Mutagenetix > Incidental Mutation

Incidental Mutation 'R7633:Gm21731'

589781

Institutional Source

Beutler Lab

Gene Symbol

Gm21731

Ensembl Gene

ENSMUSG00000063846

Gene Name

predicted gene, 21731

Synonyms

MMRRC Submission

045721-MU

Accession Numbers

Essential gene?

Not available question?

Stock #

R7633 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

120239111-120241367 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 120240840 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Stop codon at position 57 (W57*)

Ref Sequence

ENSEMBL: ENSMUSP00000137283 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079524] [ENSMUST00000223770]

AlphaFold

A0A286YDE9

Predicted Effect

probably null
Transcript: ENSMUST00000079524
AA Change: W57*

SMART Domains

Protein: ENSMUSP00000137283
Gene: ENSMUSG00000063846
AA Change: W57*

Domain	Start	End	E-Value	Type
Pfam:DUF1438	1	150	3.5e-90	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000223770
AA Change: W57*

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (52/52)

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930504O13Rik	C	A	11: 58,447,478	M29I	probably benign	Het
A530084C06Rik	G	T	13: 31,558,995	R92S	unknown	Het
Acaca	A	G	11: 84,372,639	I2029V	probably benign	Het
Anks1b	A	C	10: 90,948,584	D1138A	probably damaging	Het
Atr	T	A	9: 95,947,118	V2508E	probably damaging	Het
C4b	C	T	17: 34,729,399		probably null	Het
C530008M17Rik	A	G	5: 76,857,520	E576G	unknown	Het
Cd46	A	T	1: 195,083,619	M146K	probably null	Het
Chp1	A	G	2: 119,560,745	I28V	probably benign	Het
Chrnb1	T	A	11: 69,792,873	R216W	probably damaging	Het
Clmn	G	A	12: 104,782,112	T392M	probably benign	Het
Dcxr	A	C	11: 120,726,453	L88R	probably benign	Het
Dedd	C	T	1: 171,338,910	P138L	probably benign	Het
Degs1	A	G	1: 182,279,698	S35P	probably damaging	Het
Ehmt1	T	C	2: 24,815,780	D851G	possibly damaging	Het
Fam117b	A	G	1: 59,981,534	D521G	probably damaging	Het
Fam26d	G	A	10: 34,043,908	T121M	possibly damaging	Het
Gm14085	A	T	2: 122,486,680	R7S	probably null	Het
Gm6882	A	G	7: 21,427,652	V97A	probably damaging	Het
Hgs	A	T	11: 120,474,302	Q172L	probably damaging	Het
Hormad2	T	C	11: 4,346,662	Q274R	probably benign	Het
Igkv8-19	C	T	6: 70,341,399		probably benign	Het
Ikbkb	T	C	8: 22,671,741	N377S	probably benign	Het
Intu	G	A	3: 40,654,253	G232S	probably damaging	Het
Mov10	A	G	3: 104,797,065	S806P	possibly damaging	Het
Mrgprb1	A	G	7: 48,447,583	F194L	probably benign	Het
Notch3	A	T	17: 32,158,622	I160K	probably benign	Het
Nup155	C	G	15: 8,109,453	S3W	probably damaging	Het
Olfr1287	A	G	2: 111,449,622	I161V	probably benign	Het
Olfr284	A	G	15: 98,340,086	I301T	probably damaging	Het
Plxdc1	A	T	11: 97,956,151	F147L	possibly damaging	Het
Ppfia1	T	C	7: 144,552,436	D33G	possibly damaging	Het
Prdm11	G	A	2: 92,980,654	A200V	probably damaging	Het
Qrich2	A	T	11: 116,456,629	I1123K	unknown	Het
Rgs1	A	G	1: 144,248,477		probably null	Het
Skint7	T	A	4: 111,984,140	V259E	probably benign	Het
Slc25a3	A	T	10: 91,118,042		probably null	Het
Slco1a6	A	T	6: 142,145,755	I73N	probably damaging	Het
Sltm	C	G	9: 70,586,673	P802R	possibly damaging	Het
Smc4	CTA	CTATA	3: 69,018,067		probably benign	Het
Spock2	G	A	10: 60,126,180	D206N	probably damaging	Het
Stard3nl	T	G	13: 19,367,838	R185S	probably damaging	Het
Supt16	A	G	14: 52,197,099	Y18H	probably benign	Het
Synpo	G	A	18: 60,603,428	T482I	probably damaging	Het
Tmc6	G	T	11: 117,769,220	T694K	probably benign	Het
Trim59	T	A	3: 69,037,926	H27L	probably damaging	Het
Trpv2	G	A	11: 62,591,006		probably null	Het
Tyw5	A	T	1: 57,393,485	D117E	probably benign	Het
Usp17la	T	C	7: 104,861,147	Y320H	probably damaging	Het
Usp31	T	C	7: 121,658,962	D694G	probably damaging	Het
Vmn1r159	T	A	7: 22,843,023	T195S	probably damaging	Het
Vmn2r57	C	T	7: 41,425,089	V517M	possibly damaging	Het
Yipf1	A	G	4: 107,318,996	N29S	probably benign	Het
Zfp954	A	T	7: 7,115,824	F240L	possibly damaging	Het

Other mutations in Gm21731

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1600:Gm21731	UTSW	13	120,240,833 (GRCm38)	missense	probably benign	0.00
R3792:Gm21731	UTSW	13	120,240,931 (GRCm38)	missense	probably damaging	0.98
R4674:Gm21731	UTSW	13	120,240,826 (GRCm38)	missense	probably damaging	0.99
R4675:Gm21731	UTSW	13	120,240,826 (GRCm38)	missense	probably damaging	0.99
R4925:Gm21731	UTSW	13	120,240,848 (GRCm38)	missense	probably damaging	1.00
R6538:Gm21731	UTSW	13	120,240,932 (GRCm38)	missense	possibly damaging	0.72
R7186:Gm21731	UTSW	13	120,241,051 (GRCm38)	missense	probably damaging	0.97
R7541:Gm21731	UTSW	13	120,240,979 (GRCm38)	missense	probably benign
R8669:Gm21731	UTSW	13	120,240,802 (GRCm38)	missense	probably benign
R8997:Gm21731	UTSW	13	120,240,979 (GRCm38)	missense	probably benign
R9648:Gm21731	UTSW	13	120,240,959 (GRCm38)	missense	possibly damaging	0.90
Z1177:Gm21731	UTSW	13	120,241,172 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- AATGAAGCGGTTCTGTCCCTG -3'
(R):5'- ACCATCACAGGTCTCAAGTTCC -3'

Sequencing Primer
(F):5'- AAGCGGTTCTGTCCCTGTCTTG -3'
(R):5'- CCTGAGCAAGCTTGGCAAG -3'

Posted On

2019-10-24