Mutagenetix > Incidental Mutation

Incidental Mutation 'R7680:Vmn2r78'

592698

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r78

Ensembl Gene

ENSMUSG00000091962

Gene Name

vomeronasal 2, receptor 78

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.064) question?

Stock #

R7680 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

86915300-86955177 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 86954941 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 776 (T776A)

Ref Sequence

ENSEMBL: ENSMUSP00000126698 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170835]

AlphaFold

K7N6U5

Predicted Effect

probably damaging
Transcript: ENSMUST00000170835
AA Change: T776A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000126698
Gene: ENSMUSG00000091962
AA Change: T776A

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	75	464	5.9e-31	PFAM
Pfam:NCD3G	507	559	8.1e-21	PFAM
Pfam:7tm_3	592	827	1e-52	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

100% (53/53)

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2510002D24Rik	A	G	16: 18,839,208	D104G	possibly damaging	Het
Ahi1	G	T	10: 21,007,768	C844F	possibly damaging	Het
AI464131	C	A	4: 41,497,978	D551Y	probably damaging	Het
B230118H07Rik	T	C	2: 101,610,556	E34G	probably damaging	Het
Bspry	T	C	4: 62,496,591	*474Q	probably null	Het
Car9	T	A	4: 43,507,250	D65E	probably damaging	Het
Ccdc110	T	A	8: 45,941,651	M193K	possibly damaging	Het
Ccdc166	A	G	15: 75,981,207	S304P	possibly damaging	Het
Cdcp1	T	C	9: 123,183,519	Q321R	probably damaging	Het
Cic	T	C	7: 25,292,431	V2255A	probably damaging	Het
Cmc2	A	T	8: 116,894,110	M44K	probably damaging	Het
Crls1	A	G	2: 132,862,338	T223A	probably damaging	Het
Ctnna3	A	T	10: 64,487,550	H488L	probably benign	Het
Cyp2r1	A	T	7: 114,552,819	I301N	probably damaging	Het
Dkk3	A	T	7: 112,119,363	L272Q	probably damaging	Het
Dnah14	A	G	1: 181,685,800	N1906S	probably benign	Het
Gm13271	T	C	4: 88,755,130	V88A	probably benign	Het
Gphn	C	A	12: 78,412,374	L79I	probably benign	Het
Gpr15	A	T	16: 58,717,965	W254R	probably damaging	Het
H2-M2	T	C	17: 37,483,025	R103G	possibly damaging	Het
Hcn4	T	C	9: 58,860,671	S1172P	probably benign	Het
Htr1a	A	G	13: 105,445,031	S260G	probably benign	Het
Kif21b	G	T	1: 136,147,869		probably null	Het
Lamp1	T	C	8: 13,167,812	Y131H	probably benign	Het
Lrrc47	A	C	4: 154,016,101	N378T	probably benign	Het
Manea	A	T	4: 26,340,649	H104Q	probably damaging	Het
Map4k3	A	G	17: 80,581,876	I888T	probably benign	Het
Mark3	T	C	12: 111,646,773	M557T	probably benign	Het
Muc6	G	C	7: 141,637,746	P2338R	probably damaging	Het
Myh6	C	A	14: 54,948,733	C1413F	possibly damaging	Het
Ncr1	T	A	7: 4,338,124	M38K	possibly damaging	Het
Nid2	A	G	14: 19,779,647	T669A	probably damaging	Het
Olfr49	T	A	14: 54,282,380	I172F	probably damaging	Het
Plat	G	T	8: 22,772,232	G91W	probably damaging	Het
Plekha7	A	G	7: 116,164,276	Y364H	probably benign	Het
Plxnb1	T	A	9: 109,100,503	Y142*	probably null	Het
Ptprn	T	C	1: 75,247,893	I940V	probably benign	Het
Rnf213	T	C	11: 119,479,556	V4728A		Het
Samd9l	A	T	6: 3,372,569	L1564Q	probably damaging	Het
Samd9l	A	G	6: 3,376,469	I264T	probably damaging	Het
Slc16a7	T	C	10: 125,230,936	D278G	probably benign	Het
Slc35f2	T	A	9: 53,808,112	V214E	probably damaging	Het
Slc7a5	A	C	8: 121,907,267	S114A	probably damaging	Het
St6galnac3	A	G	3: 153,205,410	S305P	probably damaging	Het
Stat1	G	A	1: 52,144,209	R378Q	probably damaging	Het
Tnfrsf9	T	G	4: 150,929,938	C31W	probably damaging	Het
Tnk1	T	C	11: 69,856,745	E61G	possibly damaging	Het
Tnks1bp1	T	A	2: 85,059,241	D637E	probably benign	Het
Ttc17	C	T	2: 94,366,544	G486D	probably benign	Het
Vmn1r30	T	A	6: 58,435,299	R183*	probably null	Het
Wrap73	A	G	4: 154,156,622	E444G	probably benign	Het
Zc3h13	C	T	14: 75,330,515	R1083C	probably damaging	Het
Zfp189	A	G	4: 49,521,547		probably benign	Het

