Mutagenetix > Incidental Mutation

Incidental Mutation 'R0240:Nfasc'

59270

Institutional Source

Beutler Lab

Gene Symbol

Nfasc

Ensembl Gene

ENSMUSG00000026442

Gene Name

neurofascin

Synonyms

D430023G06Rik

MMRRC Submission

038478-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0240 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

132492428-132669535 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 132529721 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 814 (S814P)

Ref Sequence

ENSEMBL: ENSMUSP00000139520 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000043189] [ENSMUST00000094569] [ENSMUST00000163770] [ENSMUST00000187861] [ENSMUST00000188307]

AlphaFold

Q810U3

Predicted Effect

probably damaging
Transcript: ENSMUST00000043189
AA Change: S799P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000035454
Gene: ENSMUSG00000026442
AA Change: S799P

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
IG	42	131	4.5e0	SMART
IG	141	228	2.44e-7	SMART
IGc2	253	317	1.53e-17	SMART
IGc2	343	409	1.76e-8	SMART
IGc2	437	502	2.39e-10	SMART
IGc2	528	593	2.54e-5	SMART
FN3	607	690	2.17e-11	SMART
FN3	707	789	2.85e-6	SMART
FN3	805	896	2.21e-3	SMART
FN3	911	995	9.92e-6	SMART
low complexity region	996	1018	N/A	INTRINSIC
transmembrane domain	1026	1048	N/A	INTRINSIC
Pfam:Bravo_FIGEY	1049	1133	1.4e-29	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000094569
AA Change: S820P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000092148
Gene: ENSMUSG00000026442
AA Change: S820P

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
IG	48	137	4.5e0	SMART
IG	147	234	2.44e-7	SMART
IGc2	259	323	1.53e-17	SMART
IGc2	349	415	1.76e-8	SMART
IGc2	443	508	2.39e-10	SMART
IGc2	534	599	2.54e-5	SMART
FN3	628	711	2.17e-11	SMART
FN3	728	810	2.85e-6	SMART
FN3	825	909	9.92e-6	SMART
FN3	1010	1086	6.91e-5	SMART
transmembrane domain	1109	1131	N/A	INTRINSIC
Pfam:Bravo_FIGEY	1132	1216	2.2e-29	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000163770
AA Change: S816P

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000132979
Gene: ENSMUSG00000026442
AA Change: S816P

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
IG	42	131	4.5e0	SMART
IG	141	228	2.44e-7	SMART
IGc2	270	334	1.53e-17	SMART
IGc2	360	426	1.76e-8	SMART
IGc2	454	519	2.39e-10	SMART
IGc2	545	610	2.54e-5	SMART
FN3	624	707	2.17e-11	SMART
FN3	724	806	2.85e-6	SMART
FN3	822	913	2.21e-3	SMART
FN3	928	1012	9.92e-6	SMART
low complexity region	1013	1035	N/A	INTRINSIC
transmembrane domain	1043	1065	N/A	INTRINSIC
Pfam:Bravo_FIGEY	1066	1150	5e-30	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000186389
AA Change: S800P

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000186539

Predicted Effect

probably damaging
Transcript: ENSMUST00000187861
AA Change: S820P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000139955
Gene: ENSMUSG00000026442
AA Change: S820P

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
IG	48	137	1.8e-2	SMART
IG	147	234	1e-9	SMART
IGc2	259	323	6.4e-20	SMART
IGc2	349	415	7e-11	SMART
IGc2	443	508	9.7e-13	SMART
IGc2	534	599	1.1e-7	SMART
FN3	628	711	1e-13	SMART
FN3	728	810	1.4e-8	SMART
FN3	826	917	1.1e-5	SMART
FN3	932	1016	4.8e-8	SMART
FN3	1117	1193	3.4e-7	SMART
transmembrane domain	1216	1238	N/A	INTRINSIC
Pfam:Bravo_FIGEY	1239	1325	2.6e-26	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000188307
AA Change: S814P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000139520
Gene: ENSMUSG00000026442
AA Change: S814P

