Mutagenetix > Incidental Mutation

Incidental Mutation 'R7613:Foxn2'

588744

Institutional Source

Beutler Lab

Gene Symbol

Foxn2

Ensembl Gene

ENSMUSG00000034998

Gene Name

forkhead box N2

Synonyms

Fkh19, 6030465J18Rik, HTLF, 3230402J05Rik

MMRRC Submission

045681-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.258) question?

Stock #

R7613 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

88748139-88797961 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 88794311 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 416 (I416T)

Ref Sequence

ENSEMBL: ENSMUSP00000107857 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000112238] [ENSMUST00000141052]

AlphaFold

E9Q7L6

Predicted Effect

possibly damaging
Transcript: ENSMUST00000112238
AA Change: I416T

PolyPhen 2 Score 0.932 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000107857
Gene: ENSMUSG00000034998
AA Change: I416T

Domain	Start	End	E-Value	Type
FH	110	202	7.42e-50	SMART
low complexity region	314	323	N/A	INTRINSIC
low complexity region	348	358	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000141052

SMART Domains

Protein: ENSMUSP00000118378
Gene: ENSMUSG00000034998

Domain	Start	End	E-Value	Type
FH	110	203	2.15e-37	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (52/52)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a forkhead domain binding protein and may function in the transcriptional regulation of the human T-cell leukemia virus long terminal repeat. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acta2	G	A	19: 34,229,931 (GRCm39)	T8I	probably benign	Het
Adgrb2	CTATA	CTATATA	4: 129,915,006 (GRCm39)		probably benign	Het
Adh1	T	A	3: 137,992,592 (GRCm39)	L236*	probably null	Het
Akt2	A	G	7: 27,336,595 (GRCm39)	I448V	probably benign	Het
Anapc5	G	A	5: 122,956,928 (GRCm39)	T117M	probably damaging	Het
Cacna1d	A	T	14: 29,788,120 (GRCm39)	D1605E	probably benign	Het
Celsr2	C	T	3: 108,302,956 (GRCm39)	G2506R	probably damaging	Het
Cenpe	T	A	3: 134,948,063 (GRCm39)	F31L	possibly damaging	Het
Cenpj	G	A	14: 56,764,501 (GRCm39)	R1304*	probably null	Het
Cfap58	A	G	19: 47,970,561 (GRCm39)	D593G	possibly damaging	Het
Crip2	T	C	12: 113,107,777 (GRCm39)		probably null	Het
Crocc2	G	T	1: 93,122,311 (GRCm39)	A735S	possibly damaging	Het
Dnah2	A	G	11: 69,439,816 (GRCm39)		probably null	Het
Dpp8	C	T	9: 64,960,402 (GRCm39)	T311M	possibly damaging	Het
Eps15	T	C	4: 109,186,922 (GRCm39)	S330P	probably damaging	Het
Exoc2	C	T	13: 31,066,255 (GRCm39)	V474I	probably benign	Het
Gbp8	T	C	5: 105,178,880 (GRCm39)	E145G	probably damaging	Het
Gm3676	T	A	14: 41,365,233 (GRCm39)	I141L	probably benign	Het
Gm4131	A	G	14: 62,718,538 (GRCm39)	Y23H	possibly damaging	Het
Gucy1b1	T	A	3: 81,947,054 (GRCm39)	D385V	possibly damaging	Het
Hdac2	T	C	10: 36,865,232 (GRCm39)	S149P	probably damaging	Het
Hivep3	CGG	CG	4: 119,955,108 (GRCm39)	1141	probably null	Het
Igsf9b	A	T	9: 27,245,418 (GRCm39)	R1128S	probably benign	Het
Invs	A	G	4: 48,392,668 (GRCm39)	H294R	probably damaging	Het
Kansl3	G	A	1: 36,382,876 (GRCm39)	S812F	probably damaging	Het
Katnbl1	T	C	2: 112,239,538 (GRCm39)	S246P	probably benign	Het
Kcnt1	A	T	2: 25,791,358 (GRCm39)	H567L	probably benign	Het
Krt33a	A	G	11: 99,902,765 (GRCm39)	I353T	probably damaging	Het
Lrguk	A	T	6: 34,078,683 (GRCm39)	R639S	possibly damaging	Het
Lrrc37	G	T	11: 103,507,116 (GRCm39)	H1617Q	unknown	Het
Mcoln2	A	T	3: 145,881,299 (GRCm39)		probably null	Het
Myo15a	A	G	11: 60,395,978 (GRCm39)	T1438A		Het
Myocd	A	G	11: 65,109,429 (GRCm39)	L114P	probably damaging	Het
Nutm1	T	C	2: 112,079,584 (GRCm39)	N777S	probably benign	Het
Or5b21	A	T	19: 12,839,141 (GRCm39)	M1L	probably benign	Het
Pde4b	A	G	4: 102,112,503 (GRCm39)	E29G	probably damaging	Het
Plekhn1	G	A	4: 156,309,277 (GRCm39)	P210S	probably benign	Het
Por	G	T	5: 135,758,358 (GRCm39)	A112S	probably damaging	Het
Prkcq	T	C	2: 11,304,221 (GRCm39)	F651S	probably damaging	Het
Rnf148	A	C	6: 23,654,979 (GRCm39)	S6A	probably benign	Het
Selenoi	A	G	5: 30,471,926 (GRCm39)	I376V	possibly damaging	Het
Skint6	A	T	4: 113,034,243 (GRCm39)		probably null	Het
Tdrd6	T	C	17: 43,938,817 (GRCm39)	T744A	probably benign	Het
Tet2	T	A	3: 133,172,509 (GRCm39)	T1918S	possibly damaging	Het
Ttc28	C	A	5: 111,371,995 (GRCm39)	L846M	probably damaging	Het
Ubxn2a	A	T	12: 4,933,832 (GRCm39)	V193D	possibly damaging	Het
Umodl1	T	A	17: 31,207,031 (GRCm39)	C807*	probably null	Het
Upf2	T	A	2: 5,978,347 (GRCm39)	S404T	unknown	Het
Urb1	CACTTAC	CAC	16: 90,569,461 (GRCm39)		probably benign	Het
Usp53	A	T	3: 122,743,467 (GRCm39)	S490T	probably benign	Het
Wscd1	T	C	11: 71,650,799 (GRCm39)	L42P	possibly damaging	Het
Xirp2	T	C	2: 67,344,842 (GRCm39)	I2361T	probably benign	Het

