Mutagenetix > Incidental Mutation

Incidental Mutation 'R7629:Ankrd10'

589551

Institutional Source

Beutler Lab

Gene Symbol

Ankrd10

Ensembl Gene

ENSMUSG00000031508

Gene Name

ankyrin repeat domain 10

Synonyms

4833425P12Rik

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R7629 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

11611583-11635757 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 11615769 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 277 (V277E)

Ref Sequence

ENSEMBL: ENSMUSP00000131657 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033905] [ENSMUST00000169782] [ENSMUST00000209915] [ENSMUST00000210530] [ENSMUST00000211174]

AlphaFold

Q99LW0

Predicted Effect

probably benign
Transcript: ENSMUST00000033905

SMART Domains

Protein: ENSMUSP00000033905
Gene: ENSMUSG00000031508

Domain	Start	End	E-Value	Type
Blast:ANK	20	49	2e-10	BLAST
ANK	56	85	2.66e-5	SMART
ANK	90	119	6.46e-4	SMART
ANK	123	152	1.03e-2	SMART
Blast:ANK	156	185	6e-6	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000169782
AA Change: V277E

PolyPhen 2 Score 0.127 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000131657
Gene: ENSMUSG00000031508
AA Change: V277E

Domain	Start	End	E-Value	Type
Blast:ANK	20	49	1e-10	BLAST
ANK	56	85	2.66e-5	SMART
ANK	90	119	6.46e-4	SMART
ANK	123	152	1.03e-2	SMART
Blast:ANK	156	185	4e-6	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000209915

Predicted Effect

probably benign
Transcript: ENSMUST00000210530

Predicted Effect

probably benign
Transcript: ENSMUST00000211174

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310061N02Rik	G	A	16: 88,707,405	T168I	probably benign	Het
2410089E03Rik	C	T	15: 8,227,067	Q2004*	probably null	Het
Abtb2	T	A	2: 103,683,493		probably null	Het
Ankrd28	C	T	14: 31,715,264	V615I	probably benign	Het
Aqr	G	A	2: 114,114,593	P1079L	probably damaging	Het
Brox	G	A	1: 183,292,504	A60V	probably damaging	Het
Copg1	T	C	6: 87,894,169	V289A	possibly damaging	Het
Cyhr1	C	T	15: 76,648,186	D241N	probably benign	Het
D430042O09Rik	T	C	7: 125,795,250	L192P	probably damaging	Het
Dido1	T	C	2: 180,661,473	N1546S	probably benign	Het
Dlg5	G	A	14: 24,245,212	P80L	probably damaging	Het
Dmxl1	T	A	18: 49,859,270	I361N	probably damaging	Het
Fan1	T	A	7: 64,372,486	N340Y	probably damaging	Het
Fhod3	T	A	18: 24,754,317	D88E	probably benign	Het
Flywch1	A	G	17: 23,755,770	M632T	probably benign	Het
Henmt1	A	G	3: 108,958,597	T213A	probably benign	Het
Itga9	T	C	9: 118,698,446	V555A	probably benign	Het
Kif13b	C	T	14: 64,779,335	R1317*	probably null	Het
Kndc1	A	T	7: 139,895,260	E25V	probably damaging	Het
Lingo2	T	A	4: 35,708,675	D435V	possibly damaging	Het
Lrrc14b	T	A	13: 74,361,164	M375L	probably benign	Het
Lrrtm2	T	C	18: 35,214,257		probably null	Het
Mgat4c	G	A	10: 102,389,070	V382I	probably benign	Het
Mpp6	T	C	6: 50,196,623	I489T	probably benign	Het
Muc16	T	C	9: 18,566,785	T7075A	possibly damaging	Het
Myo5b	T	A	18: 74,627,254		probably null	Het
Neb	T	C	2: 52,273,961	D1995G	possibly damaging	Het
Nudt9	T	C	5: 104,050,694	V75A	possibly damaging	Het
Olfr1277	C	T	2: 111,269,876	V164I	probably benign	Het
Paf1	T	C	7: 28,395,068	Y35H	probably damaging	Het
Panx3	T	A	9: 37,661,444	Q270L	possibly damaging	Het
Pde6h	G	T	6: 136,959,319	R20L	possibly damaging	Het
Pdxk	A	G	10: 78,445,006	I200T	probably benign	Het
Ppara	A	T	15: 85,798,191	M363L	probably damaging	Het
Prmt8	A	T	6: 127,689,883	L376*	probably null	Het
Serpina1a	G	C	12: 103,853,808	T393R	probably damaging	Het
Sfxn1	C	T	13: 54,093,022	R178C	probably damaging	Het
Sirpb1c	A	T	3: 15,848,395	W7R	possibly damaging	Het
Skint5	T	C	4: 113,942,660	Y104C	probably damaging	Het
Slamf6	A	T	1: 171,936,624	T195S	probably damaging	Het
Slc2a6	T	C	2: 27,024,202	D301G	probably benign	Het
Stab2	A	T	10: 86,883,782		probably null	Het
Tctn3	C	G	19: 40,611,336	D141H	probably damaging	Het
Tnik	A	G	3: 28,661,728	N1164D	probably damaging	Het
Tpcn1	C	T	5: 120,537,937	V711I	probably benign	Het
Trim11	G	A	11: 58,978,334	G32D	probably damaging	Het
Usp32	TTTGGTTG	TTTG	11: 85,019,855		probably null	Het
Vmn1r72	T	C	7: 11,669,784	I246V	probably benign	Het
Zfp362	G	T	4: 128,786,055	R273S	probably damaging	Het
Zfp715	T	C	7: 43,301,676	D66G	possibly damaging	Het

Other mutations in Ankrd10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01480:Ankrd10	APN	8	11635592	missense	probably benign
IGL03029:Ankrd10	APN	8	11619304	splice site	probably null
R0098:Ankrd10	UTSW	8	11612560	missense	probably benign	0.01
R0393:Ankrd10	UTSW	8	11635482	missense	possibly damaging	0.58
R2870:Ankrd10	UTSW	8	11615682	missense	probably damaging	0.98
R2870:Ankrd10	UTSW	8	11615682	missense	probably damaging	0.98
R4176:Ankrd10	UTSW	8	11612644	missense	probably benign	0.00
R5985:Ankrd10	UTSW	8	11619077	nonsense	probably null
R6999:Ankrd10	UTSW	8	11619106	missense	probably damaging	1.00
R7108:Ankrd10	UTSW	8	11612624	missense	probably damaging	1.00
R7322:Ankrd10	UTSW	8	11615841	missense	probably damaging	0.97
R7522:Ankrd10	UTSW	8	11632910	missense	probably damaging	1.00
R7559:Ankrd10	UTSW	8	11612548	missense	probably damaging	0.97
R8263:Ankrd10	UTSW	8	11615707	missense	probably benign	0.22
R8493:Ankrd10	UTSW	8	11628518	nonsense	probably null
R8713:Ankrd10	UTSW	8	11628491	missense	probably damaging	1.00
R8897:Ankrd10	UTSW	8	11615788	missense	possibly damaging	0.61

Predicted Primers

PCR Primer
(F):5'- TCTTGCATCTGTACAAGTGCC -3'
(R):5'- GCGATGCAAAGCACTGTCTTTG -3'

Sequencing Primer
(F):5'- CACTGGACAAGAAAGTGC -3'
(R):5'- GCTTTCTTTATGGACACGGAATC -3'

Posted On

2019-10-24