Mutagenetix > Incidental Mutation

Incidental Mutation 'R7670:Olfr368'

592080

Institutional Source

Beutler Lab

Gene Symbol

Olfr368

Ensembl Gene

ENSMUSG00000049018

Gene Name

olfactory receptor 368

Synonyms

MOR178-1, GA_x6K02T2NLDC-34015743-34016726

MMRRC Submission

045742-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.146) question?

Stock #

R7670 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

37326515-37332772 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 37331759 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Valine at position 4 (E4V)

Ref Sequence

ENSEMBL: ENSMUSP00000149348 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053990] [ENSMUST00000217299]

AlphaFold

Q8VF22

Predicted Effect

probably benign
Transcript: ENSMUST00000053990
AA Change: E4V

PolyPhen 2 Score 0.097 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000054082
Gene: ENSMUSG00000049018
AA Change: E4V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	35	311	2.2e-45	PFAM
Pfam:7tm_1	45	294	1.2e-16	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000217299
AA Change: E4V

PolyPhen 2 Score 0.097 (Sensitivity: 0.93; Specificity: 0.85)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

100% (62/62)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930435E12Rik	C	T	16: 38,828,091	D219N	possibly damaging	Het
Adam26a	T	A	8: 43,570,153	H100L	probably benign	Het
Adgrf2	T	C	17: 42,711,372	N187S	probably damaging	Het
Adipoq	T	A	16: 23,157,582	H244Q	probably damaging	Het
Arhgap40	T	A	2: 158,531,925	S209T	probably benign	Het
Arrdc3	C	T	13: 80,889,093	L123F	probably damaging	Het
Aspscr1	A	G	11: 120,689,039	N212D	probably benign	Het
Ccr9	A	T	9: 123,779,306	S18C	probably damaging	Het
Cdc42bpa	T	A	1: 180,065,081	V270D	probably damaging	Het
Clic4	A	G	4: 135,217,205	Y220H	probably damaging	Het
Cntln	A	C	4: 84,979,340	H388P	possibly damaging	Het
Col12a1	A	G	9: 79,631,643	V2457A	probably damaging	Het
Ctsk	A	G	3: 95,501,614	N103D	probably benign	Het
Ddx60	T	C	8: 61,975,792	S779P	probably damaging	Het
Dnah5	T	A	15: 28,246,232		probably null	Het
Dnah7b	T	A	1: 46,109,302	D279E	probably benign	Het
Fam117a	A	G	11: 95,378,834	N308S	probably benign	Het
Fasn	A	G	11: 120,813,419	V1419A	probably damaging	Het
Fhad1	A	G	4: 141,951,491	S625P	probably benign	Het
Gemin5	A	G	11: 58,147,928	V585A	probably benign	Het
Gm5145	C	A	17: 20,570,384	P8Q	probably benign	Het
Gm8332	T	C	12: 88,249,754	N116S	probably benign	Het
Herc1	C	A	9: 66,416,347	T1381K	probably damaging	Het
Herc6	T	C	6: 57,660,122	I824T	probably damaging	Het
Klrb1	C	T	6: 128,710,087	V161I	probably benign	Het
Krtap31-1	A	G	11: 99,908,432	N154D	not run	Het
Lcp1	A	T	14: 75,200,431	I94F	probably benign	Het
Lin7a	A	T	10: 107,382,691	Q154L	possibly damaging	Het
Lnx1	T	C	5: 74,685,690	Y33C	probably damaging	Het
Myo5b	T	C	18: 74,701,446	V859A	probably benign	Het
Ncbp1	A	G	4: 46,170,015	Q696R	probably damaging	Het
Neurl1b	C	G	17: 26,438,746	H219Q	probably benign	Het
Nme8	T	C	13: 19,658,829	E392G	probably benign	Het
Nufip1	CAAAACAGAAAACAGAAAAC	CAAAACAGAAAACAGAAAACAGAAAAC	14: 76,111,974		probably null	Het
Nuggc	A	G	14: 65,613,526	I298V	probably damaging	Het
Nup155	A	C	15: 8,153,696	K1247Q	probably damaging	Het
Olfr403	A	G	11: 74,196,207	K235E	probably damaging	Het
Otud4	T	A	8: 79,655,864		probably null	Het
Pcdhb18	T	C	18: 37,491,696	V693A	probably damaging	Het
Pcnx3	A	G	19: 5,677,182	F1108L	probably benign	Het
Prkca	A	T	11: 108,014,344	N189K	probably damaging	Het
Rbm24	A	G	13: 46,429,207	I201V	probably benign	Het
Reep6	T	C	10: 80,333,793	L105P	probably damaging	Het
Retreg1	T	C	15: 25,941,040		probably benign	Het
Rev3l	C	T	10: 39,836,722	T2382I	probably benign	Het
Rnf31	A	G	14: 55,594,361	N230S	probably benign	Het
Rreb1	T	C	13: 37,931,572	L969P	probably benign	Het
Rsph4a	T	A	10: 33,909,033	N313K	probably damaging	Het
Serpina3f	T	A	12: 104,217,266	L129Q	probably damaging	Het
Slc9a2	T	A	1: 40,718,997	V232D	probably damaging	Het
Stmn2	T	C	3: 8,554,865	L121P	probably damaging	Het
Svep1	C	T	4: 58,097,424	G1373D	probably damaging	Het
Tmem206	A	G	1: 191,340,868	N162S	probably benign	Het
Tns1	C	A	1: 73,952,477	R1014L	possibly damaging	Het
Top2b	T	C	14: 16,416,620	S1127P	possibly damaging	Het
Txndc16	A	T	14: 45,135,867	C768*	probably null	Het
Ubl7	A	T	9: 57,929,769	E354D	probably benign	Het
Ush2a	T	C	1: 188,784,708	L3205P	possibly damaging	Het
Xirp2	A	G	2: 67,510,573	T1053A	possibly damaging	Het
Zbtb21	A	G	16: 97,951,877	L402P	probably damaging	Het
Zfp27	T	A	7: 29,894,796	K581N	possibly damaging	Het
Zfp62	A	T	11: 49,215,076		probably benign	Het

