Mutagenetix > Incidental Mutation

Incidental Mutation 'R7624:Mroh2b'

589348

Institutional Source

Beutler Lab

Gene Symbol

Mroh2b

Ensembl Gene

ENSMUSG00000022155

Gene Name

maestro heat-like repeat family member 2B

Synonyms

4930455B06Rik, Heatr7b2

MMRRC Submission

045689-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.122) question?

Stock #

R7624 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

4898737-4962205 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 4917131 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glutamine at position 471 (R471Q)

Ref Sequence

ENSEMBL: ENSMUSP00000036148 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000045736]

AlphaFold

Q7M6Y6

Predicted Effect

probably damaging
Transcript: ENSMUST00000045736
AA Change: R471Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000036148
Gene: ENSMUSG00000022155
AA Change: R471Q

Domain	Start	End	E-Value	Type
low complexity region	124	135	N/A	INTRINSIC
low complexity region	824	842	N/A	INTRINSIC
SCOP:d1gw5a_	937	1443	7e-15	SMART

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

99% (69/70)

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atm	C	A	9: 53,454,768 (GRCm38)	R2533L	probably damaging	Het
Caprin1	T	A	2: 103,772,677 (GRCm38)	M514L	possibly damaging	Het
Ccdc78	A	G	17: 25,787,152 (GRCm38)	E98G	probably damaging	Het
Cmya5	A	T	13: 93,090,357 (GRCm38)	M2741K	possibly damaging	Het
Cnot1	T	G	8: 95,751,819 (GRCm38)	R959S	probably damaging	Het
Col12a1	G	T	9: 79,645,794 (GRCm38)		probably null	Het
Col4a3	A	G	1: 82,718,884 (GRCm38)	T177A	probably benign	Het
Cpa3	T	C	3: 20,225,143 (GRCm38)	N211D	possibly damaging	Het
Cripto	C	T	9: 110,945,949 (GRCm38)		probably benign	Het
Cryba1	T	C	11: 77,722,717 (GRCm38)	Y36C	probably damaging	Het
Ctnna1	T	A	18: 35,244,844 (GRCm38)	I632N	probably benign	Het
D630045J12Rik	T	C	6: 38,149,563 (GRCm38)	N1504S	probably damaging	Het
Dgkz	C	A	2: 91,942,674 (GRCm38)	R346L	probably damaging	Het
Dnajc21	A	T	15: 10,461,234 (GRCm38)	I210N	probably damaging	Het
Dnajc21	G	A	15: 10,461,232 (GRCm38)	R211C	probably benign	Het
Dscam	T	C	16: 96,610,324 (GRCm38)	D1784G	probably damaging	Het
Eya3	A	G	4: 132,672,951 (GRCm38)	T94A	probably benign	Het
Fan1	T	A	7: 64,372,486 (GRCm38)	N340Y	probably damaging	Het
Fat3	T	C	9: 15,959,869 (GRCm38)	D3742G	possibly damaging	Het
Ficd	T	C	5: 113,738,690 (GRCm38)	F309L	probably benign	Het
Gfra1	A	T	19: 58,238,446 (GRCm38)	M451K	probably benign	Het
Gm43517	T	A	12: 49,391,178 (GRCm38)	L318Q		Het
Gsc	A	T	12: 104,473,051 (GRCm38)	V22E	possibly damaging	Het
Il4ra	T	C	7: 125,569,108 (GRCm38)	Y100H	probably damaging	Het
Ilf3	T	C	9: 21,398,044 (GRCm38)	S486P	probably benign	Het
