Mutagenetix > Incidental Mutation

Incidental Mutation 'R7638:Megf11'

590074

Institutional Source

Beutler Lab

Gene Symbol

Megf11

Ensembl Gene

ENSMUSG00000036466

Gene Name

multiple EGF-like-domains 11

Synonyms

MMRRC Submission

045696-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.107) question?

Stock #

R7638 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

64385626-64709205 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 64679253 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 422 (N422K)

Ref Sequence

ENSEMBL: ENSMUSP00000091349 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068967] [ENSMUST00000093829] [ENSMUST00000118485] [ENSMUST00000164113]

AlphaFold

Q80T91

Predicted Effect

probably damaging
Transcript: ENSMUST00000068967
AA Change: N453K

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000065353
Gene: ENSMUSG00000036466
AA Change: N453K

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
low complexity region	74	84	N/A	INTRINSIC
EGF	101	129	1.85e0	SMART
EGF_Lam	145	184	9.55e-3	SMART
EGF_Lam	188	227	2e-5	SMART
EGF	226	258	5.57e-4	SMART
EGF_Lam	274	313	1.26e-2	SMART
EGF_Lam	317	357	2.52e-2	SMART
EGF_Lam	361	402	4.16e-3	SMART
EGF	401	433	6.21e-2	SMART
EGF	444	476	4.1e-2	SMART
EGF	487	519	7.02e-1	SMART
EGF_Lam	535	574	1.43e-1	SMART
EGF_Lam	578	617	5.04e-2	SMART
EGF	616	650	8.52e0	SMART
EGF	661	693	9.41e-2	SMART
EGF	704	736	8.52e0	SMART
EGF_Lam	752	791	2.99e-4	SMART
EGF	790	822	1.14e0	SMART
transmembrane domain	848	870	N/A	INTRINSIC
low complexity region	895	909	N/A	INTRINSIC
low complexity region	1022	1037	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000093829
AA Change: N422K

PolyPhen 2 Score 0.965 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000091349
Gene: ENSMUSG00000036466
AA Change: N422K

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
low complexity region	74	84	N/A	INTRINSIC
EGF_Lam	114	153	9.55e-3	SMART
EGF_Lam	157	196	2e-5	SMART
EGF	195	227	5.57e-4	SMART
EGF_Lam	243	282	1.26e-2	SMART
EGF_Lam	286	326	2.52e-2	SMART
EGF_Lam	330	371	4.16e-3	SMART
EGF	370	402	6.21e-2	SMART
EGF	413	445	4.1e-2	SMART
EGF	456	488	7.02e-1	SMART
EGF_Lam	504	543	1.43e-1	SMART
EGF_Lam	547	586	5.04e-2	SMART
EGF	585	619	8.52e0	SMART
EGF	630	662	9.41e-2	SMART
EGF_Lam	678	717	2.99e-4	SMART
EGF	716	748	1.14e0	SMART
transmembrane domain	774	796	N/A	INTRINSIC
low complexity region	821	835	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000118485
AA Change: N453K

PolyPhen 2 Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000114035
Gene: ENSMUSG00000036466
AA Change: N453K

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
low complexity region	74	84	N/A	INTRINSIC
EGF	101	129	1.85e0	SMART
EGF_Lam	145	184	9.55e-3	SMART
EGF_Lam	188	227	2e-5	SMART
EGF	226	258	5.57e-4	SMART
EGF_Lam	274	313	1.26e-2	SMART
EGF_Lam	317	357	2.52e-2	SMART
EGF_Lam	361	402	4.16e-3	SMART
EGF	401	433	6.21e-2	SMART
EGF	444	476	4.1e-2	SMART
EGF	487	519	7.02e-1	SMART
EGF_Lam	535	574	1.43e-1	SMART
EGF_Lam	578	617	5.04e-2	SMART
EGF	616	650	8.52e0	SMART
EGF	661	693	9.41e-2	SMART
EGF	704	736	8.52e0	SMART
EGF_Lam	752	791	2.99e-4	SMART
EGF	790	822	1.14e0	SMART
transmembrane domain	848	870	N/A	INTRINSIC
low complexity region	926	941	N/A	INTRINSIC

