Mutagenetix > Incidental Mutation

Incidental Mutation 'R7605:Mroh2b'

588266

Institutional Source

Beutler Lab

Gene Symbol

Mroh2b

Ensembl Gene

ENSMUSG00000022155

Gene Name

maestro heat-like repeat family member 2B

Synonyms

4930455B06Rik, Heatr7b2

MMRRC Submission

045675-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R7605 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

4928219-4991687 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 4974505 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Phenylalanine at position 1162 (L1162F)

Ref Sequence

ENSEMBL: ENSMUSP00000036148 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000045736]

AlphaFold

Q7M6Y6

Predicted Effect

probably damaging
Transcript: ENSMUST00000045736
AA Change: L1162F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000036148
Gene: ENSMUSG00000022155
AA Change: L1162F

Domain	Start	End	E-Value	Type
low complexity region	124	135	N/A	INTRINSIC
low complexity region	824	842	N/A	INTRINSIC
SCOP:d1gw5a_	937	1443	7e-15	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb5	A	G	12: 118,881,899 (GRCm39)	L610P	probably damaging	Het
Acta2	G	A	19: 34,229,931 (GRCm39)	T8I	probably benign	Het
Asphd2	A	G	5: 112,539,807 (GRCm39)	W9R	probably damaging	Het
Blvrb	T	A	7: 27,165,218 (GRCm39)	H179Q	probably damaging	Het
Capn13	A	T	17: 73,652,132 (GRCm39)		probably null	Het
Casp6	A	T	3: 129,705,812 (GRCm39)	M160L	probably benign	Het
Chordc1	A	G	9: 18,215,668 (GRCm39)	E140G	probably benign	Het
Col24a1	A	T	3: 145,244,442 (GRCm39)	Y1572F	possibly damaging	Het
Cul9	A	G	17: 46,852,658 (GRCm39)	S235P	probably damaging	Het
Cyp2u1	A	T	3: 131,091,602 (GRCm39)	M306K	probably damaging	Het
Dhx33	A	G	11: 70,890,299 (GRCm39)	L240P	probably damaging	Het
Dna2	T	A	10: 62,796,054 (GRCm39)	D494E	probably benign	Het
Dnah7c	C	T	1: 46,671,470 (GRCm39)	R1620C	probably damaging	Het
Dpp8	G	A	9: 64,962,240 (GRCm39)	V427M	probably benign	Het
Dpt	G	A	1: 164,624,400 (GRCm39)	G34S	unknown	Het
Emb	T	A	13: 117,401,046 (GRCm39)	N198K	probably damaging	Het
Entpd5	T	G	12: 84,443,482 (GRCm39)	H62P	probably damaging	Het
Ep300	A	C	15: 81,505,353 (GRCm39)	M658L	unknown	Het
Epb41l4a	T	A	18: 33,930,504 (GRCm39)	D651V	probably damaging	Het
Epha7	C	T	4: 28,871,937 (GRCm39)	S422L	probably benign	Het
Ephb2	A	T	4: 136,498,419 (GRCm39)	V220E	probably damaging	Het
Fam187a	T	A	11: 102,776,874 (GRCm39)	L226H	possibly damaging	Het
Fbxo42	G	A	4: 140,927,129 (GRCm39)	A470T	probably benign	Het
Fcgrt	C	T	7: 44,744,675 (GRCm39)	W264*	probably null	Het
Flt3	A	C	5: 147,286,386 (GRCm39)	H733Q	probably benign	Het
Gabrr2	A	G	4: 33,082,560 (GRCm39)	D228G	probably damaging	Het
Gars1	T	C	6: 55,054,735 (GRCm39)	S681P	probably damaging	Het