Itga10	T	A	3: 96,652,953 (GRCm38)	H594Q	probably benign	Het
Kcnf1	T	C	12: 17,176,136 (GRCm38)	D28G	probably benign	Het
Kiss1	A	G	1: 133,329,422 (GRCm38)	K53R	possibly damaging	Het
Lcn5	A	G	2: 25,661,414 (GRCm38)	I182V	probably benign	Het
Lcorl	T	C	5: 45,701,965 (GRCm38)	D269G	probably benign	Het
Letm2	A	T	8: 25,592,537 (GRCm38)	C174*	probably null	Het
Met	T	G	6: 17,558,835 (GRCm38)	V1154G	probably damaging	Het
Mindy3	A	G	2: 12,419,189 (GRCm38)	S22P	probably benign	Het
Nfe2l3	T	C	6: 51,458,272 (GRCm38)	L604P	probably damaging	Het
Or12e10	A	G	2: 87,810,339 (GRCm38)	Q173R	probably damaging	Het
Or14a256	A	G	7: 86,616,561 (GRCm38)	F28S	possibly damaging	Het
Or5h18	T	A	16: 59,027,019 (GRCm38)	Q296L	possibly damaging	Het
Or8b3b	C	T	9: 38,672,623 (GRCm38)	V274I	probably benign	Het
Or8u9	A	T	2: 86,171,220 (GRCm38)	I199N	possibly damaging	Het
Or9r7	A	C	10: 130,126,586 (GRCm38)	I157S	probably damaging	Het
Pcdha9	T	A	18: 36,999,796 (GRCm38)	Y639*	probably null	Het
Pom121l2	A	T	13: 21,983,529 (GRCm38)	I657L	probably damaging	Het
Prr14l	T	A	5: 32,829,623 (GRCm38)	T843S	possibly damaging	Het
Rln1	A	T	19: 29,332,099 (GRCm38)	N93K	probably damaging	Het
Rpl3l	A	T	17: 24,732,427 (GRCm38)	M101L	probably benign	Het
Rtkn	A	G	6: 83,152,177 (GRCm38)	Y551C	probably benign	Het
Rundc1	G	C	11: 101,433,479 (GRCm38)	R337P	possibly damaging	Het
Scarf1	T	A	11: 75,514,416 (GRCm38)		probably null	Het
Serpinb1b	T	A	13: 33,091,639 (GRCm38)		probably null	Het
Sgsm1	A	T	5: 113,274,335 (GRCm38)	C484*	probably null	Het
Slc22a19	T	A	19: 7,673,303 (GRCm38)	K547*	probably null	Het
Slc22a19	T	C	19: 7,693,818 (GRCm38)	T177A	probably benign	Het
Slc6a5	A	C	7: 49,941,866 (GRCm38)	K526N	probably benign	Het
Sqle	G	A	15: 59,330,754 (GRCm38)	R519Q	probably benign	Het
Stard9	T	C	2: 120,688,146 (GRCm38)	I517T	probably benign	Het
Taf15	A	T	11: 83,505,023 (GRCm38)	D518V	unknown	Het
Tenm4	A	G	7: 96,895,985 (GRCm38)	M2440V	possibly damaging	Het
Tm6sf1	A	G	7: 81,868,710 (GRCm38)	M122V	possibly damaging	Het
Tmprss9	T	A	10: 80,892,219 (GRCm38)	S548T	possibly damaging	Het
Trim62	A	T	4: 128,883,678 (GRCm38)		probably benign	Het
Ugt2b34	C	T	5: 86,891,282 (GRCm38)	V507M	possibly damaging	Het
Usp16	T	A	16: 87,476,805 (GRCm38)	M423K	probably benign	Het
Vmn1r199	T	A	13: 22,382,736 (GRCm38)	S67T	probably benign	Het
Vmn1r62	A	T	7: 5,675,601 (GRCm38)	M94L	probably benign	Het
Wnk4	G	T	11: 101,264,354 (GRCm38)	E364*	probably null	Het
Xpo1	T	A	11: 23,282,584 (GRCm38)	I368N	probably damaging	Het
Zfp366	A	G	13: 99,246,296 (GRCm38)	T656A	probably benign	Het
Zfp934	T	C	13: 62,518,524 (GRCm38)	M113V	probably benign	Het
Zkscan2	A	G	7: 123,498,771 (GRCm38)	V134A	probably damaging	Het

