Mutagenetix > Incidental Mutations

Incidental Mutation 'R7605:Itgb4'

588257

Institutional Source

Beutler Lab

Gene Symbol

Itgb4

Ensembl Gene

ENSMUSG00000020758

Gene Name

integrin beta 4

Synonyms

CD104

MMRRC Submission

Accession Numbers

Is this an essential gene?

Essential (E-score: 1.000) question?

Stock #

R7605 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

115974709-116008412 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 116006476 bp

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 1521 (V1521E)

Ref Sequence

ENSEMBL: ENSMUSP00000102066 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021107] [ENSMUST00000021114] [ENSMUST00000068981] [ENSMUST00000106458] [ENSMUST00000106460] [ENSMUST00000106461] [ENSMUST00000169928]

Predicted Effect

probably benign
Transcript: ENSMUST00000021107
AA Change: V1505E

PolyPhen 2 Score 0.047 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000021107
Gene: ENSMUSG00000020758
AA Change: V1505E

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	712	1.32e-28	SMART
low complexity region	715	732	N/A	INTRINSIC
transmembrane domain	737	756	N/A	INTRINSIC
Calx_beta	980	1085	3.13e-35	SMART
FN3	1125	1203	3.15e-8	SMART
FN3	1218	1305	6.29e-8	SMART
low complexity region	1324	1332	N/A	INTRINSIC
FN3	1508	1589	1.79e-12	SMART
FN3	1621	1705	1.7e-13	SMART
low complexity region	1738	1751	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000021114

SMART Domains

Protein: ENSMUSP00000021114
Gene: ENSMUSG00000020766

Domain	Start	End	E-Value	Type
Pfam:GalKase_gal_bdg	18	67	2.6e-24	PFAM
Pfam:GHMP_kinases_N	126	194	7.3e-14	PFAM
Pfam:GHMP_kinases_C	289	374	1.6e-14	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000068981
AA Change: V1456E

PolyPhen 2 Score 0.127 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000070811
Gene: ENSMUSG00000020758
AA Change: V1456E

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
FN3	1459	1540	1.79e-12	SMART
FN3	1572	1656	1.7e-13	SMART
low complexity region	1689	1702	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106458
AA Change: V1521E

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000102066
Gene: ENSMUSG00000020758
AA Change: V1521E

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
low complexity region	1413	1425	N/A	INTRINSIC
FN3	1524	1605	1.79e-12	SMART
FN3	1637	1721	1.7e-13	SMART
low complexity region	1754	1767	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106460
AA Change: V1509E

PolyPhen 2 Score 0.019 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000102068
Gene: ENSMUSG00000020758
AA Change: V1509E

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
FN3	1512	1593	1.79e-12	SMART
FN3	1625	1709	1.7e-13	SMART
low complexity region	1742	1755	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106461
AA Change: V1521E

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000102069
Gene: ENSMUSG00000020758
AA Change: V1521E

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
low complexity region	1413	1425	N/A	INTRINSIC
FN3	1524	1605	1.79e-12	SMART
FN3	1637	1721	1.7e-13	SMART
low complexity region	1754	1767	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000169928
AA Change: V1505E

PolyPhen 2 Score 0.047 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000127604
Gene: ENSMUSG00000020758
AA Change: V1505E

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	712	1.32e-28	SMART
low complexity region	715	732	N/A	INTRINSIC
transmembrane domain	737	756	N/A	INTRINSIC
Calx_beta	980	1085	3.13e-35	SMART
FN3	1125	1203	3.15e-8	SMART
FN3	1218	1305	6.29e-8	SMART
low complexity region	1324	1332	N/A	INTRINSIC
FN3	1508	1589	1.79e-12	SMART
FN3	1621	1705	1.7e-13	SMART
low complexity region	1738	1751	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

