Mutagenetix > Incidental Mutation

Incidental Mutation 'R7649:Mical3'

590690

Institutional Source

Beutler Lab

Gene Symbol

Mical3

Ensembl Gene

ENSMUSG00000051586

Gene Name

microtubule associated monooxygenase, calponin and LIM domain containing 3

Synonyms

C130040D16Rik, MICAL-3

MMRRC Submission

045646-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.163) question?

Stock #

R7649 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

120908668-121107959 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 120911909 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Tryptophan at position 1928 (R1928W)

Ref Sequence

ENSEMBL: ENSMUSP00000146544 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000207889]

AlphaFold

Q8CJ19

Predicted Effect

probably damaging
Transcript: ENSMUST00000207889
AA Change: R1928W

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Predicted Effect

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

98% (57/58)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actr3b	T	C	5: 26,053,364 (GRCm39)	V319A	probably benign	Het
Adam28	T	A	14: 68,872,282 (GRCm39)	Y320F	probably benign	Het
Adam4	T	C	12: 81,467,151 (GRCm39)	Y490C	probably damaging	Het
Ampd3	G	T	7: 110,377,049 (GRCm39)	L13F	probably benign	Het
Arhgap45	C	A	10: 79,866,835 (GRCm39)	Q1113K	probably benign	Het
Arid5b	T	C	10: 67,954,175 (GRCm39)	T370A	possibly damaging	Het
Arl15	A	G	13: 114,104,208 (GRCm39)	D115G	possibly damaging	Het
AW146154	G	A	7: 41,130,156 (GRCm39)	T320I	probably benign	Het
Cdh17	C	T	4: 11,814,698 (GRCm39)	P751L	probably damaging	Het
Chchd4	C	T	6: 91,444,754 (GRCm39)	R11Q	probably benign	Het
Clta	T	G	4: 44,025,494 (GRCm39)	I140R	possibly damaging	Het
Cnpy1	T	C	5: 28,412,282 (GRCm39)	T84A	probably benign	Het
Cpne5	T	C	17: 29,445,172 (GRCm39)	D44G	probably damaging	Het
Crkl	T	C	16: 17,270,366 (GRCm39)	S9P	unknown	Het
Csmd3	T	C	15: 47,532,539 (GRCm39)	D1641G		Het
Ctnnd2	A	G	15: 31,027,630 (GRCm39)	T1185A	probably benign	Het
Dlc1	A	T	8: 37,049,894 (GRCm39)	L607Q	probably damaging	Het
Dpy19l2	T	C	9: 24,607,459 (GRCm39)	M1V	probably null	Het
Dst	T	C	1: 34,206,778 (GRCm39)	V982A	probably benign	Het
