Mutagenetix > Incidental Mutation

Incidental Mutation 'R7622:Wdr90'

589224

Institutional Source

Beutler Lab

Gene Symbol

Wdr90

Ensembl Gene

ENSMUSG00000073434

Gene Name

WD repeat domain 90

Synonyms

3230401M21Rik

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.121) question?

Stock #

R7622 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

25844771-25861501 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 25854109 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 837 (F837L)

Ref Sequence

ENSEMBL: ENSMUSP00000078426 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079461] [ENSMUST00000176923]

AlphaFold

Q6ZPG2

Predicted Effect

probably benign
Transcript: ENSMUST00000079461
AA Change: F837L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000078426
Gene: ENSMUSG00000073434
AA Change: F837L

Domain	Start	End	E-Value	Type
low complexity region	32	53	N/A	INTRINSIC
Pfam:DUF667	60	183	5e-26	PFAM
Pfam:DUF667	210	271	3.7e-9	PFAM
low complexity region	381	396	N/A	INTRINSIC
WD40	478	519	5.94e0	SMART
WD40	522	565	3.2e0	SMART
WD40	572	612	3.3e1	SMART
WD40	687	725	1.15e1	SMART
WD40	728	766	5.75e-1	SMART
WD40	768	808	9.24e-4	SMART
WD40	811	850	4.13e0	SMART
WD40	853	892	4.62e-1	SMART
WD40	950	993	1.07e1	SMART
WD40	996	1035	5.75e-1	SMART
WD40	1040	1077	1.58e-2	SMART
WD40	1290	1334	5.23e-3	SMART
WD40	1337	1378	1.27e-1	SMART
WD40	1384	1419	1.83e2	SMART
WD40	1422	1469	3.08e0	SMART
WD40	1472	1509	9.9e0	SMART
WD40	1568	1607	9.02e-7	SMART
WD40	1610	1655	5.75e-1	SMART
WD40	1659	1697	2.98e-1	SMART
WD40	1700	1749	6.14e1	SMART
WD40	1850	1888	1.92e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000176678

Predicted Effect

probably benign
Transcript: ENSMUST00000176923
AA Change: F819L

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000135420
Gene: ENSMUSG00000073434
AA Change: F819L

Domain	Start	End	E-Value	Type
low complexity region	32	53	N/A	INTRINSIC
Pfam:DUF667	60	252	5.1e-14	PFAM
low complexity region	363	378	N/A	INTRINSIC
WD40	460	501	5.94e0	SMART
WD40	504	547	3.2e0	SMART
WD40	554	594	3.3e1	SMART
WD40	669	707	1.15e1	SMART
WD40	710	748	5.75e-1	SMART
WD40	750	790	9.24e-4	SMART
WD40	793	832	4.13e0	SMART
WD40	835	874	4.62e-1	SMART
WD40	932	975	1.07e1	SMART
WD40	978	1017	5.75e-1	SMART
WD40	1022	1059	1.58e-2	SMART
WD40	1272	1316	5.23e-3	SMART
WD40	1319	1360	1.27e-1	SMART
WD40	1366	1401	1.83e2	SMART
WD40	1404	1451	3.08e0	SMART
WD40	1454	1491	9.9e0	SMART
WD40	1550	1589	9.02e-7	SMART
WD40	1592	1637	5.75e-1	SMART
WD40	1641	1679	2.98e-1	SMART
WD40	1682	1731	6.14e1	SMART
WD40	1832	1870	1.92e-1	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