MGI Phenotype

FUNCTION: Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted null mutations die shortly after birth with extensive detachment of the epidermis and other squamus epithelia. Stratified tissues lack hemidesmosomes and simple epithelia are also defective in adherence. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb5	A	G	12: 118,918,164	L610P	probably damaging	Het
Acta2	G	A	19: 34,252,531	T8I	probably benign	Het
Asphd2	A	G	5: 112,391,941	W9R	probably damaging	Het
Blvrb	T	A	7: 27,465,793	H179Q	probably damaging	Het
Capn13	A	T	17: 73,345,137		probably null	Het
Casp6	A	T	3: 129,912,163	M160L	probably benign	Het
Chordc1	A	G	9: 18,304,372	E140G	probably benign	Het
Col24a1	A	T	3: 145,538,687	Y1572F	possibly damaging	Het
Cul9	A	G	17: 46,541,732	S235P	probably damaging	Het
Cyp2u1	A	T	3: 131,297,953	M306K	probably damaging	Het
Dhx33	A	G	11: 70,999,473	L240P	probably damaging	Het
Dna2	T	A	10: 62,960,275	D494E	probably benign	Het
Dnah7c	C	T	1: 46,632,310	R1620C	probably damaging	Het
Dpp8	G	A	9: 65,054,958	V427M	probably benign	Het
Dpt	G	A	1: 164,796,831	G34S	unknown	Het
Emb	T	A	13: 117,264,510	N198K	probably damaging	Het
Entpd5	T	G	12: 84,396,708	H62P	probably damaging	Het
Ep300	A	C	15: 81,621,152	M658L	unknown	Het
Epb41l4a	T	A	18: 33,797,451	D651V	probably damaging	Het
Epha7	C	T	4: 28,871,937	S422L	probably benign	Het
Ephb2	A	T	4: 136,771,108	V220E	probably damaging	Het
Fam187a	T	A	11: 102,886,048	L226H	possibly damaging	Het
Fbxo42	G	A	4: 141,199,818	A470T	probably benign	Het
Fcgrt	C	T	7: 45,095,251	W264*	probably null	Het
Flt3	A	C	5: 147,349,576	H733Q	probably benign	Het
Gabrr2	A	G	4: 33,082,560	D228G	probably damaging	Het
Gars	T	C	6: 55,077,750	S681P	probably damaging	Het
Gata2	T	C	6: 88,200,408	V140A	possibly damaging	Het
Gm8232	A	T	14: 44,434,927	N100I		Het
Grik4	A	G	9: 42,688,071	C37R	probably damaging	Het
Grm8	T	C	6: 27,618,679	E388G	probably damaging	Het
Hivep3	CGG	CG	4: 120,097,911		probably null	Het
Igsf9b	C	T	9: 27,323,312	T491I	probably damaging	Het
Impa1	C	T	3: 10,324,087	V105I	probably damaging	Het
Inf2	A	G	12: 112,601,337	T134A	probably damaging	Het
Iws1	T	A	18: 32,089,487	D623E	probably benign	Het
Lhfpl5	T	C	17: 28,576,331	S111P	possibly damaging	Het
Lyzl1	T	C	18: 4,169,244	C83R	probably damaging	Het
Madd	G	A	2: 91,169,710	T617M	possibly damaging	Het
Magi2	A	G	5: 20,228,385	T163A	probably damaging	Het
Mdn1	C	A	4: 32,694,599	H1107Q	probably damaging	Het
Mfsd14b	A	T	13: 65,066,777	Y454N	probably benign	Het
Mrgprb3	T	A	7: 48,643,114	I230F	probably benign	Het
Mroh2b	C	T	15: 4,945,023	L1162F	probably damaging	Het
Olfr1019	A	G	2: 85,841,039	F251L	probably benign	Het
Olfr170	T	C	16: 19,606,272	Y131C	probably damaging	Het
Olfr266	G	T	3: 106,822,021	H179Q	probably damaging	Het
Olfr311	A	G	11: 58,841,500	I129V	probably benign	Het
Olfr560	C	T	7: 102,753,745	M61I	probably benign	Het
Olfr631	T	C	7: 103,928,868	L15P	probably damaging	Het
Pclo	T	C	5: 14,679,036	L2636P	unknown	Het
Pfkm	G	A	15: 98,121,310	A181T	probably damaging	Het
Pik3r1	C	T	13: 101,702,838	A169T	probably benign	Het
R3hdml	T	A	2: 163,495,768	M114K	probably damaging	Het
Robo1	C	A	16: 73,024,301	R1310S	probably benign	Het
Scnm1	A	T	3: 95,132,875	N115K	probably benign	Het
Sfn	A	G	4: 133,601,237	V178A	probably damaging	Het
Shank2	C	T	7: 144,091,779	T366I	possibly damaging	Het
Siah1a	T	C	8: 86,725,325	D177G	probably damaging	Het
Slc37a2	A	C	9: 37,237,328	I286S	possibly damaging	Het
Smarce1	T	C	11: 99,228,292	T12A	probably benign	Het
Spata31d1c	T	A	13: 65,035,840	S399T	probably benign	Het
Specc1	A	G	11: 62,211,680	S942G	possibly damaging	Het
Syt13	T	C	2: 92,943,133	F164S	probably benign	Het
Topbp1	A	T	9: 103,332,706	T851S	probably benign	Het
Ttn	A	T	2: 76,969,671	S398T	unknown	Het
Vmn1r12	T	C	6: 57,159,536	V206A	probably damaging	Het
Vmn1r224	G	A	17: 20,419,959	W266*	probably null	Het
Vmn2r68	T	C	7: 85,233,908	D212G	probably benign	Het
Vps13b	G	T	15: 35,770,646	K2078N	probably damaging	Het
Zfp251	A	C	15: 76,854,357	F179V	possibly damaging	Het