Other mutations in Vmn2r78

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01302:Vmn2r78	APN	7	86915361	missense	unknown
IGL01473:Vmn2r78	APN	7	86920312	missense	possibly damaging	0.61
IGL01767:Vmn2r78	APN	7	86954435	missense	probably benign	0.28
IGL02322:Vmn2r78	APN	7	86921479	missense	probably damaging	0.96
IGL02537:Vmn2r78	APN	7	86954288	missense	probably damaging	0.99
IGL03297:Vmn2r78	APN	7	86920761	nonsense	probably null
ANU74:Vmn2r78	UTSW	7	86921065	missense	possibly damaging	0.62
R0035:Vmn2r78	UTSW	7	86920205	missense	probably benign	0.22
R0081:Vmn2r78	UTSW	7	86923027	missense	probably benign	0.35
R0401:Vmn2r78	UTSW	7	86921311	missense	probably benign	0.04
R0751:Vmn2r78	UTSW	7	86954380	missense	possibly damaging	0.77
R1341:Vmn2r78	UTSW	7	86922269	missense	possibly damaging	0.71
R1386:Vmn2r78	UTSW	7	86915407	missense	unknown
R1526:Vmn2r78	UTSW	7	86922257	splice site	probably null
R1712:Vmn2r78	UTSW	7	86954924	missense	probably damaging	1.00
R1739:Vmn2r78	UTSW	7	86920789	missense	probably benign
R1812:Vmn2r78	UTSW	7	86920787	missense	probably benign	0.38
R2011:Vmn2r78	UTSW	7	86955079	missense	possibly damaging	0.52
R2144:Vmn2r78	UTSW	7	86954482	missense	probably damaging	1.00
R2197:Vmn2r78	UTSW	7	86921327	missense	probably damaging	0.96
R2291:Vmn2r78	UTSW	7	86920154	missense	probably damaging	1.00
R2409:Vmn2r78	UTSW	7	86920745	splice site	probably benign
R3023:Vmn2r78	UTSW	7	86954966	missense	probably damaging	1.00
R4486:Vmn2r78	UTSW	7	86920751	critical splice acceptor site	probably null
R4512:Vmn2r78	UTSW	7	86920244	missense	probably benign	0.00
R4515:Vmn2r78	UTSW	7	86954258	missense	probably damaging	0.99
R4544:Vmn2r78	UTSW	7	86921191	missense	probably benign
R4546:Vmn2r78	UTSW	7	86954603	missense	probably damaging	1.00
R4872:Vmn2r78	UTSW	7	86954708	missense	possibly damaging	0.87
R4928:Vmn2r78	UTSW	7	86954627	missense	probably damaging	1.00
R5101:Vmn2r78	UTSW	7	86922355	missense	probably damaging	1.00
R5265:Vmn2r78	UTSW	7	86920124	missense	probably damaging	1.00
R5328:Vmn2r78	UTSW	7	86921030	missense	probably damaging	0.98
R5442:Vmn2r78	UTSW	7	86920122	missense	possibly damaging	0.95
R5567:Vmn2r78	UTSW	7	86921529	missense	probably benign	0.17
R5572:Vmn2r78	UTSW	7	86915512	missense	probably benign	0.01
R5636:Vmn2r78	UTSW	7	86954429	missense	probably damaging	0.99
R5901:Vmn2r78	UTSW	7	86954588	missense	probably damaging	1.00
R5977:Vmn2r78	UTSW	7	86920333	missense	possibly damaging	0.74
R5977:Vmn2r78	UTSW	7	86954907	missense	probably benign	0.00
R6276:Vmn2r78	UTSW	7	86921110	missense	probably benign	0.00
R6386:Vmn2r78	UTSW	7	86922337	nonsense	probably null
R6724:Vmn2r78	UTSW	7	86954258	missense	probably damaging	0.99
R6852:Vmn2r78	UTSW	7	86954603	missense	probably damaging	1.00
R6896:Vmn2r78	UTSW	7	86922350	missense	probably benign	0.10
R7385:Vmn2r78	UTSW	7	86922425	missense	probably benign	0.18
R7578:Vmn2r78	UTSW	7	86954344	nonsense	probably null
R7748:Vmn2r78	UTSW	7	86921135	missense	probably benign	0.00
R7852:Vmn2r78	UTSW	7	86920170	nonsense	probably null
R8031:Vmn2r78	UTSW	7	86954867	missense	probably damaging	1.00
R8070:Vmn2r78	UTSW	7	86922487	missense	probably benign	0.01
R8085:Vmn2r78	UTSW	7	86954790	missense	probably benign	0.00
R8163:Vmn2r78	UTSW	7	86954452	missense	probably damaging	1.00
R8501:Vmn2r78	UTSW	7	86920886	missense	probably damaging	0.99
R8749:Vmn2r78	UTSW	7	86954305	missense	possibly damaging	0.81
R9209:Vmn2r78	UTSW	7	86920223	missense	probably benign	0.08
RF018:Vmn2r78	UTSW	7	86954431	nonsense	probably null
Z1177:Vmn2r78	UTSW	7	86921207	missense	probably benign	0.44
Z1177:Vmn2r78	UTSW	7	86954774	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- AGGTGGCTGCTACTTTCAG -3'
(R):5'- GTGGTCTCAACAAAATAAGGTAGC -3'

Sequencing Primer
(F):5'- GAATCTGGCTGGGAACTTCTCC -3'
(R):5'- CAAAATAAGGTAGCACTTTGGCAC -3'

Posted On

2019-11-12