Mutagenetix > Incidental Mutation

Incidental Mutation 'R0240:Rcc1'

59297

Institutional Source

Beutler Lab

Gene Symbol

Rcc1

Ensembl Gene

ENSMUSG00000028896

Gene Name

regulator of chromosome condensation 1

Synonyms

4931417M11Rik, Chc1

MMRRC Submission

038478-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.972) question?

Stock #

R0240 (G1)

Quality Score

148

Status

Validated

Chromosome

Chromosomal Location

132059230-132073061 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 132060226 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Valine at position 393 (G393V)

Ref Sequence

ENSEMBL: ENSMUSP00000101571 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030726] [ENSMUST00000030730] [ENSMUST00000084250] [ENSMUST00000105951] [ENSMUST00000105960] [ENSMUST00000105962] [ENSMUST00000125513] [ENSMUST00000127402] [ENSMUST00000147652] [ENSMUST00000155129] [ENSMUST00000137520]

AlphaFold

Q8VE37

Predicted Effect

probably damaging
Transcript: ENSMUST00000030726
AA Change: G406V

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000030726
Gene: ENSMUSG00000028896
AA Change: G406V

Domain	Start	End	E-Value	Type
Pfam:RCC1	47	95	7.9e-12	PFAM
Pfam:RCC1	98	147	7.5e-17	PFAM
Pfam:RCC1_2	134	165	1.3e-11	PFAM
Pfam:RCC1	150	200	9.9e-10	PFAM
Pfam:RCC1_2	187	216	3.2e-7	PFAM
Pfam:RCC1	203	268	4.2e-14	PFAM
Pfam:RCC1	271	322	1.1e-11	PFAM
Pfam:RCC1	325	373	3.4e-10	PFAM
Pfam:RCC1	376	427	3.4e-14	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000030730

SMART Domains

Protein: ENSMUSP00000030730
Gene: ENSMUSG00000028898

Domain	Start	End	E-Value	Type
RRM	4	78	3.66e-8	SMART
RRM	97	171	2.2e-11	SMART
low complexity region	185	204	N/A	INTRINSIC
low complexity region	213	229	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000084250
AA Change: G393V

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000081271
Gene: ENSMUSG00000028896
AA Change: G393V

Domain	Start	End	E-Value	Type
low complexity region	19	26	N/A	INTRINSIC
Pfam:RCC1	34	82	4.1e-10	PFAM
Pfam:RCC1	85	134	1.4e-13	PFAM
Pfam:RCC1_2	121	151	1.5e-8	PFAM
Pfam:RCC1	137	187	1.7e-7	PFAM
Pfam:RCC1_2	174	203	3e-5	PFAM
Pfam:RCC1	190	255	9.6e-11	PFAM
Pfam:RCC1	258	309	1.9e-9	PFAM
Pfam:RCC1	312	360	5.7e-9	PFAM
Pfam:RCC1	363	414	3.9e-12	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000105951
AA Change: G393V

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000101571
Gene: ENSMUSG00000028896
AA Change: G393V

Domain	Start	End	E-Value	Type
low complexity region	19	26	N/A	INTRINSIC
Pfam:RCC1	34	82	4.1e-10	PFAM
Pfam:RCC1	85	134	1.4e-13	PFAM
Pfam:RCC1_2	121	151	1.5e-8	PFAM
Pfam:RCC1	137	187	1.7e-7	PFAM
Pfam:RCC1_2	174	203	3e-5	PFAM
Pfam:RCC1	190	255	9.6e-11	PFAM
Pfam:RCC1	258	309	1.9e-9	PFAM
Pfam:RCC1	312	360	5.7e-9	PFAM
Pfam:RCC1	363	414	3.9e-12	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105960

SMART Domains

Protein: ENSMUSP00000101580
Gene: ENSMUSG00000028898

Domain	Start	End	E-Value	Type
PDB:2DHG\|A	21	70	9e-25	PDB
SCOP:d1fj7a_	22	70	7e-7	SMART
Blast:RRM	31	70	2e-21	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000105962