Other mutations in Foxn2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00685:Foxn2	APN	17	88,770,305 (GRCm39)	missense	probably benign	0.02
IGL00952:Foxn2	APN	17	88,783,308 (GRCm39)	missense	probably benign	0.03
IGL02232:Foxn2	APN	17	88,770,479 (GRCm39)	missense	probably benign	0.03
IGL02882:Foxn2	APN	17	88,770,375 (GRCm39)	missense	probably damaging	1.00
IGL02974:Foxn2	APN	17	88,770,543 (GRCm39)	missense	probably damaging	1.00
R1170:Foxn2	UTSW	17	88,781,094 (GRCm39)	critical splice acceptor site	probably benign
R1826:Foxn2	UTSW	17	88,794,233 (GRCm39)	missense	possibly damaging	0.87
R3825:Foxn2	UTSW	17	88,791,837 (GRCm39)	missense	probably damaging	0.98
R4761:Foxn2	UTSW	17	88,770,136 (GRCm39)	splice site	probably null
R5914:Foxn2	UTSW	17	88,770,138 (GRCm39)	splice site	probably null
R6735:Foxn2	UTSW	17	88,794,223 (GRCm39)	missense	probably benign
R7461:Foxn2	UTSW	17	88,794,311 (GRCm39)	missense	possibly damaging	0.93
X0026:Foxn2	UTSW	17	88,794,161 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TGAATTCATCCCAAAGAGCAGC -3'
(R):5'- TTAGAAAGAAACAAGTGGCCCC -3'

Sequencing Primer
(F):5'- TTCCACAGCGACGAGGAGAC -3'
(R):5'- ACAAGTGGCCCCCTCCC -3'

Posted On

2019-10-24