Mutagenetix > Incidental Mutation

Incidental Mutation 'R7608:Vmn2r14'

588429

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r14

Ensembl Gene

ENSMUSG00000091059

Gene Name

vomeronasal 2, receptor 14

Synonyms

EG231591

MMRRC Submission

045678-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.526) question?

Stock #

R7608 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

109362822-109372488 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 109369276 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 99 (I99T)

Ref Sequence

ENSEMBL: ENSMUSP00000128015 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170341]

AlphaFold

E9Q759

Predicted Effect

probably benign
Transcript: ENSMUST00000170341
AA Change: I99T

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000128015
Gene: ENSMUSG00000091059
AA Change: I99T

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.3e-31	PFAM
Pfam:NCD3G	507	561	1.1e-17	PFAM
Pfam:7tm_3	594	829	1.2e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (51/51)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb6	A	G	1: 75,154,347 (GRCm39)	F311S	probably benign	Het
Adamts7	C	A	9: 90,055,826 (GRCm39)	T193N	possibly damaging	Het
Akr1b7	A	G	6: 34,397,457 (GRCm39)	N273S	probably damaging	Het
Asz1	G	A	6: 18,077,252 (GRCm39)	T151M	probably damaging	Het
Atosb	A	G	4: 43,036,533 (GRCm39)	L66P	probably damaging	Het
Atrnl1	T	A	19: 57,703,119 (GRCm39)	Y1046N	probably damaging	Het
Cacna2d1	T	C	5: 16,564,022 (GRCm39)	S902P	probably damaging	Het
Casp3	T	A	8: 47,087,368 (GRCm39)	I105K	probably benign	Het
Ccdc146	C	T	5: 21,506,450 (GRCm39)	V664I	probably benign	Het
Cfap61	G	A	2: 145,805,451 (GRCm39)	C267Y	possibly damaging	Het
Dgkz	A	T	2: 91,764,399 (GRCm39)		probably null	Het
Dnah11	G	T	12: 118,104,505 (GRCm39)		probably null	Het
Duox1	C	A	2: 122,156,616 (GRCm39)	Y514*	probably null	Het
Epha7	C	T	4: 28,871,937 (GRCm39)	S422L	probably benign	Het
Fan1	A	T	7: 64,003,979 (GRCm39)		probably null	Het
Fgfrl1	A	T	5: 108,853,211 (GRCm39)	K278M	probably damaging	Het
Fign	T	C	2: 63,809,063 (GRCm39)	I736V	possibly damaging	Het
Galns	A	G	8: 123,318,184 (GRCm39)	F410S	probably benign	Het
Hspbp1	C	A	7: 4,663,821 (GRCm39)	K341N	possibly damaging	Het
Idh3b	A	T	2: 130,122,900 (GRCm39)	S296R	probably damaging	Het
Ifi44	A	G	3: 151,438,045 (GRCm39)	F414S	probably damaging	Het
Kdm3a	T	A	6: 71,577,731 (GRCm39)	M690L	probably benign	Het
Ldaf1	A	G	7: 119,704,011 (GRCm39)	K19E	probably damaging	Het
Lrrc49	C	T	9: 60,510,005 (GRCm39)	G488S	probably null	Het
Mcc	T	C	18: 44,624,294 (GRCm39)	N417S	possibly damaging	Het
Melk	T	A	4: 44,325,571 (GRCm39)		probably null	Het
Mtcl1	T	C	17: 66,650,300 (GRCm39)	R1722G	probably damaging	Het
Or4a79	A	G	2: 89,551,642 (GRCm39)	V271A	probably benign	Het
Or51f23c-ps1	A	G	7: 102,431,132 (GRCm39)	I150V	probably benign	Het
Or5b119	G	A	19: 13,456,956 (GRCm39)	T202I	possibly damaging	Het
Or8d2	T	A	9: 38,759,806 (GRCm39)	V132D	possibly damaging	Het
Pdzrn3	T	C	6: 101,128,713 (GRCm39)	E651G	probably damaging	Het
Pop5	A	G	5: 115,375,931 (GRCm39)		probably benign	Het
Prox1	T	A	1: 189,885,642 (GRCm39)	M602L	probably benign	Het
Prpf39	G	A	12: 65,100,220 (GRCm39)	A298T	probably benign	Het
Scg2	T	C	1: 79,413,898 (GRCm39)	E275G	probably benign	Het
Scn11a	A	T	9: 119,644,379 (GRCm39)		probably null	Het
Slc16a4	A	T	3: 107,210,443 (GRCm39)	Y371F	probably damaging	Het
Slc4a1ap	T	A	5: 31,693,533 (GRCm39)	M489K	possibly damaging	Het
Spata13	A	G	14: 60,929,956 (GRCm39)	N505D	possibly damaging	Het
Tas2r129	A	G	6: 132,928,156 (GRCm39)	N31S	probably damaging	Het
Tnn	A	T	1: 159,915,984 (GRCm39)	Y1508*	probably null	Het
Trim23	T	C	13: 104,328,541 (GRCm39)	V354A	probably benign	Het
Tsc1	A	T	2: 28,548,748 (GRCm39)	T18S	probably benign	Het
Txlnb	A	G	10: 17,691,146 (GRCm39)	K232R	probably damaging	Het
Vmn1r53	C	T	6: 90,201,104 (GRCm39)	M73I	probably benign	Het
Vmn1r67	G	A	7: 10,181,290 (GRCm39)	V185M	possibly damaging	Het
Zfp318	T	G	17: 46,710,935 (GRCm39)	V886G	probably damaging	Het
Zfp760	A	G	17: 21,941,797 (GRCm39)	N324S	probably benign	Het
Zfp819	T	C	7: 43,266,357 (GRCm39)	V280A	probably benign	Het