Other mutations in Mroh2b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Mroh2b	APN	15	4,899,197 (GRCm38)	missense	probably benign
IGL00507:Mroh2b	APN	15	4,962,127 (GRCm38)	missense	probably damaging	1.00
IGL00548:Mroh2b	APN	15	4,931,316 (GRCm38)	missense	probably benign	0.35
IGL00902:Mroh2b	APN	15	4,915,222 (GRCm38)	missense	probably damaging	1.00
IGL00944:Mroh2b	APN	15	4,951,127 (GRCm38)	splice site	probably benign
IGL00954:Mroh2b	APN	15	4,903,054 (GRCm38)	missense	probably damaging	0.99
IGL01015:Mroh2b	APN	15	4,941,542 (GRCm38)	missense	probably damaging	1.00
IGL01134:Mroh2b	APN	15	4,915,152 (GRCm38)	missense	probably benign	0.00
IGL01337:Mroh2b	APN	15	4,905,024 (GRCm38)	missense	probably benign	0.38
IGL01780:Mroh2b	APN	15	4,912,000 (GRCm38)	missense	probably benign	0.01
IGL01919:Mroh2b	APN	15	4,923,688 (GRCm38)	missense	probably benign	0.10
IGL02069:Mroh2b	APN	15	4,904,324 (GRCm38)	splice site	probably benign
IGL02146:Mroh2b	APN	15	4,951,294 (GRCm38)	splice site	probably null
IGL02221:Mroh2b	APN	15	4,923,641 (GRCm38)	missense	probably damaging	1.00
IGL02281:Mroh2b	APN	15	4,952,263 (GRCm38)	missense	probably benign	0.04
IGL02350:Mroh2b	APN	15	4,912,000 (GRCm38)	missense	probably benign	0.01
IGL02357:Mroh2b	APN	15	4,912,000 (GRCm38)	missense	probably benign	0.01
IGL02401:Mroh2b	APN	15	4,900,501 (GRCm38)	missense	possibly damaging	0.71
IGL02427:Mroh2b	APN	15	4,951,560 (GRCm38)	splice site	probably benign
IGL02432:Mroh2b	APN	15	4,914,186 (GRCm38)	missense	probably benign
IGL02582:Mroh2b	APN	15	4,908,515 (GRCm38)	missense	probably damaging	0.98
IGL02632:Mroh2b	APN	15	4,931,101 (GRCm38)	missense	probably damaging	0.99
IGL02741:Mroh2b	APN	15	4,905,632 (GRCm38)	missense	probably benign
IGL02811:Mroh2b	APN	15	4,915,236 (GRCm38)	missense	possibly damaging	0.55
IGL02826:Mroh2b	APN	15	4,962,148 (GRCm38)	missense	probably damaging	0.99
IGL03412:Mroh2b	APN	15	4,944,372 (GRCm38)	missense	probably benign	0.14
PIT4468001:Mroh2b	UTSW	15	4,912,812 (GRCm38)	missense	probably damaging	1.00
R0024:Mroh2b	UTSW	15	4,925,627 (GRCm38)	missense	probably damaging	1.00
R0333:Mroh2b	UTSW	15	4,931,118 (GRCm38)	missense	probably damaging	1.00
R0433:Mroh2b	UTSW	15	4,941,634 (GRCm38)	missense	probably benign	0.01
R0530:Mroh2b	UTSW	15	4,934,395 (GRCm38)	missense	probably damaging	0.97
R1411:Mroh2b	UTSW	15	4,918,317 (GRCm38)	missense	probably damaging	1.00
R1457:Mroh2b	UTSW	15	4,925,684 (GRCm38)	missense	probably damaging	1.00
R1466:Mroh2b	UTSW	15	4,925,684 (GRCm38)	missense	probably damaging	1.00
R1466:Mroh2b	UTSW	15	4,925,684 (GRCm38)	missense	probably damaging	1.00
R1472:Mroh2b	UTSW	15	4,948,655 (GRCm38)	missense	probably benign	0.00
R1525:Mroh2b	UTSW	15	4,951,130 (GRCm38)	splice site	probably null
R1584:Mroh2b	UTSW	15	4,925,684 (GRCm38)	missense	probably damaging	1.00
R1605:Mroh2b	UTSW	15	4,945,090 (GRCm38)	missense	probably benign	0.08
R1657:Mroh2b	UTSW	15	4,931,043 (GRCm38)	nonsense	probably null
R1671:Mroh2b	UTSW	15	4,951,294 (GRCm38)	splice site	probably null
R1698:Mroh2b	UTSW	15	4,914,140 (GRCm38)	missense	probably benign	0.02
R2002:Mroh2b	UTSW	15	4,925,684 (GRCm38)	missense	probably damaging	1.00