Predicted Effect

SMART Domains

Protein: ENSMUSP00000120514
Gene: ENSMUSG00000036466
AA Change: N43K

Domain	Start	End	E-Value	Type
EGF_like	3	36	2.79e0	SMART
EGF	35	67	4.1e-2	SMART
EGF	78	110	7.02e-1	SMART
EGF_Lam	126	165	1.43e-1	SMART
EGF	164	196	1.64e-1	SMART
EGF_Lam	212	251	2.99e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000164113
AA Change: N453K

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000128672
Gene: ENSMUSG00000036466
AA Change: N453K

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
low complexity region	74	84	N/A	INTRINSIC
EGF	101	129	1.85e0	SMART
EGF_Lam	145	184	9.55e-3	SMART
EGF_Lam	188	227	2e-5	SMART
EGF	226	258	5.57e-4	SMART
EGF_Lam	274	313	1.26e-2	SMART
EGF_Lam	317	357	2.52e-2	SMART
EGF_Lam	361	402	4.16e-3	SMART
EGF	401	433	6.21e-2	SMART
EGF	444	476	4.1e-2	SMART
EGF	487	519	7.02e-1	SMART
EGF_Lam	535	574	1.43e-1	SMART
EGF_Lam	578	617	5.04e-2	SMART
EGF	616	650	8.52e0	SMART
EGF	661	693	9.41e-2	SMART
EGF_Lam	709	748	2.99e-4	SMART
EGF	747	779	1.14e0	SMART
transmembrane domain	805	827	N/A	INTRINSIC
low complexity region	852	866	N/A	INTRINSIC
low complexity region	979	994	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (71/71)

