Mutagenetix > Incidental Mutation

Incidental Mutation 'R7620:Vmn2r45'

589099

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r45

Ensembl Gene

ENSMUSG00000090662

Gene Name

vomeronasal 2, receptor 45

Synonyms

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.080) question?

Stock #

R7620 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

8470525-8489075 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 8483223 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Stop codon at position 355 (W355*)

Ref Sequence

ENSEMBL: ENSMUSP00000129466 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164845]

AlphaFold

L7N2B5

Predicted Effect

probably null
Transcript: ENSMUST00000164845
AA Change: W355*

SMART Domains

Protein: ENSMUSP00000129466
Gene: ENSMUSG00000090662
AA Change: W355*

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	154	469	4.5e-26	PFAM
Pfam:NCD3G	512	565	6.4e-21	PFAM
Pfam:7tm_3	598	833	2.1e-54	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

98% (43/44)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700007K13Rik	TCTCTGGGGCAGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTCTGGGGCAGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTCTGGGGCGGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTC	TCTCTGGGGCAGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTCTGGGGCGGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTC	2: 28,466,110		probably benign	Het
Adnp2	T	C	18: 80,130,487	T236A	probably damaging	Het
Alkbh7	A	G	17: 56,997,551	Y63C	probably damaging	Het
Ccr9	T	A	9: 123,779,846	C198S	probably damaging	Het
Chil3	T	A	3: 106,160,435	D138V	probably damaging	Het
Cntd1	A	T	11: 101,283,414	E66D	probably benign	Het
Cpz	A	T	5: 35,511,850	N312K	possibly damaging	Het
Crat	A	G	2: 30,408,078	I203T	probably damaging	Het
Cyp2j13	T	A	4: 96,056,662	H410L	probably benign	Het
Cyp39a1	G	A	17: 43,725,653		probably null	Het
Dnah1	A	T	14: 31,303,906	I828N	possibly damaging	Het
Dnah7b	A	G	1: 46,268,634	D3036G	probably damaging	Het
Dnpep	A	G	1: 75,313,448	V295A	probably benign	Het
Fam111a	A	G	19: 12,587,937	D394G	possibly damaging	Het
Fam26f	A	T	10: 34,127,618	C98S	probably damaging	Het
Fat1	A	G	8: 45,009,850	K1235E	possibly damaging	Het
Gabre	C	A	X: 72,270,259	Q311H	unknown	Het
Garem1	T	C	18: 21,129,841	S639G	probably benign	Het
Gm11639	T	A	11: 104,832,143	S1942T	possibly damaging	Het
Gsdma	A	G	11: 98,666,603	T123A	probably benign	Het
Iqcb1	A	G	16: 36,856,410	N369S	probably benign	Het
Lepr	G	T	4: 101,752,073	V286F	probably benign	Het
Lgsn	T	C	1: 31,203,380	M181T	probably benign	Het
Mapk15	T	G	15: 75,998,848	S512A	probably benign	Het
Mcm3ap	A	G	10: 76,470,433	T127A	probably benign	Het
Msto1	A	G	3: 88,911,307	F315L	possibly damaging	Het
Myo5a	T	A	9: 75,164,136	D673E	probably benign	Het
Nup88	G	C	11: 70,969,779	P58R	probably benign	Het
Olfr1267-ps1	A	C	2: 90,085,633	I276R	probably damaging	Het
Olfr672	C	A	7: 104,996,755	V50L	possibly damaging	Het
Ppp1r18	T	C	17: 35,867,299	V22A	probably benign	Het
Psme1	T	A	14: 55,580,340	C101*	probably null	Het
Rapgef4	G	T	2: 72,229,078	C743F	probably damaging	Het
Rictor	C	T	15: 6,772,154	S441L	probably benign	Het
Rnmt	T	A	18: 68,314,034	Y330N	probably damaging	Het
Skint10	T	A	4: 112,715,817	M261L	possibly damaging	Het
Slc35b1	T	C	11: 95,387,865	Y192H	probably damaging	Het
Socs3	A	G	11: 117,967,570	Y221H	probably damaging	Het
Spink12	A	G	18: 44,104,617		probably benign	Het
Sspo	T	C	6: 48,467,086		probably null	Het
Trp73	G	A	4: 154,059,257	Q551*	probably null	Het
Wdr35	A	G	12: 9,016,042	I678V	probably benign	Het