Gata2	T	C	6: 88,177,390 (GRCm39)	V140A	possibly damaging	Het
Gm8232	A	T	14: 44,672,384 (GRCm39)	N100I		Het
Grik4	A	G	9: 42,599,367 (GRCm39)	C37R	probably damaging	Het
Grm8	T	C	6: 27,618,678 (GRCm39)	E388G	probably damaging	Het
Hivep3	CGG	CG	4: 119,955,108 (GRCm39)	1141	probably null	Het
Igsf9b	C	T	9: 27,234,608 (GRCm39)	T491I	probably damaging	Het
Impa1	C	T	3: 10,389,147 (GRCm39)	V105I	probably damaging	Het
Inf2	A	G	12: 112,567,771 (GRCm39)	T134A	probably damaging	Het
Itgb4	T	A	11: 115,897,302 (GRCm39)	V1521E	probably benign	Het
Iws1	T	A	18: 32,222,540 (GRCm39)	D623E	probably benign	Het
Lhfpl5	T	C	17: 28,795,305 (GRCm39)	S111P	possibly damaging	Het
Lyzl1	T	C	18: 4,169,244 (GRCm39)	C83R	probably damaging	Het
Madd	G	A	2: 91,000,055 (GRCm39)	T617M	possibly damaging	Het
Magi2	A	G	5: 20,433,383 (GRCm39)	T163A	probably damaging	Het
Mdn1	C	A	4: 32,694,599 (GRCm39)	H1107Q	probably damaging	Het
Mfsd14b	A	T	13: 65,214,591 (GRCm39)	Y454N	probably benign	Het
Mrgprb3	T	A	7: 48,292,862 (GRCm39)	I230F	probably benign	Het
Or11i1	G	T	3: 106,729,337 (GRCm39)	H179Q	probably damaging	Het
Or2aj5	T	C	16: 19,425,022 (GRCm39)	Y131C	probably damaging	Het
Or51f23b	C	T	7: 102,402,952 (GRCm39)	M61I	probably benign	Het
Or51m1	T	C	7: 103,578,075 (GRCm39)	L15P	probably damaging	Het
Or5ar1	A	G	2: 85,671,383 (GRCm39)	F251L	probably benign	Het
Or9e1	A	G	11: 58,732,326 (GRCm39)	I129V	probably benign	Het
Pclo	T	C	5: 14,729,050 (GRCm39)	L2636P	unknown	Het
Pfkm	G	A	15: 98,019,191 (GRCm39)	A181T	probably damaging	Het
Pik3r1	C	T	13: 101,839,346 (GRCm39)	A169T	probably benign	Het
R3hdml	T	A	2: 163,337,688 (GRCm39)	M114K	probably damaging	Het
Robo1	C	A	16: 72,821,189 (GRCm39)	R1310S	probably benign	Het
Scnm1	A	T	3: 95,040,186 (GRCm39)	N115K	probably benign	Het
Sfn	A	G	4: 133,328,548 (GRCm39)	V178A	probably damaging	Het
Shank2	C	T	7: 143,645,516 (GRCm39)	T366I	possibly damaging	Het
Siah1a	T	C	8: 87,451,953 (GRCm39)	D177G	probably damaging	Het
Slc37a2	A	C	9: 37,148,624 (GRCm39)	I286S	possibly damaging	Het
Smarce1	T	C	11: 99,119,118 (GRCm39)	T12A	probably benign	Het
Spata31d1c	T	A	13: 65,183,654 (GRCm39)	S399T	probably benign	Het
Specc1	A	G	11: 62,102,506 (GRCm39)	S942G	possibly damaging	Het
Syt13	T	C	2: 92,773,478 (GRCm39)	F164S	probably benign	Het
Topbp1	A	T	9: 103,209,905 (GRCm39)	T851S	probably benign	Het
Ttn	A	T	2: 76,800,015 (GRCm39)	S398T	unknown	Het
Vmn1r12	T	C	6: 57,136,521 (GRCm39)	V206A	probably damaging	Het
Vmn1r224	G	A	17: 20,640,221 (GRCm39)	W266*	probably null	Het
Vmn2r68	T	C	7: 84,883,116 (GRCm39)	D212G	probably benign	Het
Vps13b	G	T	15: 35,770,792 (GRCm39)	K2078N	probably damaging	Het
Zfp251	A	C	15: 76,738,557 (GRCm39)	F179V	possibly damaging	Het