Other mutations in Itgb4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01360:Itgb4	APN	11	115990940	missense	probably damaging	1.00
IGL01391:Itgb4	APN	11	115990920	missense	probably damaging	1.00
IGL01431:Itgb4	APN	11	116006457	splice site	probably benign
IGL01750:Itgb4	APN	11	115988926	missense	probably damaging	0.99
IGL01752:Itgb4	APN	11	115988926	missense	probably damaging	0.99
IGL01756:Itgb4	APN	11	115988926	missense	probably damaging	0.99
IGL01766:Itgb4	APN	11	115988926	missense	probably damaging	0.99
IGL01769:Itgb4	APN	11	115988926	missense	probably damaging	0.99
IGL02188:Itgb4	APN	11	116003387	missense	probably benign	0.08
IGL02262:Itgb4	APN	11	115988926	missense	probably damaging	0.99
IGL02293:Itgb4	APN	11	115988926	missense	probably damaging	0.99
IGL02318:Itgb4	APN	11	115988926	missense	probably damaging	0.99
IGL02319:Itgb4	APN	11	115988926	missense	probably damaging	0.99
IGL02338:Itgb4	APN	11	116007969	missense	probably damaging	1.00
IGL02734:Itgb4	APN	11	116005966	missense	probably benign
IGL02879:Itgb4	APN	11	115994352	missense	probably benign	0.05
IGL02889:Itgb4	APN	11	115988905	missense	probably damaging	1.00
IGL03183:Itgb4	APN	11	115988724	missense	probably damaging	1.00
IGL03054:Itgb4	UTSW	11	116000340	nonsense	probably null
R0021:Itgb4	UTSW	11	115979627	missense	possibly damaging	0.95
R0092:Itgb4	UTSW	11	115979124	missense	probably damaging	1.00
R0305:Itgb4	UTSW	11	115979412	missense	probably damaging	1.00
R0408:Itgb4	UTSW	11	116007602	missense	probably damaging	0.99
R0465:Itgb4	UTSW	11	115979756	missense	probably damaging	1.00
R0499:Itgb4	UTSW	11	115979695	missense	probably benign	0.00
R0535:Itgb4	UTSW	11	115991009	missense	possibly damaging	0.86
R0571:Itgb4	UTSW	11	115979768	missense	possibly damaging	0.94
R0613:Itgb4	UTSW	11	115993342	missense	probably damaging	0.98
R0838:Itgb4	UTSW	11	115998162	intron	probably benign
R1381:Itgb4	UTSW	11	115994337	missense	probably benign	0.00
R1451:Itgb4	UTSW	11	115990884	missense	probably damaging	1.00
R1459:Itgb4	UTSW	11	115979111	missense	probably benign	0.42
R1460:Itgb4	UTSW	11	115984164	missense	probably damaging	0.96
R1473:Itgb4	UTSW	11	115984047	missense	probably benign	0.01
R1484:Itgb4	UTSW	11	115999799	missense	probably benign	0.01
R1593:Itgb4	UTSW	11	115980991	missense	probably damaging	1.00
R1623:Itgb4	UTSW	11	115991316	nonsense	probably null
R1633:Itgb4	UTSW	11	116007760	missense	probably damaging	1.00
R1642:Itgb4	UTSW	11	116007357	missense	probably damaging	1.00
R1669:Itgb4	UTSW	11	115991330	missense	probably benign	0.07
R1713:Itgb4	UTSW	11	116003489	missense	probably damaging	1.00
R1732:Itgb4	UTSW	11	115988918	missense	probably damaging	1.00
R1791:Itgb4	UTSW	11	115988520	missense	probably damaging	1.00
R1847:Itgb4	UTSW	11	115983764	missense	probably benign	0.31
R1902:Itgb4	UTSW	11	115980738	missense	probably damaging	0.98
R1945:Itgb4	UTSW	11	115993453	nonsense	probably null
R2102:Itgb4	UTSW	11	116005735	missense	probably benign	0.23
R2184:Itgb4	UTSW	11	115979624	missense	probably damaging	0.96
R2334:Itgb4	UTSW	11	115993435	missense	probably damaging	1.00
R2401:Itgb4	UTSW	11	116006563	missense	possibly damaging	0.67
R3743:Itgb4	UTSW	11	116003670	missense	probably damaging	1.00
R3938:Itgb4	UTSW	11	116005926	missense	possibly damaging	0.92
R4134:Itgb4	UTSW	11	116006470	missense	probably benign	0.03
R4280:Itgb4	UTSW	11	115990935	missense	probably damaging	1.00
R4342:Itgb4	UTSW	11	115988729	missense	probably benign	0.01
R4434:Itgb4	UTSW	11	115999814	missense	probably benign	0.10
R4505:Itgb4	UTSW	11	115983261	splice site	silent
R4585:Itgb4	UTSW	11	115993325	missense	probably damaging	1.00
R4586:Itgb4	UTSW	11	115993325	missense	probably damaging	1.00