98% (55/56)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932438A13Rik	C	A	3: 36,948,413	C1502*	probably null	Het
Adgrd1	G	A	5: 129,139,624	D384N	probably benign	Het
Aqp8	T	C	7: 123,466,660	F226S	possibly damaging	Het
Arl6ip6	A	G	2: 53,217,327	Y207C	probably damaging	Het
Cbfa2t2	A	G	2: 154,500,445	E27G	possibly damaging	Het
Cdc42bpb	T	G	12: 111,294,772	E201A	unknown	Het
Cfap54	A	C	10: 92,956,944	V1769G	unknown	Het
Dmxl2	C	T	9: 54,472,218	G7S	probably damaging	Het
Dnah6	G	T	6: 73,124,759	F1927L	possibly damaging	Het
Dnajc6	A	G	4: 101,640,491	E942G	probably damaging	Het
Fam170a	A	G	18: 50,282,902	R324G	probably benign	Het
Gapdhs	G	A	7: 30,739,331	T9I	unknown	Het
Gm10320	T	A	13: 98,489,724	R51*	probably null	Het
Gm19410	A	T	8: 35,810,347	T1776S	possibly damaging	Het
Gm4846	A	T	1: 166,495,872	M94K	possibly damaging	Het
Ighv2-5	G	A	12: 113,685,738	Q32*	probably null	Het
Klc2	T	C	19: 5,111,632	E310G	probably damaging	Het
Krt75	A	G	15: 101,570,272	M309T	probably damaging	Het
Lrp11	G	A	10: 7,590,172	G41R	unknown	Het
Lrrc52	G	T	1: 167,466,095	P207Q	probably benign	Het
Lrrc52	G	T	1: 167,466,096	P207T	probably benign	Het
Lrrk2	A	T	15: 91,812,323	D2438V	probably damaging	Het
Mycbp	C	T	4: 123,905,301	T28M	probably damaging	Het
Myh14	A	G	7: 44,632,422	V804A	probably benign	Het
Myo16	A	G	8: 10,376,238	I332V	unknown	Het
Ncor1	G	T	11: 62,317,968	Q1359K	probably benign	Het
Olfr1055	A	G	2: 86,347,662	Y35H	possibly damaging	Het
Olfr1112	A	T	2: 87,192,250	I188L	probably benign	Het
Olfr50	A	G	2: 36,793,931	K232E	probably benign	Het
Olfr548-ps1	A	G	7: 102,542,623	H229R	probably benign	Het
Oog3	T	C	4: 144,158,319	D349G	probably benign	Het
Pate4	A	G	9: 35,608,299	S32P	possibly damaging	Het
Pcdhb7	A	C	18: 37,342,461	S217R	probably benign	Het
Pde1c	G	A	6: 56,126,925	R580C	probably damaging	Het
Pi4ka	C	A	16: 17,293,977	A1545S		Het
Pign	G	T	1: 105,648,117	T266K	possibly damaging	Het
Pitpnm2	A	G	5: 124,122,027	I1134T	probably benign	Het
Ppfia2	G	A	10: 106,830,659	V409I	possibly damaging	Het
Prr7	A	G	13: 55,472,796	T206A	probably benign	Het
Rnf13	A	T	3: 57,820,534	R212*	probably null	Het
Rwdd2b	T	C	16: 87,434,612	N218S	probably benign	Het
Serpina3m	A	G	12: 104,389,575	D167G	possibly damaging	Het
Slit2	A	T	5: 47,985,205	N56Y	probably damaging	Het
Sprr3	G	T	3: 92,457,285	T84K	probably damaging	Het
Stab2	A	T	10: 86,873,902	H1626Q	possibly damaging	Het
Tex2	C	A	11: 106,546,895	D650Y	unknown	Het
Tmprss6	A	G	15: 78,446,726	S436P	probably benign	Het
Uba2	A	C	7: 34,165,435	F63L	probably damaging	Het
Ubxn2b	C	T	4: 6,214,692	S242L	probably damaging	Het
Vmn1r86	T	G	7: 13,102,758	I64L	probably benign	Het
Vmn2r67	A	T	7: 85,136,454	V781E	probably damaging	Het
Vmn2r80	T	A	10: 79,194,263	F641Y	probably damaging	Het
Wdfy4	A	T	14: 33,078,274	I1965K		Het
Wdr25	G	A	12: 108,992,893	G344S	possibly damaging	Het
Xpo4	A	G	14: 57,597,011	V704A	possibly damaging	Het
Zc3h12d	A	G	10: 7,867,269	K268E	probably damaging	Het

