Mutagenetix > Incidental Mutation

Incidental Mutation 'R7671:Mgat4f'

592139

Institutional Source

Beutler Lab

Gene Symbol

Mgat4f

Ensembl Gene

ENSMUSG00000050526

Gene Name

MGAT4 family, member F

Synonyms

4933406M09Rik

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7671 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

134313678-134318719 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 134317800 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Arginine at position 191 (C191R)

Ref Sequence

ENSEMBL: ENSMUSP00000124251 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000162187]

AlphaFold

G3XA12

Predicted Effect

probably benign
Transcript: ENSMUST00000162187
AA Change: C191R

PolyPhen 2 Score 0.272 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000124251
Gene: ENSMUSG00000050526
AA Change: C191R

Domain	Start	End	E-Value	Type
Pfam:Glyco_transf_54	52	326	7.6e-79	PFAM
low complexity region	395	405	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

Allele List at MGI

All alleles(6) : Targeted(2) Gene trapped(4)

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akap13	C	A	7: 75,219,648 (GRCm39)	T17K	probably damaging	Het
Ankrd29	T	A	18: 12,394,043 (GRCm39)	K257I	probably damaging	Het
Bicc1	A	G	10: 70,792,997 (GRCm39)	L219P	probably benign	Het
Bltp1	T	C	3: 36,997,380 (GRCm39)	F1146L	probably damaging	Het
Boc	C	T	16: 44,312,212 (GRCm39)	V617M		Het
Ccl8	A	G	11: 82,006,033 (GRCm39)		probably benign	Het
Col18a1	A	G	10: 76,921,217 (GRCm39)	L261P	unknown	Het
Col5a3	T	A	9: 20,686,382 (GRCm39)		probably null	Het
Cryba4	A	C	5: 112,396,039 (GRCm39)		probably null	Het
Csf2rb	A	G	15: 78,223,130 (GRCm39)	Y114C	probably damaging	Het
Ctif	T	C	18: 75,605,087 (GRCm39)	D484G	probably damaging	Het
Ctnnal1	A	G	4: 56,837,848 (GRCm39)	F261L	probably damaging	Het
Defb22	A	T	2: 152,327,950 (GRCm39)	N78K	unknown	Het
Dele1	T	A	18: 38,392,978 (GRCm39)	I394N	probably damaging	Het
Efr3a	G	A	15: 65,709,283 (GRCm39)		probably null	Het
Elp4	G	T	2: 105,734,826 (GRCm39)	A3E	probably damaging	Het
Emilin2	A	G	17: 71,580,905 (GRCm39)	V607A	probably benign	Het
Epsti1	A	G	14: 78,141,930 (GRCm39)	Y2C	probably damaging	Het
Fam81b	A	C	13: 76,419,412 (GRCm39)	L46R	probably damaging	Het
Fam81b	G	T	13: 76,419,413 (GRCm39)	L46I	possibly damaging	Het
Fbxo15	T	A	18: 84,982,278 (GRCm39)	H288Q	probably damaging	Het
Gabrg1	T	A	5: 70,973,323 (GRCm39)	N77I	probably damaging	Het
Ganab	A	G	19: 8,890,216 (GRCm39)	Y715C	possibly damaging	Het
H2bc27	T	C	11: 58,840,102 (GRCm39)	S113P	possibly damaging	Het
Iqgap2	T	C	13: 95,764,627 (GRCm39)	D1539G	probably damaging	Het
Kcp	T	C	6: 29,496,516 (GRCm39)	N633S	probably benign	Het
Krt6a	C	A	15: 101,598,978 (GRCm39)	S529I	unknown	Het
Leo1	A	G	9: 75,352,844 (GRCm39)	H129R	probably benign	Het
Lmf1	G	A	17: 25,798,323 (GRCm39)	V55M	possibly damaging	Het
Mcm9	A	G	10: 53,413,665 (GRCm39)	S472P	probably benign	Het
Med27	T	A	2: 29,267,950 (GRCm39)	V33D		Het
Mup15	C	T	4: 61,356,526 (GRCm39)	E80K	probably benign	Het
Myo18a	G	A	11: 77,750,246 (GRCm39)	R199H		Het
Nebl	T	A	2: 17,395,727 (GRCm39)	R558*	probably null	Het
Or10ak8	A	T	4: 118,774,183 (GRCm39)	H160Q	probably benign	Het
Or11g7	G	T	14: 50,691,342 (GRCm39)	V278L	probably benign	Het
Or12e9	A	G	2: 87,202,613 (GRCm39)	T246A	probably damaging	Het
Or4a79	A	T	2: 89,551,603 (GRCm39)	I284K	probably damaging	Het
Or5p78	T	C	7: 108,212,198 (GRCm39)	I228T	probably damaging	Het
Or6c217	T	C	10: 129,738,222 (GRCm39)	D119G	probably damaging	Het
Or8b41	A	T	9: 38,054,736 (GRCm39)	M97L	probably benign	Het
Pde11a	A	T	2: 76,045,697 (GRCm39)	F376I	possibly damaging	Het
Prima1	A	T	12: 103,201,920 (GRCm39)	C52S	probably damaging	Het
Pxn	G	A	5: 115,686,606 (GRCm39)	R366H	not run	Het
Sgce	T	C	6: 4,691,564 (GRCm39)	Y337C	probably damaging	Het
Slc25a10	T	A	11: 120,386,286 (GRCm39)	M130K	probably benign	Het
Slc29a2	A	T	19: 5,074,290 (GRCm39)	N5I	probably benign	Het
Slc35a1	A	T	4: 34,673,875 (GRCm39)	H150Q		Het
Slc5a4a	T	A	10: 75,983,384 (GRCm39)	V7D	unknown	Het
Stk17b	T	A	1: 53,805,159 (GRCm39)	D134V	probably damaging	Het
Tex55	C	T	16: 38,648,453 (GRCm39)	D219N	possibly damaging	Het
Thbs3	T	A	3: 89,124,014 (GRCm39)	S36T	probably benign	Het
Thsd4	A	G	9: 60,335,457 (GRCm39)	S152P	probably benign	Het
Tmem67	G	T	4: 12,063,698 (GRCm39)	H422N	probably benign	Het
Tram1	C	A	1: 13,659,868 (GRCm39)	V27F	probably damaging	Het
Trim14	A	G	4: 46,507,238 (GRCm39)	V326A	possibly damaging	Het

