Incidental Mutation 'R7645:Srgn'
ID 590488
Institutional Source Beutler Lab
Gene Symbol Srgn
Ensembl Gene ENSMUSG00000020077
Gene Name serglycin
Synonyms Sgc, Prg1
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.109) question?
Stock # R7645 (G1)
Quality Score 225.009
Status Validated
Chromosome 10
Chromosomal Location 62493833-62527451 bp(-) (GRCm38)
Type of Mutation nonsense
DNA Base Change (assembly) C to T at 62494978 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Tryptophan to Stop codon at position 116 (W116*)
Ref Sequence ENSEMBL: ENSMUSP00000020271 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000020271] [ENSMUST00000160643] [ENSMUST00000160987] [ENSMUST00000162161]
AlphaFold P13609
Predicted Effect probably null
Transcript: ENSMUST00000020271
AA Change: W116*
SMART Domains Protein: ENSMUSP00000020271
Gene: ENSMUSG00000020077
AA Change: W116*

DomainStartEndE-ValueType
Pfam:Serglycin 1 151 9e-75 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000160643
Predicted Effect probably null
Transcript: ENSMUST00000160987
AA Change: W116*
SMART Domains Protein: ENSMUSP00000125622
Gene: ENSMUSG00000020077
AA Change: W116*

DomainStartEndE-ValueType
Pfam:Serglycin 1 149 8.3e-70 PFAM
Predicted Effect probably null
Transcript: ENSMUST00000162161
AA Change: W116*
SMART Domains Protein: ENSMUSP00000125533
Gene: ENSMUSG00000020077
AA Change: W116*

DomainStartEndE-ValueType
Pfam:Serglycin 1 151 9e-75 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.2%
Validation Efficiency 98% (46/47)
MGI Phenotype PHENOTYPE: Mice homozygous for disruptions in this gene lack peritoneal mast cells. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 47 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Actbl2 T A 13: 111,256,255 C375S probably benign Het
Adgrg3 A G 8: 95,034,764 probably benign Het
Amn1 C T 6: 149,185,031 M44I probably benign Het
Atg2b T C 12: 105,623,430 Y1854C probably benign Het
Bcat2 T A 7: 45,587,963 N290K probably benign Het
Ccdc39 T C 3: 33,825,169 probably null Het
Cnot10 T C 9: 114,613,637 S501G probably benign Het
Col6a6 A G 9: 105,767,198 probably null Het
Coq2 A C 5: 100,660,250 C228W probably damaging Het
Cpt2 A T 4: 107,906,974 M531K possibly damaging Het
Dennd1a A G 2: 38,021,363 L204P probably damaging Het
Dirc2 T A 16: 35,734,068 probably null Het
Dock9 C T 14: 121,597,663 V1305M probably benign Het
Dusp16 A G 6: 134,725,925 I201T probably damaging Het
Esco2 T C 14: 65,827,181 D370G probably benign Het
Fbxl4 T C 4: 22,377,037 S158P probably damaging Het
Fut10 A G 8: 31,236,204 H329R possibly damaging Het
Gm10436 T C 12: 88,176,258 T392A probably damaging Het
Gpr3 A G 4: 133,211,329 W11R probably damaging Het
H2-M3 T A 17: 37,270,729 I94N probably damaging Het
Krt7 T C 15: 101,412,643 I57T probably damaging Het
Ltc4s T C 11: 50,238,546 probably benign Het
Ms4a6b A T 19: 11,523,940 T105S probably damaging Het
Muc6 T C 7: 141,648,658 H592R probably benign Het
Ncam1 T C 9: 49,565,003 E262G probably benign Het
Olfr1265 T A 2: 90,037,747 I276K possibly damaging Het
Olfr1462 T C 19: 13,191,573 V302A probably benign Het
Plxnb1 T A 9: 109,114,412 S1908T probably damaging Het
Polg2 A T 11: 106,775,593 M242K probably benign Het
Polr3g T C 13: 81,694,444 T151A unknown Het
Psmb8 T C 17: 34,200,212 L160P possibly damaging Het
Rab5b G A 10: 128,681,391 A176V possibly damaging Het
Rreb1 T C 13: 37,931,034 C790R probably damaging Het
Rufy3 A G 5: 88,640,617 T506A probably benign Het
Samd11 T C 4: 156,255,183 probably benign Het
Slc12a2 T C 18: 57,896,378 F279L possibly damaging Het
Son TCCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCAGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG TCCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG 16: 91,660,295 probably benign Het
Svil A G 18: 5,099,663 E1733G probably damaging Het
Tbc1d22a C T 15: 86,235,541 P49S probably benign Het
Tbc1d9 A T 8: 83,242,553 K490M probably damaging Het
Tcte1 A G 17: 45,534,989 N173S probably benign Het
Tmco6 C T 18: 36,735,393 R31W probably damaging Het
Ttn C A 2: 76,899,681 A5155S unknown Het
Tyro3 T C 2: 119,816,906 Y839H probably damaging Het
Zc3h12d A G 10: 7,867,576 D370G probably benign Het
Zc3h7b T C 15: 81,780,602 L554P probably damaging Het
Zfp352 C T 4: 90,224,777 P385S probably benign Het
Other mutations in Srgn
AlleleSourceChrCoordTypePredicted EffectPPH Score
BB008:Srgn UTSW 10 62494984 missense possibly damaging 0.71
BB018:Srgn UTSW 10 62494984 missense possibly damaging 0.71
R2120:Srgn UTSW 10 62507634 splice site probably benign
R3704:Srgn UTSW 10 62497830 missense probably damaging 0.99
R4155:Srgn UTSW 10 62497834 missense possibly damaging 0.92
R4156:Srgn UTSW 10 62497834 missense possibly damaging 0.92
R4157:Srgn UTSW 10 62497834 missense possibly damaging 0.92
R4782:Srgn UTSW 10 62497852 missense possibly damaging 0.95
R5169:Srgn UTSW 10 62495087 missense probably damaging 1.00
R7595:Srgn UTSW 10 62508006 unclassified probably benign
R7931:Srgn UTSW 10 62494984 missense possibly damaging 0.71
R8292:Srgn UTSW 10 62507668 missense possibly damaging 0.94
R8324:Srgn UTSW 10 62507665 missense probably damaging 0.99
Predicted Primers PCR Primer
(F):5'- TGAGACTGGTCATTTAAACGTGG -3'
(R):5'- GCAATACTTTGTGTGTACTCCAC -3'

Sequencing Primer
(F):5'- CAGAGACCGTCACATTCA -3'
(R):5'- TGTACTCCACACCTAGTGTATTTC -3'
Posted On 2019-10-24