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
IG	42	131	1.8e-2	SMART
IG	141	228	1e-9	SMART
IGc2	253	317	6.4e-20	SMART
IGc2	343	409	7e-11	SMART
IGc2	437	502	9.7e-13	SMART
IGc2	528	593	1.1e-7	SMART
FN3	622	705	1e-13	SMART
FN3	722	804	1.4e-8	SMART
FN3	820	890	3.8e-1	SMART

Meta Mutation Damage Score

0.6529

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.6%
20x: 95.3%

Validation Efficiency

100% (112/112)

MGI Phenotype

FUNCTION: This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009]
PHENOTYPE: Mice homozygous for a null allele die within 6 to 7 days of birth, exhibit reduced nerve conduction velocity and abnormal paranodal junction formation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 110 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsf3	T	C	8: 123,506,920 (GRCm39)	L71P	probably damaging	Het
Adamts2	A	T	11: 50,666,201 (GRCm39)	D399V	probably damaging	Het
Adck2	T	A	6: 39,560,752 (GRCm39)	V380E	probably benign	Het
Alg11	T	A	8: 22,555,468 (GRCm39)	V243D	possibly damaging	Het
Ankrd27	T	A	7: 35,318,864 (GRCm39)	L585Q	probably damaging	Het
Armh4	A	G	14: 50,005,859 (GRCm39)		probably benign	Het
Atp7a	T	A	X: 105,153,447 (GRCm39)	N1117K	probably damaging	Het
Bltp3a	T	A	17: 28,114,844 (GRCm39)		probably benign	Het
Cacna1b	G	A	2: 24,528,669 (GRCm39)		probably benign	Het
Cacna1d	T	A	14: 29,818,926 (GRCm39)	M1210L	probably benign	Het
Cacna1s	T	C	1: 136,001,234 (GRCm39)		probably benign	Het
Chd7	T	C	4: 8,852,670 (GRCm39)		probably benign	Het
Col12a1	A	T	9: 79,559,315 (GRCm39)	S1858T	probably benign	Het
Cotl1	C	T	8: 120,567,063 (GRCm39)	W26*	probably null	Het
Csmd3	T	C	15: 47,492,635 (GRCm39)	T3000A	probably benign	Het
Dcp1a	T	A	14: 30,206,551 (GRCm39)		probably benign	Het
Ddhd2	A	T	8: 26,229,617 (GRCm39)		probably null	Het
Dnah8	T	C	17: 30,984,653 (GRCm39)	I3117T	probably damaging	Het
Dnm3	G	T	1: 162,181,194 (GRCm39)	Q162K	probably benign	Het
Dpy19l2	G	T	9: 24,569,876 (GRCm39)	A359D	probably damaging	Het
Ece1	T	A	4: 137,676,746 (GRCm39)		probably benign	Het
Eif4g3	A	G	4: 137,897,873 (GRCm39)	K1025R	probably damaging	Het
Eml2	C	A	7: 18,918,797 (GRCm39)	Y82*	probably null	Het
Eml6	A	G	11: 29,742,367 (GRCm39)	V1057A	possibly damaging	Het
Eral1	A	G	11: 77,966,884 (GRCm39)		probably benign	Het
Espl1	T	C	15: 102,220,976 (GRCm39)	S911P	probably benign	Het
Fbxo8	A	G	8: 57,043,296 (GRCm39)		probably benign	Het
Flrt1	A	T	19: 7,074,475 (GRCm39)		probably benign	Het
