Mutagenetix > Incidental Mutation

Incidental Mutation 'R7626:Zfp655'

589441

Institutional Source

Beutler Lab

Gene Symbol

Zfp655

Ensembl Gene

ENSMUSG00000007812

Gene Name

zinc finger protein 655

Synonyms

2700038I16Rik, 9030409O18Rik

MMRRC Submission

045690-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.451) question?

Stock #

R7626 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

145168525-145184112 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 145173917 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 136 (L136P)

Ref Sequence

ENSEMBL: ENSMUSP00000143198 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000085661] [ENSMUST00000167316] [ENSMUST00000196069] [ENSMUST00000199003] [ENSMUST00000199322] [ENSMUST00000200039]

AlphaFold

Q9CZP3

Predicted Effect

silent
Transcript: ENSMUST00000085661

SMART Domains

Protein: ENSMUSP00000082803
Gene: ENSMUSG00000007812

Domain	Start	End	E-Value	Type
KRAB	77	138	2.14e-23	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000167316

SMART Domains

Protein: ENSMUSP00000128969
Gene: ENSMUSG00000007812

Domain	Start	End	E-Value	Type
ZnF_C2H2	243	265	5.29e-5	SMART
ZnF_C2H2	271	293	2.99e-4	SMART
ZnF_C2H2	299	328	1.38e2	SMART
ZnF_C2H2	334	356	6.78e-3	SMART
ZnF_C2H2	361	383	3.99e0	SMART
ZnF_C2H2	411	433	3.63e-3	SMART
ZnF_C2H2	439	461	1.01e-1	SMART
low complexity region	463	475	N/A	INTRINSIC
ZnF_C2H2	495	517	6.47e1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000196069

Predicted Effect

unknown
Transcript: ENSMUST00000199003
AA Change: L65P

SMART Domains

Protein: ENSMUSP00000142825
Gene: ENSMUSG00000007812
AA Change: L65P

Domain	Start	End	E-Value	Type
Blast:KRAB	26	67	2e-9	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000199322

Predicted Effect

probably damaging
Transcript: ENSMUST00000200039
AA Change: L136P

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000143198
Gene: ENSMUSG00000007812
AA Change: L136P

Domain	Start	End	E-Value	Type
KRAB	77	138	2.14e-23	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

100% (56/56)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a zinc finger protein. The zinc finger proteins are involved in DNA binding and protein-protein interactions. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acta2	G	A	19: 34,229,931 (GRCm39)	T8I	probably benign	Het
Alox5	T	C	6: 116,390,756 (GRCm39)	D465G	possibly damaging	Het
Arhgef16	T	A	4: 154,367,339 (GRCm39)	K355N	possibly damaging	Het
Camkk2	T	A	5: 122,902,363 (GRCm39)		probably benign	Het
Ccdc171	C	T	4: 83,499,012 (GRCm39)	Q237*	probably null	Het
Ces2b	T	C	8: 105,564,017 (GRCm39)	Y464H	possibly damaging	Het
Cntfr	A	G	4: 41,662,013 (GRCm39)	F243S	possibly damaging	Het
Crip3	A	T	17: 46,740,791 (GRCm39)	R65S	probably benign	Het
Dcn	A	G	10: 97,319,340 (GRCm39)	Y39C	possibly damaging	Het
Degs2	T	C	12: 108,658,332 (GRCm39)	S216G	possibly damaging	Het
Dmxl1	A	G	18: 50,035,861 (GRCm39)	I2295V	probably benign	Het
Dnah2	A	G	11: 69,389,511 (GRCm39)	S794P	probably damaging	Het
Eri1	A	C	8: 35,941,554 (GRCm39)	Y264*	probably null	Het
Etl4	T	C	2: 20,718,189 (GRCm39)	L179P	probably damaging	Het
F5	T	G	1: 164,014,481 (GRCm39)	M584R	possibly damaging	Het
Fam98c	G	T	7: 28,852,248 (GRCm39)	R104S	probably damaging	Het
Flad1	A	T	3: 89,310,718 (GRCm39)	I443N	probably benign	Het
Hbp1	T	C	12: 31,993,899 (GRCm39)	E43G	probably benign	Het
Hivep3	CGG	CG	4: 119,955,108 (GRCm39)	1141	probably null	Het
Kcnj3	T	A	2: 55,484,833 (GRCm39)	C310*	probably null	Het
Lct	T	C	1: 128,212,932 (GRCm39)	Y1907C	probably damaging	Het
Loxhd1	T	C	18: 77,518,882 (GRCm39)	V1896A	possibly damaging	Het
Lrfn4	C	A	19: 4,663,679 (GRCm39)	R285L	probably damaging	Het
Ltb4r2	T	A	14: 56,000,338 (GRCm39)	S320T	probably damaging	Het
Mmd	G	T	11: 90,148,378 (GRCm39)	R20L	probably damaging	Het
Mmp1a	TG	TGG	9: 7,465,083 (GRCm38)		probably null	Het
Nbas	T	C	12: 13,608,661 (GRCm39)	S2146P	probably benign	Het
Nop14	A	G	5: 34,809,135 (GRCm39)	F319L	probably damaging	Het
Numa1	C	A	7: 101,648,630 (GRCm39)	T787K	probably benign	Het
Or10q12	T	G	19: 13,745,709 (GRCm39)	M1R	probably null	Het
Or2t46	A	C	11: 58,471,999 (GRCm39)	T110P	probably damaging	Het
Or6c208	T	G	10: 129,223,726 (GRCm39)	C75G	possibly damaging	Het
Patj	T	C	4: 98,435,224 (GRCm39)	V2A	probably benign	Het
Pisd	T	C	5: 32,898,032 (GRCm39)	H52R	probably benign	Het
Plekhg6	A	T	6: 125,340,631 (GRCm39)	D576E	probably benign	Het
Plekhn1	T	A	4: 156,310,110 (GRCm39)	H68L	probably benign	Het
Ppp1r37	T	A	7: 19,295,778 (GRCm39)	I60F	probably damaging	Het
Ppp2r1b	C	T	9: 50,789,476 (GRCm39)	T513M	possibly damaging	Het
Pramel32	A	T	4: 88,548,279 (GRCm39)	I42N	probably damaging	Het
Rhobtb2	C	A	14: 70,034,386 (GRCm39)	A280S	probably damaging	Het
Rnf40	A	G	7: 127,189,047 (GRCm39)	D140G	probably benign	Het
Rxfp1	A	G	3: 79,555,397 (GRCm39)	L653P	probably damaging	Het
Satb1	A	T	17: 52,074,995 (GRCm39)	D500E	probably benign	Het
Slc15a1	A	T	14: 121,713,377 (GRCm39)	D383E	probably benign	Het
Smarcad1	C	A	6: 65,073,033 (GRCm39)	S604R	possibly damaging	Het
St7	A	G	6: 17,934,216 (GRCm39)	T533A	probably damaging	Het
Sv2c	T	A	13: 96,122,451 (GRCm39)	T442S	probably benign	Het
Tbx2	A	G	11: 85,731,622 (GRCm39)	T640A	probably benign	Het
Tenm4	T	A	7: 96,542,221 (GRCm39)	N1948K	probably damaging	Het
Tll1	T	C	8: 64,551,268 (GRCm39)		probably null	Het
Tmem135	A	T	7: 88,805,718 (GRCm39)		probably null	Het
Tonsl	A	T	15: 76,518,136 (GRCm39)	L582Q	probably null	Het
Unc13c	T	A	9: 73,641,799 (GRCm39)	K1231N	probably damaging	Het
Vmn2r101	T	A	17: 19,832,192 (GRCm39)	Y729*	probably null	Het
Zcchc2	A	G	1: 105,928,742 (GRCm39)	T334A	possibly damaging	Het
Zfp729a	A	T	13: 67,768,437 (GRCm39)	C597*	probably null	Het

