Incidental Mutation 'R7643:Gpr15'
ID590380
Institutional Source Beutler Lab
Gene Symbol Gpr15
Ensembl Gene ENSMUSG00000047293
Gene NameG protein-coupled receptor 15
Synonyms4933439K08Rik
MMRRC Submission
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R7643 (G1)
Quality Score225.009
Status Validated
Chromosome16
Chromosomal Location58717433-58719070 bp(-) (GRCm38)
Type of Mutationnonsense
DNA Base Change (assembly) A to C at 58717816 bp
ZygosityHeterozygous
Amino Acid Change Tyrosine to Stop codon at position 303 (Y303*)
Ref Sequence ENSEMBL: ENSMUSP00000086731 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000089318]
Predicted Effect probably null
Transcript: ENSMUST00000089318
AA Change: Y303*
SMART Domains Protein: ENSMUSP00000086731
Gene: ENSMUSG00000047293
AA Change: Y303*

DomainStartEndE-ValueType
Pfam:7tm_1 50 302 1.3e-46 PFAM
Pfam:7TM_GPCR_Srv 66 317 7.1e-9 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency 100% (64/64)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a G protein-coupled receptor that acts as a chemokine receptor for human immunodeficiency virus type 1 and 2. The encoded protein localizes to the cell membrane. [provided by RefSeq, Nov 2012]
PHENOTYPE: Mice homozygous for a a knock-out allele exhibit impaired regulatory T cell homing in the large intestine mucosa. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 64 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930435E12Rik A G 16: 38,827,863 Y295H probably benign Het
4931406C07Rik A G 9: 15,297,860 F46S probably damaging Het
Acaca T C 11: 84,338,356 Y1670H probably damaging Het
Acrbp G A 6: 125,053,832 R272Q possibly damaging Het
Adcy6 T A 15: 98,593,568 Q1050L probably benign Het
Amn1 C T 6: 149,185,031 M44I probably benign Het
Ankrd13b A G 11: 77,473,085 V395A probably benign Het
Ap3b2 T C 7: 81,477,072 K310R probably benign Het
Bnc2 T C 4: 84,506,574 D123G probably benign Het
Bst1 G A 5: 43,840,449 M263I probably benign Het
Ccdc7a C T 8: 128,889,811 G937E probably damaging Het
Cep290 A G 10: 100,537,553 M1232V probably benign Het
Cfhr1 A G 1: 139,553,585 Y186H possibly damaging Het
Dnah7c A T 1: 46,602,813 H1203L probably benign Het
Emc7 A G 2: 112,455,279 E71G probably benign Het
Exoc3l4 G A 12: 111,421,935 probably benign Het
Fam83c A G 2: 155,831,004 F278L possibly damaging Het
Gabpb2 C A 3: 95,200,225 V180L probably benign Het
Gbp2b G T 3: 142,603,609 Q160H probably benign Het
Gm19965 C G 1: 116,822,229 Q547E unknown Het
Gm40460 ACCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG ACCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG 7: 142,240,713 probably benign Het
Gon4l T A 3: 88,902,807 D1774E probably damaging Het
Greb1 T C 12: 16,711,996 D461G probably damaging Het
Gria4 T C 9: 4,793,950 N36S probably benign Het
Hacd1 T C 2: 14,044,791 I119V probably damaging Het
Ing5 T A 1: 93,812,433 D101E probably damaging Het
Irak4 T A 15: 94,558,828 N297K probably benign Het
Itga7 A G 10: 128,953,501 D971G probably benign Het
Klf5 A G 14: 99,313,178 E397G possibly damaging Het
Krtap29-1 C T 11: 99,978,198 G286S probably damaging Het
Lrp2bp A T 8: 46,020,527 probably null Het
March4 T G 1: 72,447,220 Q266H probably damaging Het
Med23 A G 10: 24,905,965 T1056A probably benign Het
Megf11 T C 9: 64,706,632 L1079P probably damaging Het