Fndc7	A	G	3: 108,766,235 (GRCm39)		probably benign	Het
G3bp1	G	A	11: 55,382,854 (GRCm39)	G139D	probably damaging	Het
Gabra6	C	T	11: 42,205,774 (GRCm39)	V351I	probably benign	Het
Galc	A	T	12: 98,218,293 (GRCm39)	H186Q	probably damaging	Het
Ganab	A	G	19: 8,890,177 (GRCm39)	D702G	possibly damaging	Het
Hdac10	T	C	15: 89,010,085 (GRCm39)	E291G	possibly damaging	Het
Hectd3	T	G	4: 116,859,810 (GRCm39)	V749G	probably damaging	Het
Kash5	C	T	7: 44,849,675 (GRCm39)	A83T	probably benign	Het
Kcnh1	T	A	1: 192,187,648 (GRCm39)	I703N	probably benign	Het
Kcnma1	G	A	14: 23,544,647 (GRCm39)	T505I	probably damaging	Het
Kctd11	A	G	11: 69,770,640 (GRCm39)	C133R	probably damaging	Het
Lama3	A	T	18: 12,672,880 (GRCm39)		probably null	Het
Lamb3	T	C	1: 193,017,335 (GRCm39)	L842P	probably damaging	Het
Ldlr	T	C	9: 21,649,295 (GRCm39)		probably benign	Het
Lipk	G	A	19: 34,024,210 (GRCm39)	R336H	probably benign	Het
Lrrc24	T	A	15: 76,607,409 (GRCm39)	D58V	probably damaging	Het
Lrsam1	A	G	2: 32,845,197 (GRCm39)	L106P	probably damaging	Het
Milr1	G	A	11: 106,645,722 (GRCm39)	W88*	probably null	Het
Mmp10	A	G	9: 7,506,544 (GRCm39)	D340G	probably damaging	Het
Mybpc1	T	A	10: 88,391,600 (GRCm39)	Y285F	possibly damaging	Het
Ncoa3	A	G	2: 165,896,320 (GRCm39)	T408A	probably benign	Het
Nefm	T	A	14: 68,358,583 (GRCm39)	K484*	probably null	Het
Nlrp4a	T	C	7: 26,161,941 (GRCm39)	V863A	probably benign	Het
Nos1	C	T	5: 118,005,948 (GRCm39)	P223S	probably benign	Het
Nr2f2	G	C	7: 70,009,923 (GRCm39)	P52R	probably damaging	Het
Or13c7	T	A	4: 43,854,512 (GRCm39)	S68T	probably damaging	Het
Or4c108	A	T	2: 88,803,740 (GRCm39)	L165Q	probably damaging	Het
Or5an6	A	T	19: 12,372,327 (GRCm39)	E233D	probably benign	Het
Or8k41	A	G	2: 86,313,730 (GRCm39)	S119P	possibly damaging	Het
Osbpl5	T	C	7: 143,295,406 (GRCm39)		probably null	Het
Otog	C	A	7: 45,913,456 (GRCm39)		probably null	Het
Pacs1	A	T	19: 5,206,402 (GRCm39)	I261N	possibly damaging	Het
Pbx1	G	A	1: 168,031,051 (GRCm39)	T189I	possibly damaging	Het
Pcnx1	T	C	12: 81,993,792 (GRCm39)	I908T	possibly damaging	Het
Pdxdc1	A	T	16: 13,697,309 (GRCm39)	W124R	probably damaging	Het
Phex	C	A	X: 155,969,214 (GRCm39)	D587Y	probably damaging	Het
Plcb3	A	T	19: 6,940,363 (GRCm39)	D435E	probably benign	Het
Plce1	A	C	19: 38,717,330 (GRCm39)	K1373T	probably damaging	Het
Prkcd	G	A	14: 30,324,045 (GRCm39)	A311V	probably damaging	Het
Ptpn3	A	T	4: 57,232,374 (GRCm39)	S421T	probably benign	Het
Ptprs	T	C	17: 56,743,087 (GRCm39)		probably null	Het
