Mutagenetix > Incidental Mutation

Incidental Mutation 'R7658:Gigyf2'

591313

Institutional Source

Beutler Lab

Gene Symbol

Gigyf2

Ensembl Gene

ENSMUSG00000048000

Gene Name

GRB10 interacting GYF protein 2

Synonyms

2610016F01Rik, Tnrc15, A830080H02Rik

MMRRC Submission

045703-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.939) question?

Stock #

R7658 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

87254720-87378518 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 87346860 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Phenylalanine at position 620 (L620F)

Ref Sequence

ENSEMBL: ENSMUSP00000027475 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027475] [ENSMUST00000164992] [ENSMUST00000172794] [ENSMUST00000172964] [ENSMUST00000173173] [ENSMUST00000174501]

AlphaFold

Q6Y7W8

Predicted Effect

unknown
Transcript: ENSMUST00000027475
AA Change: L620F

SMART Domains

Protein: ENSMUSP00000027475
Gene: ENSMUSG00000048000
AA Change: L620F

Domain	Start	End	E-Value	Type
low complexity region	66	83	N/A	INTRINSIC
low complexity region	99	132	N/A	INTRINSIC
low complexity region	143	155	N/A	INTRINSIC
low complexity region	247	285	N/A	INTRINSIC
low complexity region	330	341	N/A	INTRINSIC
internal_repeat_1	344	384	2.48e-5	PROSPERO
internal_repeat_1	404	440	2.48e-5	PROSPERO
GYF	535	590	2.83e-26	SMART
low complexity region	620	667	N/A	INTRINSIC
coiled coil region	723	1037	N/A	INTRINSIC
low complexity region	1096	1110	N/A	INTRINSIC
low complexity region	1119	1130	N/A	INTRINSIC
coiled coil region	1194	1223	N/A	INTRINSIC
low complexity region	1236	1246	N/A	INTRINSIC
low complexity region	1254	1260	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000164992
AA Change: L563F

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000129046
Gene: ENSMUSG00000048000
AA Change: L563F

Domain	Start	End	E-Value	Type
low complexity region	66	83	N/A	INTRINSIC
low complexity region	99	129	N/A	INTRINSIC
low complexity region	190	228	N/A	INTRINSIC
low complexity region	273	284	N/A	INTRINSIC
GYF	478	533	2.83e-26	SMART
low complexity region	563	610	N/A	INTRINSIC
coiled coil region	666	721	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000172794
AA Change: L614F

SMART Domains

Protein: ENSMUSP00000134077
Gene: ENSMUSG00000048000
AA Change: L614F

Domain	Start	End	E-Value	Type
low complexity region	66	83	N/A	INTRINSIC
low complexity region	99	132	N/A	INTRINSIC
low complexity region	143	155	N/A	INTRINSIC
low complexity region	241	279	N/A	INTRINSIC
low complexity region	324	335	N/A	INTRINSIC
internal_repeat_1	338	378	2.29e-5	PROSPERO
internal_repeat_1	398	434	2.29e-5	PROSPERO
GYF	529	584	2.83e-26	SMART
low complexity region	614	661	N/A	INTRINSIC
coiled coil region	717	1031	N/A	INTRINSIC
low complexity region	1090	1104	N/A	INTRINSIC
low complexity region	1113	1124	N/A	INTRINSIC
coiled coil region	1188	1217	N/A	INTRINSIC
low complexity region	1230	1240	N/A	INTRINSIC
low complexity region	1248	1254	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000172964
AA Change: L620F

SMART Domains

Protein: ENSMUSP00000133392
Gene: ENSMUSG00000048000
AA Change: L620F

Domain	Start	End	E-Value	Type
low complexity region	66	83	N/A	INTRINSIC
low complexity region	99	132	N/A	INTRINSIC
low complexity region	143	155	N/A	INTRINSIC
low complexity region	247	285	N/A	INTRINSIC
low complexity region	330	341	N/A	INTRINSIC
internal_repeat_1	344	384	3.03e-5	PROSPERO
internal_repeat_1	404	440	3.03e-5	PROSPERO
GYF	535	590	2.83e-26	SMART
low complexity region	620	667	N/A	INTRINSIC
SCOP:d1eq1a_	724	859	1e-2	SMART
low complexity region	953	972	N/A	INTRINSIC
low complexity region	1008	1031	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000173173
AA Change: L613F

