Incidental Mutation 'R7609:Olfr25'
ID588495
Institutional Source Beutler Lab
Gene Symbol Olfr25
Ensembl Gene ENSMUSG00000058270
Gene Nameolfactory receptor 25
SynonymsGA_x6K02T2PVTD-32017922-32018863, MOR170-4, MTPCR18
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.057) question?
Stock #R7609 (G1)
Quality Score225.009
Status Validated
Chromosome9
Chromosomal Location38329060-38335889 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to C at 38330224 bp
ZygosityHeterozygous
Amino Acid Change Leucine to Phenylalanine at position 209 (L209F)
Ref Sequence ENSEMBL: ENSMUSP00000150567 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000071449] [ENSMUST00000212354] [ENSMUST00000214155]
Predicted Effect possibly damaging
Transcript: ENSMUST00000071449
AA Change: L212F

PolyPhen 2 Score 0.878 (Sensitivity: 0.82; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000071393
Gene: ENSMUSG00000058270
AA Change: L212F

DomainStartEndE-ValueType
Pfam:7tm_4 34 310 3.1e-46 PFAM
Pfam:7tm_1 44 243 8e-19 PFAM
Predicted Effect possibly damaging
Transcript: ENSMUST00000212354
AA Change: L209F

PolyPhen 2 Score 0.851 (Sensitivity: 0.83; Specificity: 0.93)
Predicted Effect possibly damaging
Transcript: ENSMUST00000214155
AA Change: L209F