Other mutations in Wdr90

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00418:Wdr90	APN	17	25849364	missense	probably damaging	1.00
IGL01118:Wdr90	APN	17	25854687	missense	probably damaging	1.00
IGL01964:Wdr90	APN	17	25848409	missense	probably benign
IGL02116:Wdr90	APN	17	25859492	missense	probably benign	0.12
IGL02172:Wdr90	APN	17	25850434	missense	probably benign	0.22
IGL02716:Wdr90	APN	17	25857220	missense	probably damaging	0.96
IGL02961:Wdr90	APN	17	25848675	nonsense	probably null
IGL03229:Wdr90	APN	17	25845463	splice site	probably benign
IGL03367:Wdr90	APN	17	25847791	splice site	probably benign
IGL03098:Wdr90	UTSW	17	25859987	intron	probably benign
R0111:Wdr90	UTSW	17	25848444	splice site	probably benign
R0454:Wdr90	UTSW	17	25860049	missense	probably damaging	0.96
R0457:Wdr90	UTSW	17	25860485	missense	probably benign	0.00
R0488:Wdr90	UTSW	17	25848617	missense	probably damaging	1.00
R0622:Wdr90	UTSW	17	25855658	missense	probably damaging	1.00
R0671:Wdr90	UTSW	17	25846393	missense	probably benign	0.04
R0799:Wdr90	UTSW	17	25860130	missense	probably benign	0.38
R1177:Wdr90	UTSW	17	25846054	missense	possibly damaging	0.85
R1460:Wdr90	UTSW	17	25860448	missense	possibly damaging	0.82
R1468:Wdr90	UTSW	17	25854053	missense	probably damaging	1.00
R1468:Wdr90	UTSW	17	25854053	missense	probably damaging	1.00
R1544:Wdr90	UTSW	17	25849310	missense	possibly damaging	0.69
R2057:Wdr90	UTSW	17	25855199	missense	probably benign	0.05
R2087:Wdr90	UTSW	17	25846603	missense	probably damaging	1.00
R2159:Wdr90	UTSW	17	25851741	missense	probably benign
R2208:Wdr90	UTSW	17	25860388	missense	probably damaging	1.00
R2345:Wdr90	UTSW	17	25859162	missense	probably benign	0.05
R2391:Wdr90	UTSW	17	25851455	missense	probably damaging	1.00
R2394:Wdr90	UTSW	17	25851455	missense	probably damaging	1.00
R2520:Wdr90	UTSW	17	25855352	missense	probably damaging	1.00
R3798:Wdr90	UTSW	17	25850498	missense	probably benign	0.01
R3979:Wdr90	UTSW	17	25859278	missense	probably benign	0.00
R4111:Wdr90	UTSW	17	25849368	missense	possibly damaging	0.94
R4326:Wdr90	UTSW	17	25853731	missense	probably benign	0.25
R4459:Wdr90	UTSW	17	25861002	missense	possibly damaging	0.95
R4697:Wdr90	UTSW	17	25855363	missense	probably benign	0.03
R4735:Wdr90	UTSW	17	25859450	missense	probably benign
R4907:Wdr90	UTSW	17	25860650	intron	probably benign
R5070:Wdr90	UTSW	17	25846333	missense	probably damaging	1.00
R5230:Wdr90	UTSW	17	25855303	missense	probably benign	0.01
R5268:Wdr90	UTSW	17	25850845	missense	probably damaging	1.00
R5287:Wdr90	UTSW	17	25861467	utr 5 prime	probably benign
R5382:Wdr90	UTSW	17	25845598	missense	probably damaging	1.00
R5511:Wdr90	UTSW	17	25845021	unclassified	probably benign
R5545:Wdr90	UTSW	17	25845856	missense	probably damaging	1.00
R5707:Wdr90	UTSW	17	25857192	missense	probably benign	0.00
R5973:Wdr90	UTSW	17	25845133	missense	probably damaging	0.99
R5973:Wdr90	UTSW	17	25846407	missense	probably damaging	1.00
R6385:Wdr90	UTSW	17	25848530	missense	probably damaging	1.00
R6481:Wdr90	UTSW	17	25845911	missense	probably damaging	0.99
R7078:Wdr90	UTSW	17	25849649	missense	probably damaging	1.00
R7214:Wdr90	UTSW	17	25845393	missense	probably benign	0.00
R7288:Wdr90	UTSW	17	25846312	missense	probably benign	0.03
R7304:Wdr90	UTSW	17	25851506	missense	probably benign	0.10
R7309:Wdr90	UTSW	17	25860702	missense	probably benign	0.02
R7391:Wdr90	UTSW	17	25846528	missense	probably benign	0.08
R7646:Wdr90	UTSW	17	25860130	missense	probably benign	0.38
R7772:Wdr90	UTSW	17	25861491	start gained	probably benign
R7779:Wdr90	UTSW	17	25846326	missense	probably damaging	1.00
R7780:Wdr90	UTSW	17	25846326	missense	probably damaging	1.00
R7781:Wdr90	UTSW	17	25846326	missense	probably damaging	1.00
R7782:Wdr90	UTSW	17	25846326	missense	probably damaging	1.00
R7812:Wdr90	UTSW	17	25852558	missense	probably damaging	1.00
R7870:Wdr90	UTSW	17	25860539	missense	probably damaging	0.96
R7911:Wdr90	UTSW	17	25850749	missense	probably benign	0.00
R8126:Wdr90	UTSW	17	25848977	missense	possibly damaging	0.48
R8260:Wdr90	UTSW	17	25845167	missense	probably damaging	1.00
R8315:Wdr90	UTSW	17	25845425	missense	probably benign	0.21
R8919:Wdr90	UTSW	17	25857172	missense
R8938:Wdr90	UTSW	17	25857172	missense
R8940:Wdr90	UTSW	17	25857172	missense
R9038:Wdr90	UTSW	17	25857226	missense
R9079:Wdr90	UTSW	17	25857429	missense
R9401:Wdr90	UTSW	17	25845776	small insertion	probably benign
R9471:Wdr90	UTSW	17	25861041	missense	possibly damaging	0.72
R9642:Wdr90	UTSW	17	25853720	critical splice donor site	probably null
X0064:Wdr90	UTSW	17	25848563	missense	probably damaging	1.00
Z1176:Wdr90	UTSW	17	25860496	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GCTTTCCTGCAAAGGATCCTC -3'
(R):5'- GGCACAATTGTCCCTTGAGG -3'

Sequencing Primer
(F):5'- AGGATCCTCCCCACATGG -3'
(R):5'- GCTGGCCTTGAACTCAGAAATCTG -3'

Posted On

2019-10-24