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000134193
Gene: ENSMUSG00000048000
AA Change: L613F

Domain	Start	End	E-Value	Type
low complexity region	66	83	N/A	INTRINSIC
low complexity region	99	132	N/A	INTRINSIC
low complexity region	143	155	N/A	INTRINSIC
low complexity region	241	279	N/A	INTRINSIC
low complexity region	324	335	N/A	INTRINSIC
GYF	528	583	2.83e-26	SMART
low complexity region	613	660	N/A	INTRINSIC
SCOP:d1eq1a_	717	852	1e-3	SMART

Predicted Effect

SMART Domains

Protein: ENSMUSP00000134677
Gene: ENSMUSG00000048000
AA Change: L441F

Domain	Start	End	E-Value	Type
low complexity region	69	107	N/A	INTRINSIC
low complexity region	152	163	N/A	INTRINSIC
internal_repeat_1	166	206	3.2e-5	PROSPERO
internal_repeat_1	226	262	3.2e-5	PROSPERO
GYF	357	412	2.83e-26	SMART
low complexity region	442	489	N/A	INTRINSIC
coiled coil region	544	745	N/A	INTRINSIC
low complexity region	775	786	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000173636
AA Change: I225L

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

Predicted Effect

unknown
Transcript: ENSMUST00000174501
AA Change: L620F

SMART Domains

Protein: ENSMUSP00000133327
Gene: ENSMUSG00000048000
AA Change: L620F

Domain	Start	End	E-Value	Type
low complexity region	66	83	N/A	INTRINSIC
low complexity region	99	132	N/A	INTRINSIC
low complexity region	143	155	N/A	INTRINSIC
low complexity region	247	285	N/A	INTRINSIC
low complexity region	330	341	N/A	INTRINSIC
internal_repeat_1	344	384	2.48e-5	PROSPERO
internal_repeat_1	404	440	2.48e-5	PROSPERO
GYF	535	590	2.83e-26	SMART
low complexity region	620	667	N/A	INTRINSIC
coiled coil region	723	1037	N/A	INTRINSIC
low complexity region	1096	1110	N/A	INTRINSIC
low complexity region	1119	1130	N/A	INTRINSIC
coiled coil region	1194	1223	N/A	INTRINSIC
low complexity region	1236	1246	N/A	INTRINSIC
low complexity region	1254	1260	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene contains CAG trinucleotide repeats and encodes a protein containing several stretches of polyglutamine residues. The encoded protein may be involved in the regulation of tyrosine kinase receptor signaling. This gene is located in a chromosomal region that was genetically linked to Parkinson disease type 11, and mutations in this gene were thought to be causative for this disease. However, more recent studies in different populations have been unable to replicate this association. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit neonatal and postnatal lethality. Mice heterozygous for a knock-out allele exhibit impaired motor coordination with motor neuron degeneration. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 95 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110025L11Rik	G	T	16: 88,860,618 (GRCm39)	S72Y	unknown	Het
Abca8b	T	A	11: 109,826,543 (GRCm39)	K1568N	probably benign	Het
