Mutagenetix > Incidental Mutation

Incidental Mutation 'R7629:Zfp362'

589538

Institutional Source

Beutler Lab

Gene Symbol

Zfp362

Ensembl Gene

ENSMUSG00000028799

Gene Name

zinc finger protein 362

Synonyms

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.324) question?

Stock #

R7629 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

128666881-128699838 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 128679848 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Serine at position 273 (R273S)

Ref Sequence

ENSEMBL: ENSMUSP00000065733 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071108] [ENSMUST00000106072] [ENSMUST00000170934]

AlphaFold

B1ASA5

Predicted Effect

probably damaging
Transcript: ENSMUST00000071108
AA Change: R273S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000065733
Gene: ENSMUSG00000028799
AA Change: R273S

Domain	Start	End	E-Value	Type
low complexity region	1	14	N/A	INTRINSIC
low complexity region	128	150	N/A	INTRINSIC
low complexity region	200	211	N/A	INTRINSIC
ZnF_C2H2	225	247	2.75e-3	SMART
ZnF_C2H2	253	275	1.98e-4	SMART
ZnF_C2H2	281	303	1.38e-3	SMART
ZnF_C2H2	309	333	3.49e-5	SMART
ZnF_C2H2	339	361	1.08e-1	SMART
ZnF_C2H2	369	391	7.26e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000106072
AA Change: R273S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000101686
Gene: ENSMUSG00000028799
AA Change: R273S

Domain	Start	End	E-Value	Type
low complexity region	1	14	N/A	INTRINSIC
low complexity region	128	150	N/A	INTRINSIC
low complexity region	200	211	N/A	INTRINSIC
ZnF_C2H2	225	247	2.75e-3	SMART
ZnF_C2H2	253	275	1.98e-4	SMART
ZnF_C2H2	281	303	1.38e-3	SMART
ZnF_C2H2	309	333	3.49e-5	SMART
ZnF_C2H2	339	361	1.08e-1	SMART
ZnF_C2H2	369	391	7.26e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000170934
AA Change: R260S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000128499
Gene: ENSMUSG00000028799
AA Change: R260S

Domain	Start	End	E-Value	Type
low complexity region	115	137	N/A	INTRINSIC
low complexity region	187	198	N/A	INTRINSIC
ZnF_C2H2	212	234	2.75e-3	SMART
ZnF_C2H2	240	262	1.98e-4	SMART
ZnF_C2H2	268	290	1.38e-3	SMART
ZnF_C2H2	296	320	3.49e-5	SMART
ZnF_C2H2	326	348	1.08e-1	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310061N02Rik	G	A	16: 88,504,293 (GRCm39)	T168I	probably benign	Het
Abtb2	T	A	2: 103,513,838 (GRCm39)		probably null	Het
Ankrd10	A	T	8: 11,665,769 (GRCm39)	V277E	probably benign	Het
Ankrd28	C	T	14: 31,437,221 (GRCm39)	V615I	probably benign	Het
Aqr	G	A	2: 113,945,074 (GRCm39)	P1079L	probably damaging	Het
Brox	G	A	1: 183,074,068 (GRCm39)	A60V	probably damaging	Het
Copg1	T	C	6: 87,871,151 (GRCm39)	V289A	possibly damaging	Het
Cplane1	C	T	15: 8,256,551 (GRCm39)	Q2004*	probably null	Het
Dido1	T	C	2: 180,303,266 (GRCm39)	N1546S	probably benign	Het
Dlg5	G	A	14: 24,295,280 (GRCm39)	P80L	probably damaging	Het
Dmxl1	T	A	18: 49,992,337 (GRCm39)	I361N	probably damaging	Het
Fan1	T	A	7: 64,022,234 (GRCm39)	N340Y	probably damaging	Het
Fhod3	T	A	18: 24,887,374 (GRCm39)	D88E	probably benign	Het
Flywch1	A	G	17: 23,974,744 (GRCm39)	M632T	probably benign	Het
Henmt1	A	G	3: 108,865,913 (GRCm39)	T213A	probably benign	Het
Itga9	T	C	9: 118,527,514 (GRCm39)	V555A	probably benign	Het
Katnip	T	C	7: 125,394,422 (GRCm39)	L192P	probably damaging	Het
Kif13b	C	T	14: 65,016,784 (GRCm39)	R1317*	probably null	Het
Kndc1	A	T	7: 139,475,176 (GRCm39)	E25V	probably damaging	Het
Lingo2	T	A	4: 35,708,675 (GRCm39)	D435V	possibly damaging	Het
Lrrc14b	T	A	13: 74,509,283 (GRCm39)	M375L	probably benign	Het
Lrrtm2	T	C	18: 35,347,310 (GRCm39)		probably null	Het
Mgat4c	G	A	10: 102,224,931 (GRCm39)	V382I	probably benign	Het
Muc16	T	C	9: 18,478,081 (GRCm39)	T7075A	possibly damaging	Het
Myo5b	T	A	18: 74,760,325 (GRCm39)		probably null	Het
Neb	T	C	2: 52,163,973 (GRCm39)	D1995G	possibly damaging	Het
Nudt9	T	C	5: 104,198,560 (GRCm39)	V75A	possibly damaging	Het
Or4k35	C	T	2: 111,100,221 (GRCm39)	V164I	probably benign	Het
Paf1	T	C	7: 28,094,493 (GRCm39)	Y35H	probably damaging	Het
Pals2	T	C	6: 50,173,603 (GRCm39)	I489T	probably benign	Het
Panx3	T	A	9: 37,572,740 (GRCm39)	Q270L	possibly damaging	Het
Pde6h	G	T	6: 136,936,317 (GRCm39)	R20L	possibly damaging	Het
Pdxk	A	G	10: 78,280,840 (GRCm39)	I200T	probably benign	Het
Ppara	A	T	15: 85,682,392 (GRCm39)	M363L	probably damaging	Het
Prmt8	A	T	6: 127,666,846 (GRCm39)	L376*	probably null	Het
Serpina1a	G	C	12: 103,820,067 (GRCm39)	T393R	probably damaging	Het
Sfxn1	C	T	13: 54,247,041 (GRCm39)	R178C	probably damaging	Het
Sirpb1c	A	T	3: 15,902,559 (GRCm39)	W7R	possibly damaging	Het
Skint5	T	C	4: 113,799,857 (GRCm39)	Y104C	probably damaging	Het
Slamf6	A	T	1: 171,764,191 (GRCm39)	T195S	probably damaging	Het
Slc2a6	T	C	2: 26,914,214 (GRCm39)	D301G	probably benign	Het
Stab2	A	T	10: 86,719,646 (GRCm39)		probably null	Het
Tctn3	C	G	19: 40,599,780 (GRCm39)	D141H	probably damaging	Het
Tnik	A	G	3: 28,715,877 (GRCm39)	N1164D	probably damaging	Het
Tpcn1	C	T	5: 120,676,002 (GRCm39)	V711I	probably benign	Het
Trim11	G	A	11: 58,869,160 (GRCm39)	G32D	probably damaging	Het
Usp32	TTTGGTTG	TTTG	11: 84,910,681 (GRCm39)		probably null	Het
Vmn1r72	T	C	7: 11,403,711 (GRCm39)	I246V	probably benign	Het
Zfp715	T	C	7: 42,951,100 (GRCm39)	D66G	possibly damaging	Het
Zftraf1	C	T	15: 76,532,386 (GRCm39)	D241N	probably benign	Het