Other mutations in Zfp655

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00639:Zfp655	APN	5	145,180,955 (GRCm39)	missense	probably damaging	1.00
IGL01679:Zfp655	APN	5	145,180,637 (GRCm39)	missense	probably damaging	0.96
IGL02379:Zfp655	APN	5	145,180,765 (GRCm39)	missense	probably benign	0.13
IGL02647:Zfp655	APN	5	145,179,816 (GRCm39)	missense	probably benign
R0104:Zfp655	UTSW	5	145,180,825 (GRCm39)	missense	probably damaging	1.00
R0104:Zfp655	UTSW	5	145,180,825 (GRCm39)	missense	probably damaging	1.00
R0270:Zfp655	UTSW	5	145,181,267 (GRCm39)	missense	probably damaging	1.00
R0608:Zfp655	UTSW	5	145,180,867 (GRCm39)	missense	possibly damaging	0.57
R1528:Zfp655	UTSW	5	145,181,411 (GRCm39)	missense	probably damaging	0.98
R2076:Zfp655	UTSW	5	145,181,410 (GRCm39)	missense	possibly damaging	0.89
R2119:Zfp655	UTSW	5	145,181,594 (GRCm39)	missense	probably damaging	0.98
R2375:Zfp655	UTSW	5	145,181,206 (GRCm39)	missense	probably benign	0.10
R2403:Zfp655	UTSW	5	145,181,356 (GRCm39)	missense	probably benign	0.00
R4032:Zfp655	UTSW	5	145,180,858 (GRCm39)	missense	possibly damaging	0.50
R4532:Zfp655	UTSW	5	145,181,507 (GRCm39)	missense	probably benign	0.06
R4880:Zfp655	UTSW	5	145,181,168 (GRCm39)	missense	probably damaging	0.99
R5484:Zfp655	UTSW	5	145,180,445 (GRCm39)	missense	probably benign	0.01
R5529:Zfp655	UTSW	5	145,181,546 (GRCm39)	missense	probably damaging	0.96
R6193:Zfp655	UTSW	5	145,181,586 (GRCm39)	missense	probably benign	0.03
R6195:Zfp655	UTSW	5	145,180,572 (GRCm39)	missense	possibly damaging	0.52
R7050:Zfp655	UTSW	5	145,181,545 (GRCm39)	missense	probably benign	0.12
R7471:Zfp655	UTSW	5	145,181,542 (GRCm39)	missense	possibly damaging	0.80
R7612:Zfp655	UTSW	5	145,173,999 (GRCm39)	missense	unknown
R7989:Zfp655	UTSW	5	145,181,380 (GRCm39)	missense	probably damaging	1.00
R8557:Zfp655	UTSW	5	145,180,835 (GRCm39)	missense	probably benign	0.37
R8805:Zfp655	UTSW	5	145,181,290 (GRCm39)	missense	probably damaging	1.00
R9602:Zfp655	UTSW	5	145,181,473 (GRCm39)	missense	probably benign	0.00
R9664:Zfp655	UTSW	5	145,180,442 (GRCm39)	missense	probably damaging	1.00
Z1176:Zfp655	UTSW	5	145,180,813 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGCAGTCTCCTTGAGCTTTGTC -3'
(R):5'- ATGGTGGCATCCCAAGGAAC -3'

Sequencing Primer
(F):5'- AGCCTGTCTTTGATCTTGCATACAC -3'
(R):5'- GTGGCATCCCAAGGAACATATGTTC -3'

Posted On

2019-10-24