Other mutations in Vmn2r45

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01287:Vmn2r45	APN	7	8485623	missense	probably benign	0.00
IGL01322:Vmn2r45	APN	7	8481333	missense	possibly damaging	0.49
IGL01402:Vmn2r45	APN	7	8481468	missense	probably damaging	0.98
IGL01404:Vmn2r45	APN	7	8481468	missense	probably damaging	0.98
IGL01529:Vmn2r45	APN	7	8483494	missense	probably benign
IGL01596:Vmn2r45	APN	7	8483273	missense	probably damaging	0.98
IGL01646:Vmn2r45	APN	7	8483338	missense	probably benign	0.18
IGL01819:Vmn2r45	APN	7	8485557	missense	probably benign	0.00
IGL02096:Vmn2r45	APN	7	8481444	missense	probably damaging	0.99
IGL02130:Vmn2r45	APN	7	8483557	missense	possibly damaging	0.46
IGL02409:Vmn2r45	APN	7	8485728	missense	probably benign	0.03
IGL02517:Vmn2r45	APN	7	8483186	nonsense	probably null
IGL02633:Vmn2r45	APN	7	8485729	missense	probably benign	0.01
IGL02940:Vmn2r45	APN	7	8472370	missense	probably damaging	0.99
IGL03281:Vmn2r45	APN	7	8483604	missense	probably damaging	1.00
IGL03358:Vmn2r45	APN	7	8471716	missense	probably damaging	1.00
BB004:Vmn2r45	UTSW	7	8483514	missense	probably benign
BB014:Vmn2r45	UTSW	7	8483514	missense	probably benign
R0382:Vmn2r45	UTSW	7	8483099	missense	probably benign	0.06
R0532:Vmn2r45	UTSW	7	8471821	missense	probably damaging	0.99
R0932:Vmn2r45	UTSW	7	8475381	missense	probably damaging	1.00
R1125:Vmn2r45	UTSW	7	8485543	missense	probably benign	0.00
R1580:Vmn2r45	UTSW	7	8471747	missense	possibly damaging	0.95
R1817:Vmn2r45	UTSW	7	8472373	missense	probably damaging	1.00
R1996:Vmn2r45	UTSW	7	8472025	missense	probably damaging	1.00
R2050:Vmn2r45	UTSW	7	8472022	missense	probably damaging	1.00
R2284:Vmn2r45	UTSW	7	8485766	missense	probably benign	0.02
R4019:Vmn2r45	UTSW	7	8471581	nonsense	probably null
R4227:Vmn2r45	UTSW	7	8483278	missense	probably damaging	0.98
R4381:Vmn2r45	UTSW	7	8471913	nonsense	probably null
R4618:Vmn2r45	UTSW	7	8483437	missense	probably benign	0.00
R4624:Vmn2r45	UTSW	7	8481342	missense	probably damaging	1.00
R4704:Vmn2r45	UTSW	7	8483536	nonsense	probably null
R4735:Vmn2r45	UTSW	7	8483473	missense	probably damaging	1.00
R4868:Vmn2r45	UTSW	7	8481481	missense	probably benign	0.06
R4983:Vmn2r45	UTSW	7	8483117	missense	probably damaging	0.99
R5246:Vmn2r45	UTSW	7	8483252	missense	probably benign	0.00
R5430:Vmn2r45	UTSW	7	8483334	nonsense	probably null
R5504:Vmn2r45	UTSW	7	8483177	missense	probably benign	0.03
R5511:Vmn2r45	UTSW	7	8471833	missense	probably benign	0.19
R5745:Vmn2r45	UTSW	7	8483075	missense	probably benign	0.00
R5814:Vmn2r45	UTSW	7	8471476	missense	probably benign	0.00
R6223:Vmn2r45	UTSW	7	8483302	missense	probably benign
R6267:Vmn2r45	UTSW	7	8472208	missense	probably benign	0.26
R6623:Vmn2r45	UTSW	7	8471501	missense	probably benign	0.09
R6999:Vmn2r45	UTSW	7	8483220	missense	probably benign
R7242:Vmn2r45	UTSW	7	8485613	nonsense	probably null
R7491:Vmn2r45	UTSW	7	8481343	missense	probably benign	0.27
R7719:Vmn2r45	UTSW	7	8483461	missense	probably damaging	0.99
R7720:Vmn2r45	UTSW	7	8483461	missense	probably damaging	0.99
R7853:Vmn2r45	UTSW	7	8482988	missense	possibly damaging	0.90
R7873:Vmn2r45	UTSW	7	8483075	missense	probably benign	0.00
R7882:Vmn2r45	UTSW	7	8483410	missense	possibly damaging	0.82
R7927:Vmn2r45	UTSW	7	8483514	missense	probably benign
R8684:Vmn2r45	UTSW	7	8483512	missense	probably damaging	0.99
R8780:Vmn2r45	UTSW	7	8481372	missense	possibly damaging	0.94
R8811:Vmn2r45	UTSW	7	8471882	missense	probably damaging	1.00
R8893:Vmn2r45	UTSW	7	8485620	missense	probably damaging	1.00
R9043:Vmn2r45	UTSW	7	8471905	missense	possibly damaging	0.92
R9208:Vmn2r45	UTSW	7	8483299	missense	probably damaging	1.00
R9327:Vmn2r45	UTSW	7	8471533	missense	probably damaging	1.00
R9586:Vmn2r45	UTSW	7	8483051	missense	probably benign	0.01
R9599:Vmn2r45	UTSW	7	8475458	critical splice acceptor site	probably null
U24488:Vmn2r45	UTSW	7	8472362	missense	probably damaging	1.00
Z1088:Vmn2r45	UTSW	7	8471485	missense	probably benign	0.16

Predicted Primers

PCR Primer
(F):5'- ATTGTCTATTGCCTGATTATCAGCC -3'
(R):5'- CCCATTTTACAGAGAATATGGATCACC -3'

Sequencing Primer
(F):5'- CCTGTTGCAGATTCATCTCATGGAG -3'
(R):5'- GGATCACCACAAAACAATTGAATTTC -3'

Posted On

2019-10-24