Mutagenetix > Incidental Mutation

Incidental Mutation 'R7624:Zfp366'

589347

Institutional Source

Beutler Lab

Gene Symbol

Zfp366

Ensembl Gene

ENSMUSG00000050919

Gene Name

zinc finger protein 366

Synonyms

DC-SCRIPT

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.274) question?

Stock #

R7624 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

99184823-99250656 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 99246296 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 656 (T656A)

Ref Sequence

ENSEMBL: ENSMUSP00000060040 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056558]

AlphaFold

Q6NS86

Predicted Effect

probably benign
Transcript: ENSMUST00000056558
AA Change: T656A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000060040
Gene: ENSMUSG00000050919
AA Change: T656A

Domain	Start	End	E-Value	Type
low complexity region	159	175	N/A	INTRINSIC
low complexity region	183	196	N/A	INTRINSIC
ZnF_C2H2	250	272	1.18e-2	SMART
ZnF_C2H2	278	300	4.05e-1	SMART
ZnF_C2H2	306	328	1.79e-2	SMART
ZnF_C2H2	334	356	1.53e-1	SMART
ZnF_C2H2	362	384	1.89e-1	SMART
ZnF_C2H2	390	412	1.22e-4	SMART
ZnF_C2H2	418	440	1.36e-2	SMART
ZnF_C2H2	446	468	1.1e-2	SMART
ZnF_C2H2	474	496	8.34e-3	SMART
ZnF_C2H2	502	524	5.42e-2	SMART
ZnF_C2H2	530	553	2.4e-3	SMART
low complexity region	615	623	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

99% (69/70)

MGI Phenotype

PHENOTYPE: Mice homozygous for an ENU-induced mutation exhibit perimembranous and muscular ventricular septal defects (VSD), and overriding aorta. Short snout, micrognathia, micropthalmia, hypoplastic thymus, and hydronephrosis are also observed. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atm	C	A	9: 53,454,768	R2533L	probably damaging	Het
Caprin1	T	A	2: 103,772,677	M514L	possibly damaging	Het
Ccdc78	A	G	17: 25,787,152	E98G	probably damaging	Het
Cmya5	A	T	13: 93,090,357	M2741K	possibly damaging	Het
Cnot1	T	G	8: 95,751,819	R959S	probably damaging	Het
Col12a1	G	T	9: 79,645,794		probably null	Het
Col4a3	A	G	1: 82,718,884	T177A	probably benign	Het
Cpa3	T	C	3: 20,225,143	N211D	possibly damaging	Het
Cryba1	T	C	11: 77,722,717	Y36C	probably damaging	Het
Ctnna1	T	A	18: 35,244,844	I632N	probably benign	Het
D630045J12Rik	T	C	6: 38,149,563	N1504S	probably damaging	Het
Dgkz	C	A	2: 91,942,674	R346L	probably damaging	Het
Dnajc21	G	A	15: 10,461,232	R211C	probably benign	Het
Dnajc21	A	T	15: 10,461,234	I210N	probably damaging	Het
Dscam	T	C	16: 96,610,324	D1784G	probably damaging	Het
Eya3	A	G	4: 132,672,951	T94A	probably benign	Het
Fan1	T	A	7: 64,372,486	N340Y	probably damaging	Het
Fat3	T	C	9: 15,959,869	D3742G	possibly damaging	Het
Ficd	T	C	5: 113,738,690	F309L	probably benign	Het
Gfra1	A	T	19: 58,238,446	M451K	probably benign	Het
Gm43517	T	A	12: 49,391,178	L318Q		Het
Gsc	A	T	12: 104,473,051	V22E	possibly damaging	Het
Il4ra	T	C	7: 125,569,108	Y100H	probably damaging	Het
Ilf3	T	C	9: 21,398,044	S486P	probably benign	Het
Itga10	T	A	3: 96,652,953	H594Q	probably benign	Het
Kcnf1	T	C	12: 17,176,136	D28G	probably benign	Het
Kiss1	A	G	1: 133,329,422	K53R	possibly damaging	Het
Lcn5	A	G	2: 25,661,414	I182V	probably benign	Het
Lcorl	T	C	5: 45,701,965	D269G	probably benign	Het
Letm2	A	T	8: 25,592,537	C174*	probably null	Het
Met	T	G	6: 17,558,835	V1154G	probably damaging	Het
Mindy3	A	G	2: 12,419,189	S22P	probably benign	Het
Mroh2b	G	A	15: 4,917,131	R471Q	probably damaging	Het
Nfe2l3	T	C	6: 51,458,272	L604P	probably damaging	Het
Olfr1044	A	T	2: 86,171,220	I199N	possibly damaging	Het
Olfr1145	A	G	2: 87,810,339	Q173R	probably damaging	Het
Olfr186	T	A	16: 59,027,019	Q296L	possibly damaging	Het
Olfr294	A	G	7: 86,616,561	F28S	possibly damaging	Het
Olfr824	A	C	10: 130,126,586	I157S	probably damaging	Het
Olfr918	C	T	9: 38,672,623	V274I	probably benign	Het
Pcdha9	T	A	18: 36,999,796	Y639*	probably null	Het
Pom121l2	A	T	13: 21,983,529	I657L	probably damaging	Het
Prr14l	T	A	5: 32,829,623	T843S	possibly damaging	Het
Rln1	A	T	19: 29,332,099	N93K	probably damaging	Het
Rpl3l	A	T	17: 24,732,427	M101L	probably benign	Het
Rtkn	A	G	6: 83,152,177	Y551C	probably benign	Het
Rundc1	G	C	11: 101,433,479	R337P	possibly damaging	Het
Scarf1	T	A	11: 75,514,416		probably null	Het
Serpinb1b	T	A	13: 33,091,639		probably null	Het
Sgsm1	A	T	5: 113,274,335	C484*	probably null	Het
Slc22a19	T	A	19: 7,673,303	K547*	probably null	Het
Slc22a19	T	C	19: 7,693,818	T177A	probably benign	Het
Slc6a5	A	C	7: 49,941,866	K526N	probably benign	Het
Sqle	G	A	15: 59,330,754	R519Q	probably benign	Het
Stard9	T	C	2: 120,688,146	I517T	probably benign	Het
Taf15	A	T	11: 83,505,023	D518V	unknown	Het
Tdgf1	C	T	9: 110,945,949		probably benign	Het
Tenm4	A	G	7: 96,895,985	M2440V	possibly damaging	Het
Tm6sf1	A	G	7: 81,868,710	M122V	possibly damaging	Het
Tmprss9	T	A	10: 80,892,219	S548T	possibly damaging	Het
Trim62	A	T	4: 128,883,678		probably benign	Het
Ugt2b34	C	T	5: 86,891,282	V507M	possibly damaging	Het
Usp16	T	A	16: 87,476,805	M423K	probably benign	Het
Vmn1r199	T	A	13: 22,382,736	S67T	probably benign	Het
Vmn1r62	A	T	7: 5,675,601	M94L	probably benign	Het
Wnk4	G	T	11: 101,264,354	E364*	probably null	Het
Xpo1	T	A	11: 23,282,584	I368N	probably damaging	Het
Zfp934	T	C	13: 62,518,524	M113V	probably benign	Het
Zkscan2	A	G	7: 123,498,771	V134A	probably damaging	Het

