Mutagenetix > Incidental Mutations

Incidental Mutation 'R7659:Olfr847'

591444

Institutional Source

Beutler Lab

Gene Symbol

Olfr847

Ensembl Gene

ENSMUSG00000059821

Gene Name

olfactory receptor 847

Synonyms

MOR149-2, GA_x6K02T2PVTD-13113073-13112135

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.140) question?

Stock #

R7659 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19373668-19378669 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 19375558 bp

Zygosity

Heterozygous

Amino Acid Change

Alanine to Threonine at position 108 (A108T)

Ref Sequence

ENSEMBL: ENSMUSP00000151112 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079620] [ENSMUST00000216839]

Predicted Effect

probably benign
Transcript: ENSMUST00000079620
AA Change: A108T

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000078568
Gene: ENSMUSG00000059821
AA Change: A108T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.9e-56	PFAM
Pfam:7tm_1	41	290	2.1e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000216839
AA Change: A108T

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

100% (66/66)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aldh2	T	C	5: 121,568,960	E498G	probably damaging	Het
Ankar	T	C	1: 72,690,135	T284A	possibly damaging	Het
Arid5b	C	T	10: 68,098,587	G495E	probably benign	Het
Armh1	C	A	4: 117,213,741	A396S	probably benign	Het
Atp6v0a4	A	G	6: 38,071,972	I452T	probably damaging	Het
Azgp1	G	T	5: 137,987,513	A133S	probably damaging	Het
Bmp2k	T	C	5: 97,074,719	S667P	unknown	Het
C1qtnf9	T	A	14: 60,772,304	I3N	possibly damaging	Het
C530025M09Rik	T	G	2: 149,830,619	T199P	unknown	Het
Casc3	C	G	11: 98,809,873	R4G	unknown	Het
Cass4	A	G	2: 172,427,027	D343G	probably damaging	Het
Ccdc84	T	C	9: 44,413,493	E164G	probably damaging	Het
Col6a3	T	A	1: 90,781,745	T2517S	unknown	Het
Dbr1	A	G	9: 99,576,610	T78A	probably damaging	Het
Dnah9	A	G	11: 65,989,780	F2664L	probably damaging	Het
Eif2ak1	T	C	5: 143,889,462	I431T	probably damaging	Het
Got1	A	G	19: 43,504,639	V281A	probably benign	Het
Gpld1	A	C	13: 24,979,981	R555S	probably benign	Het
Gpr149	T	C	3: 62,603,835	R248G	probably benign	Het
Hps3	G	A	3: 20,022,814	R395*	probably null	Het
Inpp5b	T	C	4: 124,795,426	L833P	probably damaging	Het
Itgb4	A	T	11: 115,979,731	S129C	probably damaging	Het
Kif1a	T	A	1: 93,046,820		probably benign	Het
Lgr4	T	A	2: 109,996,766	V205E	probably damaging	Het
Mbd3	T	C	10: 80,395,185	N96S	probably damaging	Het
Mcam	T	A	9: 44,136,770	C136S	unknown	Het
Mki67	A	G	7: 135,697,426	S1960P	probably damaging	Het
Mogs	G	A	6: 83,116,825		probably null	Het
Muc6	T	C	7: 141,637,060	T2567A	possibly damaging	Het
Mug1	C	A	6: 121,861,220	H470N	possibly damaging	Het
Nae1	A	G	8: 104,516,164	V432A	probably benign	Het
Nav3	T	G	10: 109,766,990	T1233P	probably benign	Het
Nme9	G	A	9: 99,470,857	A287T	possibly damaging	Het
Nmrk1	A	G	19: 18,636,135	I8V	probably benign	Het
Noa1	T	C	5: 77,309,390	N223D	not run	Het
Nr4a3	T	C	4: 48,051,269	Y8H	probably damaging	Het
Olfr1112	T	A	2: 87,192,365	V226E	possibly damaging	Het
Olfr1181	T	C	2: 88,423,926	Y33C	probably damaging	Het
Olfr124	A	T	17: 37,805,163	Q6L	probably benign	Het
Olfr25	T	C	9: 38,329,716	V40A	possibly damaging	Het
Olfr344	T	A	2: 36,568,625	V9E	possibly damaging	Het
Olfr649	T	C	7: 104,190,187	S7G	probably benign	Het
Olfr733	T	C	14: 50,299,241	I23V	probably benign	Het
Otoa	T	A	7: 121,134,044	H711Q	probably benign	Het
Otogl	T	A	10: 107,777,120	E2048D	probably benign	Het
Paip1	C	T	13: 119,450,770	T390I	possibly damaging	Het
Pcdha3	A	G	18: 36,948,166	T654A	probably benign	Het
Pigg	G	A	5: 108,338,619	V713I	probably benign	Het
Ppt1	T	A	4: 122,836,333	H26Q	probably benign	Het
Prim1	T	C	10: 128,026,589		probably null	Het
Prmt7	G	T	8: 106,237,286	A271S	probably benign	Het
Ptprd	T	C	4: 76,128,916	D124G	probably benign	Het
Rad54l2	T	C	9: 106,713,578	K450E	probably benign	Het
Rasgrp2	G	T	19: 6,401,827	V13L	probably damaging	Het
Sapcd2	A	G	2: 25,375,966		probably null	Het
Siglecf	A	G	7: 43,351,770	Y54C	probably damaging	Het
Stk31	T	A	6: 49,423,406	N388K	probably benign	Het
Timm23	A	C	14: 32,198,978	Y52*	probably null	Het
Tm9sf1	A	T	14: 55,636,335	F569I	probably damaging	Het
Tmprss9	C	A	10: 80,893,009	A592E	probably damaging	Het
Trim30d	A	T	7: 104,472,347	V397E	probably benign	Het
Tshz1	A	T	18: 84,016,075	N69K	probably damaging	Het
Upk2	A	C	9: 44,453,511	S130R	probably damaging	Het
Vmn1r80	A	G	7: 12,193,050	Y29C	probably damaging	Het
Vmn2r96	T	C	17: 18,573,487	I31T	probably benign	Het
Zfp975	T	C	7: 42,662,424	Q255R	probably benign	Het