Qrich1	A	G	9: 108,411,333 (GRCm39)	D286G	probably damaging	Het
Rcc1	C	A	4: 132,060,226 (GRCm39)	G393V	probably damaging	Het
Reln	T	C	5: 22,311,043 (GRCm39)	N290S	probably benign	Het
Rgl1	T	C	1: 152,430,175 (GRCm39)		probably benign	Het
Rhpn1	C	T	15: 75,585,971 (GRCm39)	T628I	probably benign	Het
Rilp	A	G	11: 75,401,747 (GRCm39)	R176G	probably benign	Het
Riok3	C	T	18: 12,288,284 (GRCm39)	A487V	probably benign	Het
Rnf224	T	C	2: 25,126,219 (GRCm39)	T45A	probably damaging	Het
Rpa1	A	G	11: 75,219,513 (GRCm39)	V137A	probably benign	Het
Rps6ka1	C	A	4: 133,575,842 (GRCm39)	Q693H	probably benign	Het
Scn2a	G	T	2: 65,566,118 (GRCm39)	V1381F	probably benign	Het
Scp2	T	A	4: 107,955,275 (GRCm39)	H112L	probably benign	Het
Sdk1	T	C	5: 141,984,502 (GRCm39)	W696R	probably damaging	Het
Slc26a7	C	A	4: 14,532,651 (GRCm39)	V408F	probably damaging	Het
Slc28a2	T	A	2: 122,285,008 (GRCm39)	I332N	probably benign	Het
Slc37a3	A	G	6: 39,314,172 (GRCm39)	V480A	probably benign	Het
Slc45a4	T	A	15: 73,453,755 (GRCm39)	E674D	probably benign	Het
Smpd3	T	C	8: 106,991,788 (GRCm39)	E255G	probably damaging	Het
Snx29	C	T	16: 11,478,417 (GRCm39)	R658W	probably damaging	Het
Sppl2a	A	T	2: 126,762,256 (GRCm39)	M275K	probably benign	Het
Stac	T	C	9: 111,464,089 (GRCm39)	N59S	probably damaging	Het
Stk25	A	T	1: 93,554,782 (GRCm39)	L131Q	probably damaging	Het
Tep1	C	T	14: 51,100,486 (GRCm39)		probably benign	Het
Thbs1	C	A	2: 117,944,874 (GRCm39)	N229K	probably damaging	Het
Tmx2	A	T	2: 84,506,186 (GRCm39)	H89Q	probably damaging	Het
Tnfrsf21	C	T	17: 43,349,104 (GRCm39)	H239Y	probably benign	Het
Tradd	T	C	8: 105,985,924 (GRCm39)	N209S	possibly damaging	Het
Trappc3l	A	T	10: 33,974,928 (GRCm39)	R119*	probably null	Het
Trmt1l	G	A	1: 151,333,205 (GRCm39)		probably benign	Het
Ublcp1	G	T	11: 44,349,104 (GRCm39)	Y243*	probably null	Het
Usp24	C	A	4: 106,271,601 (GRCm39)	C2158*	probably null	Het
Usp34	A	T	11: 23,383,206 (GRCm39)	K2088N	probably damaging	Het
Vmn1r53	G	C	6: 90,200,925 (GRCm39)	S133C	probably damaging	Het
Vmn2r52	A	G	7: 9,893,327 (GRCm39)	V604A	probably damaging	Het
Vmn2r93	A	G	17: 18,525,061 (GRCm39)	K240E	probably benign	Het
Wdr13	T	G	X: 7,994,284 (GRCm39)	D242A	probably damaging	Het
Wwp1	C	T	4: 19,641,734 (GRCm39)		probably null	Het
Zan	G	A	5: 137,396,624 (GRCm39)	H4311Y	unknown	Het
Zc3h12c	C	A	9: 52,055,383 (GRCm39)	R123L	possibly damaging	Het
Zfp125	A	T	12: 20,950,562 (GRCm39)		noncoding transcript	Het
Zfp318	C	T	17: 46,707,739 (GRCm39)	P266S	probably benign	Het

