Incidental Mutation 'R7652:Dnajc25'
ID590897
Institutional Source Beutler Lab
Gene Symbol Dnajc25
Ensembl Gene ENSMUSG00000070972
Gene NameDnaJ heat shock protein family (Hsp40) member C25
Synonyms2010203O07Rik, 2010109C08Rik
Accession Numbers
Is this an essential gene? Possibly essential (E-score: 0.655) question?
Stock #R7652 (G1)
Quality Score225.009
Status Not validated
Chromosome4
Chromosomal Location58995062-59025573 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 59020483 bp
ZygosityHeterozygous
Amino Acid Change Lysine to Arginine at position 302 (K302R)
Ref Sequence ENSEMBL: ENSMUSP00000092680 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000095070] [ENSMUST00000148366] [ENSMUST00000150309] [ENSMUST00000152199] [ENSMUST00000153467] [ENSMUST00000174664]
Predicted Effect probably benign
Transcript: ENSMUST00000095070
AA Change: K302R

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)
SMART Domains Protein: ENSMUSP00000092680
Gene: ENSMUSG00000070972
AA Change: K302R

DomainStartEndE-ValueType
signal peptide 1 37 N/A INTRINSIC
DnaJ 47 113 2.04e-19 SMART
low complexity region 125 138 N/A INTRINSIC
transmembrane domain 147 164 N/A INTRINSIC
coiled coil region 195 220 N/A INTRINSIC
transmembrane domain 241 263 N/A INTRINSIC
low complexity region 313 331 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000148366
SMART Domains Protein: ENSMUSP00000116171
Gene: ENSMUSG00000070972

DomainStartEndE-ValueType
low complexity region 6 19 N/A INTRINSIC
transmembrane domain 24 46 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000150309
SMART Domains Protein: ENSMUSP00000123172
Gene: ENSMUSG00000070972

DomainStartEndE-ValueType
signal peptide 1 37 N/A INTRINSIC
DnaJ 47 113 4.18e-16 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000152199
AA Change: K183R

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)
SMART Domains Protein: ENSMUSP00000118048
Gene: ENSMUSG00000070972
AA Change: K183R

DomainStartEndE-ValueType
low complexity region 6 19 N/A INTRINSIC
transmembrane domain 28 45 N/A INTRINSIC
coiled coil region 76 101 N/A INTRINSIC
transmembrane domain 122 144 N/A INTRINSIC
low complexity region 194 212 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000153467
SMART Domains Protein: ENSMUSP00000114320
Gene: ENSMUSG00000070972

DomainStartEndE-ValueType
signal peptide 1 37 N/A INTRINSIC
DnaJ 47 113 1.12e-16 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000174664
SMART Domains Protein: ENSMUSP00000134530
Gene: ENSMUSG00000092345

