	8: 41,208,854 (GRCm39)	V707I	probably benign	Het
Ahnak	A	T	19: 8,981,938 (GRCm39)	D1074V	possibly damaging	Het
Aldh4a1	A	G	4: 139,372,315 (GRCm39)	M495V	probably benign	Het
Atxn1	T	G	13: 45,710,536 (GRCm39)	K799Q	possibly damaging	Het
Catsperg2	A	T	7: 29,404,283 (GRCm39)	S831T	probably benign	Het
Cldn1	T	C	16: 26,179,669 (GRCm39)	T133A	probably benign	Het
Clec12b	A	G	6: 129,353,226 (GRCm39)	C254R	probably damaging	Het
Cnnm2	A	T	19: 46,750,506 (GRCm39)	T99S	probably benign	Het
Col6a4	T	G	9: 105,944,198 (GRCm39)	D1092A	possibly damaging	Het
Dnajc9	A	G	14: 20,438,793 (GRCm39)	L20P	probably damaging	Het
Dsp	C	A	13: 38,375,691 (GRCm39)	Q1159K	probably damaging	Het
Dst	C	T	1: 34,306,011 (GRCm39)	T4110I	probably damaging	Het
Epb42	A	G	2: 120,852,307 (GRCm39)	L562P	probably damaging	Het
Fras1	T	C	5: 96,832,295 (GRCm39)	Y1543H	probably damaging	Het
Igkv1-117	T	A	6: 68,098,100 (GRCm39)	V9E	possibly damaging	Het
Kbtbd8	T	A	6: 95,099,573 (GRCm39)	Y361N	probably damaging	Het
Lrp1	C	T	10: 127,391,575 (GRCm39)	R2948H	probably benign	Het
Lrp10	G	T	14: 54,706,852 (GRCm39)	R563L	possibly damaging	Het
Mllt3	G	T	4: 87,759,456 (GRCm39)	H197Q	probably benign	Het
Myo18b	G	A	5: 113,025,969 (GRCm39)	P27L	unknown	Het
Nlrp1a	C	A	11: 70,989,740 (GRCm39)	R1110L	probably damaging	Het
Nol10	T	G	12: 17,419,481 (GRCm39)	D257E	probably damaging	Het
Olfm3	T	C	3: 114,890,589 (GRCm39)	V114A	possibly damaging	Het
Or2a57	C	A	6: 43,212,770 (GRCm39)	T76K	probably damaging	Het
Or2t43	T	C	11: 58,458,162 (GRCm39)	N3S	probably benign	Het
Pdzd2	G	T	15: 12,372,820 (GRCm39)	D2438E	probably damaging	Het
Prl7a2	T	G	13: 27,843,264 (GRCm39)	I180L	possibly damaging	Het
Ptch2	A	T	4: 116,953,422 (GRCm39)		probably benign	Het
Snrpn	A	G	7: 59,638,351 (GRCm39)	M1T	probably null	Het
Sntb1	A	T	15: 55,655,584 (GRCm39)	W211R	possibly damaging	Het
Sp110	G	A	1: 85,506,813 (GRCm39)	R417C	probably benign	Het
Sytl2	T	C	7: 90,025,352 (GRCm39)	S447P	probably benign	Het
Tas2r129	G	T	6: 132,928,137 (GRCm39)	G25*	probably null	Het
Tcstv7a	A	T	13: 120,290,232 (GRCm39)		probably null	Het
Tle2	T	G	10: 81,422,147 (GRCm39)	Y396*	probably null	Het
Trpm2	T	C	10: 77,773,885 (GRCm39)	K510R	probably benign	Het
Trpm3	A	G	19: 22,903,458 (GRCm39)	T1073A	possibly damaging	Het
Ttc24	A	T	3: 87,979,320 (GRCm39)	M1K	probably null	Het
Tubgcp5	A	G	7: 55,458,261 (GRCm39)	T391A	probably benign	Het
Vmn1r17	T	C	6: 57,337,906 (GRCm39)	E153G	probably benign	Het
Vmn1r232	A	T	17: 21,133,999 (GRCm39)	N200K	possibly damaging	Het
Zfp142	A	G	1: 74,612,827 (GRCm39)	V641A	probably damaging	Het
Zfp318	C	T	17: 46,695,210 (GRCm39)	A149V	possibly damaging	Het
Zfp608	T	C	18: 55,121,092 (GRCm39)	N165S	probably damaging	Het

