Mutagenetix > Incidental Mutation

Incidental Mutation 'R7665:Zfp51'

591895

Institutional Source

Beutler Lab

Gene Symbol

Zfp51

Ensembl Gene

ENSMUSG00000023892

Gene Name

zinc finger protein 51

Synonyms

Zfp-51, zfec12

MMRRC Submission

045739-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.452) question?

Stock #

R7665 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

21450374-21465587 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 21463581 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 153 (T153A)

Ref Sequence

ENSEMBL: ENSMUSP00000045684 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039577]

AlphaFold

Q3U4L8

Predicted Effect

probably benign
Transcript: ENSMUST00000039577
AA Change: T153A

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000045684
Gene: ENSMUSG00000023892
AA Change: T153A

Domain	Start	End	E-Value	Type
KRAB	43	103	1.71e-22	SMART
ZnF_C2H2	214	236	2.36e-2	SMART
ZnF_C2H2	242	264	8.94e-3	SMART
ZnF_C2H2	270	292	7.9e-4	SMART
ZnF_C2H2	298	320	3.44e-4	SMART
ZnF_C2H2	326	348	7.15e-2	SMART
ZnF_C2H2	354	376	4.54e-4	SMART
ZnF_C2H2	382	404	3.44e-4	SMART
ZnF_C2H2	410	432	9.88e-5	SMART
ZnF_C2H2	438	460	3.16e-3	SMART
ZnF_C2H2	466	488	1.58e-3	SMART
ZnF_C2H2	494	517	2.57e-3	SMART
ZnF_C2H2	523	545	3.63e-3	SMART
ZnF_C2H2	551	573	1.4e-4	SMART
ZnF_C2H2	579	601	4.17e-3	SMART
ZnF_C2H2	607	629	2.79e-4	SMART
ZnF_C2H2	635	657	4.24e-4	SMART
ZnF_C2H2	663	685	9.08e-4	SMART
ZnF_C2H2	691	713	8.47e-4	SMART
ZnF_C2H2	719	741	1.36e-2	SMART
ZnF_C2H2	747	769	6.82e1	SMART

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

100% (72/72)