Other mutations in Mroh2b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Mroh2b	APN	15	4,928,679 (GRCm39)	missense	probably benign
IGL00507:Mroh2b	APN	15	4,991,609 (GRCm39)	missense	probably damaging	1.00
IGL00548:Mroh2b	APN	15	4,960,798 (GRCm39)	missense	probably benign	0.35
IGL00902:Mroh2b	APN	15	4,944,704 (GRCm39)	missense	probably damaging	1.00
IGL00944:Mroh2b	APN	15	4,980,609 (GRCm39)	splice site	probably benign
IGL00954:Mroh2b	APN	15	4,932,536 (GRCm39)	missense	probably damaging	0.99
IGL01015:Mroh2b	APN	15	4,971,024 (GRCm39)	missense	probably damaging	1.00
IGL01134:Mroh2b	APN	15	4,944,634 (GRCm39)	missense	probably benign	0.00
IGL01337:Mroh2b	APN	15	4,934,506 (GRCm39)	missense	probably benign	0.38
IGL01780:Mroh2b	APN	15	4,941,482 (GRCm39)	missense	probably benign	0.01
IGL01919:Mroh2b	APN	15	4,953,170 (GRCm39)	missense	probably benign	0.10
IGL02069:Mroh2b	APN	15	4,933,806 (GRCm39)	splice site	probably benign
IGL02146:Mroh2b	APN	15	4,980,776 (GRCm39)	splice site	probably null
IGL02221:Mroh2b	APN	15	4,953,123 (GRCm39)	missense	probably damaging	1.00
IGL02281:Mroh2b	APN	15	4,981,745 (GRCm39)	missense	probably benign	0.04
IGL02350:Mroh2b	APN	15	4,941,482 (GRCm39)	missense	probably benign	0.01
IGL02357:Mroh2b	APN	15	4,941,482 (GRCm39)	missense	probably benign	0.01
IGL02401:Mroh2b	APN	15	4,929,983 (GRCm39)	missense	possibly damaging	0.71
IGL02427:Mroh2b	APN	15	4,981,042 (GRCm39)	splice site	probably benign
IGL02432:Mroh2b	APN	15	4,943,668 (GRCm39)	missense	probably benign
IGL02582:Mroh2b	APN	15	4,937,997 (GRCm39)	missense	probably damaging	0.98
IGL02632:Mroh2b	APN	15	4,960,583 (GRCm39)	missense	probably damaging	0.99
IGL02741:Mroh2b	APN	15	4,935,114 (GRCm39)	missense	probably benign
IGL02811:Mroh2b	APN	15	4,944,718 (GRCm39)	missense	possibly damaging	0.55
IGL02826:Mroh2b	APN	15	4,991,630 (GRCm39)	missense	probably damaging	0.99
IGL03412:Mroh2b	APN	15	4,973,854 (GRCm39)	missense	probably benign	0.14
PIT4468001:Mroh2b	UTSW	15	4,942,294 (GRCm39)	missense	probably damaging	1.00
R0024:Mroh2b	UTSW	15	4,955,109 (GRCm39)	missense	probably damaging	1.00
R0333:Mroh2b	UTSW	15	4,960,600 (GRCm39)	missense	probably damaging	1.00
R0433:Mroh2b	UTSW	15	4,971,116 (GRCm39)	missense	probably benign	0.01
R0530:Mroh2b	UTSW	15	4,963,877 (GRCm39)	missense	probably damaging	0.97
R1411:Mroh2b	UTSW	15	4,947,799 (GRCm39)	missense	probably damaging	1.00
R1457:Mroh2b	UTSW	15	4,955,166 (GRCm39)	missense	probably damaging	1.00
R1466:Mroh2b	UTSW	15	4,955,166 (GRCm39)	missense	probably damaging	1.00
R1466:Mroh2b	UTSW	15	4,955,166 (GRCm39)	missense	probably damaging	1.00
R1472:Mroh2b	UTSW	15	4,978,137 (GRCm39)	missense	probably benign	0.00
R1525:Mroh2b	UTSW	15	4,980,612 (GRCm39)	splice site	probably null
R1584:Mroh2b	UTSW	15	4,955,166 (GRCm39)	missense	probably damaging	1.00
R1605:Mroh2b	UTSW	15	4,974,572 (GRCm39)	missense	probably benign	0.08
R1657:Mroh2b	UTSW	15	4,960,525 (GRCm39)	nonsense	probably null
R1671:Mroh2b	UTSW	15	4,980,776 (GRCm39)	splice site	probably null
R1698:Mroh2b	UTSW	15	4,943,622 (GRCm39)	missense	probably benign	0.02
R2002:Mroh2b	UTSW	15	4,955,166 (GRCm39)	missense	probably damaging	1.00
