Mutagenetix > Incidental Mutations

Incidental Mutation 'R7629:Zfp362'

589538

Institutional Source

Beutler Lab

Gene Symbol

Zfp362

Ensembl Gene

ENSMUSG00000028799

Gene Name

zinc finger protein 362

Synonyms

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.202) question?

Stock #

R7629 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

128773088-128806045 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 128786055 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Serine at position 273 (R273S)

Ref Sequence

ENSEMBL: ENSMUSP00000065733 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071108] [ENSMUST00000106072] [ENSMUST00000170934]

AlphaFold

B1ASA5

Predicted Effect

probably damaging
Transcript: ENSMUST00000071108
AA Change: R273S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000065733
Gene: ENSMUSG00000028799
AA Change: R273S

Domain	Start	End	E-Value	Type
low complexity region	1	14	N/A	INTRINSIC
low complexity region	128	150	N/A	INTRINSIC
low complexity region	200	211	N/A	INTRINSIC
ZnF_C2H2	225	247	2.75e-3	SMART
ZnF_C2H2	253	275	1.98e-4	SMART
ZnF_C2H2	281	303	1.38e-3	SMART
ZnF_C2H2	309	333	3.49e-5	SMART
ZnF_C2H2	339	361	1.08e-1	SMART
ZnF_C2H2	369	391	7.26e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000106072
AA Change: R273S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000101686
Gene: ENSMUSG00000028799
AA Change: R273S

Domain	Start	End	E-Value	Type
low complexity region	1	14	N/A	INTRINSIC
low complexity region	128	150	N/A	INTRINSIC
low complexity region	200	211	N/A	INTRINSIC
ZnF_C2H2	225	247	2.75e-3	SMART
ZnF_C2H2	253	275	1.98e-4	SMART
ZnF_C2H2	281	303	1.38e-3	SMART
ZnF_C2H2	309	333	3.49e-5	SMART
ZnF_C2H2	339	361	1.08e-1	SMART
ZnF_C2H2	369	391	7.26e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000170934
AA Change: R260S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000128499
Gene: ENSMUSG00000028799
AA Change: R260S