Adcy6	T	A	15: 98,493,948 (GRCm39)	Y865F	probably benign	Het
Adgrf3	A	T	5: 30,402,204 (GRCm39)	V608D	probably benign	Het
Agbl1	G	T	7: 76,416,117 (GRCm39)	A965S	unknown	Het
Agbl3	C	T	6: 34,809,443 (GRCm39)	P690L	probably benign	Het
Akap9	C	G	5: 4,018,745 (GRCm39)	H1109D	probably benign	Het
Amt	A	C	9: 108,174,430 (GRCm39)	H65P	probably damaging	Het
Ankrd11	T	A	8: 123,620,403 (GRCm39)	T1150S	probably benign	Het
Arhgap15	A	C	2: 44,032,280 (GRCm39)	H288P	probably benign	Het
Arhgap39	A	T	15: 76,621,617 (GRCm39)	M328K	probably benign	Het
Arhgef12	T	C	9: 42,903,832 (GRCm39)	K743R	probably damaging	Het
Atg2a	T	G	19: 6,301,293 (GRCm39)	V789G	probably damaging	Het
Ccdc54	T	C	16: 50,410,844 (GRCm39)	T141A	probably benign	Het
Ccng2	C	G	5: 93,421,202 (GRCm39)	S237R	probably benign	Het
Cdh5	T	C	8: 104,856,033 (GRCm39)		probably null	Het
Cdkl3	T	C	11: 51,918,009 (GRCm39)	V404A	not run	Het
Chrm2	A	T	6: 36,500,184 (GRCm39)	I14F	probably benign	Het
Cnbp	T	C	6: 87,822,258 (GRCm39)	K89E	possibly damaging	Het
Cntn6	T	A	6: 104,627,444 (GRCm39)	D92E	probably benign	Het
Col15a1	T	C	4: 47,245,591 (GRCm39)	F114S	possibly damaging	Het
Csrnp1	A	G	9: 119,801,469 (GRCm39)	F530S	probably benign	Het
Dcun1d3	C	T	7: 119,456,891 (GRCm39)	V274M	probably damaging	Het
Dph2	T	A	4: 117,747,478 (GRCm39)	H302L	possibly damaging	Het
Fam162a	A	T	16: 35,866,770 (GRCm39)	Y118*	probably null	Het
Fam186a	A	G	15: 99,837,725 (GRCm39)	Y2840H	unknown	Het
Fto	A	G	8: 92,392,950 (GRCm39)	K466E	probably benign	Het
Gal	T	A	19: 3,463,309 (GRCm39)	Y41F	probably damaging	Het
Git2	C	T	5: 114,904,550 (GRCm39)	R123H	probably damaging	Het
Glud1	A	T	14: 34,033,114 (GRCm39)	E87V	probably benign	Het
Gm21190	T	G	5: 15,732,923 (GRCm39)	E94A	possibly damaging	Het
Gm5592	T	C	7: 40,938,134 (GRCm39)	V472A	probably benign	Het
Gpc5	T	A	14: 115,665,620 (GRCm39)	N481K	possibly damaging	Het
Gpn2	A	G	4: 133,318,687 (GRCm39)	E304G	probably benign	Het
Gsdmc2	T	C	15: 63,696,903 (GRCm39)	T423A	probably damaging	Het
Gucy2e	G	A	11: 69,117,055 (GRCm39)	Q789*	probably null	Het
Gxylt2	T	A	6: 100,760,104 (GRCm39)	V213E	probably damaging	Het
Ighv1-75	G	A	12: 115,797,731 (GRCm39)	L64F	possibly damaging	Het
Il17re	T	C	6: 113,435,943 (GRCm39)	C30R	probably benign	Het
Il7	G	T	3: 7,669,142 (GRCm39)	D31E	probably benign	Het
Ints4	T	C	7: 97,178,460 (GRCm39)	Y687H	possibly damaging	Het
Kdelr1	T	G	7: 45,532,401 (GRCm39)	V202G	probably benign	Het
Khnyn	C	A	14: 56,124,596 (GRCm39)	Y283*	probably null	Het
Klf11	T	C	12: 24,703,670 (GRCm39)	V52A	probably damaging	Het
Klhl7	A	G	5: 24,346,284 (GRCm39)	N310S	probably benign	Het
Krt27	A	T	11: 99,240,312 (GRCm39)	L202Q	possibly damaging	Het
Lce1d	A	G	3: 92,593,354 (GRCm39)	C20R	unknown	Het
Lim2	A	T	7: 43,083,054 (GRCm39)	I80F	possibly damaging	Het
Lrrk2	T	A	15: 91,584,561 (GRCm39)	F326Y	possibly damaging	Het