SMART Domains

Protein: ENSMUSP00000101582
Gene: ENSMUSG00000028898

Domain	Start	End	E-Value	Type
RRM	4	78	3.66e-8	SMART
PDB:2DHG\|A	87	137	7e-24	PDB
SCOP:d1cvja1	97	137	9e-5	SMART
Blast:RRM	97	138	2e-20	BLAST
low complexity region	145	164	N/A	INTRINSIC
low complexity region	173	189	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000125513

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000131524

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000146166

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155194

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141317

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150959

Predicted Effect

probably benign
Transcript: ENSMUST00000127402

SMART Domains

Protein: ENSMUSP00000120657
Gene: ENSMUSG00000028898

Domain	Start	End	E-Value	Type
RRM	4	78	3.66e-8	SMART
low complexity region	101	120	N/A	INTRINSIC
low complexity region	129	145	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000147652

SMART Domains

Protein: ENSMUSP00000127273
Gene: ENSMUSG00000028898

Domain	Start	End	E-Value	Type
RRM	4	78	1.73e-9	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000155129

SMART Domains

Protein: ENSMUSP00000120701
Gene: ENSMUSG00000028896

Domain	Start	End	E-Value	Type
low complexity region	19	26	N/A	INTRINSIC
Pfam:RCC1	34	82	3.9e-13	PFAM
Pfam:RCC1_2	69	98	5.2e-7	PFAM
Pfam:RCC1	85	116	5.3e-8	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000137520

Meta Mutation Damage Score

0.7977

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.6%
20x: 95.3%

Validation Efficiency

100% (112/112)