Fam135b	T	C	15: 71,334,429 (GRCm39)	I922V	probably benign	Het
Fbxl13	T	C	5: 21,819,664 (GRCm39)	E245G	probably benign	Het
Fcgbp	T	C	7: 27,790,928 (GRCm39)	S730P	possibly damaging	Het
Ggt1	T	C	10: 75,421,290 (GRCm39)	V468A	possibly damaging	Het
Gja5	A	T	3: 96,958,957 (GRCm39)	H338L	probably benign	Het
Herpud2	T	C	9: 25,021,902 (GRCm39)	E248G	possibly damaging	Het
Il17rd	C	A	14: 26,761,167 (GRCm39)	A36E	probably benign	Het
Irf2bpl	T	C	12: 86,929,572 (GRCm39)	Y367C	possibly damaging	Het
Lama1	T	A	17: 68,044,549 (GRCm39)	D149E		Het
Lipf	A	T	19: 33,943,098 (GRCm39)	E135D	possibly damaging	Het
Manea	T	A	4: 26,328,234 (GRCm39)	D269V	probably damaging	Het
Megf6	A	G	4: 154,349,542 (GRCm39)	Y910C	probably damaging	Het
Or13c3	G	A	4: 52,855,692 (GRCm39)	Q274*	probably null	Het
Or5b108	T	A	19: 13,168,136 (GRCm39)	I35N	possibly damaging	Het
Plch1	G	T	3: 63,605,590 (GRCm39)	S1438*	probably null	Het
Por	T	C	5: 135,763,359 (GRCm39)	F595L	probably damaging	Het
Prkcg	G	A	7: 3,378,480 (GRCm39)	R634H	probably benign	Het
Prr14l	C	T	5: 32,985,589 (GRCm39)	G1302D	probably benign	Het
Rcan3	A	T	4: 135,139,799 (GRCm39)	S135T	probably benign	Het
Rnf223	A	G	4: 156,216,660 (GRCm39)	M12V	probably benign	Het
Rps6kb2	A	G	19: 4,207,020 (GRCm39)	S483P	unknown	Het
Sacs	T	A	14: 61,440,677 (GRCm39)	F908I	possibly damaging	Het
Slc4a9	A	G	18: 36,661,430 (GRCm39)	Q64R	probably benign	Het
Slco4c1	T	A	1: 96,756,667 (GRCm39)	I552F	probably benign	Het
Sppl2b	C	T	10: 80,703,253 (GRCm39)	P505L	probably benign	Het
Sptbn4	A	G	7: 27,061,002 (GRCm39)	S2434P	possibly damaging	Het
Ssh1	A	T	5: 114,088,612 (GRCm39)	M395K	probably benign	Het
Stk10	A	T	11: 32,527,764 (GRCm39)	I171F		Het
Tmx3	C	T	18: 90,558,154 (GRCm39)	A402V	probably damaging	Het
Tnfrsf13c	T	C	15: 82,108,341 (GRCm39)	D58G	possibly damaging	Het
Tnk1	T	C	11: 69,744,403 (GRCm39)		probably null	Het
Trav14n-3	A	T	14: 53,607,951 (GRCm39)	N94Y	probably damaging	Het
Trav5-4	A	T	14: 53,941,902 (GRCm39)	K92*	probably null	Het
Tsga10	C	T	1: 37,874,229 (GRCm39)	R190H	unknown	Het
Zfhx3	T	C	8: 109,678,276 (GRCm39)	F3109L	probably benign	Het
Zfhx4	A	G	3: 5,307,170 (GRCm39)	Y132C	probably damaging	Het
Zfp521	T	C	18: 13,977,413 (GRCm39)	E1000G	probably damaging	Het