Lyst	A	T	13: 13,905,061 (GRCm39)	Y3246F	possibly damaging	Het
Mafb	T	A	2: 160,208,355 (GRCm39)	H81L	possibly damaging	Het
Mfsd5	G	A	15: 102,189,312 (GRCm39)	R228H	probably benign	Het
Mmp1b	A	T	9: 7,386,675 (GRCm39)	F150I	possibly damaging	Het
Mthfd1	T	A	12: 76,317,209 (GRCm39)	L20Q	probably damaging	Het
Mxra8	A	G	4: 155,927,420 (GRCm39)	T402A	probably benign	Het
Ndc80	A	T	17: 71,815,658 (GRCm39)	L376M	probably damaging	Het
Nsd1	A	T	13: 55,425,452 (GRCm39)	R1536S	probably damaging	Het
Nup210l	C	A	3: 90,119,300 (GRCm39)	H1874Q	probably benign	Het
Nyap1	T	C	5: 137,731,236 (GRCm39)	H776R	probably benign	Het
Patj	C	A	4: 98,576,416 (GRCm39)	H1773Q	probably damaging	Het
Pax8	T	A	2: 24,326,523 (GRCm39)	T280S	probably benign	Het
Pcdhb19	A	G	18: 37,632,034 (GRCm39)	T610A	probably damaging	Het
Pde2a	A	G	7: 101,160,788 (GRCm39)	D919G	possibly damaging	Het
Pdk2	T	A	11: 94,919,791 (GRCm39)	Y240F	probably damaging	Het
Peg3	A	T	7: 6,712,609 (GRCm39)	I871N	probably damaging	Het
Pex1	A	T	5: 3,646,244 (GRCm39)		probably benign	Het
Pgm2l1	C	T	7: 99,899,535 (GRCm39)	R50W	probably damaging	Het
Phkg1	T	A	5: 129,894,764 (GRCm39)	K262N	probably damaging	Het
Pik3r4	A	G	9: 105,521,710 (GRCm39)	E92G	probably damaging	Het
Prmt6	A	T	3: 110,157,701 (GRCm39)	V196E	possibly damaging	Het
Ptprn2	T	G	12: 116,685,739 (GRCm39)	M66R	probably benign	Het
Rai14	C	T	15: 10,593,189 (GRCm39)	G152R	probably damaging	Het
Recql5	A	T	11: 115,814,102 (GRCm39)	S348T	probably damaging	Het
Rfk	T	G	19: 17,376,046 (GRCm39)		probably null	Het
Ro60	T	C	1: 143,646,611 (GRCm39)	T45A	probably damaging	Het
Selenbp1	A	T	3: 94,851,413 (GRCm39)	M389L	probably benign	Het
Sipa1l2	G	A	8: 126,219,029 (GRCm39)	R103C	probably benign	Het
Slc10a6	T	A	5: 103,777,056 (GRCm39)	S15C	probably damaging	Het
Slc12a2	G	T	18: 58,065,596 (GRCm39)	V944L	probably benign	Het
Slc16a11	T	A	11: 70,106,143 (GRCm39)	L127Q	possibly damaging	Het
Spef1l	T	C	7: 139,556,531 (GRCm39)	N152S	probably benign	Het
St6gal1	A	G	16: 23,174,978 (GRCm39)	Y272C	probably damaging	Het
Stab2	C	A	10: 86,816,999 (GRCm39)	V133F	probably benign	Het
Stradb	G	A	1: 59,031,885 (GRCm39)	V266I	probably damaging	Het
Tmem150b	A	G	7: 4,723,758 (GRCm39)	W140R	probably benign	Het
Tnnt3	A	T	7: 142,065,833 (GRCm39)	K157*	probably null	Het
Ttn	T	G	2: 76,554,113 (GRCm39)	K30863N	probably damaging	Het
Vldlr	G	A	19: 27,220,536 (GRCm39)	R592H	probably benign	Het
Vmn1r12	A	T	6: 57,135,883 (GRCm39)		probably benign	Het
Vmn2r63	A	C	7: 42,574,693 (GRCm39)	S519R	probably damaging	Het
Zbtb22	G	C	17: 34,137,471 (GRCm39)	E539Q	probably damaging	Het
Zbtb44	G	A	9: 30,965,375 (GRCm39)	A262T	probably benign	Het
Zfp280d	A	T	9: 72,231,354 (GRCm39)	N455I	probably damaging	Het
Zfp287	T	C	11: 62,616,089 (GRCm39)	N201D	probably damaging	Het
Zfp988	T	C	4: 147,416,751 (GRCm39)	L395P	probably damaging	Het

