Mutagenetix > Incidental Mutation

Incidental Mutation 'R7600:Prl7a2'

587978

Institutional Source

Beutler Lab

Gene Symbol

Prl7a2

Ensembl Gene

ENSMUSG00000046899

Gene Name

prolactin family 7, subfamily a, member 2

Synonyms

Prlpf, PLP-F

MMRRC Submission

045642-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.057) question?

Stock #

R7600 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

27842567-27852019 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 27843264 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 180 (I180L)

Ref Sequence

ENSEMBL: ENSMUSP00000006660 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000006660]

AlphaFold

O54831

Predicted Effect

possibly damaging
Transcript: ENSMUST00000006660
AA Change: I180L

PolyPhen 2 Score 0.630 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000006660
Gene: ENSMUSG00000046899
AA Change: I180L

Domain	Start	End	E-Value	Type
Pfam:Hormone_1	17	244	6.9e-45	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (45/45)

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam25	G	A	8: 41,208,854 (GRCm39)	V707I	probably benign	Het
Ahnak	A	T	19: 8,981,938 (GRCm39)	D1074V	possibly damaging	Het
Aldh4a1	A	G	4: 139,372,315 (GRCm39)	M495V	probably benign	Het
Atxn1	T	G	13: 45,710,536 (GRCm39)	K799Q	possibly damaging	Het
Catsperg2	A	T	7: 29,404,283 (GRCm39)	S831T	probably benign	Het
Cldn1	T	C	16: 26,179,669 (GRCm39)	T133A	probably benign	Het
Clec12b	A	G	6: 129,353,226 (GRCm39)	C254R	probably damaging	Het
Cnnm2	A	T	19: 46,750,506 (GRCm39)	T99S	probably benign	Het
Col6a4	T	G	9: 105,944,198 (GRCm39)	D1092A	possibly damaging	Het
Dnajc9	A	G	14: 20,438,793 (GRCm39)	L20P	probably damaging	Het
Dsp	C	A	13: 38,375,691 (GRCm39)	Q1159K	probably damaging	Het
Dst	C	T	1: 34,306,011 (GRCm39)	T4110I	probably damaging	Het
Epb42	A	G	2: 120,852,307 (GRCm39)	L562P	probably damaging	Het
Fras1	T	C	5: 96,832,295 (GRCm39)	Y1543H	probably damaging	Het
Igkv1-117	T	A	6: 68,098,100 (GRCm39)	V9E	possibly damaging	Het
Kbtbd8	T	A	6: 95,099,573 (GRCm39)	Y361N	probably damaging	Het
Lrp1	C	T	10: 127,391,575 (GRCm39)	R2948H	probably benign	Het
Lrp10	G	T	14: 54,706,852 (GRCm39)	R563L	possibly damaging	Het
Mllt3	G	T	4: 87,759,456 (GRCm39)	H197Q	probably benign	Het
Myo18b	G	A	5: 113,025,969 (GRCm39)	P27L	unknown	Het
Nlrp1a	C	A	11: 70,989,740 (GRCm39)	R1110L	probably damaging	Het
Nol10	T	G	12: 17,419,481 (GRCm39)	D257E	probably damaging	Het
Olfm3	T	C	3: 114,890,589 (GRCm39)	V114A	possibly damaging	Het
Or2a57	C	A	6: 43,212,770 (GRCm39)	T76K	probably damaging	Het
Or2t43	T	C	11: 58,458,162 (GRCm39)	N3S	probably benign	Het
Pdzd2	G	T	15: 12,372,820 (GRCm39)	D2438E	probably damaging	Het
Ptch2	A	T	4: 116,953,422 (GRCm39)		probably benign	Het
Snrpn	A	G	7: 59,638,351 (GRCm39)	M1T	probably null	Het
Sntb1	A	T	15: 55,655,584 (GRCm39)	W211R	possibly damaging	Het
Sp110	G	A	1: 85,506,813 (GRCm39)	R417C	probably benign	Het
Sytl2	T	C	7: 90,025,352 (GRCm39)	S447P	probably benign	Het
Tas2r129	G	T	6: 132,928,137 (GRCm39)	G25*	probably null	Het
Tcstv7a	A	T	13: 120,290,232 (GRCm39)		probably null	Het
Tle2	T	G	10: 81,422,147 (GRCm39)	Y396*	probably null	Het
Trpm2	T	C	10: 77,773,885 (GRCm39)	K510R	probably benign	Het
Trpm3	A	G	19: 22,903,458 (GRCm39)	T1073A	possibly damaging	Het
Ttc24	A	T	3: 87,979,320 (GRCm39)	M1K	probably null	Het
Tubgcp5	A	G	7: 55,458,261 (GRCm39)	T391A	probably benign	Het
Vmn1r17	T	C	6: 57,337,906 (GRCm39)	E153G	probably benign	Het
Vmn1r232	A	T	17: 21,133,999 (GRCm39)	N200K	possibly damaging	Het
Zcwpw1	A	T	5: 137,798,396 (GRCm39)	K198*	probably null	Het
Zfp142	A	G	1: 74,612,827 (GRCm39)	V641A	probably damaging	Het
Zfp318	C	T	17: 46,695,210 (GRCm39)	A149V	possibly damaging	Het
Zfp608	T	C	18: 55,121,092 (GRCm39)	N165S	probably damaging	Het