Other mutations in Mical3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00569:Mical3	APN	6	120,938,585 (GRCm39)	missense	possibly damaging	0.73
IGL00718:Mical3	APN	6	121,017,410 (GRCm39)	missense	probably damaging	0.98
IGL00940:Mical3	APN	6	120,999,371 (GRCm39)	missense	possibly damaging	0.55
IGL00973:Mical3	APN	6	120,911,885 (GRCm39)	splice site	probably benign
IGL01503:Mical3	APN	6	120,935,537 (GRCm39)	missense	probably benign	0.09
IGL01991:Mical3	APN	6	120,912,172 (GRCm39)	missense	probably damaging	0.98
IGL02794:Mical3	APN	6	120,984,270 (GRCm39)	missense	probably damaging	0.99
IGL02996:Mical3	APN	6	120,935,519 (GRCm39)	missense	probably damaging	1.00
IGL03105:Mical3	APN	6	121,019,199 (GRCm39)	missense	probably benign	0.01
IGL03109:Mical3	APN	6	120,986,085 (GRCm39)	missense	probably damaging	1.00
IGL03236:Mical3	APN	6	120,946,345 (GRCm39)	missense	probably benign	0.00
P0028:Mical3	UTSW	6	121,001,650 (GRCm39)	missense	probably benign	0.33
R0244:Mical3	UTSW	6	120,934,683 (GRCm39)	missense	probably benign	0.00
R0494:Mical3	UTSW	6	120,936,162 (GRCm39)	missense	possibly damaging	0.94
R0586:Mical3	UTSW	6	121,006,602 (GRCm39)	unclassified	probably benign
R1029:Mical3	UTSW	6	120,911,639 (GRCm39)	missense	probably benign	0.02
R1263:Mical3	UTSW	6	120,929,430 (GRCm39)	missense	probably damaging	0.99
R1507:Mical3	UTSW	6	121,019,199 (GRCm39)	missense	probably benign	0.36
R1527:Mical3	UTSW	6	121,001,740 (GRCm39)	missense	probably damaging	0.99
R1623:Mical3	UTSW	6	121,001,768 (GRCm39)	missense	probably damaging	0.99
R1680:Mical3	UTSW	6	120,936,604 (GRCm39)	missense	probably benign	0.09
R1697:Mical3	UTSW	6	120,984,369 (GRCm39)	missense	possibly damaging	0.84
R1817:Mical3	UTSW	6	121,019,196 (GRCm39)	missense	probably benign	0.06
R1875:Mical3	UTSW	6	121,019,025 (GRCm39)	missense	probably damaging	1.00
R1961:Mical3	UTSW	6	120,959,568 (GRCm39)	missense	possibly damaging	0.94
R2004:Mical3	UTSW	6	120,928,283 (GRCm39)	missense	probably damaging	1.00
R2093:Mical3	UTSW	6	121,017,347 (GRCm39)	missense	probably damaging	1.00
R2141:Mical3	UTSW	6	121,008,095 (GRCm39)	splice site	probably null
R2142:Mical3	UTSW	6	121,008,095 (GRCm39)	splice site	probably null
R2257:Mical3	UTSW	6	121,010,696 (GRCm39)	missense	possibly damaging	0.94
R2404:Mical3	UTSW	6	120,936,789 (GRCm39)	missense	probably benign	0.01
R2419:Mical3	UTSW	6	120,936,884 (GRCm39)	missense	probably benign
R2509:Mical3	UTSW	6	121,011,118 (GRCm39)	missense	probably damaging	1.00
R3784:Mical3	UTSW	6	120,998,298 (GRCm39)	missense	probably benign	0.00
R4342:Mical3	UTSW	6	120,911,799 (GRCm39)	nonsense	probably null
R4343:Mical3	UTSW	6	120,911,799 (GRCm39)	nonsense	probably null
R4579:Mical3	UTSW	6	120,935,660 (GRCm39)	missense	probably benign
R4603:Mical3	UTSW	6	120,911,799 (GRCm39)	nonsense	probably null
R4605:Mical3	UTSW	6	121,011,041 (GRCm39)	nonsense	probably null
R4610:Mical3	UTSW	6	120,911,799 (GRCm39)	nonsense	probably null
R4611:Mical3	UTSW	6	120,911,799 (GRCm39)	nonsense	probably null
R4623:Mical3	UTSW	6	120,938,586 (GRCm39)	nonsense	probably null
R4669:Mical3	UTSW	6	120,934,664 (GRCm39)	missense	probably damaging	0.98
R4704:Mical3	UTSW	6	120,935,649 (GRCm39)	missense	probably benign	0.00
R4722:Mical3	UTSW	6	121,015,486 (GRCm39)	missense	probably benign	0.00
R4863:Mical3	UTSW	6	121,010,748 (GRCm39)	missense	probably damaging	0.99
R4878:Mical3	UTSW	6	120,946,348 (GRCm39)	missense	possibly damaging	0.51