Other mutations in Zcwpw1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01296:Zcwpw1	APN	5	137,795,061 (GRCm39)	missense	probably benign	0.42
IGL02316:Zcwpw1	APN	5	137,808,272 (GRCm39)	unclassified	probably benign
IGL02336:Zcwpw1	APN	5	137,808,376 (GRCm39)	missense	probably damaging	0.96
R0103:Zcwpw1	UTSW	5	137,808,375 (GRCm39)	nonsense	probably null
R0103:Zcwpw1	UTSW	5	137,808,375 (GRCm39)	nonsense	probably null
R0295:Zcwpw1	UTSW	5	137,815,734 (GRCm39)	missense	probably damaging	1.00
R0514:Zcwpw1	UTSW	5	137,794,945 (GRCm39)	missense	probably benign	0.26
R0685:Zcwpw1	UTSW	5	137,797,854 (GRCm39)	missense	probably benign	0.00
R0698:Zcwpw1	UTSW	5	137,815,783 (GRCm39)	missense	probably benign
R0727:Zcwpw1	UTSW	5	137,809,069 (GRCm39)	unclassified	probably benign
R1677:Zcwpw1	UTSW	5	137,795,022 (GRCm39)	missense	probably damaging	0.99
R1780:Zcwpw1	UTSW	5	137,794,914 (GRCm39)	missense	probably damaging	0.98
R1938:Zcwpw1	UTSW	5	137,809,884 (GRCm39)	missense	probably damaging	0.99
R2875:Zcwpw1	UTSW	5	137,808,304 (GRCm39)	missense	probably damaging	1.00
R4177:Zcwpw1	UTSW	5	137,798,395 (GRCm39)	missense	probably damaging	0.99
R5073:Zcwpw1	UTSW	5	137,793,781 (GRCm39)	start codon destroyed	probably null	0.81
R5913:Zcwpw1	UTSW	5	137,798,269 (GRCm39)	missense	probably benign	0.31
R6224:Zcwpw1	UTSW	5	137,810,298 (GRCm39)	missense	possibly damaging	0.63
R6542:Zcwpw1	UTSW	5	137,810,282 (GRCm39)	missense	probably damaging	0.98
R7204:Zcwpw1	UTSW	5	137,810,346 (GRCm39)	missense	probably damaging	0.98
R7542:Zcwpw1	UTSW	5	137,817,785 (GRCm39)	missense	probably benign	0.00
R7911:Zcwpw1	UTSW	5	137,795,032 (GRCm39)	missense	probably null	1.00
R7972:Zcwpw1	UTSW	5	137,799,323 (GRCm39)	missense	probably benign	0.13
R7988:Zcwpw1	UTSW	5	137,815,753 (GRCm39)	missense	possibly damaging	0.69
R8174:Zcwpw1	UTSW	5	137,817,839 (GRCm39)	critical splice donor site	probably null
R8713:Zcwpw1	UTSW	5	137,797,794 (GRCm39)	missense	probably benign	0.06
R8851:Zcwpw1	UTSW	5	137,820,626 (GRCm39)	missense	probably damaging	1.00
R9016:Zcwpw1	UTSW	5	137,798,340 (GRCm39)	missense	probably damaging	0.98
R9131:Zcwpw1	UTSW	5	137,809,182 (GRCm39)	missense	probably damaging	1.00
R9337:Zcwpw1	UTSW	5	137,799,274 (GRCm39)	missense	probably benign	0.37
X0021:Zcwpw1	UTSW	5	137,809,869 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATGGCAAGTGTCTCTCTGAAG -3'
(R):5'- GGTTTAAAGGATATCTGGGATCTAGAG -3'

Sequencing Primer
(F):5'- GGAAGTGAATTCTGAGATGG -3'
(R):5'- CTTTGGGAACTGCAACCCTATAGG -3'

Posted On

2019-10-24