Mutagenetix > Incidental Mutations

Incidental Mutation 'R7645:Polr3g'

590495

Institutional Source

Beutler Lab

Gene Symbol

Polr3g

Ensembl Gene

ENSMUSG00000035834

Gene Name

polymerase (RNA) III (DNA directed) polypeptide G

Synonyms

RPC32, 2310047G20Rik

MMRRC Submission

Accession Numbers

Is this an essential gene?

Essential (E-score: 1.000) question?

Stock #

R7645 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

81673843-81711013 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 81694444 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 151 (T151A)

Ref Sequence

ENSEMBL: ENSMUSP00000125054 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000048993] [ENSMUST00000161920]

AlphaFold

Q6NXY9

Predicted Effect

probably benign
Transcript: ENSMUST00000048993

SMART Domains

Protein: ENSMUSP00000035289
Gene: ENSMUSG00000035834

Domain	Start	End	E-Value	Type
Pfam:RNA_pol_3_Rpc31	1	223	7e-41	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000161920
AA Change: T151A

SMART Domains

Protein: ENSMUSP00000125054
Gene: ENSMUSG00000035834
AA Change: T151A

Domain	Start	End	E-Value	Type
Pfam:RNA_pol_3_Rpc31	1	105	1e-13	PFAM
transmembrane domain	136	158	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

98% (46/47)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actbl2	T	A	13: 111,256,255	C375S	probably benign	Het
Adgrg3	A	G	8: 95,034,764		probably benign	Het
Amn1	C	T	6: 149,185,031	M44I	probably benign	Het
Atg2b	T	C	12: 105,623,430	Y1854C	probably benign	Het
Bcat2	T	A	7: 45,587,963	N290K	probably benign	Het
Ccdc39	T	C	3: 33,825,169		probably null	Het
Cnot10	T	C	9: 114,613,637	S501G	probably benign	Het
Col6a6	A	G	9: 105,767,198		probably null	Het
Coq2	A	C	5: 100,660,250	C228W	probably damaging	Het
Cpt2	A	T	4: 107,906,974	M531K	possibly damaging	Het
Dennd1a	A	G	2: 38,021,363	L204P	probably damaging	Het
Dirc2	T	A	16: 35,734,068		probably null	Het
Dock9	C	T	14: 121,597,663	V1305M	probably benign	Het
Dusp16	A	G	6: 134,725,925	I201T	probably damaging	Het
Esco2	T	C	14: 65,827,181	D370G	probably benign	Het
Fbxl4	T	C	4: 22,377,037	S158P	probably damaging	Het
Fut10	A	G	8: 31,236,204	H329R	possibly damaging	Het
Gm10436	T	C	12: 88,176,258	T392A	probably damaging	Het
Gpr3	A	G	4: 133,211,329	W11R	probably damaging	Het
H2-M3	T	A	17: 37,270,729	I94N	probably damaging	Het
Krt7	T	C	15: 101,412,643	I57T	probably damaging	Het
Ltc4s	T	C	11: 50,238,546		probably benign	Het
Ms4a6b	A	T	19: 11,523,940	T105S	probably damaging	Het
Muc6	T	C	7: 141,648,658	H592R	probably benign	Het
Ncam1	T	C	9: 49,565,003	E262G	probably benign	Het
Olfr1265	T	A	2: 90,037,747	I276K	possibly damaging	Het
Olfr1462	T	C	19: 13,191,573	V302A	probably benign	Het
Plxnb1	T	A	9: 109,114,412	S1908T	probably damaging	Het
Polg2	A	T	11: 106,775,593	M242K	probably benign	Het
Psmb8	T	C	17: 34,200,212	L160P	possibly damaging	Het
Rab5b	G	A	10: 128,681,391	A176V	possibly damaging	Het
Rreb1	T	C	13: 37,931,034	C790R	probably damaging	Het
Rufy3	A	G	5: 88,640,617	T506A	probably benign	Het
Samd11	T	C	4: 156,255,183		probably benign	Het
Slc12a2	T	C	18: 57,896,378	F279L	possibly damaging	Het
Son	TCCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCAGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG	TCCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG	16: 91,660,295		probably benign	Het
Srgn	C	T	10: 62,494,978	W116*	probably null	Het
Svil	A	G	18: 5,099,663	E1733G	probably damaging	Het
Tbc1d22a	C	T	15: 86,235,541	P49S	probably benign	Het
Tbc1d9	A	T	8: 83,242,553	K490M	probably damaging	Het
Tcte1	A	G	17: 45,534,989	N173S	probably benign	Het
Tmco6	C	T	18: 36,735,393	R31W	probably damaging	Het
Ttn	C	A	2: 76,899,681	A5155S	unknown	Het
Tyro3	T	C	2: 119,816,906	Y839H	probably damaging	Het
Zc3h12d	A	G	10: 7,867,576	D370G	probably benign	Het
Zc3h7b	T	C	15: 81,780,602	L554P	probably damaging	Het
Zfp352	C	T	4: 90,224,777	P385S	probably benign	Het

Other mutations in Polr3g

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00886:Polr3g	APN	13	81694677	missense	probably damaging	1.00
IGL01764:Polr3g	APN	13	81682119	missense	possibly damaging	0.93
R0118:Polr3g	UTSW	13	81676121	unclassified	probably benign
R0550:Polr3g	UTSW	13	81694773	missense	probably damaging	0.99
R3725:Polr3g	UTSW	13	81694635	missense	probably damaging	0.99
R6536:Polr3g	UTSW	13	81678216	missense	unknown
R6684:Polr3g	UTSW	13	81699531	splice site	probably null
R8296:Polr3g	UTSW	13	81694563	missense	unknown
R8317:Polr3g	UTSW	13	81678183	missense	unknown
R9178:Polr3g	UTSW	13	81694416	missense	unknown

Predicted Primers

PCR Primer
(F):5'- AAACTGTCAATAATCAGAGGCATCC -3'
(R):5'- TGGCAGTTCAGTGTTCACAG -3'

Sequencing Primer
(F):5'- TGGCAGTAATCACAGCATTGC -3'
(R):5'- TCATGTGACTCAGCCGAT -3'

Posted On

2019-10-24