Other mutations in Zfp362

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01667:Zfp362	APN	4	128,680,902 (GRCm39)	missense	probably damaging	1.00
IGL01939:Zfp362	APN	4	128,680,846 (GRCm39)	missense	probably benign
trek	UTSW	4	128,679,769 (GRCm39)	missense	probably damaging	1.00
Zax	UTSW	4	128,684,053 (GRCm39)	missense	possibly damaging	0.71
Zebulon	UTSW	4	128,679,808 (GRCm39)	missense	probably damaging	1.00
R1471:Zfp362	UTSW	4	128,680,993 (GRCm39)	missense	probably benign
R1892:Zfp362	UTSW	4	128,684,057 (GRCm39)	missense	probably benign	0.17
R1901:Zfp362	UTSW	4	128,684,069 (GRCm39)	missense	probably damaging	1.00
R2058:Zfp362	UTSW	4	128,680,780 (GRCm39)	missense	possibly damaging	0.71
R3739:Zfp362	UTSW	4	128,680,682 (GRCm39)	splice site	probably benign
R6772:Zfp362	UTSW	4	128,684,053 (GRCm39)	missense	possibly damaging	0.71
R6900:Zfp362	UTSW	4	128,679,808 (GRCm39)	missense	probably damaging	1.00
R6901:Zfp362	UTSW	4	128,679,808 (GRCm39)	missense	probably damaging	1.00
R7105:Zfp362	UTSW	4	128,668,319 (GRCm39)	missense	probably damaging	0.99
R7348:Zfp362	UTSW	4	128,671,010 (GRCm39)	missense	possibly damaging	0.88
R7712:Zfp362	UTSW	4	128,671,203 (GRCm39)	missense	probably benign	0.14
R7763:Zfp362	UTSW	4	128,680,824 (GRCm39)	missense	probably benign
R8434:Zfp362	UTSW	4	128,679,769 (GRCm39)	missense	probably damaging	1.00
R8508:Zfp362	UTSW	4	128,668,399 (GRCm39)	missense	probably damaging	1.00
R9432:Zfp362	UTSW	4	128,670,980 (GRCm39)	missense	probably damaging	1.00
R9567:Zfp362	UTSW	4	128,688,681 (GRCm39)	start codon destroyed	unknown
Z1177:Zfp362	UTSW	4	128,668,343 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CCTAAAAGGCCCATCAGAGG -3'
(R):5'- ATCTTCCCAGTCTTGAAGCCG -3'

Sequencing Primer
(F):5'- GTGACAGACATCCGAGAGTCC -3'
(R):5'- CGCCTCTTGCCTTATAGGTGTAAG -3'

Posted On

2019-10-24