R2005:Mroh2b	UTSW	15	4,946,640 (GRCm39)	missense	probably damaging	1.00
R2077:Mroh2b	UTSW	15	4,974,448 (GRCm39)	missense	probably damaging	1.00
R2179:Mroh2b	UTSW	15	4,950,928 (GRCm39)	critical splice donor site	probably null
R2183:Mroh2b	UTSW	15	4,947,707 (GRCm39)	splice site	probably null
R3713:Mroh2b	UTSW	15	4,973,131 (GRCm39)	missense	probably benign	0.01
R3714:Mroh2b	UTSW	15	4,973,131 (GRCm39)	missense	probably benign	0.01
R3747:Mroh2b	UTSW	15	4,981,728 (GRCm39)	nonsense	probably null
R3748:Mroh2b	UTSW	15	4,981,728 (GRCm39)	nonsense	probably null
R3749:Mroh2b	UTSW	15	4,981,728 (GRCm39)	nonsense	probably null
R3750:Mroh2b	UTSW	15	4,981,728 (GRCm39)	nonsense	probably null
R3792:Mroh2b	UTSW	15	4,953,102 (GRCm39)	missense	probably damaging	1.00
R3872:Mroh2b	UTSW	15	4,954,543 (GRCm39)	nonsense	probably null
R4021:Mroh2b	UTSW	15	4,954,582 (GRCm39)	missense	possibly damaging	0.75
R4329:Mroh2b	UTSW	15	4,960,861 (GRCm39)	missense	probably damaging	0.99
R4456:Mroh2b	UTSW	15	4,977,407 (GRCm39)	missense	probably benign	0.21
R4592:Mroh2b	UTSW	15	4,947,772 (GRCm39)	missense	probably damaging	1.00
R4836:Mroh2b	UTSW	15	4,933,752 (GRCm39)	missense	probably damaging	1.00
R5050:Mroh2b	UTSW	15	4,929,932 (GRCm39)	missense	possibly damaging	0.82
R5230:Mroh2b	UTSW	15	4,971,004 (GRCm39)	missense	probably benign	0.07
R5342:Mroh2b	UTSW	15	4,943,615 (GRCm39)	nonsense	probably null
R5353:Mroh2b	UTSW	15	4,946,660 (GRCm39)	missense	probably damaging	1.00
R5368:Mroh2b	UTSW	15	4,935,054 (GRCm39)	missense	probably damaging	1.00
R5424:Mroh2b	UTSW	15	4,971,094 (GRCm39)	missense	probably damaging	0.98
R5484:Mroh2b	UTSW	15	4,938,463 (GRCm39)	missense	possibly damaging	0.92
R5999:Mroh2b	UTSW	15	4,942,366 (GRCm39)	splice site	probably null
R6046:Mroh2b	UTSW	15	4,980,763 (GRCm39)	missense	probably benign	0.01
R6081:Mroh2b	UTSW	15	4,973,859 (GRCm39)	missense	probably damaging	1.00
R6162:Mroh2b	UTSW	15	4,944,707 (GRCm39)	missense	probably damaging	1.00
R6165:Mroh2b	UTSW	15	4,947,832 (GRCm39)	missense	probably benign	0.23
R6240:Mroh2b	UTSW	15	4,964,126 (GRCm39)	missense	probably benign	0.38
R6487:Mroh2b	UTSW	15	4,976,721 (GRCm39)	missense	probably damaging	1.00
R6539:Mroh2b	UTSW	15	4,935,056 (GRCm39)	missense	probably damaging	1.00
R6616:Mroh2b	UTSW	15	4,982,764 (GRCm39)	missense	probably benign	0.36
R6663:Mroh2b	UTSW	15	4,977,417 (GRCm39)	missense	probably benign	0.21
R6820:Mroh2b	UTSW	15	4,982,756 (GRCm39)	missense	probably damaging	1.00
R6900:Mroh2b	UTSW	15	4,938,469 (GRCm39)	missense	probably benign	0.00
R6990:Mroh2b	UTSW	15	4,942,284 (GRCm39)	missense	possibly damaging	0.55
R7067:Mroh2b	UTSW	15	4,929,986 (GRCm39)	missense	probably benign	0.35
R7092:Mroh2b	UTSW	15	4,964,160 (GRCm39)	missense	possibly damaging	0.92
R7102:Mroh2b	UTSW	15	4,977,485 (GRCm39)	missense	probably benign	0.06
R7264:Mroh2b	UTSW	15	4,950,844 (GRCm39)	missense	possibly damaging	0.81
R7436:Mroh2b	UTSW	15	4,971,036 (GRCm39)	missense	probably benign	0.21
R7462:Mroh2b	UTSW	15	4,938,109 (GRCm39)	missense	probably damaging	1.00
R7529:Mroh2b	UTSW	15	4,978,491 (GRCm39)	missense	probably damaging	1.00