Allele List at MGI

Other mutations in this stock

Total: 110 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsf3	T	C	8: 123,506,920 (GRCm39)	L71P	probably damaging	Het
Adamts2	A	T	11: 50,666,201 (GRCm39)	D399V	probably damaging	Het
Adck2	T	A	6: 39,560,752 (GRCm39)	V380E	probably benign	Het
Alg11	T	A	8: 22,555,468 (GRCm39)	V243D	possibly damaging	Het
Ankrd27	T	A	7: 35,318,864 (GRCm39)	L585Q	probably damaging	Het
Armh4	A	G	14: 50,005,859 (GRCm39)		probably benign	Het
Atp7a	T	A	X: 105,153,447 (GRCm39)	N1117K	probably damaging	Het
Bltp3a	T	A	17: 28,114,844 (GRCm39)		probably benign	Het
Cacna1b	G	A	2: 24,528,669 (GRCm39)		probably benign	Het
Cacna1d	T	A	14: 29,818,926 (GRCm39)	M1210L	probably benign	Het
Cacna1s	T	C	1: 136,001,234 (GRCm39)		probably benign	Het
Chd7	T	C	4: 8,852,670 (GRCm39)		probably benign	Het
Col12a1	A	T	9: 79,559,315 (GRCm39)	S1858T	probably benign	Het
Cotl1	C	T	8: 120,567,063 (GRCm39)	W26*	probably null	Het
Csmd3	T	C	15: 47,492,635 (GRCm39)	T3000A	probably benign	Het
Dcp1a	T	A	14: 30,206,551 (GRCm39)		probably benign	Het
Ddhd2	A	T	8: 26,229,617 (GRCm39)		probably null	Het
Dnah8	T	C	17: 30,984,653 (GRCm39)	I3117T	probably damaging	Het
Dnm3	G	T	1: 162,181,194 (GRCm39)	Q162K	probably benign	Het
Dpy19l2	G	T	9: 24,569,876 (GRCm39)	A359D	probably damaging	Het
Ece1	T	A	4: 137,676,746 (GRCm39)		probably benign	Het
Eif4g3	A	G	4: 137,897,873 (GRCm39)	K1025R	probably damaging	Het
Eml2	C	A	7: 18,918,797 (GRCm39)	Y82*	probably null	Het
Eml6	A	G	11: 29,742,367 (GRCm39)	V1057A	possibly damaging	Het
Eral1	A	G	11: 77,966,884 (GRCm39)		probably benign	Het
Espl1	T	C	15: 102,220,976 (GRCm39)	S911P	probably benign	Het
Fbxo8	A	G	8: 57,043,296 (GRCm39)		probably benign	Het
Flrt1	A	T	19: 7,074,475 (GRCm39)		probably benign	Het
Fndc7	A	G	3: 108,766,235 (GRCm39)		probably benign	Het
G3bp1	G	A	11: 55,382,854 (GRCm39)	G139D	probably damaging	Het
Gabra6	C	T	11: 42,205,774 (GRCm39)	V351I	probably benign	Het
Galc	A	T	12: 98,218,293 (GRCm39)	H186Q	probably damaging	Het
Ganab	A	G	19: 8,890,177 (GRCm39)	D702G	possibly damaging	Het
Hdac10	T	C	15: 89,010,085 (GRCm39)	E291G	possibly damaging	Het
Hectd3	T	G	4: 116,859,810 (GRCm39)	V749G	probably damaging	Het
Kash5	C	T	7: 44,849,675 (GRCm39)	A83T	probably benign	Het
Kcnh1	T	A	1: 192,187,648 (GRCm39)	I703N	probably benign	Het
Kcnma1	G	A	14: 23,544,647 (GRCm39)	T505I	probably damaging	Het
Kctd11	A	G	11: 69,770,640 (GRCm39)	C133R	probably damaging	Het
Lama3	A	T	18: 12,672,880 (GRCm39)		probably null	Het
Lamb3	T	C	1: 193,017,335 (GRCm39)	L842P	probably damaging	Het
Ldlr	T	C	9: 21,649,295 (GRCm39)		probably benign	Het
Lipk	G	A	19: 34,024,210 (GRCm39)	R336H	probably benign	Het
Lrrc24	T	A	15: 76,607,409 (GRCm39)	D58V	probably damaging	Het
Lrsam1	A	G	2: 32,845,197 (GRCm39)	L106P	probably damaging	Het
Milr1	G	A	11: 106,645,722 (GRCm39)	W88*	probably null	Het
Mmp10	A	G	9: 7,506,544 (GRCm39)	D340G	probably damaging	Het
Mybpc1	T	A	10: 88,391,600 (GRCm39)	Y285F	possibly damaging	Het
Ncoa3	A	G	2: 165,896,320 (GRCm39)	T408A	probably benign	Het
Nefm	T	A	14: 68,358,583 (GRCm39)	K484*	probably null	Het
Nfasc	A	G	1: 132,529,721 (GRCm39)	S814P	probably damaging	Het
Nlrp4a	T	C	7: 26,161,941 (GRCm39)	V863A	probably benign	Het
Nos1	C	T	5: 118,005,948 (GRCm39)	P223S	probably benign	Het
Nr2f2	G	C	7: 70,009,923 (GRCm39)	P52R	probably damaging	Het