Domain	Start	End	E-Value	Type
low complexity region	115	137	N/A	INTRINSIC
low complexity region	187	198	N/A	INTRINSIC
ZnF_C2H2	212	234	2.75e-3	SMART
ZnF_C2H2	240	262	1.98e-4	SMART
ZnF_C2H2	268	290	1.38e-3	SMART
ZnF_C2H2	296	320	3.49e-5	SMART
ZnF_C2H2	326	348	1.08e-1	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310061N02Rik	G	A	16: 88,707,405	T168I	probably benign	Het
2410089E03Rik	C	T	15: 8,227,067	Q2004*	probably null	Het
Abtb2	T	A	2: 103,683,493		probably null	Het
Ankrd10	A	T	8: 11,615,769	V277E	probably benign	Het
Ankrd28	C	T	14: 31,715,264	V615I	probably benign	Het
Aqr	G	A	2: 114,114,593	P1079L	probably damaging	Het
Brox	G	A	1: 183,292,504	A60V	probably damaging	Het
Copg1	T	C	6: 87,894,169	V289A	possibly damaging	Het
Cyhr1	C	T	15: 76,648,186	D241N	probably benign	Het
D430042O09Rik	T	C	7: 125,795,250	L192P	probably damaging	Het
Dido1	T	C	2: 180,661,473	N1546S	probably benign	Het
Dlg5	G	A	14: 24,245,212	P80L	probably damaging	Het
Dmxl1	T	A	18: 49,859,270	I361N	probably damaging	Het
Fan1	T	A	7: 64,372,486	N340Y	probably damaging	Het
Fhod3	T	A	18: 24,754,317	D88E	probably benign	Het
Flywch1	A	G	17: 23,755,770	M632T	probably benign	Het
Henmt1	A	G	3: 108,958,597	T213A	probably benign	Het
Itga9	T	C	9: 118,698,446	V555A	probably benign	Het
Kif13b	C	T	14: 64,779,335	R1317*	probably null	Het
Kndc1	A	T	7: 139,895,260	E25V	probably damaging	Het
Lingo2	T	A	4: 35,708,675	D435V	possibly damaging	Het
Lrrc14b	T	A	13: 74,361,164	M375L	probably benign	Het
Lrrtm2	T	C	18: 35,214,257		probably null	Het
Mgat4c	G	A	10: 102,389,070	V382I	probably benign	Het
Mpp6	T	C	6: 50,196,623	I489T	probably benign	Het
Muc16	T	C	9: 18,566,785	T7075A	possibly damaging	Het
Myo5b	T	A	18: 74,627,254		probably null	Het
Neb	T	C	2: 52,273,961	D1995G	possibly damaging	Het
Nudt9	T	C	5: 104,050,694	V75A	possibly damaging	Het
Olfr1277	C	T	2: 111,269,876	V164I	probably benign	Het
Paf1	T	C	7: 28,395,068	Y35H	probably damaging	Het
Panx3	T	A	9: 37,661,444	Q270L	possibly damaging	Het
Pde6h	G	T	6: 136,959,319	R20L	possibly damaging	Het
Pdxk	A	G	10: 78,445,006	I200T	probably benign	Het
Ppara	A	T	15: 85,798,191	M363L	probably damaging	Het
Prmt8	A	T	6: 127,689,883	L376*	probably null	Het
Serpina1a	G	C	12: 103,853,808	T393R	probably damaging	Het
Sfxn1	C	T	13: 54,093,022	R178C	probably damaging	Het
Sirpb1c	A	T	3: 15,848,395	W7R	possibly damaging	Het
Skint5	T	C	4: 113,942,660	Y104C	probably damaging	Het
Slamf6	A	T	1: 171,936,624	T195S	probably damaging	Het
Slc2a6	T	C	2: 27,024,202	D301G	probably benign	Het
Stab2	A	T	10: 86,883,782		probably null	Het
Tctn3	C	G	19: 40,611,336	D141H	probably damaging	Het
Tnik	A	G	3: 28,661,728	N1164D	probably damaging	Het
Tpcn1	C	T	5: 120,537,937	V711I	probably benign	Het
Trim11	G	A	11: 58,978,334	G32D	probably damaging	Het
Usp32	TTTGGTTG	TTTG	11: 85,019,855		probably null	Het
Vmn1r72	T	C	7: 11,669,784	I246V	probably benign	Het
Zfp715	T	C	7: 43,301,676	D66G	possibly damaging	Het

Other mutations in Zfp362

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01667:Zfp362	APN	4	128787109	missense	probably damaging	1.00
IGL01939:Zfp362	APN	4	128787053	missense	probably benign
trek	UTSW	4	128785976	missense	probably damaging	1.00
Zax	UTSW	4	128790260	missense	possibly damaging	0.71
Zebulon	UTSW	4	128786015	missense	probably damaging	1.00
R1471:Zfp362	UTSW	4	128787200	missense	probably benign
R1892:Zfp362	UTSW	4	128790264	missense	probably benign	0.17
R1901:Zfp362	UTSW	4	128790276	missense	probably damaging	1.00
R2058:Zfp362	UTSW	4	128786987	missense	possibly damaging	0.71
R3739:Zfp362	UTSW	4	128786889	splice site	probably benign
R6772:Zfp362	UTSW	4	128790260	missense	possibly damaging	0.71
R6900:Zfp362	UTSW	4	128786015	missense	probably damaging	1.00
R6901:Zfp362	UTSW	4	128786015	missense	probably damaging	1.00
R7105:Zfp362	UTSW	4	128774526	missense	probably damaging	0.99
R7348:Zfp362	UTSW	4	128777217	missense	possibly damaging	0.88
R7712:Zfp362	UTSW	4	128777410	missense	probably benign	0.14
R7763:Zfp362	UTSW	4	128787031	missense	probably benign
R8434:Zfp362	UTSW	4	128785976	missense	probably damaging	1.00
R8508:Zfp362	UTSW	4	128774606	missense	probably damaging	1.00
R9432:Zfp362	UTSW	4	128777187	missense	not run
Z1177:Zfp362	UTSW	4	128774550	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CCTAAAAGGCCCATCAGAGG -3'
(R):5'- ATCTTCCCAGTCTTGAAGCCG -3'

Sequencing Primer
(F):5'- GTGACAGACATCCGAGAGTCC -3'
(R):5'- CGCCTCTTGCCTTATAGGTGTAAG -3'

Posted On

2019-10-24