PolyPhen 2 Score 0.851 (Sensitivity: 0.83; Specificity: 0.93)
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency 96% (69/72)
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 71 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930563M21Rik T C 9: 55,989,460 Y288C probably benign Het
Acta2 G A 19: 34,252,531 T8I probably benign Het
Agl C A 3: 116,807,279 R24I possibly damaging Het
Aldoart2 A G 12: 55,566,048 T253A probably benign Het
Ankrd39 G A 1: 36,539,465 R160W probably damaging Het
Aspscr1 C T 11: 120,677,522 T57M probably damaging Het
Baz2b A G 2: 59,962,473 L437S probably benign Het
Bcl9l T A 9: 44,505,747 L331Q probably damaging Het
Cdhr4 C A 9: 107,997,283 P546T probably damaging Het
Cfap61 T C 2: 146,112,533 C231R unknown Het
Cnih4 T C 1: 181,155,902 L61P probably damaging Het
Dtx4 A G 19: 12,492,281 Y161H probably damaging Het
Epha7 C T 4: 28,871,937 S422L probably benign Het
Ercc6 A G 14: 32,566,361 H830R probably benign Het
Exoc7 T C 11: 116,289,259 N673S possibly damaging Het
Gm12185 T C 11: 48,916,023 S114G possibly damaging Het
Hsfy2 A T 1: 56,637,151 F76I probably benign Het
Igsf9b A G 9: 27,345,890 D1413G probably benign Het
Klk8 G A 7: 43,802,179 C198Y probably damaging Het
Lama3 G T 18: 12,531,834 probably null Het
Lce3f A T 3: 92,993,096 S75C unknown Het
Ldhal6b T C 17: 5,417,991 M223V probably benign Het
Magoh T C 4: 107,887,212 V126A possibly damaging Het
Mc1r A T 8: 123,408,293 I262F probably damaging Het
Mecom A G 3: 29,956,442 I629T probably damaging Het
Mkrn2os A G 6: 115,586,726 V116A possibly damaging Het
Muc5b T A 7: 141,861,729 V2804D possibly damaging Het
Myo15 T C 11: 60,488,811 V279A Het
Nhlrc2 A G 19: 56,594,896 I600V probably benign Het
Nipbl T C 15: 8,305,872 Q2183R probably benign Het
Obscn C T 11: 59,000,299 E7136K unknown Het
Olfr1118 T C 2: 87,309,509 V260A probably benign Het
Olfr481 T A 7: 108,081,546 F251I probably damaging Het
Olfr970 C T 9: 39,820,287 T216I probably benign Het
Otop3 T C 11: 115,339,720 I141T possibly damaging Het
Pde4dip C A 3: 97,715,565 V1443F possibly damaging Het
Pdgfra T C 5: 75,166,721 V193A probably benign Het
Phf3 T C 1: 30,805,501 Y1459C probably benign Het
Pias4 A T 10: 81,158,026 probably null Het
Plcg2 A G 8: 117,558,113 N159S probably benign Het
Plekha7 T C 7: 116,164,446 D307G probably benign Het
Ptprd T C 4: 76,072,003 K491E probably benign Het
Rbpjl A T 2: 164,405,814 R62S probably benign Het
Rgs11 T C 17: 26,207,441 S245P probably damaging Het
Ripor3 G A 2: 167,984,570 A685V possibly damaging Het
Rnase9 G A 14: 51,039,483 P13S possibly damaging Het
Scin C T 12: 40,124,589 C165Y probably damaging Het
Senp7 T C 16: 56,111,637 W100R probably benign Het
Slc26a2 T C 18: 61,198,460 D633G probably benign Het
Slc4a4 C T 5: 89,135,686 P446S probably damaging Het
Soga3 A T 10: 29,148,228 D380V probably damaging Het
Spag17 T A 3: 100,095,595 C1878* probably null Het
Ssc5d A G 7: 4,927,576 E129G possibly damaging Het
Supt6 T C 11: 78,226,951 N480D probably benign Het
Tbk1 A G 10: 121,552,501 L590S probably damaging Het
Tex45 A T 8: 3,476,057 H70L probably benign Het
Tmem63a T A 1: 180,952,974 probably null Het
Tpsg1 G T 17: 25,373,210 G86V probably damaging Het
Trio A G 15: 27,912,642 S109P unknown Het
Tsga10 A T 1: 37,804,893 probably null Het
Ttn A T 2: 76,795,763 L14984* probably null Het
Tyr T C 7: 87,483,884 T325A probably benign Het
U2surp A T 9: 95,485,679 probably null Het
Uba6 C A 5: 86,147,075 K368N probably benign Het
Upb1 A G 10: 75,436,201 T283A probably benign Het
Urb1 CACTTAC CAC 16: 90,772,573 probably benign Het
Vmn1r31 A G 6: 58,472,470 Y137H probably damaging Het
Wdtc1 T C 4: 133,296,437 K541R probably damaging Het
Wrn A G 8: 33,310,713 I624T possibly damaging Het
Zfp804b T A 5: 6,770,066 D999V possibly damaging Het
Zmym4 T A 4: 126,925,795 H105L probably benign Het
Other mutations in Olfr25
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01363:Olfr25 APN 9 38329703 missense probably benign 0.01
IGL02044:Olfr25 APN 9 38330165 missense probably benign 0.16
IGL02698:Olfr25 APN 9 38330210 missense probably benign 0.03
IGL03084:Olfr25 APN 9 38330217 missense probably damaging 0.97
IGL03355:Olfr25 APN 9 38329656 missense probably benign 0.05
R0453:Olfr25 UTSW 9 38330171 missense probably benign 0.36
R1584:Olfr25 UTSW 9 38330131 missense possibly damaging 0.90
R1707:Olfr25 UTSW 9 38329901 missense probably damaging 0.99
R1719:Olfr25 UTSW 9 38330507 missense probably benign 0.23
R3409:Olfr25 UTSW 9 38330344 missense possibly damaging 0.94
R4810:Olfr25 UTSW 9 38330394 missense probably benign 0.00
R6271:Olfr25 UTSW 9 38330282 missense probably benign 0.09
R6621:Olfr25 UTSW 9 38330462 missense probably damaging 1.00
R7220:Olfr25 UTSW 9 38329750 missense probably damaging 1.00
R7562:Olfr25 UTSW 9 38329943 missense probably damaging 1.00
R7659:Olfr25 UTSW 9 38329716 missense possibly damaging 0.69
X0019:Olfr25 UTSW 9 38329959 missense probably damaging 1.00
X0027:Olfr25 UTSW 9 38329802 missense probably benign 0.22
Predicted Primers PCR Primer
(F):5'- ACCTAGGATCTGTTGTCTGCTG -3'
(R):5'- CCTTGACCTACAGTCTCAGCAG -3'

Sequencing Primer
(F):5'- CATATTTGATAGGGTTTGCTAGTGCC -3'
(R):5'- TGACCTACAGTCTCAGCAGATGATG -3'
Posted On2019-10-24