Other mutations in Vmn2r14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01472:Vmn2r14	APN	5	109,364,180 (GRCm39)	nonsense	probably null
IGL01504:Vmn2r14	APN	5	109,369,285 (GRCm39)	missense	probably benign	0.01
IGL01828:Vmn2r14	APN	5	109,372,443 (GRCm39)	missense	possibly damaging	0.71
IGL02093:Vmn2r14	APN	5	109,368,275 (GRCm39)	missense	possibly damaging	0.94
IGL02103:Vmn2r14	APN	5	109,372,349 (GRCm39)	missense	probably damaging	0.96
IGL02123:Vmn2r14	APN	5	109,367,933 (GRCm39)	missense	probably damaging	1.00
IGL02145:Vmn2r14	APN	5	109,368,454 (GRCm39)	nonsense	probably null
IGL02676:Vmn2r14	APN	5	109,367,882 (GRCm39)	missense	probably benign	0.03
IGL02720:Vmn2r14	APN	5	109,369,305 (GRCm39)	missense	probably damaging	1.00
IGL02877:Vmn2r14	APN	5	109,368,054 (GRCm39)	missense	probably damaging	0.99
IGL02974:Vmn2r14	APN	5	109,369,292 (GRCm39)	missense	possibly damaging	0.55
IGL03151:Vmn2r14	APN	5	109,364,260 (GRCm39)	missense	probably damaging	1.00
IGL03297:Vmn2r14	APN	5	109,363,973 (GRCm39)	missense	probably damaging	1.00
IGL03386:Vmn2r14	APN	5	109,368,350 (GRCm39)	missense	possibly damaging	0.90
IGL03394:Vmn2r14	APN	5	109,367,702 (GRCm39)	missense	probably null	0.83
ANU74:Vmn2r14	UTSW	5	109,366,910 (GRCm39)	missense	probably benign	0.00
R0316:Vmn2r14	UTSW	5	109,366,762 (GRCm39)	missense	probably benign	0.07
R0755:Vmn2r14	UTSW	5	109,364,226 (GRCm39)	missense	possibly damaging	0.81
R1219:Vmn2r14	UTSW	5	109,372,440 (GRCm39)	missense	probably benign	0.17
R1321:Vmn2r14	UTSW	5	109,364,117 (GRCm39)	missense	probably benign	0.08
R1465:Vmn2r14	UTSW	5	109,368,195 (GRCm39)	missense	possibly damaging	0.47
R1465:Vmn2r14	UTSW	5	109,368,195 (GRCm39)	missense	possibly damaging	0.47
R1509:Vmn2r14	UTSW	5	109,363,862 (GRCm39)	missense	probably benign	0.00
R1551:Vmn2r14	UTSW	5	109,369,283 (GRCm39)	missense	probably damaging	1.00
R1628:Vmn2r14	UTSW	5	109,367,838 (GRCm39)	missense	probably benign	0.00
R1668:Vmn2r14	UTSW	5	109,366,913 (GRCm39)	nonsense	probably null
R2013:Vmn2r14	UTSW	5	109,369,109 (GRCm39)	missense	probably benign	0.00
R2201:Vmn2r14	UTSW	5	109,366,698 (GRCm39)	splice site	probably null
R2417:Vmn2r14	UTSW	5	109,372,329 (GRCm39)	missense	probably benign	0.00
R3029:Vmn2r14	UTSW	5	109,363,776 (GRCm39)	missense	probably damaging	1.00
R3120:Vmn2r14	UTSW	5	109,372,431 (GRCm39)	missense	probably null	0.00
R3729:Vmn2r14	UTSW	5	109,364,095 (GRCm39)	missense	probably damaging	1.00
R3762:Vmn2r14	UTSW	5	109,368,033 (GRCm39)	missense	probably benign	0.02
R3943:Vmn2r14	UTSW	5	109,363,930 (GRCm39)	missense	probably damaging	1.00
R3944:Vmn2r14	UTSW	5	109,363,930 (GRCm39)	missense	probably damaging	1.00
R4222:Vmn2r14	UTSW	5	109,364,149 (GRCm39)	missense	probably benign	0.00
R4224:Vmn2r14	UTSW	5	109,364,149 (GRCm39)	missense	probably benign	0.00
R4239:Vmn2r14	UTSW	5	109,364,277 (GRCm39)	critical splice acceptor site	probably null
R4240:Vmn2r14	UTSW	5	109,364,277 (GRCm39)	critical splice acceptor site	probably null
R4782:Vmn2r14	UTSW	5	109,369,370 (GRCm39)	missense	probably benign	0.01
R4832:Vmn2r14	UTSW	5	109,363,976 (GRCm39)	missense	probably damaging	1.00