R7575:Mroh2b	UTSW	15	4,964,087 (GRCm39)	missense	probably damaging	1.00
R7579:Mroh2b	UTSW	15	4,960,543 (GRCm39)	missense	probably benign	0.09
R7624:Mroh2b	UTSW	15	4,946,613 (GRCm39)	missense	probably damaging	1.00
R7797:Mroh2b	UTSW	15	4,978,587 (GRCm39)	missense	probably benign	0.36
R7848:Mroh2b	UTSW	15	4,967,861 (GRCm39)	nonsense	probably null
R7952:Mroh2b	UTSW	15	4,980,693 (GRCm39)	missense	probably damaging	1.00
R7995:Mroh2b	UTSW	15	4,950,839 (GRCm39)	nonsense	probably null
R8088:Mroh2b	UTSW	15	4,929,985 (GRCm39)	missense	possibly damaging	0.57
R8207:Mroh2b	UTSW	15	4,967,892 (GRCm39)	missense	possibly damaging	0.95
R8242:Mroh2b	UTSW	15	4,938,522 (GRCm39)	missense	probably benign	0.04
R8248:Mroh2b	UTSW	15	4,960,586 (GRCm39)	missense	probably benign	0.40
R8258:Mroh2b	UTSW	15	4,941,391 (GRCm39)	missense	probably benign	0.01
R8259:Mroh2b	UTSW	15	4,941,391 (GRCm39)	missense	probably benign	0.01
R8304:Mroh2b	UTSW	15	4,955,119 (GRCm39)	missense	probably damaging	0.99
R8316:Mroh2b	UTSW	15	4,980,746 (GRCm39)	nonsense	probably null
R8345:Mroh2b	UTSW	15	4,973,808 (GRCm39)	missense	probably benign	0.09
R8507:Mroh2b	UTSW	15	4,978,572 (GRCm39)	missense	probably damaging	1.00
R8728:Mroh2b	UTSW	15	4,935,122 (GRCm39)	missense	probably damaging	1.00
R8747:Mroh2b	UTSW	15	4,964,782 (GRCm39)	missense	probably damaging	0.99
R8798:Mroh2b	UTSW	15	4,978,191 (GRCm39)	missense	probably damaging	1.00
R8814:Mroh2b	UTSW	15	4,971,107 (GRCm39)	missense	possibly damaging	0.61
R8856:Mroh2b	UTSW	15	4,960,510 (GRCm39)	nonsense	probably null
R8910:Mroh2b	UTSW	15	4,960,855 (GRCm39)	missense	probably benign	0.01
R8913:Mroh2b	UTSW	15	4,947,010 (GRCm39)	intron	probably benign
R8941:Mroh2b	UTSW	15	4,991,606 (GRCm39)	missense	possibly damaging	0.86
R9014:Mroh2b	UTSW	15	4,928,670 (GRCm39)	start codon destroyed	probably null	0.95
R9086:Mroh2b	UTSW	15	4,982,754 (GRCm39)	critical splice acceptor site	probably null
R9101:Mroh2b	UTSW	15	4,929,935 (GRCm39)	missense	probably benign	0.20
R9118:Mroh2b	UTSW	15	4,991,573 (GRCm39)	missense	possibly damaging	0.86
R9393:Mroh2b	UTSW	15	4,980,666 (GRCm39)	missense	probably benign
R9429:Mroh2b	UTSW	15	4,963,907 (GRCm39)	missense	probably damaging	1.00
R9431:Mroh2b	UTSW	15	4,963,952 (GRCm39)	missense	probably damaging	1.00
R9443:Mroh2b	UTSW	15	4,973,821 (GRCm39)	missense	probably damaging	1.00
R9447:Mroh2b	UTSW	15	4,960,823 (GRCm39)	missense	probably damaging	1.00
R9497:Mroh2b	UTSW	15	4,950,845 (GRCm39)	missense	probably damaging	0.98
R9588:Mroh2b	UTSW	15	4,978,130 (GRCm39)	missense	probably benign	0.00
R9631:Mroh2b	UTSW	15	4,946,556 (GRCm39)	missense	probably damaging	0.97
R9686:Mroh2b	UTSW	15	4,974,605 (GRCm39)	missense	probably benign	0.34
R9774:Mroh2b	UTSW	15	4,943,613 (GRCm39)	missense	probably benign	0.08
X0067:Mroh2b	UTSW	15	4,981,073 (GRCm39)	missense	possibly damaging	0.90
Z1177:Mroh2b	UTSW	15	4,934,487 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAGTCCCTGAAAGTCTGGAC -3'
(R):5'- TTTGATACCAGGGCAGAAAGGC -3'

Sequencing Primer
(F):5'- GAAAGTCTGGACCTTTCTGTACAAGC -3'
(R):5'- GAAAGGCACCAAATCTGAGTTTC -3'

Posted On

2019-10-24