MGI Phenotype

PHENOTYPE: Mice homozygous for a targeted allele exhibit abnormal spacing of horizontal cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930563D23Rik	A	G	16: 92,320,917	V161A	probably damaging	Het
Abo	T	G	2: 26,843,843	T115P	probably damaging	Het
Aggf1	G	A	13: 95,356,413	R563*	probably null	Het
Amdhd1	A	T	10: 93,534,498	Y159*	probably null	Het
BC027072	T	C	17: 71,750,885	D599G	probably damaging	Het
C77080	T	C	4: 129,221,941	T1022A	probably benign	Het
Camp	T	C	9: 109,848,393	E124G		Het
Casq2	A	G	3: 102,086,700	E21G	possibly damaging	Het
Cbln1	A	T	8: 87,471,729	F116Y	probably damaging	Het
Cklf	A	T	8: 104,263,364	K143*	probably null	Het
Clca3a1	A	G	3: 144,751,962	I387T	probably damaging	Het
Cndp1	T	A	18: 84,636,049	D130V	probably benign	Het
Cyp4a12a	T	A	4: 115,327,473	M317K	possibly damaging	Het
D13Ertd608e	A	T	13: 119,842,688		probably null	Het
Dmxl2	A	T	9: 54,457,794	I138K	unknown	Het
Eapp	A	G	12: 54,673,723	S236P	probably benign	Het
Efnb3	T	C	11: 69,557,220	H132R	possibly damaging	Het
Fbn2	A	T	18: 58,105,136	N596K	probably damaging	Het
Fzd9	A	G	5: 135,250,630	W134R	probably damaging	Het
Gga2	A	G	7: 122,003,934	S180P	probably damaging	Het
Gm11639	T	A	11: 105,036,799	M4798K	probably benign	Het
Gm30302	G	T	13: 49,786,975	Q420K	probably benign	Het
Golga3	A	G	5: 110,205,828	T911A	probably benign	Het
Gstm1	C	T	3: 108,014,550		probably null	Het
Heg1	A	G	16: 33,727,497	T909A	probably damaging	Het
Herc2	C	T	7: 56,220,525	R4349W	probably damaging	Het
Herc2	T	C	7: 56,157,438	S2455P	probably benign	Het
Hivep2	T	G	10: 14,143,851	M2122R	possibly damaging	Het
Itga10	T	A	3: 96,657,391		probably null	Het
Kbtbd13	G	T	9: 65,391,323	C110*	probably null	Het
Krt78	T	C	15: 101,950,883	E293G	probably damaging	Het
Lgals3bp	C	T	11: 118,398,169	V110M	possibly damaging	Het
Lrp2	A	G	2: 69,477,008		probably null	Het
Lrrc14	A	G	15: 76,713,973	D301G	probably benign	Het
Lrrc71	C	A	3: 87,741,806	G352W	probably damaging	Het
Map3k9	A	G	12: 81,724,732	V694A	probably benign	Het
Mboat2	T	C	12: 24,939,326	S162P	probably damaging	Het
Miga1	C	T	3: 152,276,687	S584N	probably benign	Het
Mindy2	A	G	9: 70,616,859	Y403H	probably damaging	Het
Mmd	C	T	11: 90,276,757	A204V	possibly damaging	Het
Mta3	A	T	17: 83,800,143	Y262F	probably benign	Het
Ncoa7	G	A	10: 30,722,798	S43F	probably benign	Het
Nphp4	T	C	4: 152,554,534	V874A	probably benign	Het
Nsd1	A	G	13: 55,312,328	T2226A	probably benign	Het
Nt5dc1	T	C	10: 34,314,796	H302R	probably benign	Het
Odc1	A	G	12: 17,550,002	Y389C	probably damaging	Het
Olfr175-ps1	G	A	16: 58,824,595	T38I	probably damaging	Het
Olfr551	A	G	7: 102,587,918	I275T	probably damaging	Het
Olfr970	T	G	9: 39,819,893	F85V	probably damaging	Het
Pcdhb10	A	C	18: 37,412,312	Q147P	probably benign	Het
Pdcl2	A	C	5: 76,317,828	C182G	probably damaging	Het
Pigv	T	C	4: 133,665,451	D136G	possibly damaging	Het
Pramel5	T	C	4: 144,271,440	E411G	possibly damaging	Het
Prkce	T	C	17: 86,168,600	V3A	probably benign	Het
Pth1r	T	C	9: 110,722,393	N546S	probably benign	Het
Qrich2	C	T	11: 116,455,322	V1559I	probably benign	Het
Rbm20	A	C	19: 53,814,333	D424A	possibly damaging	Het
Rbm26	C	T	14: 105,150,848	D393N	probably damaging	Het
Sf3b3	G	A	8: 110,820,813	R728C	probably damaging	Het
Srcap	A	G	7: 127,538,748	N1090S	probably benign	Het
Syt4	A	T	18: 31,443,822	S160T	probably benign	Het
Tfap2e	C	T	4: 126,721,934	V236M	probably damaging	Het
Thsd4	G	A	9: 60,394,472	T180M	probably damaging	Het
Tlr4	T	A	4: 66,840,206	M412K	probably damaging	Het
Tmem67	T	A	4: 12,079,883	H136L	probably benign	Het
Tnpo2	T	A	8: 85,044,415	I110N	probably benign	Het
Trav13d-3	A	G	14: 53,033,413	M111V	probably benign	Het
Tubb3	A	T	8: 123,421,161	S278C	probably benign	Het
Ugcg	T	A	4: 59,220,299	F364Y	probably benign	Het
Usp17lc	T	C	7: 103,418,499	S334P	probably damaging	Het
Vps13c	A	G	9: 67,945,509	D2357G	probably damaging	Het
Zfp157	T	C	5: 138,455,910	Y125H	probably benign	Het
Zfp747	A	T	7: 127,374,647	M117K	probably benign	Het
Znrd1	A	C	17: 36,957,830		probably null	Het
Zscan4c	A	T	7: 11,009,731	N419I	possibly damaging	Het