Other mutations in Gigyf2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00490:Gigyf2	APN	1	87,364,572 (GRCm39)	missense	probably damaging	0.99
IGL01828:Gigyf2	APN	1	87,346,820 (GRCm39)	missense	probably damaging	1.00
IGL02222:Gigyf2	APN	1	87,338,585 (GRCm39)	splice site	probably null
IGL02259:Gigyf2	APN	1	87,339,559 (GRCm39)	missense	probably damaging	1.00
IGL02562:Gigyf2	APN	1	87,335,097 (GRCm39)	missense	probably benign	0.15
IGL02565:Gigyf2	APN	1	87,369,858 (GRCm39)	missense	probably damaging	1.00
IGL02695:Gigyf2	APN	1	87,344,549 (GRCm39)	missense	probably benign	0.07
IGL03264:Gigyf2	APN	1	87,376,790 (GRCm39)	splice site	probably benign
Flop	UTSW	1	87,292,988 (GRCm39)	missense	probably damaging	1.00
FR4449:Gigyf2	UTSW	1	87,356,307 (GRCm39)	unclassified	probably benign
PIT4260001:Gigyf2	UTSW	1	87,346,828 (GRCm39)	missense	unknown
R0041:Gigyf2	UTSW	1	87,306,698 (GRCm39)	missense	probably damaging	1.00
R0126:Gigyf2	UTSW	1	87,339,597 (GRCm39)	splice site	probably benign
R0190:Gigyf2	UTSW	1	87,356,410 (GRCm39)	unclassified	probably benign
R0244:Gigyf2	UTSW	1	87,306,737 (GRCm39)	missense	possibly damaging	0.96
R0492:Gigyf2	UTSW	1	87,368,568 (GRCm39)	missense	probably damaging	1.00
R0526:Gigyf2	UTSW	1	87,349,215 (GRCm39)	missense	probably benign	0.00
R0612:Gigyf2	UTSW	1	87,376,802 (GRCm39)	missense	probably damaging	1.00
R0731:Gigyf2	UTSW	1	87,335,449 (GRCm39)	splice site	probably benign
R0783:Gigyf2	UTSW	1	87,334,883 (GRCm39)	missense	probably damaging	0.99
R1445:Gigyf2	UTSW	1	87,371,360 (GRCm39)	splice site	probably benign
R1620:Gigyf2	UTSW	1	87,376,850 (GRCm39)	missense	probably damaging	1.00
R1678:Gigyf2	UTSW	1	87,344,705 (GRCm39)	missense	probably benign	0.44
R2008:Gigyf2	UTSW	1	87,301,835 (GRCm39)	critical splice donor site	probably null
R2111:Gigyf2	UTSW	1	87,368,452 (GRCm39)	missense	probably damaging	0.99
R2112:Gigyf2	UTSW	1	87,368,452 (GRCm39)	missense	probably damaging	0.99
R2180:Gigyf2	UTSW	1	87,344,642 (GRCm39)	missense	probably damaging	1.00
R3438:Gigyf2	UTSW	1	87,368,302 (GRCm39)	missense	probably damaging	0.96
R3690:Gigyf2	UTSW	1	87,349,238 (GRCm39)	missense	possibly damaging	0.80
R4089:Gigyf2	UTSW	1	87,371,394 (GRCm39)	missense	probably damaging	1.00
R4411:Gigyf2	UTSW	1	87,364,582 (GRCm39)	missense	probably damaging	1.00
R4412:Gigyf2	UTSW	1	87,364,582 (GRCm39)	missense	probably damaging	1.00