Other mutations in Zfp366

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00585:Zfp366	APN	13	99246572	utr 3 prime	probably benign
IGL01626:Zfp366	APN	13	99228412	missense	probably damaging	0.99
IGL02227:Zfp366	APN	13	99234188	missense	possibly damaging	0.85
IGL03074:Zfp366	APN	13	99246405	missense	probably benign
R0126:Zfp366	UTSW	13	99228621	missense	probably benign	0.14
R0145:Zfp366	UTSW	13	99229540	missense	probably damaging	1.00
R0234:Zfp366	UTSW	13	99234260	missense	probably damaging	1.00
R0234:Zfp366	UTSW	13	99234260	missense	probably damaging	1.00
R0376:Zfp366	UTSW	13	99234251	missense	probably benign	0.00
R0537:Zfp366	UTSW	13	99229278	missense	probably damaging	1.00
R0637:Zfp366	UTSW	13	99228966	missense	probably damaging	0.99
R0838:Zfp366	UTSW	13	99228610	missense	possibly damaging	0.73
R1386:Zfp366	UTSW	13	99246555	missense	probably damaging	0.98
R1422:Zfp366	UTSW	13	99229296	missense	probably damaging	1.00
R1669:Zfp366	UTSW	13	99229561	missense	probably damaging	0.99
R1839:Zfp366	UTSW	13	99228492	missense	probably damaging	0.98
R3751:Zfp366	UTSW	13	99228844	missense	probably damaging	1.00
R4782:Zfp366	UTSW	13	99246483	missense	probably damaging	1.00
R4908:Zfp366	UTSW	13	99234101	missense	possibly damaging	0.68
R4992:Zfp366	UTSW	13	99229495	missense	possibly damaging	0.62
R5040:Zfp366	UTSW	13	99228367	missense	probably damaging	1.00
R5086:Zfp366	UTSW	13	99228943	missense	probably benign	0.00
R5186:Zfp366	UTSW	13	99246168	missense	probably benign	0.00
R5249:Zfp366	UTSW	13	99229609	missense	probably damaging	1.00
R5450:Zfp366	UTSW	13	99229585	missense	probably damaging	1.00
R6838:Zfp366	UTSW	13	99228507	missense	possibly damaging	0.93
R6838:Zfp366	UTSW	13	99246177	missense	possibly damaging	0.83
R7250:Zfp366	UTSW	13	99229568	missense	probably damaging	1.00
R7378:Zfp366	UTSW	13	99229515	missense	probably damaging	1.00
R7571:Zfp366	UTSW	13	99246387	missense	probably benign	0.03
R7653:Zfp366	UTSW	13	99229201	missense	probably damaging	1.00
R8367:Zfp366	UTSW	13	99244043	missense	possibly damaging	0.95
R9510:Zfp366	UTSW	13	99229366	missense	probably damaging	1.00
R9658:Zfp366	UTSW	13	99228927	missense	probably benign	0.13
R9734:Zfp366	UTSW	13	99228844	missense	probably damaging	1.00
Z1176:Zfp366	UTSW	13	99246350	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- AGGAACCATTTGACCTCTCCC -3'
(R):5'- GCCCTCATCTCTGTGCTTGAAG -3'

Sequencing Primer
(F):5'- ATTTGACCTCTCCCAGAAGCG -3'
(R):5'- CCTCATCTCTGTGCTTGAAGTATAAG -3'

Posted On

2019-10-24