Mycbp2 G T 14: 103,346,265 L85I probably benign Het
Nlgn2 G T 11: 69,827,885 Q290K probably damaging Het
Nox4 A T 7: 87,323,754 E323V probably damaging Het
Nup93 T C 8: 94,286,619 probably null Het
Olfr298 C T 7: 86,489,568 probably null Het
Olfr558 C T 7: 102,709,538 T93I probably benign Het
Olfr977-ps1 T A 9: 39,974,821 M1L unknown Het
Otop3 T C 11: 115,339,648 L117P probably damaging Het
Pde6c C A 19: 38,141,421 Q260K probably damaging Het
Plb1 C T 5: 32,247,557 Q20* probably null Het
Qser1 A T 2: 104,786,977 Y1163* probably null Het
Rbm12 A T 2: 156,098,217 I45N unknown Het
Rictor T A 15: 6,769,269 Y332* probably null Het
Rpp14 C A 14: 8,090,325 S83* probably null Het
Sel1l3 C T 5: 53,123,162 probably null Het
Setd2 T C 9: 110,567,840 probably null Het
Spink5 A G 18: 44,010,252 T759A probably benign Het
Spon2 T A 5: 33,217,456 E2V probably benign Het
Tdrd1 T C 19: 56,837,708 S144P probably damaging Het
Tex15 A C 8: 33,575,120 Y1526S probably damaging Het
Tnfrsf21 T C 17: 43,037,916 S140P probably benign Het
Trav6-2 T A 14: 52,667,442 M11K probably benign Het
Trp53bp1 G T 2: 121,247,814 probably null Het
Ttn T C 2: 76,734,827 N28352S possibly damaging Het
Uckl1 T C 2: 181,573,106 I292V probably benign Het
Unc13b T A 4: 43,216,333 S211T probably benign Het
Zcchc4 T C 5: 52,808,293 I313T possibly damaging Het
Zfp106 C T 2: 120,512,734 R1811K probably benign Het
Zfp599 G T 9: 22,249,892 Q326K probably benign Het
Zscan4e A T 7: 11,309,525 M108K probably damaging Het
Other mutations in Gpr15
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00473:Gpr15 APN 16 58718078 missense probably damaging 0.99
IGL02616:Gpr15 APN 16 58718204 missense probably damaging 1.00
IGL03381:Gpr15 APN 16 58717976 missense probably damaging 1.00
PIT4418001:Gpr15 UTSW 16 58717950 missense probably benign 0.13
R1484:Gpr15 UTSW 16 58718574 missense probably damaging 1.00
R1775:Gpr15 UTSW 16 58718558 missense probably benign 0.05
R1959:Gpr15 UTSW 16 58718007 missense probably benign 0.03
R1961:Gpr15 UTSW 16 58718007 missense probably benign 0.03
R2127:Gpr15 UTSW 16 58718255 missense possibly damaging 0.67
R3825:Gpr15 UTSW 16 58718360 missense probably damaging 1.00
R4957:Gpr15 UTSW 16 58718174 missense probably damaging 0.99
R5098:Gpr15 UTSW 16 58718527 missense probably damaging 1.00
R5180:Gpr15 UTSW 16 58717885 missense probably benign 0.07
R5668:Gpr15 UTSW 16 58717650 missense probably damaging 1.00
R6104:Gpr15 UTSW 16 58717976 missense probably damaging 1.00
R6281:Gpr15 UTSW 16 58718594 missense probably damaging 1.00
R6921:Gpr15 UTSW 16 58717781 missense probably benign 0.00
R6980:Gpr15 UTSW 16 58718742 start gained probably benign
R6981:Gpr15 UTSW 16 58718185 missense probably benign 0.44
R7252:Gpr15 UTSW 16 58718397 missense probably damaging 1.00
R7680:Gpr15 UTSW 16 58717965 missense probably damaging 1.00
R7844:Gpr15 UTSW 16 58718510 missense probably damaging 1.00
R7954:Gpr15 UTSW 16 58718684 missense probably benign 0.00
R8100:Gpr15 UTSW 16 58717713 missense probably benign 0.00
R8372:Gpr15 UTSW 16 58718487 missense probably benign 0.09
Predicted Primers PCR Primer
(F):5'- GACACAGATCTCTTCCTCCG -3'
(R):5'- CATTACCAGCAGTCGGGAAAG -3'

Sequencing Primer
(F):5'- TCCTCCGCCGGATGAAATC -3'
(R):5'- ATAAAGATCGTTATTATTGCGGTGGC -3'
Posted On2019-10-24