Other mutations in Olfr368

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01597:Olfr368	APN	2	37332011	missense	possibly damaging	0.60
IGL02955:Olfr368	APN	2	37332001	missense	probably benign	0.07
IGL03028:Olfr368	APN	2	37331956	missense	probably damaging	1.00
IGL03084:Olfr368	APN	2	37332401	missense	probably damaging	1.00
R1626:Olfr368	UTSW	2	37332762	splice site	probably null
R1655:Olfr368	UTSW	2	37331939	missense	probably damaging	1.00
R1771:Olfr368	UTSW	2	37332418	missense	probably benign	0.23
R1937:Olfr368	UTSW	2	37331884	missense	probably damaging	1.00
R2297:Olfr368	UTSW	2	37332143	missense	probably benign	0.04
R4354:Olfr368	UTSW	2	37331876	missense	probably damaging	1.00
R4888:Olfr368	UTSW	2	37332589	missense	probably damaging	1.00
R4974:Olfr368	UTSW	2	37332566	missense	probably damaging	1.00
R5121:Olfr368	UTSW	2	37332589	missense	probably damaging	1.00
R6144:Olfr368	UTSW	2	37332113	missense	probably damaging	0.97
R6449:Olfr368	UTSW	2	37331825	missense	possibly damaging	0.93
R8049:Olfr368	UTSW	2	37332334	missense	probably damaging	1.00
R8486:Olfr368	UTSW	2	37332650	missense	probably damaging	1.00
R8893:Olfr368	UTSW	2	37332376	missense	probably damaging	1.00
R9147:Olfr368	UTSW	2	37332005	missense	possibly damaging	0.68
R9148:Olfr368	UTSW	2	37332005	missense	possibly damaging	0.68
R9401:Olfr368	UTSW	2	37332281	missense	possibly damaging	0.94
R9574:Olfr368	UTSW	2	37332136	missense	possibly damaging	0.92
Z1177:Olfr368	UTSW	2	37332004	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAAGGGTTCCTTTAAACAAACCTC -3'
(R):5'- TGGAGCCTGGCATCTATACG -3'

Sequencing Primer
(F):5'- CCTGCAGATTTCTCAGTGTTAGGAAC -3'
(R):5'- CCTGGCATCTATACGAATGAGTAGC -3'

Posted On

2019-11-12