DomainStartEndE-ValueType
signal peptide 1 37 N/A INTRINSIC
DnaJ 47 113 3.9e-16 SMART
G_gamma 94 150 1.06e-6 SMART
GGL 94 150 1.05e-9 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.7%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 69 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Agrn A G 4: 156,169,218 probably null Het
Alb G C 5: 90,467,355 R242P probably damaging Het
Aph1b T C 9: 66,784,541 T195A probably benign Het
Apoe G T 7: 19,696,610 R236S possibly damaging Het
Arap3 G T 18: 37,978,452 T1137K probably damaging Het
Arhgap45 C T 10: 80,028,838 A908V probably benign Het
Atp13a1 T C 8: 69,805,559 C965R probably damaging Het
C7 A G 15: 5,012,105 Y440H probably damaging Het
Cacna2d2 T C 9: 107,524,198 probably null Het
Ddah2 C A 17: 35,061,050 R173S possibly damaging Het
Dnajc11 T C 4: 151,974,225 Y337H probably damaging Het
Dnajc6 A T 4: 101,606,677 Q209L probably damaging Het
Drosha G A 15: 12,859,436 V577I probably benign Het
E430018J23Rik C T 7: 127,393,324 C38Y probably null Het
Ebf2 G T 14: 67,390,567 probably null Het
Epx A G 11: 87,875,334 probably null Het
Eqtn T C 4: 94,928,337 Y73C probably damaging Het
Fam107b A G 2: 3,772,847 N21S probably benign Het
Fasn G A 11: 120,816,328 S857F probably damaging Het
Fat1 A G 8: 44,953,299 N1029S probably damaging Het
Gas8 A G 8: 123,526,536 I208V probably benign Het
Gm11127 A T 17: 36,056,783 V221D probably damaging Het
Gm3269 T C 14: 4,839,095 I88T probably benign Het
Gm36079 A T 13: 120,026,993 S7T possibly damaging Het
Gm8005 C A 14: 42,436,962 L136F Het
Grid2ip C A 5: 143,382,638 P743Q probably damaging Het
Hbs1l C T 10: 21,364,760 T626I probably benign Het
Hdac3 T A 18: 37,954,919 probably benign Het
Hnrnpab T C 11: 51,605,573 Y94C probably damaging Het
Homer2 T C 7: 81,649,666 D17G probably damaging Het
Ikbke C T 1: 131,271,832 R308Q probably damaging Het
Ints10 A G 8: 68,825,119 T682A possibly damaging Het
Klhl3 G A 13: 58,113,332 probably benign Het
Klk9 A T 7: 43,796,090 T235S probably benign Het
Kmt2c T C 5: 25,315,719 T1798A probably benign Het
Kri1 TTCCTCCTCCTCCTCCTCCTCCTCCTCCTC TTCCTCCTCCTCCTCCTCCTCCTCCTC 9: 21,281,056 probably benign Het
Krtap4-6 T C 11: 99,665,614 I96V unknown Het
Lef1 A C 3: 131,200,354 R354S probably damaging Het
Lrp6 A T 6: 134,511,245 L296* probably null Het
Maml2 T C 9: 13,621,649 Y720H Het
Mmrn1 A G 6: 60,977,506 N924D probably benign Het
Myh7b A G 2: 155,632,236 K1624E probably damaging Het
Nos3 T A 5: 24,383,612 V1112D probably damaging Het
Olfr1136 A G 2: 87,693,360 I174T probably damaging Het
Olfr1216 C T 2: 89,013,549 V172I probably benign Het
Olfr1384 T C 11: 49,513,685 F16L probably damaging Het
Olfr1437 C A 19: 12,322,287 C180F probably damaging Het
Olfr814 A T 10: 129,874,477 N93K probably damaging Het
Phospho1 T C 11: 95,830,819 L105S probably damaging Het
Prss35 T A 9: 86,755,970 N264K probably benign Het
Ptpn13 A G 5: 103,529,712 D732G probably benign Het
Rab18 A T 18: 6,783,123 T64S possibly damaging Het
Rft1 A G 14: 30,677,816 Q319R probably benign Het
Rgs7 T A 1: 175,093,830 M220L probably benign Het
Rnf13 A G 3: 57,764,351 N28S probably benign Het
Setd3 A T 12: 108,112,289 I311N probably damaging Het
Setd5 G A 6: 113,121,764 R786H probably damaging Het
Slc26a6 T C 9: 108,855,944 probably null Het
Slc26a9 A T 1: 131,763,896 T626S probably benign Het
Stom A T 2: 35,316,029 N229K probably benign Het
Tlr1 A T 5: 64,926,787 L149* probably null Het
Trpc3 C A 3: 36,638,528 V772F probably benign Het
Ube3a A G 7: 59,243,354 probably benign Het
Ugt2b35 T A 5: 87,001,510 F207I probably damaging Het
Usp53 A T 3: 122,953,235 D360E possibly damaging Het
Vmn2r96 T G 17: 18,573,570 S59A probably benign Het
Wdr70 A G 15: 8,079,216 C149R probably benign Het
Wnt3 A G 11: 103,812,464 T258A possibly damaging Het
Zfp729b T C 13: 67,591,252 T965A probably benign Het
Other mutations in Dnajc25
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02102:Dnajc25 APN 4 59017693 nonsense probably null
R0505:Dnajc25 UTSW 4 59020438 missense possibly damaging 0.87
R1195:Dnajc25 UTSW 4 59003415 missense probably damaging 1.00
R2437:Dnajc25 UTSW 4 59020234 missense probably damaging 1.00
R5689:Dnajc25 UTSW 4 59017716 missense probably damaging 0.99
R6023:Dnajc25 UTSW 4 59013752 missense possibly damaging 0.53
R6329:Dnajc25 UTSW 4 59013678 missense probably benign 0.33
R7316:Dnajc25 UTSW 4 59017693 nonsense probably null
R7398:Dnajc25 UTSW 4 59017824 critical splice donor site probably null
R7667:Dnajc25 UTSW 4 59020356 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GGACCTCCTCTTGTTTCAGG -3'
(R):5'- GGCACTGCACTACAATCTGAC -3'

Sequencing Primer
(F):5'- AGGTCATCCTCGCCCCTG -3'
(R):5'- TGCACTACAATCTGACAGATTACTC -3'
Posted On2019-10-24