R4885:Mical3	UTSW	6	120,912,214 (GRCm39)	missense	probably damaging	1.00
R4907:Mical3	UTSW	6	120,984,259 (GRCm39)	missense	probably benign	0.00
R5007:Mical3	UTSW	6	121,015,030 (GRCm39)	missense	probably damaging	0.98
R5299:Mical3	UTSW	6	120,936,473 (GRCm39)	missense	possibly damaging	0.71
R5303:Mical3	UTSW	6	120,936,941 (GRCm39)	missense	probably benign
R5368:Mical3	UTSW	6	120,936,434 (GRCm39)	missense	probably damaging	1.00
R5955:Mical3	UTSW	6	121,010,711 (GRCm39)	missense	probably damaging	0.99
R5970:Mical3	UTSW	6	120,935,232 (GRCm39)	nonsense	probably null
R6000:Mical3	UTSW	6	120,998,281 (GRCm39)	missense	probably benign	0.06
R6101:Mical3	UTSW	6	121,010,671 (GRCm39)	missense	probably damaging	1.00
R6195:Mical3	UTSW	6	120,993,796 (GRCm39)	intron	probably benign
R6210:Mical3	UTSW	6	121,017,478 (GRCm39)	splice site	probably null
R6225:Mical3	UTSW	6	120,935,684 (GRCm39)	missense	probably damaging	0.98
R6258:Mical3	UTSW	6	120,985,991 (GRCm39)	missense	probably damaging	1.00
R6260:Mical3	UTSW	6	120,985,991 (GRCm39)	missense	probably damaging	1.00
R6349:Mical3	UTSW	6	120,936,486 (GRCm39)	missense	probably benign
R6352:Mical3	UTSW	6	120,929,434 (GRCm39)	missense	probably damaging	0.97
R6480:Mical3	UTSW	6	121,011,236 (GRCm39)	missense	possibly damaging	0.76
R6704:Mical3	UTSW	6	120,986,761 (GRCm39)	intron	probably benign
R6783:Mical3	UTSW	6	120,935,786 (GRCm39)	missense	possibly damaging	0.85
R6925:Mical3	UTSW	6	120,936,351 (GRCm39)	missense	probably benign	0.05
R6960:Mical3	UTSW	6	120,935,504 (GRCm39)	missense	probably damaging	1.00
R7170:Mical3	UTSW	6	120,950,694 (GRCm39)	splice site	probably null
R7344:Mical3	UTSW	6	121,013,505 (GRCm39)	nonsense	probably null
R7414:Mical3	UTSW	6	121,011,074 (GRCm39)	missense	probably damaging	1.00
R7455:Mical3	UTSW	6	120,935,705 (GRCm39)	missense	probably damaging	1.00
R8236:Mical3	UTSW	6	120,989,504 (GRCm39)	missense
R8286:Mical3	UTSW	6	120,998,149 (GRCm39)	missense	possibly damaging	0.68
R8316:Mical3	UTSW	6	120,911,944 (GRCm39)	missense	probably damaging	1.00
R8328:Mical3	UTSW	6	120,912,138 (GRCm39)	missense	probably damaging	0.98
R8354:Mical3	UTSW	6	120,950,381 (GRCm39)	missense	probably damaging	0.99
R8511:Mical3	UTSW	6	121,015,513 (GRCm39)	missense	possibly damaging	0.78
R8687:Mical3	UTSW	6	120,936,438 (GRCm39)	missense	probably benign	0.19
R8728:Mical3	UTSW	6	120,950,514 (GRCm39)	missense	probably damaging	0.99
R8925:Mical3	UTSW	6	120,984,325 (GRCm39)	missense	probably benign	0.00
R8927:Mical3	UTSW	6	120,984,325 (GRCm39)	missense	probably benign	0.00
R8986:Mical3	UTSW	6	120,991,822 (GRCm39)	missense
R9026:Mical3	UTSW	6	120,986,848 (GRCm39)	splice site	probably benign
R9415:Mical3	UTSW	6	120,934,712 (GRCm39)	missense	probably damaging	1.00
R9515:Mical3	UTSW	6	121,001,758 (GRCm39)	missense	probably damaging	1.00
R9720:Mical3	UTSW	6	120,935,238 (GRCm39)	missense	probably damaging	0.99
R9777:Mical3	UTSW	6	120,959,529 (GRCm39)	missense	possibly damaging	0.91
U24488:Mical3	UTSW	6	120,978,457 (GRCm39)	missense	possibly damaging	0.90
Z1177:Mical3	UTSW	6	120,936,689 (GRCm39)	missense	possibly damaging	0.71
Z1190:Mical3	UTSW	6	120,998,319 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- GAAGCCTTTGCATAGCATGG -3'
(R):5'- CCGAGATTTAGGGAGCAACTGC -3'

Sequencing Primer
(F):5'- TTTGCATAGCATGGCGGCC -3'
(R):5'- TGCCAGTGCTCGGGGTG -3'

Posted On

2019-10-24