Other mutations in Olfr847

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01150:Olfr847	APN	9	19375239	missense	probably damaging	1.00
IGL01293:Olfr847	APN	9	19375336	missense	probably benign	0.23
IGL01879:Olfr847	APN	9	19375407	nonsense	probably null
IGL03298:Olfr847	APN	9	19375062	missense	probably damaging	1.00
R1350:Olfr847	UTSW	9	19375414	missense	possibly damaging	0.94
R1400:Olfr847	UTSW	9	19375062	missense	probably damaging	0.98
R2894:Olfr847	UTSW	9	19375292	nonsense	probably null
R4468:Olfr847	UTSW	9	19375648	missense	probably benign	0.00
R4694:Olfr847	UTSW	9	19375398	missense	probably damaging	1.00
R4791:Olfr847	UTSW	9	19375809	missense	probably benign	0.28
R4794:Olfr847	UTSW	9	19375545	missense	probably benign	0.00
R5517:Olfr847	UTSW	9	19375767	missense	probably damaging	1.00
R5599:Olfr847	UTSW	9	19375629	missense	possibly damaging	0.77
R5777:Olfr847	UTSW	9	19375718	missense	probably benign	0.29
R6505:Olfr847	UTSW	9	19374941	makesense	probably null
R6509:Olfr847	UTSW	9	19375143	missense	probably benign
R7246:Olfr847	UTSW	9	19375465	nonsense	probably null
R7789:Olfr847	UTSW	9	19375065	missense	probably benign	0.33
R7886:Olfr847	UTSW	9	19375906	splice site	probably null
R7969:Olfr847	UTSW	9	19375906	splice site	probably null
Z1088:Olfr847	UTSW	9	19375684	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTTGTGCAAAATGACAACCGC -3'
(R):5'- GGTTACAATTGTGGGAAATTTGCTC -3'

Sequencing Primer
(F):5'- TGTGCAAAATGACAACCGCAGTATC -3'
(R):5'- GGGAAATTTGCTCATCATCATTGCTG -3'

Posted On

2019-11-12