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930519G04Rik	A	G	5: 114,874,323 (GRCm38)		probably null	Het
A830018L16Rik	T	C	1: 11,972,099 (GRCm38)	S448P	probably damaging	Het
Abca4	C	T	3: 122,044,490 (GRCm38)		probably benign	Het
Ackr2	A	G	9: 121,909,308 (GRCm38)	M250V	probably benign	Het
Actn4	A	G	7: 28,916,207 (GRCm38)	I147T	probably damaging	Het
Adgrv1	C	T	13: 81,499,142 (GRCm38)	S3093N	probably damaging	Het
Arid5b	C	T	10: 68,098,587 (GRCm38)	G495E	probably benign	Het
Armh1	C	A	4: 117,213,741 (GRCm38)	A396S	probably benign	Het
Brwd1	G	A	16: 96,041,343 (GRCm38)	T798M	probably benign	Het
Cdk13	G	A	13: 17,772,553 (GRCm38)	T540I	possibly damaging	Het
Cdkn1c	A	G	7: 143,460,634 (GRCm38)	V25A	possibly damaging	Het
Col2a1	T	C	15: 97,976,700 (GRCm38)	E1420G	unknown	Het
Dbf4	G	A	5: 8,397,867 (GRCm38)	P448S	probably damaging	Het
Dnajb7	T	C	15: 81,407,419 (GRCm38)	N239S	probably benign	Het
Dnttip1	A	G	2: 164,754,141 (GRCm38)	D102G	probably damaging	Het
Dpp8	T	A	9: 65,078,718 (GRCm38)	V830D	probably damaging	Het
Eddm13	G	A	7: 6,269,892 (GRCm38)		probably null	Het
Eef1g	T	C	19: 8,968,289 (GRCm38)	V29A	probably benign	Het
Enpp2	T	A	15: 54,839,394 (GRCm38)	Y906F	probably damaging	Het
Epb41l4a	A	G	18: 34,006,016 (GRCm38)	L23P	possibly damaging	Het
Exoc1	T	A	5: 76,543,573 (GRCm38)	M248K	probably benign	Het
Fam83b	T	C	9: 76,490,875 (GRCm38)	Y982C	probably damaging	Het
Fat4	C	T	3: 38,889,178 (GRCm38)	A740V	probably benign	Het
Fsip2	T	A	2: 82,981,805 (GRCm38)	S2823T	probably benign	Het
Gckr	T	C	5: 31,297,555 (GRCm38)			Het
Gpr150	A	T	13: 76,055,974 (GRCm38)	V284E	probably damaging	Het
Grtp1	T	G	8: 13,177,103 (GRCm38)	I344L	probably benign	Het
Heatr5a	T	A	12: 51,961,530 (GRCm38)	N10I	probably damaging	Het
Herc2	C	A	7: 56,153,155 (GRCm38)	L2109I	probably damaging	Het
Hs1bp3	T	A	12: 8,317,935 (GRCm38)	D61E	probably damaging	Het
Ifit1bl1	T	A	19: 34,594,883 (GRCm38)	Y58F	probably benign	Het
Itfg1	A	G	8: 85,764,350 (GRCm38)	F317L	probably benign	Het
Itsn1	T	C	16: 91,841,603 (GRCm38)	I764T	unknown	Het
Med8	A	C	4: 118,411,656 (GRCm38)		probably null	Het
Mpeg1	C	A	19: 12,463,094 (GRCm38)	P639T	probably damaging	Het
Mtcl3	T	A	10: 29,196,397 (GRCm38)	Y562N	probably damaging	Het
Nedd9	A	G	13: 41,316,309 (GRCm38)	L456P	probably benign	Het
Neo1	A	G	9: 58,925,795 (GRCm38)	S556P	probably damaging	Het
Nphp3	T	A	9: 104,005,393 (GRCm38)		probably null	Het
Nup205	T	C	6: 35,177,620 (GRCm38)	V53A	possibly damaging	Het
Nvl	A	T	1: 181,134,944 (GRCm38)	S154T	probably benign	Het
Olfr391-ps	A	T	11: 73,798,961 (GRCm38)	N265K	probably benign	Het
Or10h1	G	T	17: 33,199,629 (GRCm38)	G194*	probably null	Het
Or2ag2	A	T	7: 106,886,673 (GRCm38)	V48D	possibly damaging	Het
Or2h2c	A	G	17: 37,111,391 (GRCm38)	M197T	probably benign	Het
Or51ah3	A	T	7: 103,561,316 (GRCm38)	I280F	probably benign	Het
Or9k7	T	A	10: 130,211,261 (GRCm38)		probably null	Het
Parvg	T	C	15: 84,337,801 (GRCm38)	I243T	probably damaging	Het
Paxip1	T	A	5: 27,765,738 (GRCm38)	M538L	unknown	Het
Pgghg	G	T	7: 140,945,469 (GRCm38)	D428Y	probably damaging	Het
Pik3cd	C	T	4: 149,654,050 (GRCm38)	V777M	possibly damaging	Het
Plcl2	A	C	17: 50,607,157 (GRCm38)	K398T	probably benign	Het
Plxna1	A	T	6: 89,324,538 (GRCm38)		probably null	Het
Rbbp6	T	A	7: 122,990,032 (GRCm38)		probably null	Het
Rbbp6	T	C	7: 122,994,686 (GRCm38)	Y514H	possibly damaging	Het
Scin	T	A	12: 40,069,415 (GRCm38)	N538I	probably damaging	Het
Sdcbp	A	G	4: 6,385,144 (GRCm38)	D121G	probably benign	Het
Sgk1	T	C	10: 21,996,662 (GRCm38)	I311T	probably damaging	Het
Shq1	A	C	6: 100,573,756 (GRCm38)	L407W	probably damaging	Het
Sipa1	A	T	19: 5,651,671 (GRCm38)	S979T	probably benign	Het
Slc25a37	G	T	14: 69,249,579 (GRCm38)	T85K	probably benign	Het
Spag9	A	T	11: 94,013,654 (GRCm38)	Q112L	probably damaging	Het
Spg11	A	T	2: 122,066,267 (GRCm38)	V1686E	probably damaging	Het
Stap2	A	G	17: 55,997,909 (GRCm38)	V291A	probably benign	Het
Tnk2	C	T	16: 32,680,526 (GRCm38)	R886C	probably damaging	Het
Tnrc6c	T	A	11: 117,720,951 (GRCm38)	D138E	possibly damaging	Het
Unc13c	A	G	9: 73,680,474 (GRCm38)	S1426P	probably benign	Het
Vav1	G	A	17: 57,297,086 (GRCm38)	V163M	probably damaging	Het
Vmn2r67	T	A	7: 85,151,988 (GRCm38)	K247*	probably null	Het
Zc2hc1c	T	C	12: 85,296,562 (GRCm38)	V491A	possibly damaging	Het
Zyx	A	G	6: 42,356,162 (GRCm38)	E374G	probably damaging	Het