R4489:Gigyf2	UTSW	1	87,368,548 (GRCm39)	missense	probably damaging	1.00
R4743:Gigyf2	UTSW	1	87,292,970 (GRCm39)	nonsense	probably null
R4769:Gigyf2	UTSW	1	87,368,571 (GRCm39)	missense	probably damaging	1.00
R4854:Gigyf2	UTSW	1	87,282,135 (GRCm39)	unclassified	probably benign
R5215:Gigyf2	UTSW	1	87,292,988 (GRCm39)	missense	probably damaging	1.00
R5326:Gigyf2	UTSW	1	87,352,860 (GRCm39)	unclassified	probably benign
R5771:Gigyf2	UTSW	1	87,374,050 (GRCm39)	missense	possibly damaging	0.90
R5813:Gigyf2	UTSW	1	87,368,485 (GRCm39)	missense	probably damaging	0.99
R5964:Gigyf2	UTSW	1	87,334,889 (GRCm39)	missense	probably damaging	1.00
R6026:Gigyf2	UTSW	1	87,368,454 (GRCm39)	missense	probably damaging	0.99
R6035:Gigyf2	UTSW	1	87,338,450 (GRCm39)	missense	possibly damaging	0.93
R6035:Gigyf2	UTSW	1	87,338,450 (GRCm39)	missense	possibly damaging	0.93
R6784:Gigyf2	UTSW	1	87,371,396 (GRCm39)	missense	probably damaging	1.00
R6800:Gigyf2	UTSW	1	87,346,898 (GRCm39)	missense	possibly damaging	0.68
R6991:Gigyf2	UTSW	1	87,334,858 (GRCm39)	missense	probably damaging	1.00
R7224:Gigyf2	UTSW	1	87,331,447 (GRCm39)	missense	unknown
R7464:Gigyf2	UTSW	1	87,356,326 (GRCm39)	missense	unknown
R7554:Gigyf2	UTSW	1	87,335,292 (GRCm39)	missense	unknown
R7976:Gigyf2	UTSW	1	87,331,458 (GRCm39)	missense	unknown
R8032:Gigyf2	UTSW	1	87,334,735 (GRCm39)	missense	unknown
R8070:Gigyf2	UTSW	1	87,368,629 (GRCm39)	missense	probably benign	0.03
R8071:Gigyf2	UTSW	1	87,374,155 (GRCm39)	missense	probably damaging	0.99
R8519:Gigyf2	UTSW	1	87,338,431 (GRCm39)	missense	probably benign	0.01
R8675:Gigyf2	UTSW	1	87,331,438 (GRCm39)	missense	unknown
R8849:Gigyf2	UTSW	1	87,361,592 (GRCm39)	missense	unknown
R8872:Gigyf2	UTSW	1	87,307,725 (GRCm39)	missense	unknown
R9184:Gigyf2	UTSW	1	87,368,311 (GRCm39)	missense	possibly damaging	0.95
R9465:Gigyf2	UTSW	1	87,334,775 (GRCm39)	missense	unknown
R9502:Gigyf2	UTSW	1	87,331,446 (GRCm39)	missense	unknown
R9616:Gigyf2	UTSW	1	87,356,326 (GRCm39)	missense	unknown
R9665:Gigyf2	UTSW	1	87,331,457 (GRCm39)	missense	unknown
X0065:Gigyf2	UTSW	1	87,339,589 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CACTAGGTGGCATTGCTTGG -3'
(R):5'- ACGTCACTAGTAGGCTACTCAAG -3'

Sequencing Primer
(F):5'- TTTGAAGGGCACAAGTTTGGAG -3'
(R):5'- GAATCACAGAAGCCTTGAGTTCTC -3'

Posted On

2019-11-12