Or13c7	T	A	4: 43,854,512 (GRCm39)	S68T	probably damaging	Het
Or4c108	A	T	2: 88,803,740 (GRCm39)	L165Q	probably damaging	Het
Or5an6	A	T	19: 12,372,327 (GRCm39)	E233D	probably benign	Het
Or8k41	A	G	2: 86,313,730 (GRCm39)	S119P	possibly damaging	Het
Osbpl5	T	C	7: 143,295,406 (GRCm39)		probably null	Het
Otog	C	A	7: 45,913,456 (GRCm39)		probably null	Het
Pacs1	A	T	19: 5,206,402 (GRCm39)	I261N	possibly damaging	Het
Pbx1	G	A	1: 168,031,051 (GRCm39)	T189I	possibly damaging	Het
Pcnx1	T	C	12: 81,993,792 (GRCm39)	I908T	possibly damaging	Het
Pdxdc1	A	T	16: 13,697,309 (GRCm39)	W124R	probably damaging	Het
Phex	C	A	X: 155,969,214 (GRCm39)	D587Y	probably damaging	Het
Plcb3	A	T	19: 6,940,363 (GRCm39)	D435E	probably benign	Het
Plce1	A	C	19: 38,717,330 (GRCm39)	K1373T	probably damaging	Het
Prkcd	G	A	14: 30,324,045 (GRCm39)	A311V	probably damaging	Het
Ptpn3	A	T	4: 57,232,374 (GRCm39)	S421T	probably benign	Het
Ptprs	T	C	17: 56,743,087 (GRCm39)		probably null	Het
Qrich1	A	G	9: 108,411,333 (GRCm39)	D286G	probably damaging	Het
Reln	T	C	5: 22,311,043 (GRCm39)	N290S	probably benign	Het
Rgl1	T	C	1: 152,430,175 (GRCm39)		probably benign	Het
Rhpn1	C	T	15: 75,585,971 (GRCm39)	T628I	probably benign	Het
Rilp	A	G	11: 75,401,747 (GRCm39)	R176G	probably benign	Het
Riok3	C	T	18: 12,288,284 (GRCm39)	A487V	probably benign	Het
Rnf224	T	C	2: 25,126,219 (GRCm39)	T45A	probably damaging	Het
Rpa1	A	G	11: 75,219,513 (GRCm39)	V137A	probably benign	Het
Rps6ka1	C	A	4: 133,575,842 (GRCm39)	Q693H	probably benign	Het
Scn2a	G	T	2: 65,566,118 (GRCm39)	V1381F	probably benign	Het
Scp2	T	A	4: 107,955,275 (GRCm39)	H112L	probably benign	Het
Sdk1	T	C	5: 141,984,502 (GRCm39)	W696R	probably damaging	Het
Slc26a7	C	A	4: 14,532,651 (GRCm39)	V408F	probably damaging	Het
Slc28a2	T	A	2: 122,285,008 (GRCm39)	I332N	probably benign	Het
Slc37a3	A	G	6: 39,314,172 (GRCm39)	V480A	probably benign	Het
Slc45a4	T	A	15: 73,453,755 (GRCm39)	E674D	probably benign	Het
Smpd3	T	C	8: 106,991,788 (GRCm39)	E255G	probably damaging	Het
Snx29	C	T	16: 11,478,417 (GRCm39)	R658W	probably damaging	Het
Sppl2a	A	T	2: 126,762,256 (GRCm39)	M275K	probably benign	Het
Stac	T	C	9: 111,464,089 (GRCm39)	N59S	probably damaging	Het
Stk25	A	T	1: 93,554,782 (GRCm39)	L131Q	probably damaging	Het
Tep1	C	T	14: 51,100,486 (GRCm39)		probably benign	Het
Thbs1	C	A	2: 117,944,874 (GRCm39)	N229K	probably damaging	Het
Tmx2	A	T	2: 84,506,186 (GRCm39)	H89Q	probably damaging	Het
Tnfrsf21	C	T	17: 43,349,104 (GRCm39)	H239Y	probably benign	Het
Tradd	T	C	8: 105,985,924 (GRCm39)	N209S	possibly damaging	Het
Trappc3l	A	T	10: 33,974,928 (GRCm39)	R119*	probably null	Het
Trmt1l	G	A	1: 151,333,205 (GRCm39)		probably benign	Het
Ublcp1	G	T	11: 44,349,104 (GRCm39)	Y243*	probably null	Het
Usp24	C	A	4: 106,271,601 (GRCm39)	C2158*	probably null	Het
Usp34	A	T	11: 23,383,206 (GRCm39)	K2088N	probably damaging	Het
Vmn1r53	G	C	6: 90,200,925 (GRCm39)	S133C	probably damaging	Het
Vmn2r52	A	G	7: 9,893,327 (GRCm39)	V604A	probably damaging	Het
Vmn2r93	A	G	17: 18,525,061 (GRCm39)	K240E	probably benign	Het
Wdr13	T	G	X: 7,994,284 (GRCm39)	D242A	probably damaging	Het
Wwp1	C	T	4: 19,641,734 (GRCm39)		probably null	Het
Zan	G	A	5: 137,396,624 (GRCm39)	H4311Y	unknown	Het
Zc3h12c	C	A	9: 52,055,383 (GRCm39)	R123L	possibly damaging	Het
Zfp125	A	T	12: 20,950,562 (GRCm39)		noncoding transcript	Het
Zfp318	C	T	17: 46,707,739 (GRCm39)	P266S	probably benign	Het