Other mutations in Zfp51

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00420:Zfp51	APN	17	21,463,452 (GRCm38)	missense	probably benign	0.11
IGL00971:Zfp51	APN	17	21,463,582 (GRCm38)	missense	probably benign	0.03
IGL02002:Zfp51	APN	17	21,463,959 (GRCm38)	missense	probably damaging	0.98
IGL02268:Zfp51	APN	17	21,463,419 (GRCm38)	nonsense	probably null
IGL03249:Zfp51	APN	17	21,463,439 (GRCm38)	missense	probably damaging	1.00
R1569:Zfp51	UTSW	17	21,456,380 (GRCm38)	missense	probably benign	0.01
R1853:Zfp51	UTSW	17	21,464,323 (GRCm38)	missense	probably damaging	1.00
R1989:Zfp51	UTSW	17	21,456,320 (GRCm38)	missense	possibly damaging	0.90
R2285:Zfp51	UTSW	17	21,463,875 (GRCm38)	missense	probably damaging	1.00
R2407:Zfp51	UTSW	17	21,463,831 (GRCm38)	missense	probably damaging	0.98
R2890:Zfp51	UTSW	17	21,463,856 (GRCm38)	missense	probably damaging	1.00
R3918:Zfp51	UTSW	17	21,463,440 (GRCm38)	missense	probably benign
R4529:Zfp51	UTSW	17	21,464,736 (GRCm38)	missense	probably damaging	1.00
R4587:Zfp51	UTSW	17	21,464,916 (GRCm38)	nonsense	probably null
R4866:Zfp51	UTSW	17	21,461,750 (GRCm38)	missense	possibly damaging	0.61
R4872:Zfp51	UTSW	17	21,464,671 (GRCm38)	missense	probably benign	0.26
R4961:Zfp51	UTSW	17	21,456,353 (GRCm38)	missense	probably benign	0.01
R5392:Zfp51	UTSW	17	21,465,322 (GRCm38)	missense	possibly damaging	0.60
R5611:Zfp51	UTSW	17	21,464,092 (GRCm38)	missense	probably damaging	1.00
R7109:Zfp51	UTSW	17	21,463,569 (GRCm38)	missense	possibly damaging	0.80
R7129:Zfp51	UTSW	17	21,461,709 (GRCm38)	missense	probably damaging	1.00
R7269:Zfp51	UTSW	17	21,463,698 (GRCm38)	missense	probably benign	0.09
R7303:Zfp51	UTSW	17	21,463,796 (GRCm38)	missense	probably benign	0.24
R7514:Zfp51	UTSW	17	21,463,500 (GRCm38)	missense	probably benign	0.37
R8073:Zfp51	UTSW	17	21,464,032 (GRCm38)	missense	probably damaging	1.00
R8177:Zfp51	UTSW	17	21,463,867 (GRCm38)	missense	probably benign	0.05
R8560:Zfp51	UTSW	17	21,465,373 (GRCm38)	missense	probably benign	0.00
R8877:Zfp51	UTSW	17	21,461,755 (GRCm38)	missense	probably damaging	1.00
R9032:Zfp51	UTSW	17	21,464,398 (GRCm38)	missense	probably damaging	1.00
R9085:Zfp51	UTSW	17	21,464,398 (GRCm38)	missense	probably damaging	1.00
R9307:Zfp51	UTSW	17	21,464,471 (GRCm38)	missense	probably benign	0.30
R9489:Zfp51	UTSW	17	21,464,029 (GRCm38)	missense	probably damaging	1.00
R9605:Zfp51	UTSW	17	21,464,029 (GRCm38)	missense	probably damaging	1.00
R9686:Zfp51	UTSW	17	21,463,609 (GRCm38)	missense	probably damaging	1.00
R9735:Zfp51	UTSW	17	21,465,151 (GRCm38)	nonsense	probably null
R9795:Zfp51	UTSW	17	21,461,789 (GRCm38)	critical splice donor site	probably null
X0062:Zfp51	UTSW	17	21,464,995 (GRCm38)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CATAGAACAAGCCCTATTAATCTGAA -3'
(R):5'- AATGTAGGCTGTTGGTTGCA -3'

Sequencing Primer
(F):5'- CTGCATATGTGATACAGTCCATCAAC -3'
(R):5'- TTGCATGAGACTGTATGCAGAGC -3'

Posted On

2019-11-12