Other mutations in Prl7a2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01484:Prl7a2	APN	13	27,843,191 (GRCm39)	missense	probably damaging	0.98
IGL02424:Prl7a2	APN	13	27,851,953 (GRCm39)	missense	probably null	0.08
IGL02734:Prl7a2	APN	13	27,843,190 (GRCm39)	missense	probably benign	0.38
IGL02823:Prl7a2	APN	13	27,846,734 (GRCm39)	missense	possibly damaging	0.95
PIT4260001:Prl7a2	UTSW	13	27,843,259 (GRCm39)	nonsense	probably null
R0733:Prl7a2	UTSW	13	27,846,671 (GRCm39)	missense	probably damaging	1.00
R1371:Prl7a2	UTSW	13	27,846,750 (GRCm39)	missense	probably benign	0.01
R1778:Prl7a2	UTSW	13	27,843,254 (GRCm39)	missense	probably damaging	0.98
R1857:Prl7a2	UTSW	13	27,843,163 (GRCm39)	nonsense	probably null
R2063:Prl7a2	UTSW	13	27,844,870 (GRCm39)	missense	probably damaging	0.98
R2064:Prl7a2	UTSW	13	27,844,870 (GRCm39)	missense	probably damaging	0.98
R2065:Prl7a2	UTSW	13	27,844,870 (GRCm39)	missense	probably damaging	0.98
R2067:Prl7a2	UTSW	13	27,844,870 (GRCm39)	missense	probably damaging	0.98
R2068:Prl7a2	UTSW	13	27,844,870 (GRCm39)	missense	probably damaging	0.98
R2176:Prl7a2	UTSW	13	27,843,089 (GRCm39)	missense	probably benign	0.10
R2213:Prl7a2	UTSW	13	27,849,051 (GRCm39)	missense	probably benign	0.06
R4111:Prl7a2	UTSW	13	27,849,050 (GRCm39)	missense	possibly damaging	0.96
R4459:Prl7a2	UTSW	13	27,849,979 (GRCm39)	missense	probably benign	0.21
R4483:Prl7a2	UTSW	13	27,844,930 (GRCm39)	missense	possibly damaging	0.80
R4722:Prl7a2	UTSW	13	27,844,858 (GRCm39)	missense	probably damaging	1.00
R5360:Prl7a2	UTSW	13	27,843,143 (GRCm39)	missense	probably benign	0.22
R5778:Prl7a2	UTSW	13	27,844,983 (GRCm39)	nonsense	probably null
R6667:Prl7a2	UTSW	13	27,845,024 (GRCm39)	missense	probably benign	0.03
R7107:Prl7a2	UTSW	13	27,843,076 (GRCm39)	missense	possibly damaging	0.89
R8298:Prl7a2	UTSW	13	27,844,994 (GRCm39)	missense	probably benign	0.00
R8447:Prl7a2	UTSW	13	27,849,941 (GRCm39)	missense	possibly damaging	0.72
R9009:Prl7a2	UTSW	13	27,849,994 (GRCm39)	missense	probably damaging	1.00
R9331:Prl7a2	UTSW	13	27,849,062 (GRCm39)	missense	probably damaging	1.00
R9624:Prl7a2	UTSW	13	27,849,869 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ATACCACACACTCTAATAGCTTGAG -3'
(R):5'- CCATGGAATGATGCTGAAATCC -3'

Sequencing Primer
(F):5'- AGCTTGAGATAAAGTTCAACCATATG -3'
(R):5'- TCAAAGAGTGAATAGTTACTCCCAC -3'

Posted On

2019-10-24