R2005:Mroh2b	UTSW	15	4,917,158 (GRCm38)	missense	probably damaging	1.00
R2077:Mroh2b	UTSW	15	4,944,966 (GRCm38)	missense	probably damaging	1.00
R2179:Mroh2b	UTSW	15	4,921,446 (GRCm38)	critical splice donor site	probably null
R2183:Mroh2b	UTSW	15	4,918,225 (GRCm38)	splice site	probably null
R3713:Mroh2b	UTSW	15	4,943,649 (GRCm38)	missense	probably benign	0.01
R3714:Mroh2b	UTSW	15	4,943,649 (GRCm38)	missense	probably benign	0.01
R3747:Mroh2b	UTSW	15	4,952,246 (GRCm38)	nonsense	probably null
R3748:Mroh2b	UTSW	15	4,952,246 (GRCm38)	nonsense	probably null
R3749:Mroh2b	UTSW	15	4,952,246 (GRCm38)	nonsense	probably null
R3750:Mroh2b	UTSW	15	4,952,246 (GRCm38)	nonsense	probably null
R3792:Mroh2b	UTSW	15	4,923,620 (GRCm38)	missense	probably damaging	1.00
R3872:Mroh2b	UTSW	15	4,925,061 (GRCm38)	nonsense	probably null
R4021:Mroh2b	UTSW	15	4,925,100 (GRCm38)	missense	possibly damaging	0.75
R4329:Mroh2b	UTSW	15	4,931,379 (GRCm38)	missense	probably damaging	0.99
R4456:Mroh2b	UTSW	15	4,947,925 (GRCm38)	missense	probably benign	0.21
R4592:Mroh2b	UTSW	15	4,918,290 (GRCm38)	missense	probably damaging	1.00
R4836:Mroh2b	UTSW	15	4,904,270 (GRCm38)	missense	probably damaging	1.00
R5050:Mroh2b	UTSW	15	4,900,450 (GRCm38)	missense	possibly damaging	0.82
R5230:Mroh2b	UTSW	15	4,941,522 (GRCm38)	missense	probably benign	0.07
R5342:Mroh2b	UTSW	15	4,914,133 (GRCm38)	nonsense	probably null
R5353:Mroh2b	UTSW	15	4,917,178 (GRCm38)	missense	probably damaging	1.00
R5368:Mroh2b	UTSW	15	4,905,572 (GRCm38)	missense	probably damaging	1.00
R5424:Mroh2b	UTSW	15	4,941,612 (GRCm38)	missense	probably damaging	0.98
R5484:Mroh2b	UTSW	15	4,908,981 (GRCm38)	missense	possibly damaging	0.92
R5999:Mroh2b	UTSW	15	4,912,884 (GRCm38)	splice site	probably null
R6046:Mroh2b	UTSW	15	4,951,281 (GRCm38)	missense	probably benign	0.01
R6081:Mroh2b	UTSW	15	4,944,377 (GRCm38)	missense	probably damaging	1.00
R6162:Mroh2b	UTSW	15	4,915,225 (GRCm38)	missense	probably damaging	1.00
R6165:Mroh2b	UTSW	15	4,918,350 (GRCm38)	missense	probably benign	0.23
R6240:Mroh2b	UTSW	15	4,934,644 (GRCm38)	missense	probably benign	0.38
R6487:Mroh2b	UTSW	15	4,947,239 (GRCm38)	missense	probably damaging	1.00
R6539:Mroh2b	UTSW	15	4,905,574 (GRCm38)	missense	probably damaging	1.00
R6616:Mroh2b	UTSW	15	4,953,282 (GRCm38)	missense	probably benign	0.36
R6663:Mroh2b	UTSW	15	4,947,935 (GRCm38)	missense	probably benign	0.21
R6820:Mroh2b	UTSW	15	4,953,274 (GRCm38)	missense	probably damaging	1.00
R6900:Mroh2b	UTSW	15	4,908,987 (GRCm38)	missense	probably benign	0.00
R6990:Mroh2b	UTSW	15	4,912,802 (GRCm38)	missense	possibly damaging	0.55
R7067:Mroh2b	UTSW	15	4,900,504 (GRCm38)	missense	probably benign	0.35
R7092:Mroh2b	UTSW	15	4,934,678 (GRCm38)	missense	possibly damaging	0.92
R7102:Mroh2b	UTSW	15	4,948,003 (GRCm38)	missense	probably benign	0.06
R7264:Mroh2b	UTSW	15	4,921,362 (GRCm38)	missense	possibly damaging	0.81
R7436:Mroh2b	UTSW	15	4,941,554 (GRCm38)	missense	probably benign	0.21
R7462:Mroh2b	UTSW	15	4,908,627 (GRCm38)	missense	probably damaging	1.00
R7529:Mroh2b	UTSW	15	4,949,009 (GRCm38)	missense	probably damaging	1.00