R4884:Vmn2r14	UTSW	5	109,369,384 (GRCm39)	splice site	probably null
R4896:Vmn2r14	UTSW	5	109,368,246 (GRCm39)	missense	probably benign	0.19
R5004:Vmn2r14	UTSW	5	109,368,246 (GRCm39)	missense	probably benign	0.19
R5117:Vmn2r14	UTSW	5	109,363,961 (GRCm39)	missense	probably benign	0.16
R5285:Vmn2r14	UTSW	5	109,365,442 (GRCm39)	missense	probably damaging	0.98
R5413:Vmn2r14	UTSW	5	109,369,154 (GRCm39)	missense	probably benign	0.29
R5569:Vmn2r14	UTSW	5	109,368,261 (GRCm39)	missense	probably benign	0.44
R5701:Vmn2r14	UTSW	5	109,367,816 (GRCm39)	missense	probably damaging	1.00
R5726:Vmn2r14	UTSW	5	109,365,486 (GRCm39)	missense	possibly damaging	0.95
R5763:Vmn2r14	UTSW	5	109,363,724 (GRCm39)	missense	possibly damaging	0.49
R5872:Vmn2r14	UTSW	5	109,369,222 (GRCm39)	missense	probably benign
R5985:Vmn2r14	UTSW	5	109,368,082 (GRCm39)	missense	possibly damaging	0.89
R6268:Vmn2r14	UTSW	5	109,369,283 (GRCm39)	missense	possibly damaging	0.87
R6273:Vmn2r14	UTSW	5	109,369,133 (GRCm39)	missense	probably benign	0.44
R6409:Vmn2r14	UTSW	5	109,364,096 (GRCm39)	missense	probably benign	0.09
R6944:Vmn2r14	UTSW	5	109,364,140 (GRCm39)	missense	probably benign	0.06
R6944:Vmn2r14	UTSW	5	109,363,925 (GRCm39)	missense	probably benign	0.22
R7740:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R7768:Vmn2r14	UTSW	5	109,368,086 (GRCm39)	missense	probably benign	0.01
R7804:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R7872:Vmn2r14	UTSW	5	109,369,219 (GRCm39)	missense	probably benign	0.02
R7993:Vmn2r14	UTSW	5	109,363,862 (GRCm39)	missense	probably benign	0.00
R8006:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R8007:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R8187:Vmn2r14	UTSW	5	109,368,420 (GRCm39)	missense	probably benign	0.03
R8369:Vmn2r14	UTSW	5	109,369,342 (GRCm39)	missense	probably damaging	1.00
R8463:Vmn2r14	UTSW	5	109,369,340 (GRCm39)	missense	probably benign	0.30
R8968:Vmn2r14	UTSW	5	109,365,533 (GRCm39)	missense	probably benign	0.01
R9008:Vmn2r14	UTSW	5	109,367,893 (GRCm39)	missense	probably benign	0.00
R9030:Vmn2r14	UTSW	5	109,368,054 (GRCm39)	missense	probably damaging	0.99
R9039:Vmn2r14	UTSW	5	109,367,902 (GRCm39)	nonsense	probably null
R9150:Vmn2r14	UTSW	5	109,367,783 (GRCm39)	missense	probably damaging	1.00
R9164:Vmn2r14	UTSW	5	109,364,087 (GRCm39)	missense	probably damaging	1.00
R9216:Vmn2r14	UTSW	5	109,369,112 (GRCm39)	missense	probably benign	0.01
R9225:Vmn2r14	UTSW	5	109,369,288 (GRCm39)	missense	probably damaging	1.00
R9245:Vmn2r14	UTSW	5	109,368,176 (GRCm39)	missense	possibly damaging	0.89
R9342:Vmn2r14	UTSW	5	109,368,428 (GRCm39)	missense	probably damaging	1.00
R9472:Vmn2r14	UTSW	5	109,367,962 (GRCm39)	missense	probably benign	0.00
R9678:Vmn2r14	UTSW	5	109,364,041 (GRCm39)	missense	probably damaging	1.00
R9774:Vmn2r14	UTSW	5	109,369,126 (GRCm39)	missense	probably benign	0.07
Z1177:Vmn2r14	UTSW	5	109,367,741 (GRCm39)	missense	probably benign	0.33

Predicted Primers

PCR Primer
(F):5'- CCCTTGGTGTCCCAGAATTTG -3'
(R):5'- GTAATCTAGTTCAAGGTTGGCATTC -3'

Sequencing Primer
(F):5'- GTCCCAGAATTTGTTGAGAGTTTTAC -3'
(R):5'- GTTCAAGGTTGGCATTCAATAAAAC -3'

Posted On

2019-10-24