Other mutations in Nfasc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00895:Nfasc	APN	1	132,501,536 (GRCm39)	nonsense	probably null
IGL01088:Nfasc	APN	1	132,570,514 (GRCm39)	utr 5 prime	probably benign
IGL01958:Nfasc	APN	1	132,536,176 (GRCm39)	nonsense	probably null
IGL01999:Nfasc	APN	1	132,532,985 (GRCm39)	splice site	probably benign
IGL02170:Nfasc	APN	1	132,538,104 (GRCm39)	nonsense	probably null
IGL02187:Nfasc	APN	1	132,498,219 (GRCm39)	missense	probably damaging	1.00
IGL02192:Nfasc	APN	1	132,498,219 (GRCm39)	missense	probably damaging	1.00
IGL02452:Nfasc	APN	1	132,548,662 (GRCm39)	critical splice donor site	probably null
IGL02698:Nfasc	APN	1	132,562,475 (GRCm39)	missense	probably benign	0.06
IGL02797:Nfasc	APN	1	132,538,186 (GRCm39)	missense	probably damaging	1.00
IGL03000:Nfasc	APN	1	132,549,247 (GRCm39)	splice site	probably benign
IGL03027:Nfasc	APN	1	132,538,207 (GRCm39)	missense	probably damaging	1.00
Fascist	UTSW	1	132,539,343 (GRCm39)	missense	probably damaging	1.00
jiggle	UTSW	1	132,529,759 (GRCm39)	missense	probably damaging	1.00
Partisan	UTSW	1	132,533,287 (GRCm39)	missense	probably damaging	1.00
Tremble	UTSW	1	132,539,333 (GRCm39)	missense	probably damaging	1.00
PIT4377001:Nfasc	UTSW	1	132,510,804 (GRCm39)	missense	unknown
R0240:Nfasc	UTSW	1	132,529,721 (GRCm39)	missense	probably damaging	1.00
R0241:Nfasc	UTSW	1	132,564,731 (GRCm39)	missense	probably benign	0.02
R0241:Nfasc	UTSW	1	132,564,731 (GRCm39)	missense	probably benign	0.02
R0418:Nfasc	UTSW	1	132,539,333 (GRCm39)	missense	probably damaging	1.00
R0513:Nfasc	UTSW	1	132,531,584 (GRCm39)	missense	possibly damaging	0.95
R0639:Nfasc	UTSW	1	132,531,554 (GRCm39)	missense	probably damaging	1.00
R0646:Nfasc	UTSW	1	132,536,176 (GRCm39)	nonsense	probably null
R1103:Nfasc	UTSW	1	132,534,795 (GRCm39)	splice site	probably benign
R1269:Nfasc	UTSW	1	132,538,526 (GRCm39)	missense	probably damaging	1.00
R1550:Nfasc	UTSW	1	132,536,241 (GRCm39)	missense	probably damaging	0.96
R1749:Nfasc	UTSW	1	132,539,370 (GRCm39)	missense	probably damaging	1.00
R1773:Nfasc	UTSW	1	132,538,577 (GRCm39)	missense	probably damaging	1.00
R1921:Nfasc	UTSW	1	132,538,543 (GRCm39)	missense	probably damaging	1.00
R1987:Nfasc	UTSW	1	132,538,624 (GRCm39)	missense	probably damaging	1.00
R2141:Nfasc	UTSW	1	132,524,383 (GRCm39)	missense	probably damaging	1.00
R2239:Nfasc	UTSW	1	132,510,760 (GRCm39)	intron	probably benign
R2413:Nfasc	UTSW	1	132,523,243 (GRCm39)	missense	probably damaging	1.00
R2428:Nfasc	UTSW	1	132,523,392 (GRCm39)	missense	possibly damaging	0.55
R2472:Nfasc	UTSW	1	132,515,959 (GRCm39)	intron	probably benign