R7575:Mroh2b	UTSW	15	4,934,605 (GRCm38)	missense	probably damaging	1.00
R7579:Mroh2b	UTSW	15	4,931,061 (GRCm38)	missense	probably benign	0.09
R7605:Mroh2b	UTSW	15	4,945,023 (GRCm38)	missense	probably damaging	1.00
R7797:Mroh2b	UTSW	15	4,949,105 (GRCm38)	missense	probably benign	0.36
R7848:Mroh2b	UTSW	15	4,938,379 (GRCm38)	nonsense	probably null
R7952:Mroh2b	UTSW	15	4,951,211 (GRCm38)	missense	probably damaging	1.00
R7995:Mroh2b	UTSW	15	4,921,357 (GRCm38)	nonsense	probably null
R8088:Mroh2b	UTSW	15	4,900,503 (GRCm38)	missense	possibly damaging	0.57
R8207:Mroh2b	UTSW	15	4,938,410 (GRCm38)	missense	possibly damaging	0.95
R8242:Mroh2b	UTSW	15	4,909,040 (GRCm38)	missense	probably benign	0.04
R8248:Mroh2b	UTSW	15	4,931,104 (GRCm38)	missense	probably benign	0.40
R8258:Mroh2b	UTSW	15	4,911,909 (GRCm38)	missense	probably benign	0.01
R8259:Mroh2b	UTSW	15	4,911,909 (GRCm38)	missense	probably benign	0.01
R8304:Mroh2b	UTSW	15	4,925,637 (GRCm38)	missense	probably damaging	0.99
R8316:Mroh2b	UTSW	15	4,951,264 (GRCm38)	nonsense	probably null
R8345:Mroh2b	UTSW	15	4,944,326 (GRCm38)	missense	probably benign	0.09
R8507:Mroh2b	UTSW	15	4,949,090 (GRCm38)	missense	probably damaging	1.00
R8728:Mroh2b	UTSW	15	4,905,640 (GRCm38)	missense	probably damaging	1.00
R8747:Mroh2b	UTSW	15	4,935,300 (GRCm38)	missense	probably damaging	0.99
R8798:Mroh2b	UTSW	15	4,948,709 (GRCm38)	missense	probably damaging	1.00
R8814:Mroh2b	UTSW	15	4,941,625 (GRCm38)	missense	possibly damaging	0.61
R8856:Mroh2b	UTSW	15	4,931,028 (GRCm38)	nonsense	probably null
R8910:Mroh2b	UTSW	15	4,931,373 (GRCm38)	missense	probably benign	0.01
R8913:Mroh2b	UTSW	15	4,917,528 (GRCm38)	intron	probably benign
R8941:Mroh2b	UTSW	15	4,962,124 (GRCm38)	missense	possibly damaging	0.86
R9014:Mroh2b	UTSW	15	4,899,188 (GRCm38)	start codon destroyed	probably null	0.95
R9086:Mroh2b	UTSW	15	4,953,272 (GRCm38)	critical splice acceptor site	probably null
R9101:Mroh2b	UTSW	15	4,900,453 (GRCm38)	missense	probably benign	0.20
R9118:Mroh2b	UTSW	15	4,962,091 (GRCm38)	missense	possibly damaging	0.86
R9393:Mroh2b	UTSW	15	4,951,184 (GRCm38)	missense	probably benign
R9429:Mroh2b	UTSW	15	4,934,425 (GRCm38)	missense	probably damaging	1.00
R9431:Mroh2b	UTSW	15	4,934,470 (GRCm38)	missense	probably damaging	1.00
R9443:Mroh2b	UTSW	15	4,944,339 (GRCm38)	missense	probably damaging	1.00
R9447:Mroh2b	UTSW	15	4,931,341 (GRCm38)	missense	probably damaging	1.00
R9497:Mroh2b	UTSW	15	4,921,363 (GRCm38)	missense	probably damaging	0.98
R9588:Mroh2b	UTSW	15	4,948,648 (GRCm38)	missense	probably benign	0.00
R9631:Mroh2b	UTSW	15	4,917,074 (GRCm38)	missense	probably damaging	0.97
R9686:Mroh2b	UTSW	15	4,945,123 (GRCm38)	missense	probably benign	0.34
R9774:Mroh2b	UTSW	15	4,914,131 (GRCm38)	missense	probably benign	0.08
X0067:Mroh2b	UTSW	15	4,951,591 (GRCm38)	missense	possibly damaging	0.90
Z1177:Mroh2b	UTSW	15	4,905,005 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTCTTTGACACAACCTGAAAACTG -3'
(R):5'- CCCGGGGAAAATTCAGACTG -3'

Sequencing Primer
(F):5'- TACTGACACACAAAAGAGGAGATTC -3'
(R):5'- ATTCAGACTGGGGGCAGGTATG -3'

Posted On

2019-10-24