Other mutations in Mgat4f

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01554:Mgat4f	APN	1	134,317,696 (GRCm39)	missense	probably damaging	1.00
IGL01862:Mgat4f	APN	1	134,318,349 (GRCm39)	missense	probably benign	0.03
P0005:Mgat4f	UTSW	1	134,315,646 (GRCm39)	missense	probably benign	0.00
R0498:Mgat4f	UTSW	1	134,318,610 (GRCm39)	missense	possibly damaging	0.69
R0563:Mgat4f	UTSW	1	134,317,777 (GRCm39)	missense	probably benign	0.00
R0731:Mgat4f	UTSW	1	134,317,713 (GRCm39)	missense	probably benign
R1558:Mgat4f	UTSW	1	134,318,512 (GRCm39)	missense	probably damaging	1.00
R2146:Mgat4f	UTSW	1	134,318,251 (GRCm39)	missense	probably damaging	1.00
R2148:Mgat4f	UTSW	1	134,318,251 (GRCm39)	missense	probably damaging	1.00
R2901:Mgat4f	UTSW	1	134,318,662 (GRCm39)	missense	probably damaging	0.99
R3897:Mgat4f	UTSW	1	134,318,176 (GRCm39)	missense	possibly damaging	0.92
R4543:Mgat4f	UTSW	1	134,317,531 (GRCm39)	missense	probably benign	0.31
R4937:Mgat4f	UTSW	1	134,317,714 (GRCm39)	missense	probably benign	0.00
R5490:Mgat4f	UTSW	1	134,317,666 (GRCm39)	missense	probably damaging	1.00
R5684:Mgat4f	UTSW	1	134,317,660 (GRCm39)	missense	probably benign	0.04
R5823:Mgat4f	UTSW	1	134,318,655 (GRCm39)	missense	probably damaging	0.98
R6488:Mgat4f	UTSW	1	134,318,626 (GRCm39)	missense	probably damaging	1.00
R7177:Mgat4f	UTSW	1	134,318,163 (GRCm39)	missense	probably benign	0.08
R7201:Mgat4f	UTSW	1	134,318,206 (GRCm39)	missense	possibly damaging	0.69
R7749:Mgat4f	UTSW	1	134,318,250 (GRCm39)	missense	probably benign	0.45
R8385:Mgat4f	UTSW	1	134,318,376 (GRCm39)	missense	probably benign	0.00
R9393:Mgat4f	UTSW	1	134,318,596 (GRCm39)	missense	probably benign	0.02
Z1177:Mgat4f	UTSW	1	134,317,896 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGACACCATGACATCCTTGTTC -3'
(R):5'- TGGAGAAGTCTAGGCTCACC -3'

Sequencing Primer
(F):5'- GTTCCAAGCTTCCTCGGAACAG -3'
(R):5'- TCACCCAAGGGAGCTGTTTC -3'

Posted On

2019-11-12