R2517:Nfasc	UTSW	1	132,525,501 (GRCm39)	splice site	probably null
R3850:Nfasc	UTSW	1	132,559,471 (GRCm39)	missense	probably damaging	1.00
R4050:Nfasc	UTSW	1	132,538,043 (GRCm39)	splice site	probably benign
R4061:Nfasc	UTSW	1	132,525,583 (GRCm39)	missense	probably damaging	1.00
R4088:Nfasc	UTSW	1	132,523,329 (GRCm39)	missense	probably damaging	1.00
R4342:Nfasc	UTSW	1	132,559,443 (GRCm39)	missense	probably damaging	1.00
R4343:Nfasc	UTSW	1	132,559,443 (GRCm39)	missense	probably damaging	1.00
R4345:Nfasc	UTSW	1	132,559,443 (GRCm39)	missense	probably damaging	1.00
R4452:Nfasc	UTSW	1	132,562,409 (GRCm39)	missense	probably damaging	1.00
R4818:Nfasc	UTSW	1	132,531,568 (GRCm39)	missense	possibly damaging	0.87
R4851:Nfasc	UTSW	1	132,529,759 (GRCm39)	missense	probably damaging	1.00
R5014:Nfasc	UTSW	1	132,512,185 (GRCm39)	intron	probably benign
R5768:Nfasc	UTSW	1	132,532,883 (GRCm39)	missense	probably benign	0.00
R6145:Nfasc	UTSW	1	132,562,455 (GRCm39)	missense	probably damaging	1.00
R6335:Nfasc	UTSW	1	132,504,132 (GRCm39)	missense	probably damaging	0.98
R6379:Nfasc	UTSW	1	132,498,280 (GRCm39)	nonsense	probably null
R6486:Nfasc	UTSW	1	132,532,952 (GRCm39)	missense	probably damaging	1.00
R7022:Nfasc	UTSW	1	132,548,787 (GRCm39)	missense	probably damaging	1.00
R7062:Nfasc	UTSW	1	132,529,707 (GRCm39)	critical splice donor site	probably null
R7084:Nfasc	UTSW	1	132,498,247 (GRCm39)	missense	unknown
R7275:Nfasc	UTSW	1	132,562,001 (GRCm39)	missense	probably damaging	1.00
R7286:Nfasc	UTSW	1	132,529,790 (GRCm39)	missense	probably damaging	1.00
R7682:Nfasc	UTSW	1	132,501,511 (GRCm39)	missense	unknown
R7838:Nfasc	UTSW	1	132,533,287 (GRCm39)	missense	probably damaging	1.00
R7871:Nfasc	UTSW	1	132,527,751 (GRCm39)	missense	not run
R7938:Nfasc	UTSW	1	132,533,269 (GRCm39)	missense	probably damaging	1.00
R8083:Nfasc	UTSW	1	132,524,320 (GRCm39)	missense	probably benign	0.00
R8482:Nfasc	UTSW	1	132,532,827 (GRCm39)	missense	probably damaging	1.00
R9027:Nfasc	UTSW	1	132,539,343 (GRCm39)	missense	probably damaging	1.00
R9164:Nfasc	UTSW	1	132,562,544 (GRCm39)	missense	probably damaging	1.00
R9488:Nfasc	UTSW	1	132,527,866 (GRCm39)	missense	possibly damaging	0.68
R9651:Nfasc	UTSW	1	132,527,791 (GRCm39)	missense	probably benign	0.04
Z1176:Nfasc	UTSW	1	132,562,376 (GRCm39)	missense	probably benign	0.00
Z1177:Nfasc	UTSW	1	132,559,576 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCAGCACTGGGATGTCTATTGAGC -3'
(R):5'- CAGCCTATGAATGCTACCTCTGCC -3'

Sequencing Primer
(F):5'- AAAGGCAATTTTCCTCCTGACTG -3'
(R):5'- ATGAATGCTACCTCTGCCTTTGG -3'

Posted On

2013-07-11