|Institutional Source||Beutler Lab|
|Gene Name||histone cluster 3, H2ba|
|Essential gene?||Probably non essential (E-score: 0.185)|
|Stock #||R7671 (G1)|
|Chromosomal Location||58948920-58949533 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 58949276 bp (GRCm38)|
|Amino Acid Change||Serine to Proline at position 113 (S113P)|
|Ref Sequence||ENSEMBL: ENSMUSP00000076397 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000078267]|
Crystal structure of the nucleosome core particle containing the histone domain of macroH2A [X-RAY DIFFRACTION]
2.9 Angstrom X-ray structure of hybrid macroH2A nucleosomes [X-RAY DIFFRACTION]
AA Change: S113P
PolyPhen 2 Score 0.705 (Sensitivity: 0.86; Specificity: 0.92)
AA Change: S113P
|Coding Region Coverage||
|MGI Phenotype||FUNCTION: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. [provided by RefSeq, Sep 2015]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Hist3h2ba||
(F):5'- TACGTGTACAAGGTGCTGAAG -3'
(R):5'- AGGATCCCAAGGTCCAGTAC -3'
(F):5'- TGAAGCAGGTGCATCCG -3'
(R):5'- GGATCCCAAGGTCCAGTACAACAC -3'