Other mutations in Megf11

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00979:Megf11	APN	9	64508727	missense	probably damaging	1.00
IGL01285:Megf11	APN	9	64660446	missense	probably damaging	1.00
IGL01309:Megf11	APN	9	64681416	missense	probably benign	0.01
IGL01953:Megf11	APN	9	64690088	missense	probably damaging	1.00
IGL02341:Megf11	APN	9	64544620	missense	probably damaging	1.00
IGL02407:Megf11	APN	9	64680249	missense	probably damaging	1.00
IGL02621:Megf11	APN	9	64693932	missense	probably benign	0.07
R0277:Megf11	UTSW	9	64691350	critical splice donor site	probably null
R0386:Megf11	UTSW	9	64640078	missense	probably damaging	1.00
R1354:Megf11	UTSW	9	64653177	missense	probably benign	0.00
R1709:Megf11	UTSW	9	64695412	missense	probably damaging	1.00
R1865:Megf11	UTSW	9	64680299	missense	probably benign	0.39
R1895:Megf11	UTSW	9	64679276	missense	probably damaging	1.00
R1946:Megf11	UTSW	9	64679276	missense	probably damaging	1.00
R2221:Megf11	UTSW	9	64660431	missense	possibly damaging	0.63
R2223:Megf11	UTSW	9	64660431	missense	possibly damaging	0.63
R3552:Megf11	UTSW	9	64695463	missense	possibly damaging	0.75
R4641:Megf11	UTSW	9	64690125	missense	possibly damaging	0.93
R4746:Megf11	UTSW	9	64508745	missense	probably damaging	1.00
R5594:Megf11	UTSW	9	64686473	missense	probably damaging	1.00
R5716:Megf11	UTSW	9	64506110	missense	possibly damaging	0.72
R5898:Megf11	UTSW	9	64685964	missense	probably damaging	1.00
R5960:Megf11	UTSW	9	64660449	missense	probably benign	0.00
R6372:Megf11	UTSW	9	64706625	missense	probably damaging	1.00
R6811:Megf11	UTSW	9	64544641	missense	probably damaging	0.99
R6868:Megf11	UTSW	9	64680309	missense	probably damaging	1.00
R6980:Megf11	UTSW	9	64705850	missense	probably damaging	1.00
R6984:Megf11	UTSW	9	64686452	missense	probably benign
R7155:Megf11	UTSW	9	64647951	missense	probably null	1.00
R7643:Megf11	UTSW	9	64706632	missense	probably damaging	1.00
R7688:Megf11	UTSW	9	64691864	missense	possibly damaging	0.92
R7840:Megf11	UTSW	9	64695427	missense	possibly damaging	0.94
R8744:Megf11	UTSW	9	64544688	critical splice donor site	probably null
R8799:Megf11	UTSW	9	64681391	missense	probably benign	0.05
R9383:Megf11	UTSW	9	64638450	missense	probably damaging	1.00
R9493:Megf11	UTSW	9	64640094	missense	probably damaging	1.00
R9797:Megf11	UTSW	9	64638309	missense	possibly damaging	0.83
V5088:Megf11	UTSW	9	64690069	nonsense	probably null
V5622:Megf11	UTSW	9	64690069	nonsense	probably null
V5622:Megf11	UTSW	9	64690069	nonsense	probably null
Z1088:Megf11	UTSW	9	64660476	missense	probably damaging	1.00
Z1177:Megf11	UTSW	9	64680326	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGGTTGGAAGCATGGACATC -3'
(R):5'- ATTGTCCCTGCAGACCATG -3'

Sequencing Primer
(F):5'- CATGGACATCAGGAGGAGCACC -3'
(R):5'- TGCAGACCATGTGGCTCAC -3'

Posted On

2019-10-24