R4601:Itgb4	UTSW	11	116005722	missense	probably damaging	1.00
R4921:Itgb4	UTSW	11	116006605	missense	probably benign	0.12
R4962:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5027:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5029:Itgb4	UTSW	11	115988591	intron	probably benign
R5084:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5085:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5124:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5125:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5150:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5175:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5176:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5179:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5207:Itgb4	UTSW	11	116006539	missense	probably damaging	1.00
R5263:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5264:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5334:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5337:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5344:Itgb4	UTSW	11	115989749	missense	probably null	0.92
R5391:Itgb4	UTSW	11	115985068	missense	probably benign	0.05
R5437:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5440:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5653:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5654:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5655:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5772:Itgb4	UTSW	11	115988432	intron	probably benign
R5812:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5813:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5814:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5863:Itgb4	UTSW	11	115990922	missense	probably damaging	1.00
R5864:Itgb4	UTSW	11	115990922	missense	probably damaging	1.00
R5865:Itgb4	UTSW	11	115990922	missense	probably damaging	1.00
R5951:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5954:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R5982:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R6043:Itgb4	UTSW	11	115979386	missense	probably benign	0.30
R6133:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R6134:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R6135:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R6169:Itgb4	UTSW	11	115994276	missense	probably damaging	0.98
R6172:Itgb4	UTSW	11	116000411	missense	probably benign	0.23
R6255:Itgb4	UTSW	11	115998137	missense	possibly damaging	0.83
R6258:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R6259:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R6260:Itgb4	UTSW	11	115984157	missense	probably benign	0.00
R6612:Itgb4	UTSW	11	115984071	missense	probably benign	0.00
R7037:Itgb4	UTSW	11	116005565	nonsense	probably null
R7371:Itgb4	UTSW	11	115998080	missense	probably benign	0.29
R7659:Itgb4	UTSW	11	115979731	missense	probably damaging	1.00
R7759:Itgb4	UTSW	11	116003710	missense	possibly damaging	0.92
R7804:Itgb4	UTSW	11	116003684	missense	probably damaging	1.00
R7832:Itgb4	UTSW	11	116000261	missense	probably damaging	1.00
R7842:Itgb4	UTSW	11	115982705	missense	probably benign	0.18
R7915:Itgb4	UTSW	11	116000261	missense	probably damaging	1.00
R7925:Itgb4	UTSW	11	115982705	missense	probably benign	0.18
R8004:Itgb4	UTSW	11	115982705	missense	not run
X0062:Itgb4	UTSW	11	115993452	missense	probably damaging	1.00
Z1176:Itgb4	UTSW	11	116006520	missense	not run
Z1177:Itgb4	UTSW	11	115986811	missense	not run
Z1177:Itgb4	UTSW	11	115998058	missense	not run

Predicted Primers

PCR Primer
(F):5'- GCTGAAGCCTAACACATCTGC -3'
(R):5'- TGAGACAAGACATGACAGCC -3'

Sequencing Primer
(F):5'- CCTCCTGGGGTATAAAGCAAAAG -3'
(R):5'- TGACAGCCAACAGTCACTATG -3'

Posted On

2019-10-24