|Institutional Source||Beutler Lab|
|Gene Name||engrailed 2|
|Essential gene?||Probably essential (E-score: 0.804)|
|Stock #||R7640 (G1)|
|Chromosomal Location||28165694-28172166 bp(+) (GRCm38)|
|Type of Mutation||nonsense|
|DNA Base Change (assembly)||A to T at 28170166 bp (GRCm38)|
|Amino Acid Change||Lysine to Stop codon at position 236 (K236*)|
|Ref Sequence||ENSEMBL: ENSMUSP00000036761 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000036177]|
AA Change: K236*
AA Change: K236*
|Coding Region Coverage||
|Validation Efficiency||99% (68/69)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]
PHENOTYPE: This locus affects anterior-posterior cerebellar patterning. Homozygous null mutants show altered foliation pattern and perform poorly in motor learning (rotarod) tests. Heterozygotes test intermediate on rotarod. Hypomorphs show no phenotypic effects. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in En2||
(F):5'- CAATGCCCAACACTTGTATGG -3'
(R):5'- GCACCGCCAAAGTGTTCTTG -3'
(F):5'- CCAACACTTGTATGGCCTTGGG -3'
(R):5'- GCCAAAGTGTTCTTGTTGCC -3'