Other mutations in Rcc1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02881:Rcc1	APN	4	132,065,067 (GRCm39)	missense	probably benign	0.20
IGL02927:Rcc1	APN	4	132,065,067 (GRCm39)	missense	probably benign	0.20
IGL02802:Rcc1	UTSW	4	132,065,067 (GRCm39)	missense	probably benign	0.20
IGL02837:Rcc1	UTSW	4	132,065,067 (GRCm39)	missense	probably benign	0.20
R0240:Rcc1	UTSW	4	132,060,226 (GRCm39)	missense	probably damaging	1.00
R0828:Rcc1	UTSW	4	132,063,136 (GRCm39)	unclassified	probably benign
R1606:Rcc1	UTSW	4	132,062,087 (GRCm39)	splice site	probably null
R2155:Rcc1	UTSW	4	132,065,360 (GRCm39)	critical splice donor site	probably null
R3721:Rcc1	UTSW	4	132,065,125 (GRCm39)	missense	possibly damaging	0.46
R4633:Rcc1	UTSW	4	132,063,080 (GRCm39)	missense	probably damaging	0.98
R4908:Rcc1	UTSW	4	132,065,064 (GRCm39)	missense	probably damaging	1.00
R4936:Rcc1	UTSW	4	132,063,046 (GRCm39)	missense	probably damaging	0.96
R5461:Rcc1	UTSW	4	132,061,497 (GRCm39)	missense	probably benign	0.00
R5627:Rcc1	UTSW	4	132,065,454 (GRCm39)	missense	probably damaging	0.98
R6088:Rcc1	UTSW	4	132,060,153 (GRCm39)	missense	probably benign	0.00
R6197:Rcc1	UTSW	4	132,065,073 (GRCm39)	missense	possibly damaging	0.58
R6456:Rcc1	UTSW	4	132,061,427 (GRCm39)	missense	probably benign	0.10
R7127:Rcc1	UTSW	4	132,062,107 (GRCm39)	missense	probably damaging	0.98
R7440:Rcc1	UTSW	4	132,065,110 (GRCm39)	missense	probably damaging	0.97
R7529:Rcc1	UTSW	4	132,061,874 (GRCm39)	missense	probably benign	0.00
R8168:Rcc1	UTSW	4	132,063,096 (GRCm39)	missense	probably benign	0.38
R8469:Rcc1	UTSW	4	132,061,445 (GRCm39)	missense	probably damaging	1.00
R8733:Rcc1	UTSW	4	132,065,515 (GRCm39)	missense	probably benign	0.03
R9454:Rcc1	UTSW	4	132,062,074 (GRCm39)	missense	probably damaging	1.00
R9483:Rcc1	UTSW	4	132,062,808 (GRCm39)	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- TAATGAGCCAGTGCCAGCTAGGAC -3'
(R):5'- AACAGAGATTCAGGACGTAGCCCC -3'

Sequencing Primer
(F):5'- GCAAAGATCCTTCTGAGGTCC -3'
(R):5'- GGACCTTGCCAGACTGCTATTAG -3'

Posted On

2013-07-11